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Registros recuperados: 293 | |
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Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P.. |
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006 |
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Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge. |
The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017 |
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Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann. |
Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H S = 0.52). Cluster analysis and principal component based scatter plotting... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015 |
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Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry. |
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067 |
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Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli. |
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010 |
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Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del. |
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010 |
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Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.. |
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014 |
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Mousinho-Ribeiro,Rita de Cassia; Pante-de-Sousa,Gabriella; Santos,Eduardo José Melo dos; Guerreiro,João Farias. |
The distribution of b-globin gene haplotypes was studied in 209 Amerindians from eight tribes of the Brazilian Amazon: Asurini from Xingú, Awá-Guajá, Parakanã, Urubú-Kaapór, Zoé, Kayapó (Xikrin from the Bacajá village), Katuena, and Tiriyó. Nine different haplotypes were found, two of which (n. 11 and 13) had not been previously identified in Brazilian indigenous populations. Haplotype 2 (+ - - - -) was the most common in all groups studied, with frequencies varying from 70% to 100%, followed by haplotype 6 (- + + - +), with frequencies between 7% and 18%. The frequency distribution of the b-globin gene haplotypes in the eighteen Brazilian Amerindian populations studied to date is characterized by a reduced number of haplotypes (average of 3.5) and low... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA polymorphisms; Brazilian Amerindians; Genetic diversity. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300002 |
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Wei,Shuhong; Peng,Zhengsong; Zhou,Yonghong; Yang,Zaijun; Wu,Kai; Ouyang,Zhongming. |
In this work, we examined the genetic diversity and evolution of the WAG-2 gene based on new WAG-2 alleles isolated from wheat and its relatives. Only single nucleotide polymorphisms (SNP) and no insertions and deletions (indels) were found in exon sequences of WAG-2 from different species. More SNPs and indels occurred in introns than in exons. For exons, exons+introns and introns, the nucleotide polymorphism Π decreased from diploid and tetraploid genotypes to hexaploid genotypes. This finding indicated that the diversity of WAG-2 in diploids was greater than in hexaploids because of the strong selection pressure on the latter. All dn/ds ratios were < 1.0, indicating that WAG-2 belongs to a conserved gene affected by negative selection. Thirty-nine of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Copy number variation; Genetic diversity; Molecular evolution; Triticum; WAG-2. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400013 |
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Ghafouri-Kesbi,Farhad. |
The aim of this study was to monitor changes in genetic size of a small-closed population of Iranian Zandi sheep, by using pedigree information from animals born between 1991 and 2005. The genetic size was assessed by using measures based on the probability of identity-by-descend of genes (coancestry, f, and effective population size, Ne), as well as measures based on probability of gene origin (effective number of founders, f e, effective number of founder genomes, f g, and effective number of non-founder genomes, f ne). Average coancestry, or the degree of genetic similarity of individuals, increased from 0.81% to 1.44% during the period 1993 to 2005, at the same time that Ne decreased from 263 to 93. The observed trend for f e was irregular throughout... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pedigree; Effective size; Genetic drift; Genetic diversity; Genetic similarity; Sheep. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400011 |
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Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland. |
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016 |
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Favoretto,Patrícia; Veasey,Elizabeth Ann; Melo,Paulo César Tavares de. |
The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Germplasm banks; Genetic diversity; Microsatellites. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017 |
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Sudré,Cláudia P; Leonardecz,Eduardo; Rodrigues,Rosana; Amaral Júnior,Antônio T do; Moura,Maria da CL; Gonçalves,Leandro SA. |
The research on plant genetic resources is essential for the conservation of genetic diversity and accessions' divergence studies, as a basis for plant breeding. Aiming to know the state of art in this subject, a historical survey was carried out in Revista de Olericultura and in Horticultura Brasileira, from 1961 to 2006, searching for papers dealing with vegetable crops genetic resources. In each of the papers, the species studied, first author institution, publication year, applied software, number of accessions and descriptors, and the multivariate techniques used were registered. Based on these characteristics, papers were grouped using multivariate analysis. Sixty-one papers dealt somehow with genetic resources in the time covered by the survey, from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Review; Multivariate analysis; Diagnosis; Genetic diversity; Vegetables. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000400002 |
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Weber,Laura I.; Hildebrand,Cintia G.; Ferreira,Anderson; Pedarassi,Gustavo; Levy,José A.; Colares,Elton P.. |
A genetic study of the neotropical river otter Lontra longicaudis (Olfers, 1818), which has an unknown conservation status, was carried out at the Taim Ecological Station and the margins of the Vargas stream, Rio Grande do Sul, southern Brazil. Faecal samples were collected, and DNA was extracted using a silica-guanidine method. Five microsatellite loci were amplified using PCR with heterologous primers previously described for Lutra lutra (Linnaeus, 1758). Sixteen faecal samples out of 29 from Taim and 11 out of 14 from Vargas stream margins contained enough DNA for genetic analysis. A total of 49 different alleles were found at both localities, from which 18 were exclusively found in individuals from Taim and 17 were exclusives from Vargas individuals.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neotropical river otter; Genetic markers; Taim; Non-invasive sampling; Genetic diversity. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212009000100001 |
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Registros recuperados: 293 | |
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