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Transmission Electron Microscopy Study of the Neonatal Rat Tongue Mucosa Treated with Special Attention to the Bacteriae on the Epithelial Cell Membrane International Journal of Morphology
Picoli,Lara Cristina; Lopes,Ruberval A; Semprini,Marisa; Sala,Miguel Angel; Ogawa,Koichi; Watanabe,Ii-sei.
The epithelial cell surfaces of neonatal Wistar rat tongue mucosa were examined by transmission electron microscopic method. The tissues were fixed by modified Karnovksy solution and embedded in Spurr resin. Thin sections of 90 nm thickness were cut and examined in the Jeol 1010 transmission electron microscopy. It was revealed that the queratinized squamous epithelial cells of the tongue mucosa exposed to cadmium presented groupings of numerous bacteriae which are attached to the flat cell membrane surfaces. The coccus and bacillus are attached to the cell membrane in organized rows or at random, as clearly seen in the TEM images. At high magnification, the transmission electron microscopic images showed that bacteriae are attached by a fibrillar...
Tipo: Journal article Palavras-chave: Epithelial cell; Tongue mucosa; Bacteria; Cadmium; Transmission electron microscopy.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300006
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Anatomical, Clinical and Radiographic Characteristics of Styloid Syndrome (Eagle Syndrome): a Case Report International Journal of Morphology
Buchaim,Rogério Leone; Buchaim,Daniela Vieira; Shinohara,André Luis; Rodrigues,Antonio de Castro; Andreo,Jesus Carlos; Ahmed,Farooque Jamaluddin.
Styloid syndrome is characterized by an elongated styloid process or calcification of the stylomandibular and stylohyoid ligament. This study describes a case of a 65-year-old woman who presented to the Stomatology Clinic, University of Marilia with temporomandibular joint pain, ear ringing and a reduced ability to open the mouth. Panoramic and posteroanterior digital radiographs showed bilateral elongation of the styloid processes, especially of the right side, whose length extended beyond the mandible angle. The diagnosis was confirmed with the association of clinical data and image examinations. The treatment options for styloid Syndrome include clinical follow-up, surgical removal of the styloid process or fracture of the elongated process. The case...
Tipo: Journal article Palavras-chave: Eagle syndrome; Elongated styloid process syndrome; Temporal bone.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200057
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Análisis Morfométrico de Hepatoesteatosis Microvesicular Alcohólica de Ratas International Journal of Morphology
Cornejo,R; Garrido,O; Sáez,L; Bustamante,C; Cartes,G.
Un total de 24 ratas hembras de 4 meses de vida con peso aproximado de 250 gramos fueron divididas en dos grupos de animales rotulados como A y B. El grupo A se mantuvo con pellet y agua ad libitum sirviendo como controles mientras que el grupo B conservaba el pellet y recibía una solución de alcohol 40% disuelto en agua lo cual derivó en una esteatosis alcohólica multivesicular. Ambos grupos se mantuvieron en estas condiciones por 60 días. Posteriormente las ratas fueron sacrificadas y se extrajeron muestras tanto de hígado normal-control como de hígado graso para enseguida ser procesadas para microscopía electrónica de transmisión. De hepatocitos normales y esteatósicos se obtuvieron microfotografías electrónicas de transmisión con aumentos finales de...
Tipo: Journal article Palavras-chave: Esteatosis; Alcohol; Morfometría; Hepatocito.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200019
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Age Effect in the Morphological Traits Performance for Sex Determination in Human Skulls and Mandibles International Journal of Morphology
Suazo Galdames,Iván; Zavando,Daniela.
In this study we tested the hypothesis that diagnostic performance of the morphological indicators for sexual dimorphism are reduced as they are applied in skull and mandibles of older subjects. We used 275 adult human skulls, 250 of these with mandible, all subjects with sex and age registry. Sixteen classic morphological indicators of sexual dimorphism were evaluated, this information was compared with the registry and results noted in terms of precision. The best general performance of morphological indicators of sexual dimorphism were recorded in the 31 - 40 and 61 - 70 years, age range groups. Lowest precision ws recorded in the group corresponding to subjects between 21 ­ 30 years. Our results do not support the proposed hypothesis and suggest a...
Tipo: Journal article Palavras-chave: Sexual dimorphism; Sex determination; Aging; Age; Skull.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100053
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Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells International Journal of Morphology
Bahmani,Tahere; Gholami,Samira; Mostafaie,Ali; Karimi,Ebrahim; Hoseinkhani,Zohreh; Azad,Taha; Mahdizadeh,Hamid; Mansouri,Kamran.
Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other...
Tipo: Journal article Palavras-chave: Olfactory mucosa; Neural stem cells; Hair cells; Differentiation; Rat.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200004
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Estereología Comparativa entre el Bazo del Cuye (Cavia porcellus) y la Rata (Rattus novergicus, Sprague Dawley) International Journal of Morphology
Furrianca,María Cristina; Vásquez,Bélgica; del Sol,Mariano.
