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Registros recuperados: 222 | |
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Beatrice A. Golomb. |
Background: Overlapping chronic multisymptom illnesses (CMI) include Chronic Fatigue Syndrome (CFS), fibromyalgia, irritable bowel syndrome, multiple chemical sensitivity, and Gulf War illness (GWI), and subsets of autism spectrum disorder (ASD). GWI entails a more circumscribed set of experiences that may provide insights of relevance to overlapping conditions.
Objectives: To consolidate evidence regarding a role for oxidative stress and mitochondrial dysfunction (OSMD), as primary mediators in CMI, using GWI as a departure point.
Methods: Exposure relations, character, timecourse and multiplicity of symptoms, and objective correlates of GWI are compared to expectation for OSMD. Objective correlates of OSMD in GWI and... |
Tipo: Manuscript |
Palavras-chave: Immunology; Molecular Cell Biology. |
Ano: 2012 |
URL: http://precedings.nature.com/documents/6847/version/1 |
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Ekaterina A. Litvinova; Elena P. Goncharova; Alla M. Zaidman; Marina A. Zenkova; Mikhail P. Moshkin. |
Scent of receptive females as signal to reproduction stimulate male mice to olfactory search of a potential breeding partner^1, 2^. This searching behavior is coupled with infection risk due to bacterial contamination of the fecal and urine scent marks^4^. The theoretical consideration of host evolution under inevitable parasitic pressures, including helminthes, bacteria, virus etc., predicts adaptations that help protect against parasites associated with breeding^7^. In this study, we propose that acceptation of female signals by male mice leads to adaptive redistribution of immune defense directed to protection against respiratory infection risks. Our results reveal migration of macrophages and neutrophils to upper airways upon exposure to female odor... |
Tipo: Manuscript |
Palavras-chave: Ecology; Immunology. |
Ano: 2009 |
URL: http://precedings.nature.com/documents/3311/version/1 |
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Miao He; Lisa Kratz; Jushua Michel; Abbe Vallejo; Laura Ferris; Richard Kelley; Jacqueline Hoover; Michael Gibson; Gerard Vockley. |
Disorders of cholesterol biosynthesis have clinical manifestations involving skeleton, eyes, neurologic development, and skin. We describe a patient with congenital cataracts, developmental delay, microcephaly, and low serum cholesterol who developed severe psoriasiform dermatitis and arthralgias beginning at age 3. Her brain MRI indicatedminor gliosis. Quantitative sterol analysis of patient plasma and skin showed marked elevation of 4alpha-methyl- and 4, 4'-dimethylsterols, indicating a deficiency in the first step of sterol C4 demethylation in cholesterol biosynthesis. Molecular studies showed mutations in _SC4MOL_, a gene predicted to encode a sterol C4 methyl oxidase. Thus, our patient has a previously undescribed inborn error of cholesterol... |
Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Immunology. |
Ano: 2008 |
URL: http://precedings.nature.com/documents/2163/version/1 |
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Haiying Xie; Weiming Wang; Ziqiang Xie; Hong Chen; Kexian Chen. |
Complement anaphylatoxin 5a (C5a) has been recognized as a potent therapeutic target for anti-inflammatory therapy, thus, blocking the action of C5a on its binding receptors may provide an effective treatment of a variety of inflammatory diseases. However, there have been few clinically available non-peptide C5a receptor antagonists disclosed at present. In pursuit of better anti-inflammatory drugs, quantitative structure–activity relationship studies were carried out in a series of non-peptide C5a receptor antagonists with binding activity using different physicochemical descriptors. The conventional best 2D-QSAR models were developed using a training set of 35 molecules and an external test set of 8 molecules by genetic function approximation... |
Tipo: Manuscript |
Palavras-chave: Chemistry; Immunology; Pharmacology; Bioinformatics. |
Ano: 2011 |
URL: http://precedings.nature.com/documents/6630/version/1 |
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Christopher Carter. |
The XMRV retrovirus has been implicated in chronic fatigue syndrome and prostate cancer. A homology search comparing retroviral with human proteins revealed short contiguous amino acid strings (typically 5-8 aa) matching human proteins whose dysfunction might be expected to cause fatigue, including mitochondrial proteins related to oxidative phosphorylation, glutamate receptors and their synaptic scaffolds, muscular acetylcholine receptor scaffolds and structural proteins, components of the immune system, and phosphatidylinositol signalling inter alia. Viral proteins are also homologous to members of the oestrogen, peroxisome proliferator, and CREB activated receptor networks, all of which are implicated in prostate cancer, and to a protein, SRCAP, that... |
