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Evidence for natural hybrtidisation between the two oyster sub-species Crassostrea gigas and Crassostrea angulata in Southern Europe ArchiMer
Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie.
Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe during the XVIth and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI). This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier...
Tipo: Text Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf
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Gametogenesis, spawning behavior and larval abundance of the Pacific oyster Crassostrea gigas in the Thau lagoon: Evidence of an environment-dependent strategy ArchiMer
Ubertini, Martin; Lagarde, Franck; Mortreux, Serge; Le Gall, Patrik; Chiantella, Claude; Fiandrino, Annie; Bernard, Ismael; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle.
The Thau lagoon, located at the South of France, provides 10% of the French pacific oyster Crassostrea gigas production. Despite this intensive shellfish production, the larval recruitment of this species within the Thau lagoon is still misunderstood and spat collection remains highly variable; as a consequence, shellfish farmers rely mostly on hatchery”s spat. The present study aimed to describe reproduction features of C. gigas within the Mediterranean, as well as to explore spawning triggers of this species. To achieve this goal, the reproduction cycle from gametogenesis to spawning was followed during two years in several locations within the Mediterranean Thau lagoon. The spawning behavior of C. gigas within the Thau lagoon appeared to be slightly...
Tipo: Text Palavras-chave: Mollusc physiology; Spawning triggers; Oysters.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00369/48032/48118.pdf
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Situation of wild beds of Crassostrea gigas regarding OsHV-1 µvar: spatiotemporal description of contamination in Pacific oyster wild beds in Marennes Oleron Bay ArchiMer
Pepin, Jean-francois; Soletchnik, Patrick; Lupo, Coralie; Geairon, Philippe; Seugnet, Jean-luc; Robert, Stephane; Bernard, Ismal; Le Moine, Olivier.
This study focuses on the situation of wild oyster beds of Crassostrea gigas against OsHV-1 μvar and presents a spatio-temporal description of the OsHV-1 contamination in wild oyster beds in the bay of Marennes-Oléron from a range of sampling based on a hydrodynamic model and analyzes conducted in 2010. The objectives were to describe wild populations of pacific oysters in Marennes Oleron bay; to characterize these populations regarding OsHV-1 prevalence for spat and adults in connection with the isolation level from reared stocks and the sampling periods.
Tipo: Text Palavras-chave: Virus; OsHV-1; Contamination; Oysters; Crassostrea gigas; Spatiotemporal description; Marennes Oleron Bay.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00105/21673/19251.pdf
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Activité respiratoire en continu de l'huître Crassostrea gigas : mise au point d'une nouvelle méthodologie ArchiMer
Bougrier, Serge; Geairon, Philippe.
A continuous monitoring system to measure the respiration of Crassostrea gigas has been developped. The metabolic function estimated by oxygen consumption has been monitored during periods of 24 h, without food, at a temperature constant of 15°C. Preliminary results showed that the mean percentage of oyster activities was 50 % with a high variability from 0 to 100 %. The number of activity periods per day (24 h) is function of the oysters with variation between 0 and 7.
Tipo: Text Palavras-chave: Physiology; Respiration; Crassostrea gigas; Oysters; Physiologie; Respiration; Crassostrea gigas; Huîtres.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3046.pdf
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Summer mortality of the Pacific cupped oyster Crassostrea gigas in the Bay of Marennes-Oleron (France). Spatial variability of environmental parameters, growth and production rates using a Geographical Information System (GIS) ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe; Robert, Stephane.
The Marennes-Oleron Bay is the first rearing area in Europe for the cupped oyster, with 35 000 tons proudced on a yearly basis. This species has suffered several chronic summer mortalities for the last 10 years.
Tipo: Text Palavras-chave: Marennes Oleron Bay; Geogaphical Information System; GIS; Production; Growth; Crassostrea gigas; Oysters; Summer mortality.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3376.pdf
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Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis ArchiMer
Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf
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Oyster population genetics : understanding natural populations and tracing introductions ArchiMer
Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf
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Evaluation of oyster sampling efficiency of patent tongs and an oyster dredge ArchiMer
Chai, Ai-ling; Homer, Mark; Tsai, Chu-fa; Goulletquer, Philippe.
