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| Registros recuperados: 796 | |
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| Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir, H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle. |
| Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size... |
| Tipo: Text |
Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf |
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| Muller-feuga, Arnaud. |
| Industrial potentialities related to biotechnology of microalgae are examined, highlighting a wide spectrum of applications which concern principally nutrition, health, environment and energy. The french situation of research on microalgae, especially at Ifremer, is assessed, showing that the main axis are fine chemistry, aquaculture, environment, bio-process engineering. It covers the majority of the applications, but leaves in shade certain important aspects such as human food and CO2 fixation. Concerning the general approach, some significant improvements can be expected from a more accurate control of bacteriological and genetic quality of cultures. About the industrial aspects, a national initiative seems in progress. The variety of potential... |
| Tipo: Text |
Palavras-chave: Process engineering; Energy; Environment; Health; Food; Aquaculture; Biotechnology; Microalgae; Génie des procédés; Energie; Environnement; Santé; Alimentation; Aquaculture; Biotechnologie; Microalgues. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/rapport-1092.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf |
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| Cadoret, Jean-paul; Bardor, M; Lerouge, P; Cabigliera, M; Henriquez, Vitalia; Carlier, Aude. |
| Extraction of natural substances and chemical synthesis are the main sources of pharmaceutical molecules. When possible, one may transfer the gene of the molecule in living cells creating individual factories producing on demand and in a safe way the requested molecule. Today, bacteria, yeast, mammalian cells and plants constitute the main plate-forms for various commercial products. Microalgae present numerous advantages and could offer a powerful tool for the production of commercial molecules in a near future. |
| Tipo: Text |
Palavras-chave: Genetics; Recombinant; Algae; Pharmaceuticals; Gene transfer; Biotechnology; Génétique; Recombinant; Algues; Pharmaceutique; Transfert de gène; Biotechnologie. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4045.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf |
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| Rumin, Judith; Nicolau, Elodie; Junior, Raimundo Gonçalves De Oliveira; Fuentes-grünewald, Claudio; Picot, Laurent. |
| A bibliographic database of scientific papers published by authors affiliated to research institutions worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to characterize the research trends related to microalgae markets, markets opportunities and technologies that could have important impacts on markets evolution. Six major markets opportunities, the production of biofuels, bioplastics, biofertilizers, nutraceuticals, pharmaceuticals and cosmetics, and two fast-evolving technological domains driving markets evolution, microalgae harvesting and extraction technologies... |
| Tipo: Text |
Palavras-chave: Bibliometric; Microalgae; Biofertilizers; Bioplastics; Biotechnology; Cosmetics; Europe; Food; GMO; Market; Nutraceuticals; Pharmaceuticals; Research. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74185/73788.pdf |
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| Petr Lokhov. |
| Immunization with cancer cells is of great demand in anti-cancer therapy. However, current cellular vaccines are inefficient and there are questions regarding their overall safety. We report a simple and straightforward approach for improving of cellular cancer vaccines. Through treatment of cancer cell cultures with purified protease, it is possible to make preparations of cell-surface antigens that are free of intracellular content and contain two orders-of-magnitude less protein than the whole lysate of an equivalent number of cancer cells. Despite this difference in total protein content, protease-generated preparations stimulate anti-cancer responses from immune cells better those stimulated with cancer cells themselves. The composition of collected... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Cancer; Pharmacology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4356/version/1 |
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| Dong Xiao; Enyuan Wang. |
| Microbially Enhance Coalbed Methane technology is used to improve methane content in coalbeds. The methanogen consortium metabolism process is the basic avenue for MECoM to yield methane with some organic compounds in coal. Detailed information about the metabolism process is needed in studying the microbial consortium mechanism. Traditional gas analysis is limited by gas source and test complexity. The real time monitor with Infrared gas sensor breaks the gas analysis limitation. It shows much more detailed information than the traditional analysis method including information about microbial growth factor changes, reaction speeds for environmental changes, the detailed relationship between CH4 and CO2, methane yield and the consumption factor by real... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Microbiology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5426/version/1 |
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| Huilei Xu; Avi Ma'ayan. |
| Results from multivariate molecular biological experiments become increasingly complex. Hence, the challenge of projecting high-dimensional data onto few dimensions for effective data visualization is becoming increasingly important in Systems Biology. Effective data visualization can summarize the activity of many variables over time as well as display relationships between variables. Dynamic interactive visualization tools can provide scientists with ways of visually identifying relationship and patterns, and improve communication of results on the web and in presentations. For this, interactive systems with animation have great potential since they add dimensions to static images limited to two dimensions. Interactivity and animation is particularly... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3074/version/1 |
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| Babajan B; Chaitanya M; Anuradha CM; Suresh Kumar Chitta. |
