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Registros recuperados: 115 | |
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Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.. |
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection... |
Tipo: Text |
Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf |
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Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe. |
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En... |
Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf |
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Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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Renault, Tristan. |
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années... |
Tipo: Text |
Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf |
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Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie. |
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la... |
Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf |
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Courboulès, Justine; Vidussi, Francesca; Soulié, Tanguy; Mas, Sébastien; Pecqueur, David; Mostajir, Behzad. |
To investigate the responses of a natural microbial plankton community of coastal Mediterranean waters to warming, which are still poorly known, an in situ mesocosm experiment was carried out in Thau Lagoon during autumn 2018. Several microorganisms, including virio-, bacterio-, and phytoplankton < 10 µm in size, were monitored daily and analysed using flow cytometry for 19 consecutive days in six mesocosms. Three mesocosms (control) had the same natural water temperature as the lagoon, and the other three were warmed by + 3 °C in relation to the control temperature. The cytometric analyses revealed an unexpected community dominated by picophytoplanktonic cells, including Prochlorococcus-like and Picochlorum-like cells, which had not previously been... |
Tipo: Text |
Palavras-chave: Pico- and nanophytoplankton; Bacterioplankton; Virus; Warming; In situ mesocosm; Thau lagoon. |
Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00688/79979/82933.pdf |
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Bonami, Jean-robert. |
The trembling of legs followed by increasing paralysis and eventual death, were recorded on specimens of the above sp from Sete region. EM studies of tissue showed paraspherical bodies measuring approx 61 nm with a size range of 58-65 nm. By experimentally infecting specimens with a purified viral suspension the following pathogenesis was observed: in greater than 60 per cent of the specimens, trembling of the legs began within 6 days, growing very pronounced on the 8th day, leading to a general paralysis and 9 days after the infection a mortality rate of 70-80 per cent was recorded. The following characteristics of the virus are noted: its cytoplasmic localisation, paraspherical form, size ranging from 60-70 nm and the absence of an envelope. Considering... |
Tipo: Text |
Palavras-chave: Paralysis; Virus; Decapoda. |
Ano: 1973 |
URL: http://archimer.ifremer.fr/doc/1973/publication-2151.pdf |
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Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene. |
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using... |
Tipo: Text |
Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf |
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Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno. |
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés. |
Tipo: Text |
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf |
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Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J.. |
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (< 0.6 μ- and < 10 μ filtrates) were diluted either with < 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with < 30 kDa-benthic compounds (BC). The kinetics of heterotrophic... |
Tipo: Text |
Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf |
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Viscarra A,Tamara; Brebi M,Priscilla; Andana V,Alejandra; Sánchez G,Raúl. |
En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía de transmisión, debido principalmente a la afinidad de algunos patógenos con los espermatozoides. Dentro de los principales microorganismos infectantes en semen se encuentran Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Virus de la Inmunodeficiencia Humana... |
Tipo: Journal article |
Palavras-chave: Infecciones de transmisión sexual; Semen; Virus; Bacterias; Protozoos. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100041 |
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Registros recuperados: 115 | |
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