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Experimental envenomation with Crotalus durissus terrificus venom in dogs treated with antiophidic serum - part II: laboratory aspects, electrocardiogram and histopathology J. Venom. Anim. Toxins incl. Trop. Dis.
Nogueira,R. M. B.; Sakate,M.; Sangiorgio,F.; Laposy,C. B.; Tostes,R. A..
The present work shows laboratory aspects, electrocardiogram and histopathology results during experimental envenomation by Crotalus durissus terrificus in dogs treated with antiophidic serum. Twenty-one dogs were divided into three groups of seven animals each. Group I received 1mg/kg venom (sc); Group II received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9% NaCl solution (iv); and Group III received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9% NaCl solution containing sodium bicarbonate diluted to the dose of 4mEq/kg. Urinalysis showed brown urine, proteinuria, occult blood and myoglobinuria. Respiratory acidosis and hypotension were also observed. At the venom inoculation site, there...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalus durissus terrificus; Dog; Laboratory aspects; Histopathology.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400011
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Management of severe scorpion sting at rural settings: what is the role of scorpion antivenom? J. Venom. Anim. Toxins incl. Trop. Dis.
Bawaskar,H. S..
Tipo: Info:eu-repo/semantics/other
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100002
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Environmental risk factors for canine toxoplasmosis in a deprived district of Botucatu, SP, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Camossi,LG; Faccioli,PY; Menozzi,BD; Daher,SR; Langoni,H.
Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii that can infect a large variety of animals, including humans. The present study aimed to evaluate the frequency of anti-T.gondii antibodies in dogs from a peripheral district of Botucatu and to establish the association among some epidemiological variables in order to evaluate risk factors for toxoplasmosis infection. Serum samples from dogs were screened using an indirect fluorescent antibody (IFA) test. Anti-T.gondii antibody prevalence was 56%. The highest titer was 1024 (1.79%) and the most frequent titers were 16 (57.14%) and 64 (33.93%). The chi-square (X²) test revealed significant association among variables such as dog access to street, ingestion of raw meat and presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Dogs; IFA; Risk factors.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300006
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The importance of standardization in the quality of a scientific publication J. Venom. Anim. Toxins incl. Trop. Dis.
Fujita,Mariângela Spotti Lopes.
Tipo: Info:eu-repo/semantics/other
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300001
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Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system J. Venom. Anim. Toxins incl. Trop. Dis.
Lopes-Ferreira,Monica; Grund,Lidiane Zito; Lima,Carla.
Thalassophryne nattereri (niquim) is a venomous fish found off North and Northeast coast of Brazil, where it is known by the severity of the accidents involving humans. This review article is divided into four topics. The first one provides a brief description of the animal biology and its distribution off Brazilian coastal waters, the venom apparatus, signs and symptoms observed in envenomated humans and also describes envenomation in mice. The second topic describes the use of modern genetic approach and mass spectrometry for identification of highly expressed genes in its venom glands and the sequence of major toxins. The third chapter offers a detailed study of tissue injury induced by the venom and reveals the role of toxins that impair inflammation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Venomous fish; Thalassophryne nattereri; Immunological memory; Nattectin; Natterin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200208
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Clinical and cardiovascular alterations produced by scorpion envenomation in dogs J. Venom. Anim. Toxins incl. Trop. Dis.
Cordeiro,F. F.; Sakate,M.; Fernandes,V.; Cuyumjian,P. R..
Scorpionism is a common problem that occurs in tropical and subtropical countries and assumes great medical-sanitary importance due to its fatal effect on sensitive individuals, being able to lead children and aged people to death. The envenomation lethal potential is responsible for the serious cardiopulmonary alterations the scorpion toxin produces in its victims. The present research evaluated the effects of Tityus serrulatus venom on dogs, using two distinct doses: a dose that simulates natural envenomation (0.4 mg/total dose), and an experimental dose (0.25 mg/kg). General clinical signs were observed at different moments after envenomation, and specific data related to the cardiopulmonary system were evaluated by systemic arterial pressure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion; Dogs; Cardiopulmonary alterations; Tityus serrulatus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100003
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Clinical and biochemical manifestation produced by scorpion (Hemiscorpius lepturus) venom in experimental animals J. Venom. Anim. Toxins incl. Trop. Dis.
