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| Registros recuperados: 9.251 | |
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| Crystian,Dennis; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero. |
| ABSTRACT: The aim of this study was to analyze the expression of putative reference genes in sugarcane under drought stress. The varieties RB72454 and RB72910 were cultivated and the treatments control and drought stress applied to 135-day-old plants grown under field conditions. The stress level of the plants was measured by rate of photosynthesis, transpiration, and stomatal conductance. For each biological replicate, expression analyses were conducted using quantitative polymerase chain reaction for the genes α-tubulin, β-tubulin, β-actin, cyclophilin, eukaryotic elongation factor 1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), histone H3 and ubiquitin. Stability was evaluated using the geNorm, NormFinder, and BestKeeper software packages. Among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Drought stress; Reference gen; Saccharum. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100203 |
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| Mota,Aquiles Paulino Peres; Dantas,João Carlos Pinheiro; Frota,Cristiane Cunha. |
| ABSTRACT: The rise in cases of antibiotic-resistant Mycobacterium tuberculosis has become a major obstacle to the effective control of tuberculosis (TB) worldwide. Essential oils (EO) are complex mixtures that may contain between 20 and 60 components, with two or three major compounds at relatively high concentrations (20-70%) that are responsible for their pharmacological properties. The objective of this study was to assess the antimicrobial activity of the EOs, bushy lippia (Lippia alba), rosemary pepper (Lippia sidoides), lemon grass (Cymbopogon citratus), Mexican mint or Indian borage (Plectranthus amboinicus), and true cinnamon (Cinnamomum zeylanicum), against Mycobacterium tuberculosis H37Rv. Chemical characterization of the EOs was performed by gas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anti-bacterial agent; Essential oils; Mycobacterium tuberculosis; Lippia alba; Lippia sidoides; Cymbopogon citrates; Plectranthus amboinicus; Cinnamomum zeylanicum.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600453 |
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| Santos,Esmael Lopes dos; Agassi,Victor José; Chicowski,Alessandro Sartor; Franchini,Julio Cezar; Debiasi,Henrique; Balbinot Junior,Alvadi Antonio. |
| ABSTRACT: In the last few cropping seasons, some soybean producers have tested the application of a hill drop sowing method, usually from three to four seeds every 30 to 40cm. However, there is a lack of information in the literature about the effect of this practice on soybean performance. Thus, the objective of this study was to evaluate the effect of a hill drop sowing with different number of plants per hole on soybean performance. The study was conducted in Londrina - PR, Brazil, during the 2013/14, 2014/15 and 2016/17 cropping seasons. The experiment was a randomized complete block design with four replications and a 2x5 factorial scheme. The first factor consisted of two soybean cultivars, BRS 359RR and NK 7059RR analyzed in the first two cropping... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max L.; Spatial plant arrangement; Spatial uniformity of plant distribution.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500201 |
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| Tafoya,Felipe Ayala; Juárez,Moisés Gilberto Yáñez; Orona,Carlos Alfonso López; López,Raymundo Medina; Alcaraz,Teresa de Jesús Velázquez; Valdés,Tomás Díaz. |
| ABSTRACT: Black shading nets are widely used in the protected cultivation of vegetables as a technique for controlling light and temperature, while the colored shading nets, with special optical properties to improve the use of solar radiation, appeared recently in the agricultural plastics market. Light quality transmitted by gray, aluminized, pearl, blue, red and black (control) nets with 30% shade was evaluated, as well as its effects on photosynthetic properties and fruits production of cucumber plants. Treatments (shade nets) were established under a randomized complete block design with four repetitions. The red net transmitted 23.7 and 40.3% more photosynthetic photon flux density (400 to 700nm) and red light (600 to 700nm) and the blue net... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis sativus L.; Light transmission; Photosynthetic responses; Leaf growth; Fruit production.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900200 |
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| Sugauara,Rosângela Rumi; Sugauara,Elisângela Yumi; Kazue Otutumi,Luciana; Germano,Ricardo de Melo; Sugauara,Elaine Yae Yamashita; Quessada,Ana Maria. |
