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| Registros recuperados: 992 | |
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| Wang,Limei; Wu,Xiuyin; Wang,Ruolin; Yang,Chengzhe; Li,Zhi; Wang,Cunwei; Zhang,Fenghe; Yang,Pishan. |
| Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western... |
| Tipo: Journal article |
Palavras-chave: Salivary adenoid cystic carcinoma; BRD4 inhibition; Proliferation; Migration; Epithelial-mesenchymal transition. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213 |
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| Dupré,Enrique M; Barros,Claudio. |
| This review compiles all the research done on gametes and fertilization in the rock shrimp, R. typus, and describes the sequence of events from the first gamete interaction to zygote formation and the first cleavage of the embryo, with light, fluorescence confocal and electron microscopes. Early studies showed that sperm from the vas deferens have a tack-shape with a "needle-like process" or rigid spike (RS) that extends from a semi-spherical body that contains the arms with chromatin and spines. Upon contact with seawater and by action of Na +, the arms and spines extend, producing an inverted umbrella form of the spermatozoa. The first sperm-oocyte interaction occurs between protein receptors type lectins of the sperm RS and oocyte chorion sperm ligands.... |
| Tipo: Journal article |
Palavras-chave: Spermatic ultrastructure; Oocyte; Sperm-egg interaction. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200003 |
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| Ontsouka,Edgar Corneille; Bertschi,Janique Sabina; Huang,Xiao; Lüthi,Michael; Müller,Stefan; Albrecht,Christiane. |
| BACKGROUND: Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMEC US) or Swiss Holstein-Friesian (bMEC CH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and α-smooth muscle actin (α-SMA.... |
| Tipo: Journal article |
Palavras-chave: Cell characterization; Flow cytometry; Gene expression; Lactation marker; Mammalian; Mammary epithelial cell. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100001 |
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| Moreno,Ricardo D; Laserre A,Andrea; Barros,Claudio. |
| The interaction between acrosome-reacted sperm and zona pellucida proteins is not yet fully understood. Serine protease acrosin and its zymogen proacrosin have been proposed to fulfill this function due to their capacity to bind zona pellucida glycoproteins. However, the molecular mechanism underlying this interaction has been merely speculative. Here we show that fucoidan (a sulfated polysaccharide) and solubilized zona pellucida glycoproteins, but not soybean trypsin inhibitor, are able to detach bound spermatozoa, which suggests that live sperm binds to the zona pellucida in a non-enzymatical way. Interestingly, mild proteolytic digestion with acrosin or trypsin does not modify the structure of the zona pellucida, but rather results in fewer spermatozoa... |
| Tipo: Journal article |
Palavras-chave: Fertilization; Acrosin; Acrosome; Zona pellucida; Sperm. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200006 |
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| VARELA,PATRICIA; TAPIA,GLADYS; FERNÁNDEZ,VIRGINIA; VIDELA,LUIS A. |
| Thyroid hormone (TH; 3,3',5-triiodothyronine, T3) is required for the normal function of most tissues, with major effects on 0(2) consumption and metabolic rate. These are due to transcriptional activation of respiratory genes through the interaction of T3-liganded TH receptors with TH response elements or the activation of intermediate factors, with the consequent higher production of reactive 0(2) species (ROS) and antioxidant depletion. T3-induced oxidative stress in the liver triggers the redox upregulation of the expression of cytokines (tumor necrosis factor-á [TNF-á], interleukin-10), enzymes (inducible nitric oxide synthase, manganese superoxide dismutase), and anti-apoptotic proteins (Bcl-2), via a cascade initiated by TNF-á produced by Kupffer... |
| Tipo: Journal article |
Palavras-chave: Thyroid hormone; Calorigenesis; Oxidative stress; Gene expression. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500004 |
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| Mamun,Md. Abdullah Al; Islam,Kamrul; Alam,Md. Jahangir; Khatun,Amina; Masihul Alam,M; Al-Bari,Md. Abdul Alim; Alam,Md. Jahangir. |
| BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive... |
| Tipo: Journal article |
Palavras-chave: Bone resorption; Osteoclast differentiation; Pit formation; TRAP positive cells. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051 |
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| Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea. |
| BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other... |
| Tipo: Journal article |
Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033 |
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| Mutua,Mbuvi P.; Muya,Shadrack; Gicheru,Muita M.. |
