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The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax ArchiMer
Bossus, Maryline; Charmantier, Guy; Blondeau-bidet, Eva; Valletta, Bianca; Boulo, Viviane; Lorin-nebel, Catherine.
Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW,...
Tipo: Text Palavras-chave: ClC-3 chloride channel; Na+/K+-ATPase; Dicentrarchus labrax; Osmoregulation; Osmosensing.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00149/26044/25185.pdf
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Regulation of feed intake, growth, nutrient and energy utilisation in European sea bass (Dicentrarchus labrax) fed high fat diets ArchiMer
Boujard, Thierry; Gelineau, Anne; Coves, Denis; Corraze, Genevieve; Dutto, Gilbert; Gasset, Eric; Kaushik, Sadasivam.
Three practical isoproteic (54% protein) diets were formulated to contain graded levels of crude fat (diet L: 10%, diet M: 20% and diet H: 30%). Each diet was assigned unrestrictedly to three and restrictedly to two replicate groups of fish (IBW 243 g). In unrestricted groups, increasing the dietary lipid level led to a significant decrease in voluntary feed intake without affecting growth rate. In the feed-restricted groups, daily growth rates increased with increasing dietary fat levels. There was a significant and inverse effect of the dietary fat content on whole body moisture and fat levels, with highest lipid (ca. 20%) and lowest moisture (ca. 58%) contents in sea bass fed diet containing the highest lipid level; muscle lipid concentration was...
Tipo: Text Palavras-chave: Phosphorus excretion; Feed intake; Lipid; Digestible energy; European sea bass; Dicentrarchus labrax.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-392.pdf
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Le système immunitaire du bar Dicentrarchus labrax. Immunoglobulines, cellules productrices d'immunoglobulines, ontogenèse applications à une pathologie virale néonatale ArchiMer
Breuil, Gilles.
The sea bass, Dicentrarchus labrax, has been subjected to intensive aquaculture on the Mediterranean cost for ten years. Marine fish hatcheries are key factors in the aquaculture field, and face different pathologies, especially one of viral origin (nodavirus) that could challenge their feasibility. Consequently, we have tried to provide some data on larvae and spawning stocks. Firstly, we have studied the immunoglobulins, components of the specific humoral immunity, and their role as antibodies. On adult basses, these immunoglobulins are tetrameric molecules with high molecular weight (850 kD), made of heavy (H) - 80 kD and light (L) - 26 kD chains. In the eggs, simplest forms such as monomeric or dimeric are brought to the fore; they are located under...
Tipo: Text Palavras-chave: Egg; Cytometry; Immunity system; Larvae; Immunoglobulin; Nodavirus; Dicentrarchus labrax; Hatchery; Ontogenese; Cytometrie; Immunoglobuline; Nodavirus; Oeuf; Larve; Nurserie; Dicentrarchus labrax.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/these-1265.pdf
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Incorporation of dietary fatty acid in European sea bass (Dicentrarchus labrax) - A methodological approach evidencing losses of highly unsaturated fatty acids ArchiMer
Robin, Jean; Skalli, A.
A quantitative approach is presented to evaluate fatty acid incorporation in fish. Fatty acid composition of European sea bass juvenile was studied during an experiment using 6 isoproteic (54%) and isolipidic (18%) diets containing 0.23, 0.56, 0.72, 0.86, 1.01 and 1.86% DM n-3 highly unsaturated fatty acids (n-3 HUFA). Whole body fatty acid compositions were studied at the beginning and after 52 and 81 days feeding, fatty acid profiles evolved during time under dietary influences. Incorporations of individual fatty acids into total lipids were calculated as increases in individual fatty acids as percentages of the increase in total fatty acids during growth, on a per animal basis. Relative incorporations (RI) so defined generated similar profiles for the...
Tipo: Text Palavras-chave: Dicentrarchus labrax; HUFA; Incorporation; Composition; Fatty acids.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2272.pdf
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In search for indirect criteria to improve feed utilization efficiency in sea bass (Dicentrarchus labrax) Part II: Heritability of weight loss during feed deprivation and weight gain during re-feeding periods ArchiMer
Grima, Laure; Chatain, Beatrice; Ruelle, Francois; Vergnet, Alain; Launay, Amandine; Mambrini, Muriel; Vandeputte, Marc.
