SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Moura,Raniere M.; Melo,Arthur A.; Carneiro,Rômulo F.; Rodrigues,Cícera R.f.; Delatorre,Plínio; Nascimento,Kyria S.; Saker-Sampaio,Silvana; Nagano,Celso S.; Cavada,Benildo S.; Sampaio,Alexandre H..

Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but...

Essential oil of Mitracarpus frigidus as a potent source of bioactive compounds

Palavras-chave: Purification; Hemolysis; Cytotoxicity; Galactose.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973

Fabri,Rodrigo L.; Coimbra,Elaine S.; Almeida,Ana C.; Siqueira,Ezequias P.; Alves,Tânia M.A.; Zani,Carlos L.; Scio,Elita.

In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 µg/mL). On the other hand, it showed a strong...

Assessment of Duguetia furfuracea genotoxic and cytotoxic activity in bacteria and mice

Palavras-chave: Mitracarpus frigidus; Essential oil; Antimicrobial; Antileishmanial; Cytotoxicity.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400021

Silva,Carolina R.; Vieira,Pabline M.; Santos,Suzana C.; Chen-Chen,Lee.

Duguetia furfuracea (St. Hil.) Benth & Hook f. (1862), popularly known as "sofre-do-rim-quem-quer" and "araticum-seco", is a shrub of the Annonaceae family that occurs in several regions of Brazil. In folk medicine the infusion of its leaves and twigs is used to treat rheumatism and renal colic, whereas the seed powder is mixed with water to treat pediculosis. Previous studies have described biological activities of this plant with cytotoxic, antitumoral, trypanocidal, leishmanicidal, antiplasmodial and antiprotozoal effects. In the present work, genotoxicity and cytotoxicity of Duguetia furfuracea lyophilized leaf extract were evaluated using the prophage λ induction test (SOS-Inductest) and mouse bone marrow micronucleus test. Our results showed...

Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)

Palavras-chave: Cytotoxicity; Duguetia furfuracea; Genotoxicity; Micronucleus test; SOS-Inductest.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100015

BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE.

ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7...

Evaluation of cytogenotoxicity, antioxidant and hypoglycemiant activities of isolate compounds from Mansoa hirsuta D.C. (Bignoniaceae)

Palavras-chave: Cytotoxicity; Gas Chromatography; Phoradendron microphyllum; Phoradendron mucronatum.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991

PEREIRA,JOQUEBEDE R.; QUEIROZ,RAPHAEL F.; SIQUEIRA,ERLÂNIA A. DE; BRASILEIRO-VIDAL,ANA CHRISTINA; SANT’ANA,ANTÔNIO E.G.; SILVA,DANIEL M.; AFFONSO,PAULO R.A. DE MELLO.

ABSTRACT Mansoa hirsuta (Bignoniaceae) is a native plant from caatinga in Brazilian semiarid. This plant has been locally used as antimicrobial and hypoglycemiant agents, but their action mechanisms and toxicity remain largely unknown. Therefore, we evaluated the composition and antioxidant, cytoprotective and hypoglycemiant effects of raw extract, fractions and compounds from leaves of M. hirsuta. The cytogenotoxic effects of ursolic and oleanolic acids, the main phytotherapic components of this plant, were assessed. The raw extract and fractions presented steroids, saponins, flavonols, flavanonols, flavanones, xanthones, phenols, tannins, anthocyanins, anthocyanidins and flavonoids. The ethyl acetate fraction inhibited efficiently the cascade of lipid...

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Palavras-chave: Antioxidant; Caatinga; Cytotoxicity; Hypoglycemiant; Phytochemistry..

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100317

EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN.

Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed...

Cytotoxic sesquiterpene lactones from the aerial parts of Inula aucheriana.

Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621

Gohari,Ahmad Reza; Mosaddegh,Mahmoud; Naghibi,Farzaneh; Eslami-Tehrani,Bahara; Pirani,Atefeh; Hamzeloo-Moghadam,Maryam; Read,Roger W..