El bazo es el órgano linfático periférico más grande del organismo y conocer sus aspectos morfológicos cuantitativos es importante para determinar posibles patologías. El objetivo del estudio fue determinar en dos especies: cuye (Cavia porcellus) y rata (Rattus novergicus Sprague Dawley), las características estereológicas del bazo, para obtener patrones de normalidad cuantitativos, los que servirán de base para futuros estudios morfofuncionales. Se utilizaron 5 bazos de cada especie, clínicamente sanos, obtenidos del Bioterio de la Universidad de La Frontera, Temuco, Chile. Los bazos fueron disecados y fijados en formalina tamponada al 10% y se determinó el volumen de éstos por el método de Scherle. Se obtuvieron 5 trozos por medio de Orientador los que...
Tipo: Journal article Palavras-chave: Estereología; Bazo; Cuye; Rata.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300003
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Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial International Journal of Morphology
Cabrera,Paulina; Sánchez,Raúl; Risopatrón,Jennie.
Los procedimientos de criopreservación inducen cambios morfofuncionales en los espermatozoides. Es importante post descongelación espermática utilizar procedimientos de selección que permitan recuperar espermatozoides altamente funcionales. El objetivo del presente estudio fue comparar la eficiencia del Swim-up y Equipure® en la selección de espermatozoides funcionales en semen descongelado de equino. Semen de 4 potros reproductores Criollos Chilenos (A, B, C y D), fueron descongelados separadamente y procesados (n=15) por: I.- Swim-up (SU) y II.- Equipure® (EQ). Post descongelación se determinó por citometría de flujo la viabilidad e integridad de membrana plasmática (SYBR-14/PI), potencial de membrana mitocondrial (YDm; JC-1), integridad de la membrana...
Tipo: Journal article Palavras-chave: Equino; Espermatozoide; Swim-up; Equipure®.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200057
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Quiste Broncogénico de Localización Poco Habitual: Reporte de 2 Casos y Revisión de la Literatura International Journal of Morphology
Bellolio J,Enrique; Tapia,Oscar; Guzman,Pablo; Villaseca H,Miguel.
El quiste broncogénico es un remanente del desarrollo de traquea y bronquios. Alteraciones en la migración de células durante el desarrollo, permite que estos grupos originen quistes revestidos por epitelio respiratorio. La ubicación más habitual es la intratoráxica, dentro de ella, la intrapulmonar es la más frecuente, seguida de la mediastínica. El diagnóstico definitivo se establece mediante la biopsia diferida. Es una patología poco frecuente y las presentaciones extra torácicas son muy infrecuentes, por lo que compartimos dos casos, uno de ubicación sublingual y otro de ubicación subcutánea en zona escapular.
Tipo: Journal article Palavras-chave: Quiste broncogénico; Quiste bronquial; Quiste congénito.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400040
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Histopathologicaltudies on the Effects of the Ethanolic Extract of the fruits of Garcinia kola on Selected Organs of the Dog International Journal of Morphology
Nottidge,H. O; Omobowale,T. O; Taiwo,V. O; Omotoso,M. A.
The ethanolic extract of Garcinia kola was administered to dogs to investígate the possible effects on selected organs of the dog. Two doses of the extract (500mg/Kg and lOOOmg/Kg) were daily administered to the test animals for a period of 6 weeks. A dose related response was observed in the severity of histopathological changes observed in the testes, liver, kidney and small intestine of animals in the test groups. Despite the reported potentially beneficial effects of Garcinia kola, its use as a medicinal plant should be with great caution.
Tipo: Journal article Palavras-chave: Garcinia kola; Dog; Histopathological effects.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400044
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The Human Body Known Through the Renovated Museum of Anatomy From the Faculty of Dentistry of Ribeirão Preto International Journal of Morphology
Rodrigues,Giovani Antônio; Ferreira,Pedro Franco; Morando,Bianca Carrilho; Issa,João Paulo Mardegan; Iyomasa,Mamie Mizusaki.
The present work aimed to preserve anatomical specimens to improve the quality of education, prepare supporting materials, and present lectures on the anatomy of the nervous system for public school students. Anatomical specimens related to the nervous system were photographed and named, and acrylic containers filled with formaldehyde were made for the accommodation of these specimens. After research on the subject a handbook and a banner were prepared, enriching the collections of the museum along with the anatomical specimens, in addition to the lecture and video available on "iptv.usp.br ". Meetings between the students, the teachers involved, and the members of the Secretariat of Education of Ribeirao Preto defined schools, dates, times, and duration...