| The emergence of tuberculosis resistant to multiple, first- and second-line antibiotics poses challenges to a global control strategy that relies on standard drug treatment regimens. The high drug-resistant strains of Mycobacterium tuberculosis (Mtb) have been implicated in outbreaks and have been found throughout the world; a comprehensive understanding, the magnitude of this threat requires an accurate assessment of the worldwide burden of resistance. In an attempt to design anti-TB drugs, the target chosen is a key enzyme of Mtb, O-Succinylbenzoate synthase (OSBS), which is an attractive target for its role in electron transport chain as OSBS is not available in humans. An attempt has been to built the 3-D structure of Mtb-OSBS using online Swiss model... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Microbiology; Pharmacology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3776/version/1 |
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| Elibio L. Rech. |
| The Amazon basin is an area of mega-biodiversity. Different models have been proposed^1-8^ for the establishment of an effective conservation policy, increasing sustainability and adding value for biodiversity. Currently, a broad spectrum of technologies from genomics to synthetic biology is available, and these permit the collection, manipulation and effective evaluation of countless organisms, metabolic pathways and molecules that exist as potential products of a large, biodiverse ecosystem. The use of Genomics and synthetic biology may constitute an important tool and be a viable option for the prospection, evaluation and manipulation of biodiversity as advocated as well as be useful for developing methods for sustainable use and the production of novel... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Genetics & Genomics; Earth & Environment. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/5759/version/1 |
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| Etienne Joly. |
| To obtain an optimised form of BFP for use as a reporter gene in mammalian cells, the brightest available GFP form, EGFP, was mutated at 5 different positions, yielding 8 different mutagenised forms of BFP. The intensity of the fluorescent signals attained in mammalian cells with all these various versions of BFP was analysed by flow cytometry of transiently transfected COS 7 cells. The best mutant obtained can be detected readily both by flow cytometry and fluorescence microscopy, even when expressed together with GFP. To explore whether cellular localization could enhance the fluorescence signals any further, plasmid constructs were made to target optimised versions of GFP and BFP to the nucleus, the endoplasmic reticulum (ER) and the cell surface.... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1259/version/1 |
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| Katja Neubauer; Jenny Bandomir; Anett Grunenberg; Andreas Müller; Udo Kragl; Kerstin Schmidt. |
| With the environmental risk assessment (ERA) for genetically modified plants (GMP) the identification of equivalence or differences in the composition of ingredients of GMPs and non-GMPs has to be investigated. The strategy is based upon the idea that traditionally cultivated crops have been used safely for many years, thus every divergence within the compounds of a GMP in contrast to its non-GMP counterpart could refer to a risk and has to be further analysed.

The BioOK company offers the opportunity to analyse very different aspects from the ERA of GMPs in one hand. Beside toxicological analysis and the determination of environmental effects via soil analysis, we can look into several plant metabolites to identify... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Chemistry; Plant Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4439/version/2 |
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| Tiefeng You; I. C. Baianu. |
| The soybean-derived products are among the most important agricultural products in the USA and the world. Conventional analytical methods for soybean composition analysis are both time consuming and costly. Faster and less expensive methods are required for most practical applications. To improve the accuracy, reliability and sensitivity of NIR, major advancements in instrumentation, as well as, data analysis / calibration methodology are required. Novel NIR instruments, such as DA-NIR and FT-NIR spectrometers developed in recent years have the potential for improving significantly the quantification of soybean composition at a reasonable cost. We present representative calibrations and data for intact soybean composition analysis obtained at the... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6253/version/1 |
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| Stuart L. Moodie; Nicolas Le Novere; Emek Demir; Huaiyu Mi; Falk Schreiber. |
| Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialized notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Bioinformatics. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/3721/version/3 |
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| Vinita Yadav; Kapil Deo Singh Yadav. |
| Two fungal strains were isolated and tentatively identified as Penicillium VY and Aspergillus VY. All the isolated species show the maximum production on third day in a liquid culture media. The pH optimum was found to be 10.0 for Penicillium VY and 11.0 for Aspergillus VY. The temperature optima were 50ºC in both the cases. The enzyme produced by Penicillium VY was found to be stable in the pH range 3.0-7.0 and 3.0–6.0 in case of Aspergillus VY. The enzyme does not loose activity up to 40º C in case of Penicillium VY and 40ºC in case of Aspergillus VY if exposed for 1 h.
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| Tipo: Poster |
Palavras-chave: Biotechnology; Chemistry; Microbiology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2560/version/1 |
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| Gudmundur A. Thorisson; Mikael Linden; Anthony J. Brookes; Myles Byrne; Juha Muilu; Tommi Nyronen. |
| Motivation for the IRISC workshop came from the observation that identity and digital identification are increasingly important factors in modern scientific research, especially with the now near-ubiquitous use of the Internet as a global medium for dissemination and debate of scientific knowledge and data, and as a platform for scientific collaborations and large-scale e-science activities.

The 1 1/2 day IRISC2011 workshop sought to explore a series of interrelated topics under two main themes: i) unambiguously identifying authors/creators & attributing their scholarly works, and ii) individual identification and access management in the context of identity federations. Specific aims of the workshop... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Data Standards. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6609/version/1 |
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| Registros recuperados: 796 | |
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