Selective breeding to improve residual feed intake (RFI) in sea bass (Dicentrarchus labrax) is a major goal that would optimize economic gain while minimizing the environmental impact of production. Due to the difficulty in accurately measuring individual feed intake, no selective breeding program has yet been started. In a previous study, we identified a criterion phenotypically related to RFI variations: the loss of weight during feed deprivation, FD. Moreover, an additional composite criterion (CC) integrating both FD and weight gain during subsequent re-feeding (RF) was closely related to RFI, even though the relationship was only close to significance (P=0.06). The aim of the present study was to estimate the heritability of these two traits, in order...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Feed efficiency; Heritability; Genetic parameters; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11529/8213.pdf
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L'élevage larvaire intensif du loup Dicentrarchus labrax. Influence de la lumière, de la densité en proies et de la température sur l'aluminium, sur le transit digestif et sur les performances zootechniques ArchiMer
Ronzani Cerqueira, Vinicius.
Several sea bass-rearing experiments were performed from hatching trough metamorphosis. The rearing units employed were 500 1 polyester tanks, where flow rate and temperature of seawater, light and suitable food (rotifer and artemia) were controlled. Stock density ranged between 50 and 100 larvae per liter. Individuals held in tanks with internal black walls developed faster and had a greater survival rate than those in white tanks. This suggests that a better contrast between preys and background assists the larvae to see food organisms more clearly. The sea bass larvae search for food is based only in vision and not trought mechanoreceptors or chemoreceptors. It was very difficult to evaluate the light intensity (50-5000 lux) influence on feeding,...
Tipo: Text Palavras-chave: Food; Prey; Light; Zootechnical; Digestive transit; Temperature; Dicentrarchus labrax; Intensive rearing; Sea bass.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/these-1676.pdf
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Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater ArchiMer
Siddique, Mohammad A. Momin; Butts, Ian A. E.; Linhart, Otomar; Macias, Adrian D.; Fauvel, Christian.
Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of...
Tipo: Text Palavras-chave: Egg longevity; Overripe eggs; Reproductive ecology; Mediterranean sea bass; Dicentrarchus labrax.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00279/39000/37580.pdf
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Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs ArchiMer
Colleter, Julie.
Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00300/41138/40326.pdf
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Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. ArchiMer
Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert.
The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf
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Low temperature has opposite effects on sex determination in a marine fish at the larval/postlarval and juvenile stages ArchiMer
Vandeputte, Marc; Clota, Frederic; Sadoul, Bastien; Blanc, Marie-odile; Blondeau-bidet, Eva; Bégout, Marie-laure; Cousin, Xavier; Geffroy, Benjamin.
Temperature‐dependent sex determination (TSD) can be observed in multiple reptile and fish species. It is adaptive when varying environmental conditions advantage either males or females. A good knowledge of the thermosensitive period is key to understand how environmental changes may lead to changes in population sex ratio. Here, by manipulating temperature during development, we confirm that cold temperature (16°C) increases the proportion of fish that develop as females in European sea bass (Dicentrarchus labrax) until 56 days posthatching, but show that it has an opposite effect at later stages, with the proportion of males reaching ~90% after 230 days at 16°C. This is the first observation of opposite effects of temperature at different time periods...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Fish; Sex ratio; Temperature-dependent sex determination; Thermosensitive period; TSD.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/76965/78184.pdf
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Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus ArchiMer
Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf
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Teneur en ADN de la larve de Dicentrarchus labrax : évolution ontogénétique et effet de la privation de nourriture ArchiMer
Bergeron, Jean-pierre; Person, Jeanine.
Sea bass Dicentrarchus labrax larvae were hatched and reared for 34 days in the laboratory to test a recent biochemical index (DNA/C) of larval fish nutritional condition. Variations in DNA/C ratio values were monitored during the whole larval ontogenesis of fed individuals and the effect of starvation was assessed at three developmental stages. Three main periods in the relative DNA content variations were determined in close connection with growth patterns during larval ontogenesis.
