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| Registros recuperados: 76 | |
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| Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.. |
| Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011 |
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| Teixeira,Maria F. S.; Lima Filho,José L.; Durán,Nelson. |
| The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectinases; Aspergillus japonicus; Enzyme; Galacturonase; Pectinesterases. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009 |
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| Divakaran,Dibu; Chandran,Aswathi; Pratap Chandran,R. |
| Alpha amylase (α-1, 4-glucan-glucanhydrolase, EC 3.2.1.1), an extracellular enzyme, degrades α, 1-4 glucosidic linkages of starch and related substrates in an endo-fashion producing oligosaccharides including maltose, glucose and alpha limit dextrin (7). The present study deals with the production and comparative study of production of α-amylase from two strains of Bacillus licheniformis, MTCC 2617 and 2618, by using four different substrates, starch, rice, wheat and ragi powder as carbon source by submerged fermentation. The effect of varying pH and incubation temperature, activator, inhibitor, and substrate concentration was investigated on the activity of α-amylase produced by MTCC strain 2618. The results shows that the production of the α-amylase by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Bacillus licheniformis; Enzyme; Extracellular; Glucose. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400022 |
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| Bisht,Sandeep; Pandey,Piyush; Bhargava,Bhavya; Sharma,Shivesh; Kumar,Vivek; Sharma,Krishan D.. |
| The remediation of polluted sites has become a priority for society because of increase in quality of life standards and the awareness of environmental issues. Over the past few decades there has been avid interest in developing in situ strategies for remediation of environmental contaminants, because of the high economic cost of physicochemical strategies, the biological tools for remediation of these persistent pollutants is the better option. Major foci have been considered on persistent organic chemicals i.e.polyaromatic hydrocarbons (PAHs) due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity. Rhizoremediation, a specific type of phytoremediation that involves both plants and their associated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PAH; Bioremediation; Rhizoremediation; Enzyme; Plant microbe pair. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100007 |
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| Naves,Luciana de Paula; Corrêa,Angelita Duarte; Bertechini,Antônio Gilberto; Gomide,Elisangela Minati; Abreu,Celeste Maria Patto de. |
| Two distinct experiments were conducted simultaneously with phytases of Aspergillus oryzae and A. niger for determining enzyme activity in response to storage period (up to 180 days at room temperature - RT). In the first experiment, enzymes were stored as they were acquired (pure form) and the activity was measured periodically during 180 days of storage at RT. In the second experiment, the phytases were incorporated to a supplement containing vitamins, minerals, and amino acids; and then this supplement was stored at RT up to 180 days, so that every 30 days of storage was collected one aliquot from each replicate for determining enzymatic activity. In conclusion, the phytase activity is affected by storage duration. To ensure 80% of the initial activity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Aspergillus oryzae; Enzyme; Phytate; Phytic acid. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100029 |
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| Catalan,Aiane Aparecida da Silva; Krabbe,Everton Luis; Avila,Valdir Silveira de; Lopes,Letícia dos Santos; Nunes,Ana Paula; Morés,Marcos Antonio Zanella; Roll,Victor Fernando Büttow; Xavier,Eduardo Gonçalves. |
| ABSTRACT: In order to evaluate the efficiency of phytase in diets with low and high phytate phosphorus (PP) 0content, as a consequence of wheat bran inclusion, on the relative weight of organs, intestinal morphometry and performance, three hundred and eighty-four male Cobb500 broilers were housed in metabolic cages. Animals were assigned into four treatments in a 2x2 factorial scheme in a randomized block design with eight replicates of 12 birds each. From 11 days of age birds received experimental diets, which consisted of: Diet low in PP; Diet low in PP with phytase (500FTU kg-1); Diet with a high PP and Diet with a high PP with phytase (500FTU kg-1). At 22 and 32 days of age two birds were slaughtered in order to collect gizzard, heart, liver, cecum,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bird; Enzyme; Substrate; Wheat bran.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001858 |