Inula aucheriana DC is a member of the family Asteraceae which is known to produce cytotoxic secondary metabolites noted as sesquiterpene lactones. In the present study, sesquiterpene lactones inuchinenolide B, 6-deoxychamissonolide (stevin) and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10),11(13)-dien-12,8α-olide were isolated from I. aucheriana. Inuchinenolide B and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10),11(13)-dien-12,8α-olide were further evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay to demonstrate cytotoxic activity with IC50 values of (56.6, 19.0), (39.0, 11.8), and (55.7, 15.3) μg/mL against HepG-2, MCF-7 and A-549 cells, respectively. The cytotoxic activity of the two evaluated sesquiterpene lactones...

Antimicrobial, antioxidant and cytotoxicity potential of Manihot multifida (L.) Crantz (Euphorbiaceae)

Palavras-chave: Cytotoxicity; Inula aucheriana; MTT assay; Sesquiterpene lactones.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200777

FABRI,RODRIGO L.; SÁ,DANIEL S. DE; PEREIRA,ANA PATRÍCIA O.; SCIO,ELITA; PIMENTA,DANIEL S.; CHEDIER,LUCIANA M..

Manihot multifida (L.) Crantz (Euphorbiaceae) is widely used in popular medicine for the treatment of infected wounds. This study evaluated thein vitro antioxidant and antimicrobial potential of this species against strains of Gram-positive and Gram-negative bacteria and fungi, known to cause infections in humans. The extracts showed minimal inhibitory concentration (MIC) varying from 39 to 2500 µg/mL for antimicrobial activity. The methanolic extract of fruits, aqueous and hexane extracts of leaves showed a very strong activity against Candida albicans(ATCC 18804) with MIC of 39 µg/mL. Furthermore, the methanolic extract of M. multifida leaves exhibited DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging potential with inhibitory concentration (IC50) values...

Acute Toxicity and Cytotoxicity Effect of Ethanolic Extract of Spondias tuberosa Arruda Bark: Hematological, Biochemical and Histopathological Evaluation

Palavras-chave: Antimicrobial; Antioxidant; Candida albicans; Cytotoxicity; Manihot multifida.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100303

BARBOSA,HUMBERTO M.; NASCIMENTO,JAILSON N. DO; ARAÚJO,THIAGO A.S.; DUARTE,FILIPE S.; ALBUQUERQUE,ULYSSES P.; VIEIRA,JEYMESSON R.C.; SANTANA,EDSON R.B. DE; YARA,RICARDO; LIMA,CLÁUDIA S.A.; GOMES,DAYANE A.; LIRA,EDUARDO C..

ABSTRACT Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor...

Butein imparts free radical scavenging, anti-oxidative and pro-apoptotic properties in the flower extracts of Butea monosperma

Palavras-chave: Acute toxicity; Cytotoxicity; Phytomedicine; Safety; Spondias tuberosa Arruda; Toxicology.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501993

Biocell

Sehrawat,Anuradha; Kumar,Vijay.

The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules. The levels of cell injury markers like lactate dehydrogenase, glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured. The...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200003

Palavras-chave: Butrin; Cytotoxicity; Hepatoprotection; Methanol extract; Free radical scavenging.

Ano: 2012

The cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of Azadirachta indica (A Juss.)

Carneiro,Sabrina M. P; Carvalho,Fernando A. A; Santana,Lorena C. L. R; Sousa,Alessian P. L; Neto,José M. M; Chaves,Mariana H.

The leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus Leishmania. Studies with medicinal plants can lead to a range of possibilities for treating and improving the patients' quality of life. Research on Azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. In this research the most efficient extracts and fractions were selected for tests on intracellular amastigotes of Leishmania amazonensis. The ethanolic extract of the leaves and dichloromethane and chloroform fractions had IC50 values of 38, 3.9 and 1.2 μg/mL for...

Tipo: Journal article

Palavras-chave: Anti-leishmanial; Azadirachta indica; Cytotoxicity; Leishmaniasis; Leishmania amazonensis.

Ano: 2012

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200002

Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus

Sadiq,Anwar Zeb Abdul; Ullah,Farhat; Ayaz,Sajjad Ahmad Muhammad.

BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and...

Tipo: Journal article

Palavras-chave: Isodon rugosus; Cytotoxicity; Phytotoxicity; Brine shrimps.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100051

Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line

Extraction of saponins and toxicological profile of Teucrium stocksianum boiss extracts collected from District Swat, Pakistan

Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R.

Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine...

Tipo: Journal article

Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR.

