SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides

Samain, Jean-francois.

French oyster farming has been subject to severe mortalities during the summer months. Results from the research program "Morest", which ran from 2000 to 2006 and examined the possible causes of these mortalities, led to the construction of a model to explain the interaction between environmental factors, oyster physiology and different opportunistic pathogens underlying oyster summer mortality. Temperature, food, reproduction and stress were the main factors required for oyster mortality. Genetically-based resistance ("R" oysters) or susceptibility ("S" oysters) to summer mortality was revealed by divergent selection. Building on these results, a literature search was made in 2007 on the molecular origin of genetic resistance to such a complex mortality...

Tipo: Text

Palavras-chave: Immunity; Antibacterial activity; Host defence; Environmental stress; Crassostrea gigas.

Ano: 2011

URL: http://archimer.ifremer.fr/doc/00051/16229/13752.pdf

Complex Relationships between the Blue Pigment Marennine and Marine Bacteria of the Genus Vibrio

Roch, Philippe; Yang, Y; Toubiana, Mylene; Aumelas, A.

Mytilin is a 34-residue antibacterial peptide from the mussel Mytilus galloprovincialis, which in addition possesses in vitro antiviral activity. The three-dimensional solution structure of the synthetic mytilin was established by using 1H NMR and consists of the common cysteine-stabilized alpha beta motif close to the one observed in the mussel defensin MGD-1. Mytilin is characterized by 8 cysteines engaged in four disulfide bonds (2-27, 6-29, 10-31, and 15-34) only involving the beta-strand II. Hydrophilic and hydrophobic areas of mytilin account for 63% and 37%, respectively, a ratio very close to that of MGD-1 (64% and 36%). One linear and three cyclic fragments were designed from the interstrand loop sequence known to retain the biological activities...

Tipo: Text

Palavras-chave: Shrimp; Molluscs; Mussel; Antibacterial activity; WSSV; Viral protection; Beta hairpin structure; NMR; Mytilin; Antimicrobial peptide.

Ano: 2008

URL: http://archimer.ifremer.fr/doc/2008/publication-4485.pdf

Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement

Falaise, Charlotte; James, Adele; Travers, Marie-agnes; Zanella, Marie; Badawi, Myriam; Mouget, Jean-luc.

Marennine, the water-soluble blue pigment produced by the marine diatom Haslea ostrearia, is known to display antibacterial activities. Previous studies have demonstrated a prophylactic effect of marennine on bivalve larvae challenged with a pathogenic Vibrio splendidus, suggesting that the blue Haslea is a good candidate for applications in aquaculture as a source of a natural antimicrobial agent. Indeed, the genus Vibrio is ubiquitous in aquaculture ecosystems, and regular events of pathogenic invasion cause some of the biggest losses worldwide. To better characterize the effects of marennine on Vibrios, a panel of 30 Vibrio strains belonging to 10 different species was tested, including bivalve pathogenic species (e.g., Vibrio crassostreae and Vibrio...

Tipo: Text

Palavras-chave: Antibacterial activity; Diauxie; Haslea; Hormesis; Marennine; Vibrio.

Ano: 2019

URL: https://archimer.ifremer.fr/doc/00484/59598/62602.pdf

Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed: Palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen Vibrio harveyi

Geiger, Marie.

The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...

Tipo: Text

Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf

Garcia-bueno, Nuria; Dumay, Justine; Guerin, Thomas; Turpin, Vincent; Paillard, Christine; Stiger-pouvreau, Valerie; Pouchus, Yves-francois; Aitor Marin-atucha, Arnaldo; Decottignies, Priscilla; Fleurence, Joel.

The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.

Tipo: Text

Palavras-chave: Antibacterial activity; Abalone disease; Haliotis tuberculata; Red seaweed; Vibrio harveyi.

Ano: 2015

URL: https://archimer.ifremer.fr/doc/00617/72875/71929.pdf

Antibacterial activity of propolis collected in different regions of Brazil

Gonsales,G. Z.; Orsi,R. O.; Fernandes Júnior,A.; Rodrigues,P.; Funari,S. R. C..