Tipo: Text Palavras-chave: Dicentrarchus labrax; DNA/C; Nutritional condition; Starvation; Growth; Biochemical index.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00072/18300/15875.pdf
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Genotype by environment interactions for growth in European seabass (Dicentrarchus labrax) are large when growth rate rather than weight is considered ArchiMer
Dupont-nivet, Mathilde; Karahan-nomm, Bilge; Vergnet, Alain; Merdy, Olivier; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice; Vandeputte, Marc.
Two hundred fifty three full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank for 14 months until reaching mean weight of 35 g. then 7000 of them were individually tagged and weighed, and dispatched to four farms in different locations (France, Israel, Italy and Portugal) representing a wide variety of environmental conditions. Around mean weight of 400 g. 1177 to 1667 fish at each site were weighed. Daily growth coefficient (DGC) was calculated. Pedigrees were successfully redrawn for 99.2% of fish using microsatellite markers. Genetic correlations between sites were high for body weight (>0.80 in all cases but one, i.e., five cases over six), but only...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Seabass; Growth; Daily growth coefficient; Heritability; G x E interactions; Aquaculture.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12256/9227.pdf
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Caractérisation et connectivité des Habitats Ecologiques Essentiels des stades adulte et juvénile du bar européen ArchiMer
Dambrine, Chloe.
Essential Fish Habitats host, at least, one stage of their life cycle. As they can be distant, maintaining their connectivity is essential for the closure of fish life cycle and populations’ sustainability. This thesis focused on the connectivity between spawning areas and nurseries of European seabass. Its main spawning areas have been characterised using a non-linear geostatistical approach applied to highly resolved fishery data. A Bayesian spatio-temporal model showed that they are mainly explained by a stable spatio-temporal structure, rather than by hydro-climatic factors. A bioenergetics model (DEB) over the entire life cycle of seabass was developed and validated for the early life stages. It showed that larvae and juveniles are resistant to low...
Tipo: Text Palavras-chave: Frayères; Nourriceries; Dynamique spatio-temporelle; Dicentrarchus labrax; Spawning areas; Nurseries; Spatio-temporal dynamic; Dicentrarchus labrax.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00676/78811/81055.pdf
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Développement et utilisation des marqueurs génétiques hypervariables chez les espèces marines. Rapport de synthèse 1995-1998 ArchiMer
Bonhomme, Francois; Gerard, Andre.
L'objectif scientifique de l'Unité de Recherche Marine n0 16 est de partager les savoir faire conceptuels et méthodologiques des deux équipes, pour faire aboutir des programmes appliqués ou fondamentaux dans le domaine de la génétique des organismes marins. Ces programmes concernent plus particulièrement le développement et l'utilisation des marqueurs génétiques fournis par les techniques de biologie moléculaire dans les programmes suivants: Chez les huîtres des genres Ostrea et Crassostrea : Le modèle prioritaire de l'URM sera les huîtres Crassostrea et Ostrea. Pour des raisons historiques, les recherches sur Ostrea sont plus avancées et donneront des résultats plus rapidement. 1-Sélection assistée par marqueurs chez Ostrea edulis et Crassostrea gigas...
Tipo: Text Palavras-chave: Génétique; Marqueur génétique; Biologie moléculaire; Sélection génétique; Ressource génétique; Recrutement; Bivalvia; Crassostrea; Ostrea; Pisces; Dicentrarchus labrax; Génotypage.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00043/15459/12834.pdf
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Induction of ovulation and stimulation of spermation by LHRH or an LHRH analog associated or not with pimozide. Effects on some teleost fish. ArchiMer
Billard, R; Weil, C; Barnabe, G.