Tipo: Journal article Palavras-chave: Neuroanatomy; Museum; Learning; Culture and extension.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300043
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Células Neuroendocrinas en Yemas Gustativas Primordiales International Journal of Morphology
Fierro-Murga,Ricardo; Ceballos-Salobreña,Alejandro; Gaitán-Cepeda,Luis.
El objetivo del trabajo fue identificar la presencia de células neuroendócrinas en yemas gustativas primordiales (fetales) humanas. Fueron utilizadas 15 lenguas fetales humanas obtenidas de abortos espontáneos (tiempo de gestación 23 semanas) de pacientes ingresados en el Hospital General, Chihuahua, México. Una muestra representativa del ápex lingual fue embebida en parafina y cortada a 3 micras para ser procesadas con la técnica inmunohitoquímica utilizando los siguientes anticuerpos: Anti-sinaptofisina; anti-proteína neurofilamento; anti-cromogranina; anti-citoqueratina 20; y anti proteínas-S100. El protocolo de investigación fue aprobado por los comités de bioética de las instituciones participantes. Se obtuvo la autorización de los padres para la...
Tipo: Journal article Palavras-chave: Yemas gustativas; Células gustativas; Neuroendócrino; Cromogranina.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200013
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Correlación entre Índice de Masa Corporal y Circunferencia de Cintura en una Muestra de Niños, Adolescentes y Adultos con Discapacidad de Temuco, Chile International Journal of Morphology
Ojeda Nahuelcura,Rodrigo; Cresp Barría,Mauricio.
La obesidad es uno de los problemas de salud pública más importantes, por lo que su detección en forma temprana facilita la aplicación de estrategias preventivas. Según la Organización Mundial de la Salud, el Índice de masa corporal (IMC), es un instrumento válido para determinar el estado nutricional de la población, afirmación no compartida por especialistas del alto rendimiento. Se realiza este estudio para correlacionar el IMC con la circunferencia de cintura (CC), indicador que permite predecir con mayor exactitud la obesidad central o abdominal. El segundo objetivo de esta investigación es describir la prevalencia del sobrepeso y la obesidad en personas con discapacidad. Se evaluó a 188 niños, adolescentes y adultos jóvenes de ambos sexos, 123...
Tipo: Journal article Palavras-chave: Índice de masa corporal; Circunferencia de cintura; Obesidad; Sobrepeso; Personas con discapacidad; Niños; Adolescentes; Adultos jóvenes.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400043
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Tejido Tiroideo y Láser Infrarrojo: Un Estudio Morfométrico International Journal of Morphology
Cornejo,Ricardo; Jaramillo,Roberto; Garrido,Orlando; Vergara,Luis.
La glándula tiroides posee gran importancia debido a la síntesis y secreción de hormonas, las cuales desempeñan funciones fundamentales para la mantención de la fisiología animal. En este contexto, el objetivo del presente trabajo consistió en determinar parámetros morfométricos de estructuras tiroideas sometidas a estimulaciones con láser infrarrojo (LIR). Para ello, 10 ratas Sprague Dawley de 3 meses de vida y peso aproximado de 200 g, fueron divididas en dos grupos de 5 animales cada uno: grupo control y grupo experimental. Estos últimos recibieron estimulaciones infrarrojas en la tiroides con dosis de 16 J/cm2 durante 15 días seguidos. Una vez sacrificadas las ratas, se extrajeron las glándulas tiroides las que fueron procesadas para microscopía óptica...
Tipo: Journal article Palavras-chave: Tiroides; Morfometría; Láser infrarrojo.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300044
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Academic Achievement and Perception of Two Teaching Methods in Histology: Light Microscopy and Digital Systems International Journal of Morphology
Becerra G,Daniela; Grob L. P,Melisa; Rodríguez R,Ángel; Barker M,María José; Consiglieri L,Lucas; Ferri G,Giorgio; Sabag S,Natividad.
With the advent of digital systems, the role of the microscope as an irreplaceable instrument in the practical teaching of histology has been called into question. In this study academic performance and student perception for three learning methods was compared: digital systems, microscopy, and microscopy plus digital systems, in the muscle tissue unit of the morphology course for first-year dentistry at the Universidad de los Andes, Santiago, Chile. Ninety-five students were divided into 3 groups: Group 1: individual optical microscopy, Group 2: digital systems (one projector per room), and Group 3: microscopy plus digital systems. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle mounts. Their diagnostic...
Tipo: Journal article Palavras-chave: Medical education; Histology; Visual learning; Virtual slides; Microscopy.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300001
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Depurando la Terminologia Anatomica: Apertura y Foramen, Dos Similitudes y Redundancia Terminológica International Journal of Morphology
Duque Parra,Jorge Eduardo; Barco Rios,John; Duque Quintero,Natalia.