The aim of this study was to investigate the antibacterial activity of propolis samples from Goiás, Paraná and São Paulo States, Brazil, and their flavonoids content. Ethanolic extracts of propolis (EEP) were prepared (30g of propolis in 70% ethanol), and the microorganisms Staphylococcus aureus and Escherichia coli were tested. The methodology employed was agar diffusion using filter paper discs. Ampicillin and tetracycline were used as controls. Antibacterial activity was determined by the reading of inhibition zone diameters (mm) after 24 hours incubation at 37°C. Results demonstrated that EEP inhibited the growth of Staphylococcus aureus but not that of Escherichia coli. Tetracycline and ampicillin showed an efficient action against both bacteria....

Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase-positive strain

Palavras-chave: Propolis; Antibacterial activity; Flavonoids.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200009

Ratti,R. P.; Serrano,N. F. G.; Hokka,C. O.; Sousa,C. P..

Endophytic microorganisms are relatively unstudied as potential sources of novel natural products for medical and commercial exploitation. The aim of this work was to investigate some Brazilian tropical savannah trees Cassia leptophylla and Prunus spp. in order to isolate the endophytic microorganisms associated with these plants. The samples were disinfected to eliminate the epiphytic population. Colonies were diluted and displayed as drops in media and growing colonies were inactivated. Staphylococcus coagulase-positive strain was used as indicator microorganism and subjected to the antibioses test. Data showed that the microorganisms isolated from Cassia leptophylla had no inhibition against Staphylococcus. On the other hand, microorganisms isolated...

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Palavras-chave: Endophytic microorganisms; Plants; Antibacterial activity; Bioactive compounds.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200007

Barbosa,PSF; Martins,AMC; Toyama,MH; Joazeiro,PP; Beriam,LOS; Fonteles,MC; Monteiro,HSA.

Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis...

Purification and antibacterial activities of an L-amino acid oxidase from king cobra (Ophiophagus hannah) venom

Palavras-chave: Bothrops moojeni; Kidney; Platelet aggregation; Insulin; Antibacterial activity.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016

Phua,CS; Vejayan,J; Ambu,S; Ponnudurai,G; Gorajana,A.

Some constituents of snake venom have been found to display a variety of biological activities. The antibacterial property of snake venom, in particular, has gathered increasing scientific interest due to antibiotic resistance. In the present study, king cobra venom was screened against three strains of Staphylococcus aureus [including methicillin-resistant Staphylococcus aureus (MRSA)], three other species of gram-positive bacteria and six gram-negative bacteria. King cobra venom was active against all the 12 bacteria tested, and was most effective against Staphylococcus spp. (S. aureus and S. epidermidis). Subsequently, an antibacterial protein from king cobra venom was purified by gel filtration, anion exchange and heparin chromatography. Mass...

Chemical composition and antibacterial activity of Brazilian propolis essential oil

Palavras-chave: L-amino acid oxidase; King cobra; Antibacterial activity; Ophiophagus hannah.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200010

Oliveira,AP; França,HS; Kuster,RM; Teixeira,LA; Rocha,LM.

The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). In addition, the agar diffusion method using filter paper disks was employed. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37º C for 24 hours. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. New components, namely...

Antibacterial activity of Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) (Hemiptera: reduviidae) venomS against human pathogens

Palavras-chave: Apis mellifera; Propolis essential oil; Terpenoids; Antibacterial activity.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100012

Sahayaraj,K.; Borgio,J. F.; Muthukumar,S.; Anandh,G. P..

The reduviid predators Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) use their venoms to paralyze their preys. We detected the antibacterial activity of R. marginatus and C. brevipennis venoms against seven Gram-negative and four Gram-positive bacteria by using the disc diffusion method. Rhynocoris marginatus venom exhibited antibacterial activity against four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium) and one Gram-positive (Streptococcus pyogenes). Catamirus brevipennis venom showed antibacterial activity against six Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium) and three...