Effectiveness of LHRH and one of its analogs (LHRH-A = D-Ala super(6) ethylamide) on induction of ovulation and stimulation of spermiation is reviewed here. LHRH was also combined with pimozide, an anti-dopaminergic agent, to try to cancel dopamine inhibition of gonatotropin (GtH) release. LHRH-A alone (50 mu g/kg) did not initiate ovulation but only oocyte maturation. When pimozide (10 mg/kg) and LHRH-A (50 mu g/kg) were combined, gonadotropic hormone secretion was significantly increased, causing ovulation and stimulating spermiation. This combination advanced ovulation in trout. Trout egg quality was markedly altered by the pimozide treatment; it remains to be tested in carp. In sea bass, LHRH-A was much more effective than LHRH, and ovulation was...
Tipo: Text Palavras-chave: Salmonidae; Dicentrarchus labrax; Sparidae; Cyprinidae; Teleostei; Pisces; Inhibitors; Sex hormones; Sexual maturity; Spermatogenesis; Ovulation; Induced breeding; Sexual reproduction.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1218.pdf
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Effect of selection for fasting tolerance on feed intake, growth and feed efficiency in the European sea bass Dicentrarchus labrax ArchiMer
Daule, Sophie; Vandeputte, Marc; Vergnet, Alain; Guinand, Bruno; Grima, Laure; Chatain, Beatrice.
Feed efficiency is a major goal for aquaculture sustainability, and selecting fish to genetically enhance this trait would be highly valuable. However, no selective breeding program specifically targeted to feed efficiency exists for farmed fish, mostly because of the difficulty of measuring individual feed intake. However, a negative phenotypic correlation between feed efficiency and weight loss at fasting has been previously demonstrated in sea bass submitted to feed deprivation (FD). We mated sea bass parents selected for their high (FD+) or low (FD-) weight loss at fasting to produce FD+ and FD- progeny, which were reared in a single tank to avoid common environmental effects. At 8 months of age, 1200 of those fish were submitted to three alternating...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Feed efficiency; Fasting tolerance.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/29985/28483.pdf
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Nourdem Loire : bilan de la campagne 2017 ArchiMer
Le Goff, Ronan; Drogou, Mickael; Le Ru, Loic; Garren, Francois; Martin, Stephane; Le Roy, Didier; Bouche, Ludovic; Berthele, Olivier.
Rapport de la campagne d'échantillonnage réalisée au moyen du chalut GOV "Bargip" entre le 29 juin et le 6 juillet 2017 dans l'estuaire de la Loire afin d'y produire l'indice d'abondance 2017 en juvéniles de bar (et autres espèces piscicoles).
Tipo: Text Palavras-chave: Bar européen; Nourricerie de l’estuaire de la Loire; Échantillonnage au chalut; Indice d’abondance 2017; Dicentrarchus labrax; European Sea Bass; Nurserie of the Loire estuary; 2017 Abundance index; Dicentrarchux labrax; Trawling net samplings.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00414/52544/53458.pdf
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Impact of a plant-based diet on behavioural and physiological traits in sea bass (Dicentrarchus labrax) ArchiMer
Benhaim, David; Begout, Marie-laure; Pean, Samuel; Manca, Michael; Prunet, Patrick; Chatain, Beatrice.
Replacing aquaculture feeds based on fisheries-derived resources with plant-based diets could be a relevant strategy to improve the sustainability of aquaculture. Recent studies on sea bass have shown that the total and early replacement of marine products by plant products would have a moderate effect on fish growth and body lipid content. Whether a plant-based diet impacts behavioural and physiological traits possibly linked to fish welfare, is not known, however. Here, we studied the effect of a totally plant-based diet introduced at an early stage of sea bass development on self-feeding behaviour, learning ability in a T-maze and stress biomarkers. We first compared learning processes in self-feeding conditions, between naive fish fed a plant-based...
Tipo: Text Palavras-chave: Self-feeder; Plant-based diet; Learning; Exploratory behaviour; Cortisol; Dicentrarchus labrax.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26309/24381.pdf
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The Na+/K+/2Cl(-) cotransporter in the sea bass Dicentrarchus labrax during ontogeny: involvement in osmoregulation ArchiMer
Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G.
This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after...
Tipo: Text Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf
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