| ABSTRACT: The aim of this paper was to conduct a bibliometry search about Brazilian scientific publications that describe experimental orthopedic surgeries in dogs. It was analyzed 14 publications, and there was wide variation in the number of experimental animals, showing that there is no consensus on the appropriate number for science experiment with dogs. Most articles had been reviewed by an ethics committee (13/14, 92.85%) and only two articles (2/14; 14.28%) did not report the use of analgesic protocol. Among analgesics, morphine was the most widely used, noted in seven studies (7/14; 42.85%). In six of the articles (6/14; 42.85%), the final destination of animals was not informed, but in four experiments (4/14; 28.57%) the dogs were euthanized. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Analgesia; Animal experimentation; Ethics committee; Surgical orthopedic.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000800601 |
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| Barili,Leiri Daiane; Vale,Naine Martins do; Silva,Fabyano Fonseca e; Carneiro,José Eustáquio de Souza; Oliveira,Hinayah Rojas de; Vianello,Rosana Pereira; Valdisser,Paula Arielle Mendes Ribeiro; Nascimento,Moyses. |
| ABSTRACT: We aimed to apply genomic information based on SNP (single nucleotide polymorphism) markers for the genetic evaluation of the traits “stay-green” (SG), plant architecture (PA), grain aspect (GA) and grain yield (GY) in common bean through Bayesian models. These models were compared in terms of prediction accuracy and ability for heritability estimation for each one of the mentioned traits. A total of 80 cultivars were genotyped for 377 SNP markers, whose effects were estimated by five different Bayesian models: Bayes A (BA), B (BB), C (BC), LASSO (BL) e Ridge regression (BRR). Although, prediction accuracies calculated by means of cross-validation have been similar within each trait, the BB model stood out for the trait SG, whereas the BRR was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; SNP markers; Cross-validation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000800204 |
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| Ziech,Rosangela Estel; Gressler,Leticia Trevisan; Frey,Joachim; Vargas,Agueda Castagna de. |
| ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiologic agent is the anaerobic bacterium Clostridium chauvoei. In recent years, the major virulence factors of C. chauvoei have been discovered and described. However, the pathogenesis of blackleg in cattle, and in particular, the movement of the pathogen from the point of entry to the affected tissues is not yet fully elucidated. Disease control is based on appropriate management and vaccination. This review summarizes the latest research findings that contribute toward the understanding of the disease in cattle, provide a foundation to preventive strategies, and identify future research needs. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium chauvoei; Sudden death; Myonecrosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500454 |
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| Gavilan,Natália Helena; Furlan,Fernanda Cardoso; Zorz,Alex Zichner; Oliveira,Leandro Silva de; Campos,Wellington Ferreira; Brondani,Gilvano Ebling. |
| ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autoclaving; Cerrado biome; Active chlorine; In vitro cultivation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900400 |
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| Witschinski,Fabiele; Demartini,Débora; Kilian,Josiane; Dallago,Rogério Marcos; Rosa,Clarissa Dalla; Cansian,Rogério Luis; Valduga,Eunice; Steffens,Juliana. |
| ABSTRACT: This study aimed to evaluate the effects of adding probiotic culture (Bifidobacterium animalis subsp. Lactis Bb-12) and prebiotics (fructooligosaccharide - FOS) to yoghurt formulations stored at 4°C for 28 days, using an experimental design (independent variables: (0-3% of FOS and probiotic starter cultures 0-3%). The pH, acidity, fat, syneresis, protein, ºBrix, sugars, FOS and probiotic bacteria count were analyzed. The probiotic- and prebiotic-added yoghurt formulations showed lower acidity, syneresis and glucose than the control yoghurt and compared to formulations containing probiotic and prebiotic separately. The 3% probiotic and prebiotic formulation showed a lower loss of concentration of FOS, and after 28 days presented 1.5g of FOS per... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yoghurt; Probiotic; Bifidobacterium; Prebiotics; Fructooligosaccharides. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300751 |
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| Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa. |
| ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451 |
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| Cunha,Tiago Garcia da; Visôtto,Liliane Evangelista; Lopes,Everaldo Antônio; Oliveira,Claúdio Marcelo Gonçalves; God,Pedro Ivo Vieira Good. |
| ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Esterase; Meloidogyne; Molecular biology; Morphological pattern. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200153 |