Tipo: Manuscript |
Palavras-chave: Cancer; Immunology; Microbiology; Neuroscience. |
Ano: 2010 |
URL: http://precedings.nature.com/documents/4669/version/1 |
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Nandkumar Kamat; Sonashia Velho-Pereira. |
New molecule discovery from natural sources, such as that of actinobacteria, has proved to be an interesting area in antibiotic research, as most of these antibiotics are difficult to synthesize. Out of 30 actinobacterial cultures screened for antimicrobial activity, 28 cultures were found to produce active products against various pathogenic microorganisms such as Gram-negative, Gram-positive bacteria and yeast, using a ‘modified cross streak method.' The modified method helped in easy quantification of results and also in ruling out probable mutual antibiosis. 53%, 13% and 10% of tested actinobacterial strains belonging to Streptomyces, Micromonospora and Actinomadura genera, respectively, showed the ability of producing antimicrobial... |
Tipo: Manuscript |
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Microbiology; Pharmacology; Bioinformatics. |
Ano: 2012 |
URL: http://precedings.nature.com/documents/6765/version/1 |
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Richard T. O'Neil; Qiaozhi Wei; Brian G. Condie. |
Thymic epithelial cells (TECs) are required for the development and differentiation of T cells and are sufficient for the positive and negative selection of developing T cells. Although TECs play a critical role in T cell biology, simple, efficient and readily scalable methods for the transfection of TEC lines and primary TECs have not been described. We tested the efficiency of Nucleofection for the transfection of 4 different mouse thymic epithelial cell lines that had been derived from cortical or medullary epithelium. We also tested primary mouse thymic epithelial cells isolated from fetal and postnatal stages. We found that Nucleofection was highly efficient for the transfection of thymic epithelial cells, with transfection efficiencies of 30-70% for... |
Tipo: Manuscript |
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Molecular Cell Biology. |
Ano: 2011 |
URL: http://precedings.nature.com/documents/6283/version/1 |
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Stephanie Balor; Marc Daëron; Pierre Bruhns; Denis Hudrisier. |
T cells acquire various proteins from their cellular partners by the process of trogocytosis. We recently demonstrated that the FcγRIIIA receptor and its associated FcRγ are captured by T cells during their co-culture with FcγR-expressing target cells upon both antigen- or antibody-mediated stimulation. Interestingly, we found that FcR captured by T cells could bind ligands but did not transmit detectable intracellular signals or signaling-depending functions upon ligand binding suggesting their improper integration in the recipient T cell membrane. In this study, we provide morphological data in support of this hypothesis. Indeed, we show that the FcRγ-subunit, which we used as a fusion to GFP, was clearly present... |
Tipo: Manuscript |
Palavras-chave: Immunology. |
Ano: 2009 |
URL: http://precedings.nature.com/documents/3824/version/1 |
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Jhinuk Chatterjee. |
Investigation and experimental design of the study was basically aimed at developing insight into the antigenicity of spermatozoa-associated proteins. Apart from studying the natural antigenicity of washed whole spermatozoa, their immunogenicity was also demonstrated _in vitro_. The whole live spermatozoa were immobilized and agglutinated _in vitro_ by the antibodies they induced in the laboratory model - a female rabbit. A regular immunization routine induced a high titre of antisperm polyclonal antibodies. To prepare a spermatozoa specific antigen which will not produce a cross-reacting antibody against other human tissues, only the motile and live spermatozoa were selected for antigen preparation. In investigation the laboratory-bred female rabbits were... |
Tipo: Manuscript |
Palavras-chave: Immunology. |
Ano: 2008 |
URL: http://precedings.nature.com/documents/1855/version/1 |
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Peter Natesan Pushparaj; Jayapal J. Manikandan; Alirio Jose A. J. Melendez. |
