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| Registros recuperados: 992 | |
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| Yu,Guangchao; Chen,Lei; Lin,Chii-wann; Li,Bing; Cui,Hemiao; Chen,Siyi; Miao,Jian; Bian,Huawei; Chen,Dingqiang; Deng,Yang. |
| BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082... |
| Tipo: Journal article |
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Integron screening; Bacterial integrons; Class 1 integron; Class 2 integron; Class 3 integron. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100076 |
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| SAKURABA,SHIGEKI; TSUJITA,MIKI; ARISAKA,HIROFUMI; TAKEDA,JUNZO; YOSHIDA,KAZUICHI; KUWANA,SHUN-ICHI. |
| Buprenorphine is a mixed opioid receptor agonist-antagonist used in acute and chronic pain management. Although this agent's analgesic effect increases in a dose-dependent manner, buprenorphine-induced respiratory depression shows a marked ceiling effect at higher doses, which is considered to be an indicator of safety. Nevertheless, cases of overdose mortality or severe respiratory depression associated with buprenorphine use have been reported. Naloxone can reverse buprenorphine-induced respiratory depression, but is slow-acting and unstable, meaning that new drug candidates able to specifically antagonize buprenorphine-induced respiratory depression are needed in order to enable maximal analgesic effect without respiratory depression. Acetylcholine is... |
| Tipo: Journal article |
Palavras-chave: Buprenorphine; Donepezil; Rabbit; Respiratory depression; Cholinesterase inhibitor. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400008 |
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| LAMUELA-RAVENTÓS,ROSA M; GIMENO,EVA; FITÓ,MONTSE; CASTELLOTE,ANA-ISABEL; COVAS,MARIBEL; DE LA TORRE-BORONAT,M. CARMEN; LÓPEZ-SABATER,M. CARMEN. |
| Phenolic compounds have shown to inhibit LDL oxidation in vitro and ex vivo; however, they are hydrosoluble compounds while LDL is a lipoprotein. Analysis of phenolic compounds in LDLs by HPLC is necessary to demonstrate their binding capacity to lipoproteins. We developed and validated a solid phase extraction method (SPE) that allowed us the purification of LDL samples and their analysis by HPLC. This methodology allowed us to demonstrate the in vitro binding capacity of tyrosol, one of the main phenolic compounds in olive oil, to LDL. In the intervention dietary study with volunteers, food rich in phenolic compounds affected LDL composition. Changes in LDL phenolics composition are not observed after the short-term ingestion of food rich in phenolic... |
| Tipo: Journal article |
Palavras-chave: Antioxidant; LDL; Olive oil; Oxidation; Phenol; Tyrosol. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200011 |
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| PINCHEIRA,J; LÓPEZ,I; SANHUEZA,S; FERRUZ,P; NAVRRETE,MH; SANTOS,MJ; LÓPEZ-SÁEZ,JF. |
| The effect of the G2 repair of chromosomal damage in lymphocytes from workers exposed to low levels of X- or g-rays was evaluated. Samples of peripheral blood were collected from 15 radiation workers, 20 subjects working in radiodiagnostics, and 30 healthy control donors. Chromosomal aberrations (CA) were evaluated by scoring the presence of chromatid and isochromatid breaks, dicentric and ring chromosomes in lymphocytes with/without 5mM caffeine plus 3mM-aminobenzamide (3-AB) treatment during G2. Our results showed that the mean value of basal aberrations in lymphocytes from exposed workers was higher than in control cells (p< 0.001). The chromosomal damage in G2, detected with caffeine plus 3-AB treatment was higher than the basal damage... |
| Tipo: Journal article |
Palavras-chave: Chromosome aberrations ionizing radiation; G2 repair; Human lymphocytes; Occupational exposure. |
| Ano: 1999 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400009 |
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| Moreno,Adrian A; Orellana,Ariel. |
| Unfolded protein response (UPR) is a signaling mechanism activated by misfolded protein accumulation in the endoplasmic reticulum. It is a widespread process that has been described in organisms ranging from yeasts to mammals. In recent years, our understanding of UPR signaling pathway in plants has advanced. Two transcription factors from Arabidopsis thaliana have been reported to function as the sensor/ transducer of this response (AtbZIP60 and AtbZIP28). They seem to be involved in both heat and biotic stress. Furthermore, overexpression of one of them (AtbZIP60) produces plants with a higher tolerance for salt stress, suggesting that this transcription factor may play a role in abiotic stress. Furthermore, some data suggest that crosstalk between genes... |
| Tipo: Journal article |
Palavras-chave: Endoplasmic reticulum; Plant; Signaling; Unfolded protein response. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100010 |
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| RENAUD,SERGE; LANZMANN-PETITHORY,DOMINIQUE; GUEGUEN,RENÉ; CONARD,PASCALE. |
| A large number of prospective studies have observed an inverse relationship between a moderate intake of alcohol and coronary heart disease morbidity and mortality. Concerning death from all-causes, results are not unanimous. Alcohol intake was associated with a protection of all-cause mortality in England and USA physicians and the large study of the American Cancer Society. None of these studies separated the effects of different alcoholic beverages. In our prospective studies in France on 35 000 middle-aged men, we observed that only wine at moderate intake, was associated with a protective effect on all-cause mortality. The reason was that in addition to the known effect on cardiovascular diseases, a very moderate intake of wine, protected also from... |
| Tipo: Journal article |
Palavras-chave: Alcohol; Mortality; Wine. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200002 |
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| PATTERSON,SEAN I. |