Mirakabbadi A.,Zare; Zolfagharian,H.; Hedayat,A.; Jalali,A..
Several studies have been published about the clinical and biochemical manifestations produced by the venom of scorpions of the Buthidae family, but very few reports have indicated the manifestations induced by the venom of the Scorpionidae family. Hemiscorpius lepturus is an important scorpion species present in the south and southwestern part of Iran, causing morbidity and mortality in children and adults. For the present study, H. lepturus venom was extracted by electric shock and subcutaneously injected (6.3mg/kg) into a group of six rabbits. Blood collection was carried out before and three hours after venom injection for determination of osmotic fragility and levels of blood sugar, alanine aminotransferase (ALT), aspartate aminotransferase (AST),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemiscorpius lepturus; Scorpions; Osmotic fragility; Iran; Biochemical changes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400007
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Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction J. Venom. Anim. Toxins incl. Trop. Dis.
CJ,Fecchio; LA,Dias-Melicio; AMVC,Soares; S,Guimarães; MTS,Peraçoli; A,Sartori.
Cell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate cellular immunity by the footpad test, in experimental murine paracoccidioidomycosis. Male mice infected with P. brasiliensis 265 strain were challenged in the footpad with CFAg obtained from four- (4d CFAg) or 11-day-old cultures (11d CFAg). The increase in footpad swelling provoked by 4d CFAg and 11d CFAg was similar and showed significant difference in relation to control groups. However, the infiltrate pattern was strikingly different: 4d CFAg induced a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioides brasiliensis; Cell-free antigens; Delayed-type hypersensitivity reaction; Gp43; Murine paracoccidioidomycosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300012
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Hymenoptera marking technique J. Venom. Anim. Toxins incl. Trop. Dis.
Pereira,A. M.; Chaud-Netto,J..
In true social hymenopterans, such as many species of bees, wasps and all species of ants, the main characteristics are the overlapping of generations, the care with the offspring and the division of labor among the members of the colony. The first biological feature means that in a same moment there are groups of individuals, with variable ages, that execute different activities in the colony. In order to study the division of labor among the members of the colony, or to estimate the life span of these insects, or even to analyze any kind of behavior in non-social insects, it is necessary to know the exact age of each individual. For this reason, the insects must be identified soon after emergence. The identification of insects with numbers is an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Hymenoptera; Marking technique; Social insects.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100014
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Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India J. Venom. Anim. Toxins incl. Trop. Dis.
Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A..
Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008
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Decrease in hepatitis B prevalence among blood donors in Central-West Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Lindenberg,Andréa de Siqueira Campos; Motta-Castro,Ana Rita Coimbra; Puga,Marco Antonio; Ortiz Tanaka,Tayana Serpa; Torres,Marina Sawada; Fernandes-Fitts,Sonia Maria; Cunha,Rivaldo Venancio.
Background The aim of the present study was to estimate hepatitis B virus seroprevalence among first-time blood donors in the city of Campo Grande, Mato Grosso do Sul State, in the central-western region of Brazil. Findings A retrospective analysis of first-time voluntary blood donor records, from January 2010 to December 2010, was conducted at the Hematology Center of Mato Grosso do Sul. The prevalence of the HBsAg and anti-HBc serological markers and their respective 95% confidence intervals were calculated. Chi-square analysis was performed between the seroprevalence previously found in 2001 and the one determined by the current study. Results were considered statistically significant if p < 0.05. Among 8,840 subjects, 269 (3.04%, 95% CI: 2.7-3.4)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hepatitis B virus; Prevalence; Blood donors.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100402
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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice J. Venom. Anim. Toxins incl. Trop. Dis.
Machado,JG; Hoffmann,JL; Krause,VLK; da Silva,AV; Dias-Melicio,LA; Langoni,H.
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Immunosuppression; Immunopathology; Mice; Interleukins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013
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Retrospective seroepidemiological analysis of patients with suspicion of paracoccidioidomycosis in São Paulo State, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Kamikawa,CM; Kohara,VS; Passos,AN; Vicentini,AP.