El uso redundante de términos en el campo de la morfología, además de dificultar el aprendizaje de los estudiantes, va en contra de los preceptos establecidos por el Programa Federativo Internacional para la Terminología Anatómica (FIPAT) sobre el uso de una terminología anatómica unificada, mediante la cual se pretende evitar la redundancia terminológica. Se propone suprimir el término apertura piriforme y reemplazarlo por el término foramen piriforme, con el fin de evitar la ambigüedad y la utilización de distintos nombres que describen a una estructura de similares características.
Tipo: Journal article Palavras-chave: Apertura; Foramen; Terminología.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400037
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Teratogenic Effect of Ethylene Glycol-Methyl Cellosolve Mixture in Rats.: I. Reproductive Damage International Journal of Morphology
Arteaga-Martínez,Manuel; García-Peláez,Isabel; Aguirre-Luna,Oswaldo M; Saavedra-Ontiveros,Dolores.
We investigated the reproductive damage and teratogenic effect of an ethylene glycol-methyl cellosolve mixture on gestating Wistar rats, which received a daily intraperitoneal dose of different concentration of the mixture on day 8 of gestation until day 20. In rats treated with the mixture the number of live fetuses decreased and reabsorptions increased with increasing concentrations of the mixture, as well as the number of abnormal fetuses. We conclude that the ethylene glycol-methyl cellosolve mixture possesses a higher teratogenic potential than each of its constituents separately, producing reproductive damage, external fetal abnormalities, growth delay, and increased fetal death.
Tipo: Journal article Palavras-chave: Ethylene glycol; Glycol ethers; Methyl cellosolve; Rat fetuses; Teratogenesis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400016
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Variations in The Vascular Supply of Pancreas and Transverse Colon: A Case Report International Journal of Morphology
Silva Júnior,L. M; Alexandre,M; Gonçalves,L; Silva,F. S; Caetano,A. G.
During anatomical dissection of the abdominal cavity of an adult male corpse, a changing pattern of vascular supply of pancreas and of the transverse colon was observed. The inferior pancreatic artery and the middle colic artery arose from a common trunk of the superior mesenteric artery. Moreover, the lienal artery did not direct a dorsal branch to the neck of the pancreas, but rather sent two greater pancreatic arteries (proximal and distal) to the body of the gland and one caudal artery to the terminal portion of the pancreas. We also observed a large communicating branch between the proximal portion of the middle colic artery and the ascending branch of the left colic artery, previously described as artery of Moskowitz. Based on these data, clinical...
Tipo: Journal article Palavras-chave: Anatomic variation; Blood supply; Pancreas; Large intestine.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100032
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Pulpa Dentaria Sana vs. Pulpitis Reversible: Caracterización Estereológica de Fibroblastos International Journal of Morphology
Trujillo,Erick; Morales,Rolando; Roa,Ignacio.
La reacción y reparación de la dentina depende del número de células presentes en la pulpa, dentro de éstas fibroblastos. Los métodos diseñados para obtener una estimación fiable de la cantidad de elementos celulares de la pulpa han sido subjetivos y sesgados, sobre todo al evaluar los cambios cuantitativos y potencial capacidad reparadora en presencia de caries. El objetivo fue estimar y comparar cuantitativamente las densidades de número, volumen y superficie de fibroblastos en pulpas sanas y con diagnóstico de pulpitis reversible producto de caries en dientes humanos jóvenes. Se utilizaron dientes premolares humanos obtenidos de exodoncias, divididos en un grupo sano y cariado, los cuales fueron fijados y posteriormente descalcificados con ácido nítrico...
Tipo: Journal article Palavras-chave: Estereología; Pulpa dentaria; Fibroblastos.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300021
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Homoplasies, Consistency Index and the Complexity of Morphological Evolution: Catfishes as a Case Study for General Discussions on Phylogeny and Macroevolution International Journal of Morphology
Diogo,Rui.
Catfishes constitute a highly diversified, cosmopolitan group that represents about one third of all freshwater fishes and is one of the most diverse Vertebrate taxa. The detailed study of the Siluriformes can, thus, provide useful data, and illustrative examples, for broader discussions on general phylogeny and macroevolution. In this short note I briefly expose how the study of this remarkably diverse group of fishes reveals an example of highly homoplasic, complex 'mosaic' morphological evolution
Tipo: Journal article Palavras-chave: Catfishes; Homoplasies; Morphological macroevolution; Phylogeny; Siluriformes; Teleostei.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400024
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Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice International Journal of Morphology
Rengifo,Aura Caterine; Umbarila,Vanessa Jazmin; Garzón,Mary Janeth; Torres-Fernández,Orlando.
Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. Rabies-infected mice were sacrificed at the terminal stage of the disease. Control mice were also euthanized at the same age....
Tipo: Journal article Palavras-chave: Rabies; NeuN; Cerebral cortex; Mouse; Immunohistochemistry; Neurons.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031
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