Severe acute respiratory syndrome (SARS) emerged in 2003, as a new epidemic form of life-threatening infection. As of 26th September 2003, there were 8422 cases of SARS from 29 countries with 908 deaths (WHO). However, the pathogenesis of SARS is poorly understood. To understand the host response to this pathogen, we profiled the gene expression patterns of peripheral blood mononuclear cells (PBMC) from SARS patients compared to healthy controls using one of the latest techniques, high density oligonucleotide expression probe array (HG-Focus array, Gene Chip, Affymetrix, Santa Clara, CA). High-density oligonucleotide microarray is a promising approach for high throughput analysis. It has been extensively
used in many areas of biomedical... |
Tipo: Poster |
Palavras-chave: Immunology; Molecular Cell Biology; Bioinformatics. |
Ano: 2007 |
URL: http://precedings.nature.com/documents/1145/version/1 |
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Alexandra J. Burton; Daryl V. Nydam; Gary Jones; Jennifer Zambriski; Thomas C. Linden; Graham Cox; Randy Davis; Alicia Brown; Dwight D. Bowman. |
Cryptosporidium parvum is a zoonotic apicomplexa-protozoan pathogen that causes gastroenteritis and diarrhoea in mammals worldwide. The organism is transmitted by ingestion of oocysts, which are shed in faeces, and completes its lifecycle in a single host.^1^ C. parvum is ubiquitous on dairy operations worldwide and is one of the leading causes of diarrhoea in calves on these farms.^2,3^ Here, for the first time, we describe the antibody response in a large group of cows to a recombinant C. parvum oocyst surface protein (rCP15/60) vaccine and the antibody response in calves fed rCP15/60-immune colostrum produced by these vaccinated cows. Results of recent genotype surveys indicate that calves are the only major reservoir for C. parvum infections in... |
Tipo: Manuscript |
Palavras-chave: Immunology. |
Ano: 2009 |
URL: http://precedings.nature.com/documents/3988/version/1 |
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S.S. Soam; Feroz Khan; Bharat Bhasker; B. N. Mishra. |
The identification of major histocompatibility complex (MHC) class-II restricted peptides is an important goal in human immunological research leading to peptide based vaccine design. These MHC class–II peptides are predominantly recognized by CD4+ T-helper cells, which when turned on, have profound immune regulatory effects. Thus, prediction of such MHC class-II binding peptides is very helpful towards epitope-based vaccine design. HLA-DR proteins were found to be associated with autoimmune diseases e.g. HLA-DRB1*0401 with rheumatoid arthritis. It is important for the treatment of autoimmune diseases to determine which peptides bind to MHC class II molecules. The experimental methods for identification of these peptides are both time consuming... |
Tipo: Manuscript |
Palavras-chave: Immunology; Bioinformatics. |
Ano: 2011 |
URL: http://precedings.nature.com/documents/6608/version/1 |
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Mehdi Shafiee Ardestani; Hasan Mehrab; Amin Geravand; Nasir Mohajer; Mostafa Saffari. |
Many scientific efforts have been well done to investigate the effects of anti inflammatory agents on the degenerative brain diseases such as Parkinson’s (PD) or Alzheimer’s disease and their affiliated sings. Previously we showed the effectiveness of steroids on rigidity of PD and in the study for further mechanistic investigation of that observation the microdialysis technique was employed to determine the striatal dopamine changes in parkinsonian rats after administration of betamethasone (0.12, 0.24 mg/kg) respectively. Our findings showed us the significant increase in the striatal dopaminergic neurotransmission (P<0.05) after administration of betamethasone comparing to the controls. These observations suggest a new... |
Tipo: Manuscript |
Palavras-chave: Chemistry; Immunology; Neuroscience; Pharmacology. |
Ano: 2008 |
URL: http://precedings.nature.com/documents/2330/version/1 |
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Wan-Jiung(Wan-Chung) Hu. |
Alzheimer's disease is a common neurodegenerative disorder. However, its exact etiology is still unknown. There were several mechanisms proposed such as the tau hypothesis and amyloid hypothesis. However, there is evidence challenging the above two hypotheses. Here, I propose the immune-amyloid hypothesis as a mechanism for Alzheimer's disease. Th17 related autoimmunity contributes to the disease pathogenesis. Accumulation of misfolded beta amyloid can trigger heat shock protein which in turn induces TH17 immunity. By microarray analysis of peripheral blood mononuclear cells, there is up-regulation of many TH17 related molecules after Alzheimer's disease. After knowing the exact disease pathogenesis, we can develop new... |
Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Immunology; Neuroscience; Bioinformatics. |
Ano: 2011 |
URL: http://precedings.nature.com/documents/5934/version/1 |
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Registros recuperados: 222 | |
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