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Registros recuperados: 65
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Semen and reproductive parameters during some abstinence periods after cigarette smoke exposure in male rats BABT
Sankako,Michele Kimie; Garcia,Patricia Carvalho; Piffer,Renata Carolina; Pereira,Oduvaldo Câmara Marques.
Cigarette smoking is very widespread globally and can also be implicated in male and female infertility. This study aimed to evaluate the testicular function throughout a complete spermatic cycle during abstinence from cigarette smoke exposure in order to identify a possible residual damage and whether the parameters could recover spontaneously. Male Wistar rats were randomly divided into control and cigarette smoke-exposed (20 cigarettes/day/2 months) groups. After finishing the treatment, according to the number of days after the last cigarette exposure (0, 15, 30, or 60 days), the rats were euthanized and analyzed for compromised sperm count and quality. Results showed residual damage on sperm concentration, motility and morphology; the recovery of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cigarette; Semen; Spermatozoa; Testicular Function; Male Rat.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100012
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Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa BABT
Vasconcelos,André Belico de; Souza,Patrícia Castanheira de; Varago,Fabiana Cristina; Lagares,Monique de Albuquerque; Santoro,Marcelo Matos.
The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microcalorimetry; Glucose; Spermatozoa; Sperm metabolism; Cell viability; Equine.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010
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Effect of two ram sperm capacitating media on acrosome reaction and zona-free hamster oocyte penetration test Braz. J. Vet. Res. Anim. Sci.
FERRARI,Silvia; BARNABE,Valquíria Hyppolito; ZÜGE,Roberta Mara; ZOGNO,Maria Amélia.
An in vitro zona-free hamster oocyte penetration test was utilized in 24 trials in order to evaluate the capacitation media used for ram sperm. A pool of fresh semen was collected from three crossed breed rams. Two semen drops were washed by centrifugation and incubated in high ionic strength treatment (HIS) or in a defined medium with HEPES, on heat ewe serum and heparin. After the incubation to promote capacitation, simplified triple-stain technique was used to evaluate the spontaneous acrosome reaction of the capacitated sperm. Superovulation in 96 golden hamsters was induced by PMSG and hCG. The oocytes were treated with hyaluronidase and trypsin to remove, respectively, the cumulus cells and the zona pellucida. Oocytes and capacitated sperm were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capacitation; Spermatozoa; Rams; Oocytes; Hamsters.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300010
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Aggravation of cyclophosphamide-induced reproductive toxicity in mice by aqueous extract of Aegle marmelos (L.) BJPS
Singh,Sangita; Mishra,Awadhesh Kumar; Lata,Swarn; Tiwari,Kavindra Nath.
ABSTRACT Aegle marmelos (L.) (Rutaceae) commonly known as bael is an important medicinal fruit tree. The present study focused on the effects of aqueous extract of Aegle marmelos (AEAM) on the testis and sperm characteristics induced by cyclophosphamide (CPA) in mice. Thirty six adult Parke’s strain mice were divided into six groups: group I given only distilled water (control); group II administered with AEAM alone once in a week for five weeks; group III administered with CPA (200 mg/kg b.w., intraperitoneally) once in a week for five weeks and group IV-VI CPA along with AEAM (400, 500 and 600 mg/kg b.w., orally). CPA was found to reduce gonadosomatic index (GSI), sperm counts, motility, viability, antioxidant activities and induced histopathological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aegle marmelos (L.)/extract/effects/sperm; Cyclophosphamide; Gonadosomatic index (GSI); Reproductive toxicity; Spermatozoa.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300614
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Fertility in the stallion as accessed by the spermatozoa morphology Ciência Rural
Pimentel,Cláudio Alves; Hammes,Anelise Maria; Dietrich,Giovani.
In a breeding farm, 129 mares were bred to 4 stallions and had an overall pregnancy rate of 76.7%. Stailions were submitted to semen examination for at least two times at the beginning of the breeding season and the percentage of morphologically normal spermatozoa were used for statistical analysis in relation to the percentage of pregnancy at the end of the breeding season. A regression analysis revealed that the percentage of pregnancy (y) can be estimated by the percentage of normal spermatozoa (x) in the ejaculate according to the following equation: y=52.38+0.44x; r2=97.8%; P<0.01. We concluded that the pregnancy rate can be estimated by the percentage of morphologically normal spermatozoa in the ejaculate of stallions, since it is an importam...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stallion; Semen; Fertility; Spermatozoa; Morphology.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000400025
Registros recuperados: 65
Primeira ... 1234 ... Última
 

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