| Protein domains play a fundamental role in the spatial and temporal organization of intracellular signaling systems. While protein phosphorylation has long been known to modify the interactions that underlie this organization, the dynamic cycling of lipids should now be included amongst the posttranslational processes determining specificity in signal transduction. The characteristics of this process are reminiscent of the properties of protein and lipid phosphorylation in determining compartmentalization through SH2 or PH domains. Recent studies have confirmed the functional importance of protein S-palmitoylation in the compartmentalization of signaling molecules that support normal physiological function in cell division and apoptosis, and synaptic... |
| Tipo: Journal article |
Palavras-chave: S-palmitoylation; Synapse; Growth cone; Regeneration; Phosphorylation. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200005 |
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| Rodríguez,Esteban M; Guerra,María M; Vío,Karin; González,César; Ortloff,Alexander; Bátiz,Luis F; Rodríguez,Sara; Jara,María C; Muñoz,Rosa I; Ortega,Eduardo; Jaque,Jaime; Guerra,Francisco; Sival,Deborah A; Dunnen,Wilfred F. A. den; Jiménez,Antonio J; Domínguez-Pinos,María D; Pérez-Fígares,José M; McAllister,James P; Johanson,Conrad. |
| Most cells of the developing mammalian brain derive from the ventricular (VZ) and the subventricular (SVZ) zones. The VZ is formed by the multipotent radial glia/neural stem cells (NSCs) while the SVZ harbors the rapidly proliferative neural precursor cells (NPCs). Evidence from human and animal models indicates that the common history of hydrocephalus and brain maldevelopment starts early in embryonic life with disruption of the VZ and SVZ. We propose that a "cell junction pathology" involving adherent and gap junctions is a final common outcome of a wide range of gene mutations resulting in proteins abnormally expressed by the VZ cells undergoing disruption. Disruption of the VZ during fetal development implies the loss of NSCs whereas VZ disruption... |
| Tipo: Journal article |
Palavras-chave: Hydrocephalus; Abnormal neurogenesis; Neural stem cells; Stem cell transplantation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300005 |
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| REYES,PAZ A; VARGAS,MACARENA F; GARCÍA,KATHERINE P; RUBILAR,PAULINA S; NAVARRETE,PATRICIA A; FUENTES,PAMELA M; RÍOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H; RUBIO,VÍCTOR H; HECKELS,JOHN; CHRISTODOULIDES,MYRON; CÁRDENAS,HUGO; VELASQUEZ,LUIS A. |
| Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of... |
| Tipo: Journal article |
Palavras-chave: Apoptosis; Fallopian tube; Neisseria gonorrhoeae; Salpingitis; TNF-alpha. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400006 |
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| Shu,Jun; He,Xiaojuan; Zhang,Lan; Li,Hong; Wang,Ping; Huang,Xiaojie. |
| BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the... |
| Tipo: Journal article |
Palavras-chave: Human amnion mesenchymal cells; THP-1 cells; TNF-ZZZZZZZ; IL-1XXXXXXXX; Immunosuppression. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069 |
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| Adonis,Marta I; Díaz,Jose; Miranda,Veronica R; Chahuan,Marco; Zambrano,Alcides; Benitez,Hugo C; Campos,Monica; Avaria,Pablo; Urzúa,Ulises; Marín,Pedro; Gohurdett,Mariela; Cisterna,Yasna; Gil,Lionel. |
| BACKGROUND: The mortality of lung cancer (LC), increases each year in the world, in spite of any advances, in development of new drugs to advance stages of LC. The high incidence of LC has been associated with smoking habit, genetic diversity and environmental pollution. Antofagasta region has been reported to have the highest LC mortality rate in Chile and its inhabitants were exposed to arsenic in their drinking water in concentrations as high as 870 μg/L. Non-invasive techniques such as biomarkers (Automatic Quantitative Cytometry: AQC and DR70) and Auto Fluorescence Bronchoscopy (AFB) might be potentially useful as a supplementary diagnostic approach and early detection. Early detection is one of the most important factors to intervene and... |
| Tipo: Journal article |
Palavras-chave: Early detection; Biomarkers; Lung cancer. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100056 |
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| Statti,Giancarlo A; Conforti,Filomena; Menichini,Federica; Marrelli,Mariangela; Gangale,Carmen; Tundis,Rosa; Loizzo,Monica R; Bonesi,Marco; Menichini,Francesco. |
| The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyperoside, isoquercetrin, quercitrin, quercetin and biapigenin) and chlorogenic acid of H. calabricum, have been determined. The ethyl acetate fraction from the aerial parts of H. calabricum exhibited activity against the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 1.6 jig/ml. The test for inhibition of nitric oxide (NO) production was performed using the murine monocytic macrophage cell line RAW 264.7. The ethyl acetate fraction had significant... |
| Tipo: Journal article |
Palavras-chave: Antiradical activity; Hypericaceae; Hypericum calabricum Sprengel; Phenolic compounds. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300001 |
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| Sukontason,Kabkaew L; Sanit,Sangob; Klong-klaew,Tunwadee; Tomberlin,Jeffery K; Sukontason,Kom. |
| BACKGROUND: Although tropical climate of Thailand is suitably endowed with biodiversity of insects, flies of medical importance is not well investigated. Using information from literature search, fly survey approach and specialist's experience, we review database of Sarcophaga (Liosarcophaga) dux Thomson (Diptera: Sarcophagidae), one of the priorities flesh fly species of medical importance in Thailand. RESULTS: This review deals with morphology, bionomics and medical involvement. Important morphological characteristics of egg, larva, puparia and adult were highlighted with illustration and/or micrographs. Search pertaining to molecular analysis used for fly identification and developmental rate of larvae were included. Medical involvement of larvae was... |
| Tipo: Journal article |
Palavras-chave: Sarcophaga dux; Review literature; Thailand; Forensic entomology; Myiasis; Morphology; Adult; Immature stages. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100014 |
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| Registros recuperados: 992 | |
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