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Registros recuperados: 94 | |
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Vieira-da-Motta,Olney; Eckhardt-de-Pontes,Luiz Antonio; Petrucci,Melissa Paes; Santos,Israel Pereira dos; Cunha,Isabel Candia Nunes da; Morato,Ronaldo Gonçalves. |
Both the study of Brazilian wild mammal fauna and the conditions that foster the preservation of endangered species, such as Brazilian Maned-wolf (Chrysocyon brachyurus), in wild life are of extreme importance. In order to study the resistance profile of microbiota bacterial colonizing Brazilian Maned-wolf, this work investigated samples from eight male captive and free roaming animals originating from different Brazilian geographical regions. Samples for microbiological purposes were collected with swabs and kept in appropriate transport medium. Using routine microbiological techniques, the isolated bacteria were tested toward antimicrobial drugs by the agar disk diffusion method. Results showed that all samples from wild animals were sensitive toward all... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Maned-wolf; Chrysocyon brachyurus; Microbiota; Antimicrobial resistance; Anthropic pressure; Environment. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400042 |
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Kottwitz,Luciana B.M.; Leão,Joice Aparecida; Back,Alberto; Rodrigues,Dalia dos P.; Magnani,Marciane; Oliveira,Tereza C.R.M. de. |
Salmonella enterica is frequently associated with outbreaks of human salmonellosis, and products of avian origin, such as eggs and chicken meat, are the main vehicles of its transmission. The present study describes the occurrence of different serovars of Salmonella enterica and phagotypes of S. enterica serovar Enteritidis in eggs destined for human consumption. Four thousand eggs obtained from commercial egg laying farms and one thousand discarded hatching eggs from broiler farms, which were acquired at farmers' markets and informal shops, were analyzed. Salmonella spp. was isolated from 52.0% of the discarded hatching eggs, in which the predominant serovar was Enteritidis (84.6%), and the predominant Salmonella Enteritidis phagotype (PT) was PT7... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Discarded eggs; Salmonella Enteritidis; Antimicrobial resistance; Salmonellosis. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200004 |
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Oliveira,Sílvia Dias de; Bessa,Marjo Cadó; Santos,Luciana Ruschel dos; Cardoso,Marisa Ribeiro de Itapema; Brandelli,Adriano; Canal,Cláudio Wageck. |
In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultry-related samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Rep-PCR; Phage typing; Antimicrobial resistance; Virulence genes. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400025 |
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Cassenego,A.P.V.; d'Azevedo,P.A.; Ribeiro,A.M.L.; Frazzon,J.; Sand,S.T.Van Der; Frazzon,A. P. G.. |
Resistant bacteria in animal can be spread to environment and to humans. Poultry feed and infections caused by Eimeria spp. are important factors in determining the intestinal microbial communities. The aim of this study was to verify the prevalence of species and antimicrobial susceptibility of Enterococcus isolated from broilers fed with different supplements and infected experimentally with Eimeria spp. Broilers were divided in eight groups, fed with diets supplemented with a combination of antimicrobial, ionophore-coccidiostatics, probiotic, essential oil. At 14 days old all birds, except the control, received a solution containing oocysts of Eimeria spp. Samples of cloacal swabs from broilers were collected. A total of 240 Enterococcus sp. strains... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus sp.; Broilers feed; Antimicrobial resistance; Resistance genes. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200012 |
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Rahimi,Ebrahim. |
This study was conducted to determine the prevalence rate, enterotoxigenecity, and antimicrobial resistance of S. aureus isolated from dairy products in Iran. From September 2010 to July 2011, a total of 347 samples from various dairy products, traditional and commercial, were collected from randomly selected retail stores. Overall, 20 samples (5.8%) were found to be contaminated with S. aureus. The highest prevalence of S. aureus was found in traditional cheese (11.1%), followed by traditional ice-cream (5.9%), cream (5.6%), and butter (5.3%). The ability to synthesize classical staphylococcal enterotoxins (SEA-E) was determined in 7 of 20 (35%) isolates by using ELISA. SE type C was the most common enterotoxin found in the isolated S. aureus (42.9%),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Dairy products; SE genes; Staphylococcal enterotoxins; Staphylococcus aureus. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200008 |