Ano: 2011

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005

Shah,Syed Muhammad Mukarram; Sadiq,Abdul; Shah,Syed Muhammad Hassan; Khan,Shahzeb.

BACKGROUND: The current era is facing challenges in the management of neoplasia and weeds control. The currently available anti-cancer and herbicidal drugs are associated with some serious side effects. Therefore numerous researchers are trying to discover and develop plant based alternative particularly for the rational management of cancer and weed control. Teucrium stocksianum possess antioxidant and analgesic activities. The current study was designed to evaluate crude saponins (CS), methanolic extract and sub-fractions of T. stocksianum for cytotoxic and phytotoxic potentials. CS, methanolic extract and sub-fractions were extracted from powdered plant material using different solvents. Cytotoxic potential of the extracts at a dose of 10, 100 and 1000...

Tipo: Journal article

Palavras-chave: Saponins; Teucrium stocksianum; Cytotoxicity; Phytotoxicity.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100059

Induction of programmed cell death by Prangos uloptera, a medicinal plant

ZAHRI,SABER; RAZAVI,SEYED MEHDI; NIRI,FARSHAD HASSANZADEH; MOHAMMADI,SARIEH.

Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic...

Tipo: Journal article

Palavras-chave: Apoptosis; Cytotoxicity; McCoy; Prangos uloptera.

Ano: 2009

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013

Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)

Identification of a fat-soluble fraction of Sparganium stoloniferum that inhibits cervical cancer HeLa cells

Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N..

BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (μg/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin...

Tipo: Journal article

Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057

Zhang,Junwu; Wei,Yahui.

ABSTRACT Context The rizoma of Sparganium stoloniferum has been used as a traditional Chinese medicinal herb for thousands of years. Sparganium stoloniferum is a stasis-breaking drug to treat a wide range of diseases including cancer,however, its activity of extract on cervical cancer HeLa cells and the mechanisms remains unknown. Objective This study aimed to screen Sparganium stoloniferum extract for its inhibitory effects on cervical cancer HeLa cells. Materials and methods Sparganium stoloniferum was extracted with 95% ethanol under reflux, and the extracts were preliminary separated by silica gel column chromatography. MTT assay and flow cytometry were used to determine the inhibitory effects of three fractions on HeLa cells. In addition, GC-MS was...

Cytotoxicity and genotoxicity of untreated hospital effluents

Palavras-chave: Sparganium stoloniferum; Fat-soluble active fraction; Cytotoxicity; HeLa cells.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100337

Ortolan,Maria da Graça Silva; Ayub,Marco Antônio Záchia.

Untreated hospital effluent samples were tested for cytotoxic and genotoxicity in order to access whether they presented any serious health hazards. Results of umuC test suggested that the effluent from a large, general hospital could be considered weakly genotoxic. Comparatively, effluents from inpatient units presented higher frequency of genotoxicity than those coming from laboratory facilities. Although no cytotoxic activity was detected in laboratory effluent samples, cytotoxicity appeared to be an important problem of effluents from inpatient units.

Potential Use of Polysaccharides from the Brown Alga Undaria pinnatifida as Anticoagulants

Palavras-chave: Genotoxicity; Cytotoxicity; Untreated hospital effluents; Water sources contamination.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400009

Faggio,Caterina; Morabito,Marina; Minicante,Simona Armeli; Piano,Giada Lo; Pagano,Maria; Genovese,Giuseppa.

Undaria pinnatifida (U. pinnatifida) is a highly invasive species and has caused concern all over the world because it has invaded coastal environments, has the potential to displace native species, significantly alters habitat for associated fauna, and disturbs navigation. Any attempt to eradicate it would be futile, owing to the elusive, microscopic gametophyte, and because the alga thrives in sites rich in anthropic activities. Venice Lagoon is the largest Mediterranean transitional environment and the spot of the highest introduction of non-indigenous species, including U. pinnatifida, which is removed as a waste. We demonstrated that polysaccharide extracts from U. pinnatifida have an anticoagulant effect on human blood in vitro and are not cytotoxic....

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Palavras-chave: Algal extract; Anticoagulant activity; Cytotoxicity; Fucoidans; Human blood cells; Undaria pinnatifida.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500798

Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.

Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (<100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p<0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...