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| Registros recuperados: 100 | |
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| Camargos,A.; Wohlres-Viana,S.; Costa,I.F.; Camargo,L.S.; Ferreira,J.C.; Ramos,A.A.; Oba,E.. |
| ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein-Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P<0.05) but no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PTGFR; ESR1; Progesterone; Estradiol; RT-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400821 |
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| Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.. |
| ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077 |
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| Pilz,D.; Alfieri,A.F.; Lunardi,M.; Alfieri,A.A.. |
| Utilizou-se a técnica da RT-PCR para a detecção da região 5' UTR do genoma do vírus da diarréia viral bovina (BVDV) em pools de soros sangüíneos provenientes de um rebanho, constituído por 226 animais, que apresentava distúrbios da reprodução. A partir das amostras individuais de soro e de acordo com a categoria dos animais e o número de animais por categoria foram formados 10 pools (A a J) de soros. A primeira avaliação revelou a amplificação de um produto com 290pb nas reações referentes aos grupos D (35 vacas) e H (25 bezerros lactentes) que, após o desmembramento em amostras individuais, resultou na identificação de 11 vacas lactantes e 12 bezerros em amamentação positivos. Para a identificação de animais persistentemente infectados (PI) entre os 23... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Diarréia viral bovina; BVDV; RT-PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100001 |
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| Batista,J.J.; Martins,A.S.; Moro,L.; Vasconcelos,A.C.. |
| Determinou-se a expressão gênica das caspases 3 e 8 mediante transcrição reversa de mRNA total e reação em cadeia da polimerase (RT-PCR) para avaliar a apoptose em timo e baço de ratas imunossuprimidas por glicocorticóides. Utilizou-se dexametasona para indução da apoptose e atrofia linfóide. Quarenta e cinco fêmeas Wistar recém-desmamadas foram separadas em três grupos: as ratas de A (n=18) e B (n=18) foram tratadas com 250 e 500mg de glicocorticóide, via intramuscular, respectivamente, e as do C (n=9) não foram tratadas. Após 24, 48 e 72 horas, seis animais de cada grupo tratado e três do controle foram anestesiados, pesados e sacrificados. O baço e o timo foram coletados e pesados. Fragmentos dos órgãos foram fixados em formol tamponado a 10% e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rato; Apoptose; Dexametasona; Timo; Baço; Caspase 3; Caspase 8; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400006 |
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| Momtaz,H.; Rahimi,E.; Hajian,S.; Momeni,M.. |
| The objective of this study was to determine the prevalence of Hepatitis A Virus (HAV) in sea food samples in the Isfahan and Shahrekord townships in Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in the Isfahan and Shahrekord townships in Iran. The samples were tested for the presence of HAV using a reverse transcriptase- polymerase chain reaction method. Out of the total number of samples examined, 8 (2.7%) were found to be positive for HAV. This virus was detected in 5% and 1.7% of fresh fish and shrimp, respectively. This study shows the importance of sea food as potential sources of HAV infection in people in Iran. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HAV; Sea food; RT-PCR; Iran. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400015 |
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| Negrão,F.J.; Wosiacki,S.H.; Alfieri,A.A.; Alfieri,A.F.. |
| Fragmentos com 721 pares de bases do gene da hemaglutinina (H) do vírus da cinomose canina (CDV), amplificados pela RT-PCR a partir de três estirpes vacinais (Snyder Hill, Onderstepoort e Rockborn) e de 27 amostras de campo, provenientes de cães com cinomose, foram clivados com as endonucleases Hinf I and Rsa I. A seleção das enzimas foi realizada por meio de análises in silico de seqüências do CDV depositadas em bases públicas de dados. Tanto as estirpes vacinais quanto as amostras selvagens do CDV apresentaram com a enzima Hinf I o mesmo perfil de restrição, confirmando a identidade do fragmento amplificado pela RT-PCR, uma vez que todas as estirpes com seqüências disponíveis (GenBank) têm sítios de restrição para essa enzima nas mesmas posições. O... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Cinomose; Vírus da cinomose canina; Gene H; RT-PCR; RFLP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600019 |
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| Gebara,C.M.S.; Wosiacki,S.R.; Negrão,F.J.; Oliveira,D.B de; Beloni,S.N.E.; Alfieri,A.A.; Alfieri,A.F.. |