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Guerra,Ivani M.F.; Fadanelli,Raquel; Figueiró,Manuela; Schreiner,Fernando; Delamare,Ana Paula L.; Wollheim,Claudia; Costa,Sérgio Olavo P.; Echeverrigaray,Sergio. |
Aeromonas were isolated from 27 (6.6%) of 408 patients admitted with acute gastroenteritis in two hospitals at Rio Grande do Sul, Brazil. Isolates were classified as A. hydrophila (51.8%), A. caviae (40.8%), and A. veronii biotype sobria (7.4%). The highest prevalence of Aeromonas associated infections occurred in lactants and children. Virulence genes (aerA -aerolysin/hemolysin, ahpA -serine-protease, satA - glycerophospholipid-cholesterol acyltransferase, lipA -lipase, and ahyB -elastase) and virulence factors (hemolytic, proteolitic, lipolitic activities, and biofilm formation) were identified in most A. hydrophila and A. veronii biotype sobria isolates, with lower frequencies on A. caviae. All Aeromonas isolates were resistant to ampicillin,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Diarrhea disease; Virulence factors; Antimicrobial resistance. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400011 |
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Melo,Dayanne Araújo de; Coelho,Irene da Silva; Motta,Cássia Couto da; Rojas,Anna Carolina Coelho Marín; Dubenczuk,Felipe Carlos; Coelho,Shana de Mattos de Oliveira; Souza,Miliane Moreira Soares de. |
Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA LGA251 in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA LGA251 gene. For mecA gene PCR, new primers were designed based on... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Bovine mastitis; Staphylococccus; MecA gene. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300041 |
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Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de. |
Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118 |
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Bada-Alambedji,Rianatou; Fofana,Aïssatou; Seydi,Malang; Akakpo,Ayayi Justin. |
This study was undertaken to estimate the antimicrobial resistance of Salmonella isolated from raw chicken. From November 2003 to April 2004 a total of 120 chicken carcasses were collected from 36 randomly selected sale points (supermarkets, traditional market, poultry slaughter house, flocks) in the urban and periurban zones of Dakar, Sénégal, and examined for the presence of Salmonella. Salmonella was isolated from 75 (62.5%) of the examined samples. Out of the 90 Salmonella isolates obtained, twenty one serotypes were identified, from which the most prevalent were S. Kentuchy 30%, S. Muenster (13.3%), S. Brancaster (8.8%), S. Enteritidis and S. Hadar (6.6%). All Salmonella isolates were tested for their susceptibility to 16 selected antimicrobial agents... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella sp; Poultry; Antimicrobial resistance. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400020 |
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Balsalobre,Livia Carminato; Dropa,Milena; Matté,Maria Helena. |
Multiple papers have been published regarding the bacterial resistance theme over the last years. A variety of information has reached general and scientific public, daily bringing up data on new resistant microorganisms, new drugs, outbreaks, epidemiological news, resistance gene dissemination, and the lack of information in a particular field has caught our attention: the public health department. Most of researchers, physicians and government employees interpret the public health field as a separate department, not linked to this antibiotic resistance era that we are living nowadays. In this paper we carefully tried to fill in the blanks between public health and the bacteria resistance issue, also considering historical, social, economical and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Public health; Pre-antibiotic era. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100001 |
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Carvalho,A.C.; Barbosa,A.V.; Arais,L.R.; Ribeiro,P.F.; Carneiro,V.C.; Cerqueira,A.M.F.. |
Abstract Antimicrobial resistance in Escherichia coli isolated from pet dogs can be considered a potential threat of infection for the human population. Our objective was to characterize the resistance pattern, extended spectrum beta-lactamase production and genetic relatedness of multiresistant E. coli strains isolated from dogs (n = 134), their owners (n = 134), and humans who claim to have no contact with dogs (n = 44, control), searching for sharing of strains. The strains were assessed for their genetic relatedness by phylogenetic grouping and pulsed-field gel electrophoresis. Multiresistant E. coli strains were isolated from 42 (31.3%) fecal samples from pairs of dogs and owners, totaling 84 isolates, and from 19 (43.1%) control group subjects. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antimicrobial resistance; Dogs; Genetic relatedness. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100150 |