The geographic distribution of paracoccidioidomycosis (PCM) in the Brazilian state of São Paulo was evaluated in a retrospective study using secondary data from serological analyses, carried out by double immunodiffusion assay of patients with PCM suspicion, from January 1999 to May 2010. Sixty percent of 10,176 patients, from 239 cities, were serologically reactive to P. brasiliensis. The cities that showed the most serological reactivity among patients were São João da Boa Vista (85%), Piracicaba (75%), Sorocaba (73%), Campinas (72%) and São Paulo (62%). São Paulo state has an area of 248,209.4 km²; the climate is tropical and sub-tropical with annual temperatures between 18 and 24ºC, high rainfall (900 to 1800 mm/year), rainy summers and mild winters....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioides brasiliensis; Paracoccidioidomycosis; Immunological tests; Seroepidemiological studies.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300003
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Paracoccidioidomycosis surveillance and control J. Venom. Anim. Toxins incl. Trop. Dis.
Mendes,RP.
Tipo: Info:eu-repo/semantics/other
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200003
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Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines J. Venom. Anim. Toxins incl. Trop. Dis.
Silva,Cássio Prinholato da; Costa,Tássia R.; Paiva,Raquel M. Alves; Cintra,Adélia C. O.; Menaldo,Danilo L.; Antunes,Lusânia M. Greggi; Sampaio,Suely V..
Abstract Background Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. Methods The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops jararacussu; BthTX-I; Antitumor potential; Apoptosis; Cell cycle alterations.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348
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The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels J. Venom. Anim. Toxins incl. Trop. Dis.
Badhe,R. V.; Thomas,A. B.; Deshpande,A. D.; Salvi,N.; Waghmare,A..
Red scorpion (Mesobuthus tamulus or Buthus tamulus) venom samples were collected at different regions of India: western (Chiplun and Ahmednagar from Maharashtra State) and southern (Ratnagiri and Chennai from Tamil Nadu State). The action of whole venoms on the blood sodium levels of mice was assessed using flame photometry. Seven peptides were common to all venom samples. They were separated using the native polyacrylamide gel electrophoresis (PAGE) technique and their activities were also studied using flame photometry. There was a decrease in the concentration of sodium ions in the serum, which suggested the blockage of such ions by scorpion venom toxins. Among the 10 protein bands isolated, the band at 79.6 kDa presented maximum activity in decreasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red scorpion; Buthus tamulus; Venom activity; Sodium ion concentration; Venom protein fraction; Pharmacological action.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100006
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Evaluation of intestinal bacterial flora and HIV-1 infected individual's nutritional status, supplemented with soluble fiber and probiotic J. Venom. Anim. Toxins incl. Trop. Dis.
Trindade,E. B. S. M..
Tipo: Info:eu-repo/semantics/other
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300015
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Epidemiological characterization and prognostic factors in patients with confirmed cerebral cryptococcosis in central Taiwan J. Venom. Anim. Toxins incl. Trop. Dis.
Chen,Chang-Hua; Sy,Hiu-Ngar; Lin,Li-Jhen; Yen,Hua-Cheg; Wang,Shao-Hung; Chen,Wei-Liang; Chen,Yu-Min; Chang,Yu-Jun.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcus neoformans; Cryptococcus gattii Cryptococcosis; Meningitis; Outcome; Risk Assessment.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100320
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Effect of Curcuma zedoaria crude extract against tumor progression and immunomodulation J. Venom. Anim. Toxins incl. Trop. Dis.
Carvalho,FR; Vassão,RC; Nicoletti,MA; Maria,DA.
The aim of the present work was to study the effect of the crude extract of Curcuma zedoaria on peripheral blood cells and tumor progression in C57Bl/6J mice injected with B16F10 murine melanoma cells. The intraperitoneal therapy showed a significant increase in total white and red blood cell counts, a decrease in peritoneal cell number and tumor volume reduction, whereas the oral administration revealed a noteworthy augmentation only in total leukocyte count. These results contribute to evaluate the importance of alternative treatments that employ phytotherapic compounds against tumor progression and its possible immunomodulation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melanoma; Tumor; Hematology; Curcuma zedoaria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200013
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CEVAP - Present, past and future J. Venom. Anim. Toxins incl. Trop. Dis.
Barraviera,Silvia Regina C. Sartori.
Tipo: Info:eu-repo/semantics/report
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100001
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