Sampling efficiency of two oyster fishing gears, patent tongs and an oyster dredge, were compared in reference to diver-harvested quadrats in Chesapeake Bay, which supports important harvests of eastern oyster Crassosrrea virginica. Mean densities of spat (0$35 mm), small oysters (>35 mm to 75 mm), marketable oysters (>75 mm), and all oysters (three size-groups combined) estimated from patent tong samples were not significantly different from those derived from diver-harvested quadrat samples. In contrast, the densities estimated from dredge samples were low, only 2-32% of the diver estimates. Accordingly, patent tongs are recommended as the sampling gear for estimating eastern oyster stock abundance in the Maryland portion of Chesapeake Bay.
Tipo: Text Palavras-chave: Chesapeke Bay; Sampling efficiencies; Crassostrea virgnica; Oysters.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-3065.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf
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Development of SNP genotyping arrays in two shellfish species ArchiMer
Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C..
Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped...
Tipo: Text Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf
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Infestation des coquilles d'huîtres Crassostrea gigas par les polydores en Basse-Normandie: recommandations et mise au point d'un traitement pour réduire cette nuisance. ArchiMer
Ruellet, Thierry.
Polydorid species form a complex of 157 Spionidae. Among them, Boccardia polybranchia, Boccardia semibranchita, Polydora ciliata and Polydora hoplura regularly colonize oysters cultivated in Normandy. Only one tenth of the populations survives more than one year. Recruits observed on the East coast of Cotentin come from the rocky calcareous flatness of Calvados. Their reproduction is spread out over all the year but winter recruitment is more prejudicial for oysters. Deepest area are more infested. Polydorids create mudblisters which cause a bad marketing of the oyster production. In order to reduce this harmful effect, it is possible to shift the setting of the oyster towards the end of March, to transfer oysters towards other areas during the peak of...
Tipo: Text Palavras-chave: Boccardia; Polydora; Parasites control; Polychaeta; Pests; Oysters; Boccardia; Polydora; Parasites (lutte contre les); Polychètes; Animaux et plantes nuisibles; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-162.pdf
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Copper levels evolution in Arcachon bay oysters : role of the regulation on antifouling paints ArchiMer
Alzieu, Claude; Barbier, Georges; Sanjuan, Jane.
The ban of tributyltin compounds in antifouling paints induced a strong decrease in the level of tin in Arcachon bay. At the same time, copper inputs have increased tenfold since 1982 owing to the widespread use of paints containing cuprous oxide. In spite of this large input, the statistical analysis of monitoring data (1979-1985) show a relative stability in the copper levels in Arcachon bay oysters.
Tipo: Text Palavras-chave: Cuivre; Huîtres; Bassin d'Arcachon; Peintures antisalissures; Copper; Oysters; Arcachon Bay; Antifouling paints.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00022/13291/10337.pdf
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Huîtres et norovirus : une affinité particulière? ArchiMer
Le Guyader, Soizick; Maalouf, Haifa; Le Pendu, Jacques.
Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of genogroup I strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to...
Tipo: Text Palavras-chave: Huîtres; Norovirus; Ligand; Sélection de souches; Oysters; Norovirus; Ligand; Strain selection.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26357/24514.pdf
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Réponses et adaptations de la charge énergétique adénylique et l'activité enzymatique digestive à l'emersion chez Crassostrea gigas ArchiMer
Moal, Jeanne; Samain, Jean-francois.
The effect of tidal emergence on the adenylate energy charge value was studied in Crassostrea gigas oysters in Marennes-Oleron Bay. The shot-term AEC responses to aerial exposure differed according to the period of the year. However, on a long-term basis, osters from three bathymetric positions exhibited the same AEC value, but had a different growth rate. The amylase increase observed for the most emerged oysters may play a role in the maintenance of energy equilibrium.
Tipo: Text Palavras-chave: Charge énergétique; Amylase; Emersion; Huîtres; Crassostrea gigas; Cycle de marée; ATP; Adenylic energy charge; Amylase; Emersion; Oysters; Crassostrea gigas; Tidal cycle.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00022/13285/10331.pdf
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Approche de la capacité trophique d'un écosystème conchylicole ArchiMer
Heral, Maurice; Deslous-paoli, Jean-marc; Prou, Jean.