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Calazans-Silva,Amanda C.; Medeiros,Pedro T.C.; Araujo,Dayanne M.; Carvalho,Bruno O.; Coelho,Irene S.; Coelho,Shana M.O.; Souza,Miliane M.S.. |
Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus sciuri; MecA; PbpD; Antimicrobial resistance. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200038 |
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Duarte,Dalila Angélica Moliterno; Ribeiro,Aldemir Reginato; Vasconcelos,Ana Mércia Mendes; Santos,Sylnei Barros; Silva,Juliana Vital Domingos; Andrade,Patrícia Lúcia Arruda de; Falcão,Lúcia Sadae Pereira da Costa de Arruda. |
The present study was carried out to evaluate the occurrence of Salmonellae in broiler chicken carcasses and to determine the antimicrobial resistance profile of the isolated strains. Twenty-five out of the 260 broiler chicken carcasses samples (9.6%) were positive for Salmonella. S. Enteritidis was the most frequent serovar. Nineteen Salmonella isolates were tested for antimicrobial resistance, and the results indicated that 94.7% were resistant to at least one antimicrobial agent. Resistance to streptomycin (73.7%), nitrofurantoin (52.3%), tetracycline (31.6%), and nalidixic acid (21%) were the prevalent amongst Salmonella strains tested. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Broiler chicken carcasses; Antimicrobial resistance; Salmonella. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300020 |
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Pontes,Patricia Silveira de; Coutinho,Selene Dall’ Acqua; Iovine,Renata de Oliveira; Cunha,Marcos Paulo Vieira; Knöbl,Terezinha; Carvalho,Vania Maria de. |
Abstract We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM, blaSHV, blaOXA, blaCMY, blaCTX-M, tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)′-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups. Salmonella spp. was not detected. E. coli was isolated in 10% of the animals (9/94). Four APEC genes (ironN, ompT, iss and hlyF) were detected in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Salmonella sp; Cockatiel; Nymphicus hollandicus; Antimicrobial resistance. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500076 |
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Kuana,Suzete Lora; Santos,Luciana Ruschel dos; Rodrigues,Laura Beatriz; Borsoi,Anderlise; Moraes,Hamilton Luis do Souza; Salle,Carlos Tadeu Pippi; Nascimento,Vladimir Pinheiro do. |
The aim of this study was to assess the antimicrobial susceptibility of 62 Campylobacter spp. strains obtained from broiler flocks using the agar diffusion method. The Campylobacter spp strains were isolated from 22 flocks aged between 3 and 5 weeks of life, isolated from cloacae swabs, stools and cecal droppings in the farm and from the carcass rinsing in the slaughterhouse. Campylobacter spp strains were tested on Mueller-Hilton (MH) agar (27 samples) and MH plus TTC agar (35 samples). The antimicrobial susceptibility test revealed a 62.5% resistance to at least one drug, especially to enrofloxacin (71%), neomycin (50%), lincomycin (50%), tetracycline (43%), penicillin (42%), ceftiofur (33%) amoxicillin (27%), spiramycin (20%), ampicillin (18%) and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Broiler flocks. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400026 |
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Magalhães,Ana Paula Gomes de Oliveira; Pinto,Artur da Silveira. |
Thirty one Streptococcus pneumoniae invasive strains were isolated from a pediatric population in Belo Horizonte from June, 1999 to May, 2001. Penicillin, trimethoprim-sulfamethoxazole, tetracycline and chloramphenicol resistance rates for the isolates were 41.9, 58.1, 25.8 and 3.2%, respectively. Intermediate penicillin resistant (MICs between 0.1 and 1.0 µg/ml) and resistant (MICs > 2.0 µg/ml) isolates occured at rates of 38.7 and 3.2%, respectively. Resistance to erythromycin, ofloxacin, rifampin or vancomicyn was not detected. Ten S. pneumoniae serotypes (14, 5, 10 A, 6B, 15B, 18C, 6 A, 18 A, 19 A and 19 F) were identified. Serotype 14 (12 out of 31) was predominant among the isolates. Penicillin and trimethoprim-sulfamethoxazole resistance was more... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotyping. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300005 |
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Registros recuperados: 94 | |
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