| In the mammalian lung, respiratory macrophages provide front line defense against invading pathogens and particulate matter. In birds, respiratory macrophages are known as free avian respiratory macrophages (FARM) and a dearth of the cells in the avian lung has been purported to foreordain a weak first line of pulmonary defense, a condition associated with high mortality of domestic birds occasioned by respiratory inflictions. Avian pulmonary mechanisms including a three tiered aerodynamic filtration system, tight epithelial junctions and an efficient mucociliary escalator system have been known to supplement FARM protective roles. Current studies, however, report FARM to exhibit an exceptionally efficient phagocytic capacity and are effective in... |
| Tipo: Journal article |
Palavras-chave: Avian lung FARM Phagocytosis PPAR γ Ligands. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100029 |
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| FIGUEROA,DANIELA; OLIVARES,RICARDO; SALABERRY,MICHEL; SABAT,PABLO; CANALS,MAURICIO. |
| We studied the lung diffusion parameters of two species of birds and two species of mammals to explore how structural and functional features may be paralleled by differences in life style or phylogenetic origin. We used two fast-flying species (one mammal and one bird), one running mammal and one bird species that flies only occasionally as models. The harmonic mean thickness of the air-blood barrier was very thin in the species we studied. An exception was the Chilean tinamou Notoprocta perdicaria, which only flies occasionally. It showed an air-blood barrier as thick as that of flightless Galliformes. We found that the respiratory surface density was significantly greater in flying species compared to running species. The estimated values for the oxygen... |
| Tipo: Journal article |
Palavras-chave: Diffusing capacity; Lungs; Flight. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200010 |
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| GÓMEZ,PATRICIA I; BARRIGA,ALEJANDRA; CIFUENTES,ANA SILVIA; GONZÁLEZ,MARIELA A.. |
| Dunaliella salina and D. bardawil are well-known microalgae accumulating high levels of b-carotene under growth-limiting conditions. In both taxa, this pigment is primarily composed of the isomers 9-cis and all-trans. The 9-cis b-carotene occurs only in natural sources and is the most attractive from a commercial point of view. The conditions that enhance the preferred accumulation of 9-cis b-carotene in D. salina are controversial and they have not been well established yet. This study examined the effect of salinity on the quantity and quality of total carotenoids and b-carotene isomers accumulated by D. salina (strain CONC-007) and D. bardawil (strain ATCC 30861) grown in two media with different nutritional compositions (PES and ART) and at salt... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200008 |
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| RECABARREN,SERGIO E; SIR-PETERMANN,TERESA; LOBOS,ALEJANDRO; CODNER,ETHEL; ROJAS-GARCÍA,PEDRO P; REYES,VÍCTOR. |
| Similar to women with Polycystic Ovary Syndrome (PCOS), female sheep treated prenatally with testosterone (T-females) are hypergonadotropic, exhibit neuroendocrine defects, multifollicular ovarian morphology, hyperinsulinemia and cycle defects. Hypergonadotropism and multifollicular morphology may in part be due to developmentally regulated increase in pituitary responsiveness to GnRH and may culminate in increased ovarian estradiol production. In this study, we utilized a GnRH agonist, leuprolide, to determine the developmental impact of prenatal testosterone exposure on pituitary-gonadal function and to establish if prenatal exposure produces changes in the reproductive axis similar to those described for women with PCOS. Eight control and eight... |
| Tipo: Journal article |
Palavras-chave: Androgenized females; Fetal programming; PCOS; Leuprolide; Female sheep. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200013 |
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| GARRIDO,ROGELIO; LAGOS,NÉSTOR; LATTES,KARINNA; GARCÍA,CARLOS; AZOLAS,RODRIGO; BOCIC,GUNTHER; CUNEO,ALDO; CHIONG,HECTOR; JENSEN,CRISTIAN; HENRÍQUEZ,ANA; FERNÁNDEZ,CRISTIAN. |
| The primary clinical symptom of Paralytic Shellfish Poisoning is acute paralytic illness produced by paralyzing toxins. Paralytic shellfish poison is formed by a mixture of phycotoxins and their toxicity is due to its reversible binding to a receptor site on the voltage-gated sodium channel on excitable cells, thus blocking neuronal transmission. We studied the effect of the gonyautoxin 2/3 epimers by local infiltration in the anal internal sphincter of healthy voluntary adults in order to reduce anal tone. The toxin was injected after prior clinical evaluation, anoscopy and anorectal manometry. Post injection clinical examination, electromyography and anorectal manometry were performed. Resting and voluntary contraction pressures were measured and the... |
| Tipo: Journal article |
Palavras-chave: Gonyautoxin 2/3; Phycotoxins; PSP toxins; Anal sphincter; Anal fissure. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300005 |
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| Registros recuperados: 992 | |
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