| A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, precedida de transcrição reversa (RT-PCR), em 87 amostras de urina de cães que apresentavam sinais clínicos sugestivos de cinomose. Os animais foram distribuídos em três grupos. No grupo A foram incluídos 41 cães com alterações sistêmicas; no grupo B, 37 cães com alterações neurológicas; e no grupo C, nove cães com alterações sistêmicas e neurológicas simultâneas. O grupo D (controle) foi composto por 20 cães assintomáticos. Os resultados da RT-PCR foram correlacionados com a forma clínica da infecção e com as alterações hematológicas encontradas. Foi possível a amplificação parcial do gene da nucleoproteína do CDV em 41 (47,1%) das 87 amostras de urina... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cinomose canina; Vírus da cinomose; Urina; RT-PCR. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400009 |
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| Castilho,J.G.; Brandão,P.E.; Carnieli Jr,P.; Oliveira,R.N.; Macedo,C.I.; Peixoto,Z.M.P.; Carrieri,M.L.; Kotait,I.. |
| Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Canine distemper virus; RT-PCR; Phylogeny. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016 |
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| Radulović,.; Milutinović,M.; Tomanović,S.; Mulenga,A.. |
| A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015 |
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| Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R. |
| Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine... |
| Tipo: Journal article |
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005 |
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| MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
| Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
| Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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| DHINGRA,SANJIV; BANSAL,MOHINDER P. |
| Type-1 5'-iodothyronine deiodinase (5'-DI) is responsible for conversion of T4 to T3. Selenium (Se) is an integral part of this enzyme. Keeping in view the strong association between atherosclerosis and hypothyroidism, the present study examined the behavior of 5'-DI in liver, aorta and thyroid during hypercholesterolemia following different Se status, i.e., Se deficiency (0.02ppm), adequate (0.2ppm) and excess dose (1ppm) in SD male rats. Animals were fed a control or high-cholesterol diet (2%) for 1 and 2 months. 5'-DI activity and mRNA expression was measured by RIA and RT-PCR respectively. In liver and aorta, 5'-DI expression significantly decreased with the Se-deficient and the high-cholesterol diet. The trend was opposite in thyroid, i.e., mRNA... |
| Tipo: Journal article |
Palavras-chave: Selenium; Hypercholesterolemia; 5'-DI; Cholesterol; RT-PCR. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200013 |
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| Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo. |
| From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008 |
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| Yildirim,Aysegul Burcin; Karabulut,Derya; Dundar,Munis; Ulusoy,Hasan Basri; Sonmez,Mehmet Fatih. |
| The aim of this work was to study the relative ghrelin and growth hormone secretagogue receptor (GHS-R)1a gene expression in the kidney of long-term diabetic rats. Forty male Wistar albino rats were divided into four groups: C- control group, DI- one month diabetic rats group, DII- two months diabetic rats group, and DIII- three months diabetic rats group. Diabetes was induced by streptozotocin STZ (40mg/kg i.p). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue GHS-R mRNA levels, ghrelin expression, and histopathological damage scores were compared. Dilatation in the distal tubules, epithelial desquamation into the lumen of the tubules and transparent tubules showing glycogen vacuolation were observed in all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes mellitus; Ghrelin; GHSR-1a; Kidney; Immunohistochemistry; RT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100300 |
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| Gomes,Maria de Lourdes C.; Ferreira,Lauze Lee A.; Gomes,Rodrigo Henryque; Lamarão,Letícia M.; Silveira,Edna da; Rodrigues,Lílian da Silva; Silva,Cláudio Ferreira da; Almeida,Eduardo Ribeiro de. |
| Echovirus (Echo) 30 or human enterovirus B is the most frequent enterovirus associated with meningitis cases. Epidemics and outbreaks of this disease caused by Echo 30 have occurred in several countries. In Brazil, Echo 30 has been isolated from sporadic cases and outbreaks that occurred mainly in the south and southeast regions. We used RT-PCR to examine Echo 30 isolates from meningitis cases detected from March 2002 to December 2003 in Belém, state of Pará, in northern Brazil. The patients were attended in a Basic Health Unit (State Health Secretary of Pará), where cerebrospinal fluid (CSF) was collected and stored in liquid nitrogen. Weekly visits were made by technicians from Evandro Chagas Institute to the health unit and samples were stored at -70ºC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Echovirus 30; Aseptic meningitis; RT-PCR; Belém; Brazil. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400006 |
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| Registros recuperados: 100 | |
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