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Registros recuperados: 115
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Infections virales chez les huîtres Crassostrea angulata Lmk. et Crassostrea gigas Th. ArchiMer
Comps, Michel; Duthoit, Jean-louis.
Two viral infections associated to some epizooties have been observed in Crassostrea angulata Lmk. The first one concerns the Gill disease in which the viral damages go hand in hand with a cellular hypertrophy, while, in the second one, the contaminated cells are associated with a deterioration of the connective tissue. These branchial necrosis associated to a virus have been observed again recently in some Portuguese oysters from the natural deposits of the Cadix (Spain) area. Moreover, the study of the summer mortalities (July 1977) in the oyster Crassostrea gigas Th. demonstrated the presence in this species of a virus of the same type as the one associated to the massive mortalities of the Portuguese oyster. In both cases, the viral morphogenesis takes...
Tipo: Text Palavras-chave: Oyster; Crassostrea gigas; Crassostrea angulata; Pathology; Virus; Huître; Crassostrea gigas; Crassostrea angulata; Pathologie; Virologie.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/1979/publication-5922.PDF
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REPAMO - REseau de PAtologie des MOlusques : Bilan des examens zoosanitaires réalisés en 1998 ArchiMer
Pichot, Yves.
Tipo: Text Palavras-chave: Virus; Parasitology; Monitoring; Bivalves; Molluscs.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/rapport-1600.pdf
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Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp ArchiMer
Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W..
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection...
Tipo: Text Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf
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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998 ArchiMer
Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf
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Fecal contamination in coastal areas: An engineering approach ArchiMer
Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf
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Etat des connaissances sur la pathologie chez les mollusques bivalves ArchiMer
Fouche, Dominique.
A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases.
Tipo: Text Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf
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Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire) 1997 ArchiMer
Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf
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Définition et test de nouvelles amorces pour la détection en PCR du virus de type herpès infectant les huîtres ArchiMer
Renault, Tristan; Lipart, Cecile.
La collaboration, entamée en 1996 avec A. Davison du Medical Research Council de Glasgow, a permis d'aboutir à l'obtention d'informations sur la équence de fragments clonés d'ADN de virus de type herpès infectant les huîtres.
Tipo: Text Palavras-chave: Détection PCR; Virologie; Virus; Herpès; Huîtres creuses; Crassostrea gigas; Techniques.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00199/31048/29465.pdf
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Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996 ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf
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Mortalités massives en élevage larvaire de Lates Calcarifer ( Mise en évidence d'un virus neurotrope ) ArchiMer
Renault, Tristan; Thouard, Emmanuel; Weppe, Maurice.
Since 1986 high mortality of hatchery-reared sea bass, Lates Calcarifer, larvae appeared in Ifremer's Pacifie Oceanological Center (Tahiti). Light and electron microscopy show vacuolation and cells with cytoplasmic inclusion bodies in the brain, the cord and the eye relina of affected larvae. Numerous non-enveloped particles identified as viral units, icosahedral in morphology and measuring about 25 nm in diameter were found in the cytoplasm of the degenerating cells. In affected larvae, these particles were isolated by density grandient centrifugations. In vitro tests on cell lines and in vivo test on fish are performed with samples of diseased larvae. And immunisation of mice are made with purified immunoglobulins of Lates calcarifer.
Tipo: Text Palavras-chave: Aquaculture; Élevage larvaire; Lates calcarifer; Pathologie; Virus; Spongiose; Aquaculture; Hatchery; Lates calcarifer; Pathology; Virus; Spongiosis.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00142/25306/23378.pdf
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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997 ArchiMer
Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf
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Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997 ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf
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Effects of experimental warming on small phytoplankton, bacteria and viruses in autumn in the Mediterranean coastal Thau Lagoon ArchiMer
Courboulès, Justine; Vidussi, Francesca; Soulié, Tanguy; Mas, Sébastien; Pecqueur, David; Mostajir, Behzad.
To investigate the responses of a natural microbial plankton community of coastal Mediterranean waters to warming, which are still poorly known, an in situ mesocosm experiment was carried out in Thau Lagoon during autumn 2018. Several microorganisms, including virio-, bacterio-, and phytoplankton < 10 µm in size, were monitored daily and analysed using flow cytometry for 19 consecutive days in six mesocosms. Three mesocosms (control) had the same natural water temperature as the lagoon, and the other three were warmed by + 3 °C in relation to the control temperature. The cytometric analyses revealed an unexpected community dominated by picophytoplanktonic cells, including Prochlorococcus-like and Picochlorum-like cells, which had not previously been...
Tipo: Text Palavras-chave: Pico- and nanophytoplankton; Bacterioplankton; Virus; Warming; In situ mesocosm; Thau lagoon.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00688/79979/82933.pdf
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Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des virus. Sous-région marine Mers celtiques. Evaluation initiale DCSMM. ArchiMer
Pommepuy, Monique.
Tipo: Text Palavras-chave: Mers celtiques; Coquillages; Virus; Risques; Sources de contamination; Impacts.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00329/43978/43550.pdf
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Recherche sur la paralysie virale du crustace decapode Macropipus depurator L. ArchiMer
Bonami, Jean-robert.
The trembling of legs followed by increasing paralysis and eventual death, were recorded on specimens of the above sp from Sete region. EM studies of tissue showed paraspherical bodies measuring approx 61 nm with a size range of 58-65 nm. By experimentally infecting specimens with a purified viral suspension the following pathogenesis was observed: in greater than 60 per cent of the specimens, trembling of the legs began within 6 days, growing very pronounced on the 8th day, leading to a general paralysis and 9 days after the infection a mortality rate of 70-80 per cent was recorded. The following characteristics of the virus are noted: its cytoplasmic localisation, paraspherical form, size ranging from 60-70 nm and the absence of an envelope. Considering...
Tipo: Text Palavras-chave: Paralysis; Virus; Decapoda.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/publication-2151.pdf
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In situ structuring of virioplankton through bacterial exoenzymatic activity: interaction with phytoplankton ArchiMer
Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene.
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using...
Tipo: Text Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf
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Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des virus. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM. ArchiMer
Pommepuy, Monique.
Tipo: Text Palavras-chave: Golfe de Gascogne; Coquillages; Virus; Risques; Sources de contamination; Impacts.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00329/44062/43633.pdf
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Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006 ArchiMer
Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf
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Microbial interactions in marine water amended by eroded benthic biofilm: A case study from an intertidal mudflat ArchiMer
Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J..
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (< 0.6 μ- and < 10 μ filtrates) were diluted either with < 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with < 30 kDa-benthic compounds (BC). The kinetics of heterotrophic...
Tipo: Text Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf
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Infecciones de Transmisión Sexual en Semen: El Hombre como Vector de Transmisión International Journal of Morphology
Viscarra A,Tamara; Brebi M,Priscilla; Andana V,Alejandra; Sánchez G,Raúl.
En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía de transmisión, debido principalmente a la afinidad de algunos patógenos con los espermatozoides. Dentro de los principales microorganismos infectantes en semen se encuentran Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Virus de la Inmunodeficiencia Humana...
Tipo: Journal article Palavras-chave: Infecciones de transmisión sexual; Semen; Virus; Bacterias; Protozoos.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100041
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