After a short bibliographical study of the different approaches for determination of the trophic capacity of a bay for molluscs culture, the authors, with a global mode, show the relations between the growth performance of the oysters Crassostrea gigas in relation with the cultivated stocks. The volumes of consumed and assimilated food are calculated for the stocks in relation with the incoming volumes of water in the bay and the residence time for neap tide and spring tide. The authors find a decrease of growth rate in relation with the volume of consumed food. When it is above 60% and when the volume of assimilated food is above 20%, there is a large decrease of the growth of oysters.
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Stock assessment; Models; Food consumption; Growth; Biomass; Stock cultivé; Biomasse; Croissance; Modèle; Crassostrea gigas; Huîtres; Capacité trophique.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/acte-3834.PDF
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Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture ArchiMer
Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf
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Relationship between valve activity, microalgae concentration in the water and toxin accumulation in the digestive gland of the Pacific oyster Crassostrea gigas exposed to Alexandrium minutum ArchiMer
Haberkorn, Hansy; Tran, Damien; Massabuau, Jean-charles; Ciret, Pierre; Savar, Veronique; Soudant, Philippe.
The complexity of the relationships between Alexandrium minutum (Am.) concentration in the water ([A.m.](w)), Paralytic Shellfish Poisoning contamination in the digestive gland ([PSP](dg)) and valve behavior was explored in oysters Crassostrea gigas. Two experiments were conducted, during which oysters' valve behaviour were analyzed. Oysters, first acclimated for 10-days with the non harmful microalgae Heterocapsa triquetra (H.t.), were exposed to four microalgae mixtures at constant total concentrations of 10 x 10(3) cells ml(-1) (experiment-1) and 5 x 10(3) cells ml(-1) (experiment-2): 100% Am.; 50% A.m.-50% H.t.; 25% A.m.-75% H.t.; 100% H.t. At the end of experiment-2, [PSP](dg) were measured. At 10 x 103 cells ml(-1), the microalgal ingestion decreased...
Tipo: Text Palavras-chave: Harmful algae; Oysters; Behavioral responses; Algae concentration; Toxin accumulation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00041/15195/12617.pdf
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Evolutions quantitatives et qualitatives du seston dans une claire à huîtres : relation avec la sédimentation et la biodéposition ArchiMer
Sornin, Jean-marc; Delmas, Daniel; Deslous-paoli, Jean-marc.
Dans un bassin aquacole fermé (claires à huîtres), l'évolution qualitative et quantitative des matières en suspension subit des variations saisonnières directement liées aux capacité de production planctonique. Ainsi, à la fin du printemps et en été, le réchauffement des eaux s'accompagne d'une augmentation de matière organique importante (jusqu'à 17 mg.1(-1). Pendant cette période, la sédimentation naturelle est supérieure à 350 g.m(-2).j(-1). La biodéposition (fèces + pseudofèces) suit l'évolution des capacités de rétention des huîtres. Dans ce type de bassin fermé, cette capacité est liée à la qualité des matières en suspension. En période estivale, les fortes teneurs en matière organique du seston (entre 5 et 17 mg.1(-1)) dispensent les mollusques...
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Biodeposition; Sedimentation; Suspension; Particulate organic matter; Crassostrea gigas; Huîtres; Biodéposition; Sédimentation; Suspension; Matière organique particulaire.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/publication-7309.pdf
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Amylase polymorphism affects growth in the cupped oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Samain, Jean-francois; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Van Wormhoudt, A..
The better understanding of physiological and environmental factors that determine optimal food conversion efficiencies is of major interest for the cupped oyster Crassostrea gigas for which the strong increase of aquaculture has been correlated in France with a decrease in productivity due to competition between aquatic species for limited food supplies at grow-out sites. To investigate the non-neutrality of the polymorphism of amylase, a key enzyme for carbohydrate assimilation, in oyster physiology, we report in the present paper the growth and survival measurement of amylase genotypes within five bi-parental C. gigas families, bred to be polymorph at the two PCR-RFLP amylase markers, and reared over one year under standard culture conditions in two...
Tipo: Text Palavras-chave: Amylase markers; Growth; Amylase Polymorphism; Genetic; Oysters; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3311.pdf
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Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles ArchiMer
Gonzalez-fernandez, Carmen; Tallec, Kevin; Le Goic, Nelly; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Suquet, Marc; Berchel, Mathieu; Paul-pont, Ika.
While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the...
Tipo: Text Palavras-chave: Nanoplastics; Oysters; Gametes; Cellular responses.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00443/55459/56976.pdf
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