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| Naves,Luciana de Paula; Rodrigues,Paulo Borges; Teixeira,Levy do Vale; Bertechini,Antônio Gilberto; Alvarenga,Renata Ribeiro; Bernardino,Verônica Maria Pereira; Santos,Luziane Moreira dos; Natividade,Yuri Pereira Efrem. |
| The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aviculture; Bone ash; Enzyme; Metabolism; Performance; Phytate. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801479 |
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| Dadalt,Julio Cezar; Ribeiro,Andréa Machado Leal; Kessler,Alexandre de Mello; Wesendonck,William Rui; Bockor,Luciane; Gomes,Gilson Alexandre. |
| The objective of this study was to evaluate nutritional and energetic value of rice by-products, with or without phytase, using growing pigs. Thirty-six male pigs were housed in individual metabolic cages. Total collection of feces and urine was carried out in two periods of ten days: five days for adaptation and five days for collection. A randomized blocks design was used, considering the sampling period as a block, with five treatments and seven replicates. Two control diets (with and without phytase - Phy) were used in the digestibility calculations, the latter in order to evaluate the enzyme influence on energy digestibility of the tested ingredients. The control diet was replaced by 30% of one of the ingredients: defatted rice bran (DRB) with and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Alternative ingredients; Dephytinization; Enzyme; Nutrients. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701305 |
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| Nagata,Adriano Kaneo; Rodrigues,Paulo Borges; Alvarenga,Renata Ribeiro; Zangeronimo,Márcio Gilberto; Donato,Daniella Carolina Zanardo; Silva,José Humberto Vilar da. |
| The aim of this study was to evaluate carcass characteristics and quality of breast meat after the inclusion of phytase in broiler diets with different levels of apparent metabolizable energy (AMEn) corrected for nitrogen and reduced crude protein (CP) supplemented with essential amino acids following the concept of ideal protein. A total of 1,500 Cobb broilers from 22 to 42 days (initial weight of 833 ± 7 g and final weight of 2741 ± 48 g) were distributed in completely randomized design in a factorial 3x3+1 experiment (three levels of AMEn - 2,950; 3,100 and 3,250 kcal/kg - and three levels of CP - 14, 16 and 18% - and an additional treatment - control without phytase, with 3,100 kcal/kg EMAn, 19.2% PB and 0.4% available phosphorus), in six replications... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Available phosphorus; Dietary addictive; Enzyme; Ideal protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300020 |
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| Silva,Hunaldo Oliveira; Fialho,Elias Tadeu; Freitas,Rilke Tadeu Fonseca de; Lima,José Augusto de Freitas; Logato,Priscila Rosa Vieira; Schoulten,Neudi Artemio. |
| It was intended with the present work to verify the effect of phytase on the performance, bone mineralization and content of plasma urea of swine. 80 swine (30 ± 3.1 kg/LW), crossbred (LD x LW) males and females, allocated to a randomized block design (RBD) with four treatments and five replicates. The treatments consisted of a growing swine diet on the basis of corn, soybean meal and defatted rice bran (DRB) supplemented with four levels of phytase (0, 400, 800 and 1200 FTU/kg) The diet was formulated to meet the requirements of growing swine except for available calcium and phosphorus, the levels of which were 0.64 and 0.25%, respectively. The enzyme phytase utilized was Natuphos 5000. Over the experimental period every eight days, blood of two animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme; Minerals; Plasma; Urea. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600028 |
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| Gomide,Elisangela Minati; Rodrigues,Paulo Borges; Zangeronimo,Márcio Gilberto; Bertechini,Antônio Gilberto; Santos,Luziane Moreira dos; Alvarenga,Renata Ribeiro. |
| The objective of this study was to evaluate the effect of reduced-crude protein (CP) and available phosphorus (aP) diets, added with phytase and amino acids for broilers in the growing (14 to 21 days old) and retirement (35 to 42 days) phases on nitrogen, potassium and phosphorus balance. Ninety Cobb broilers were distributed in a completely randomized design with three treatments and six replications with five and three birds per experimental unit in the growing and retirement phases, respectively. Diets were elaborated to contain reduced CP and aP levels (19 and 17% CP in the growing phase, with 0.34% aP; and 18 and 16% CP in the retirement phase, with 0.30% aP), supplemented with crystalline amino acids and 500 FTU of phytase/kg diet. These were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme; Phytate; Environment; Metabolism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300022 |
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| Registros recuperados: 76 | |
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