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| Lovio-Fragoso,Jose Pablo; Medina-Juárez,Luis Angel; Gamez-Meza,Nohemi; Martínez,Octavio; Hernández-Oñate,Miguel Ángel; Hayano-Kanashiro,Corina. |
| ABSTRACT: Plants belonging to genus Jatropha has arisen interest because of its high oil content that could be used to produce biodiesel. It is also widely reported that the main fatty acids in Jatropha oilseed are oleic and linoleic acids. However, there are scarce studies related to native species of Jatropha from Northwestern Mexico which are adapted to arid conditions, and the expression of genes involved in fatty acid synthesis for these species is still unknown. Therefore, the aim of this study was to analyze the expression of five genes, ACP1, KASII, D9SD, FAD2-1 and FAD2-2, which are involved in the oleic and linoleic acids synthesis in mature wild-seeds of Jatropha cinerea, a native species from Sonoran Desert, using semi-quantitative RT-PCR. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fatty acids biosynthesis; PCR; KASII; FAD2-1. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100405 |
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| Silverio,Gabriela Barros; Sakanaka,Lyssa Setsuko; Alvim,Izabela Dutra; Shirai,Marianne Ayumi; Grosso,Carlos Raimundo Ferreira. |
| ABSTRACT: Microencapsulation is used for protection and release of bioactive compounds. Combination of encapsulation methods allows the production of matrices with better technological properties compared to the application of one of the methods alone. Use of ionic gelation produces porous microparticles, and coating it with a protein, by electrostatic interaction, may contribute to a better protection of the active compound. The objective of the research was to produce alginate microparticles (AG) through ionic gelation and to coat them with soluble protein from soy protein concentrate. Two factors were studied, calcium concentration during ionic gelation (0.8, 1.6 and 2.4% w/w) and pH (3.5 and 7.0) of the protein solution for electrostatic interaction.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microencapsulation; Ionic gelation; Electrostatic interaction; Alginate; Soy protein. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200750 |
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| Iltchenco,Sidiane; Preci,Daiane; Bonifacino,Carla; Fraguas,Eugenia Franco; Steffens,Clarice; Panizzolo,Luis Alberto; Colet,Rosicler; Fernandes,Ilizandra Aparecida; Abirached,Cecilia; Valduga,Eunice; Steffens,Juliana. |
| ABSTRACT: This paper aim to evaluate the ultrafiltration (UF) process for constituents recovery from whey. Sequences of factorial designs were performed by varying temperature (5 to 40°C) and pressure (1 to 3 bar), to maximize the proteins concentration using membrane of 100kDa in dead end system. Based on the best result new experiments were performed with membrane of 50kDa and 10kDa. With the membrane of 50 the protein retention was about 3 times higher than the membrane of 100kDa. The concentrated obtained by UF membrane of 10kDa, 10°C and 2 bar in laboratory scale showed a mean protein retention of 80 %, greater protein solubility, emulsion stability and the identification of β-lactoglobulins (18.3 kDa) and α-lactalbumin fractions (14.2kDa). Therefore,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrafiltration; Whey; Dead-end solubility and emulsion stability. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500602 |
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| Santana,Jordana Almeida; Ferreira,Ana Carolina de Andrade; Souza,Marina de Castro Campos de; Moreira,Maria Aparecida Scatamburlo; Lima,Magna Coroa; Cruz,Diogo Soares Gonçalves; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. |
| ABSTRACT: Despite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens type D; Microbiota; Epsilon; Beta. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700452 |
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| Toledo,Zorayda; Simaluiza,Rosa Janneth; Fernández,Heriberto. |
| ABSTRACT: Occurrence and antimicrobial resistance profiles of Campylobacter jejuni and C. coli strains isolated from fecal samples of dogs, pigs, cows and hens in southern Ecuador were studied. Of the 250 samples studied, 84 (33.6%) were Campylobacter positive, with C. jejuni being more frequent (78.6%) than C. coli (21.4%), with the exception of porcine samples, from which C. coli was the only species isolated. Multidrug resistance was reported in 10 Campylobacter strains (11.9%), four C. jejuni and six C. coli, and was always associated with nalidixic acid and ciprofloxacin resistance. All of the amoxicillin-resistant strains were susceptible to amoxicillin-clavulanic acid and were therefore beta-lactamase producers. However, one strain of C. jejuni... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter; Reservoirs; Domestic animals; Antimicrobial resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100450 |
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| Registros recuperados: 9.251 | |
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