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Registros recuperados: 100 | |
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Ribeiro,M.G.; Lara,G.H.B.; Bicudo,S.D.; Souza,A.V.G.; Salerno,T.; Siqueira,A.K.; Geraldo,J.S.. |
Relata-se, pela primeira vez no Brasil, a ocorrência de mastite gangrenosa caprina atípica causada pela co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli em uma cabra da raça Boer, na segunda semana de lactação. Descrevem-se os achados clínicos, os procedimentos de diagnóstico microbiológico e a conduta terapêutica. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Gangrenous mastitis; Etiology; Co-infection. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300037 |
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Nóbrega Jr.,J.E.; Rossetto,R.; Matos,M.H.T.; Chaves,R.N.; Magalhães,D.M.; Lima-Verde,I.B.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.; Gonçalves,P.B.D.; Oliveira,J.F.C.. |
This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P<0.05) in the percentage of primordial follicles in all groups... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preservation; Preantral follicles; Sphingosine 1-phosphate; Follicular activation. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000400977 |
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Rodrigues,G.Q.; Lima,I.M.T.; Chaves,R.N.; Rossetto,R.; Costa,S.L.; Castro,S.V.; Barros,V.R.P.; Matos,M.H.T.; Lopes,C.A.P.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicle; In vitro development; TGF-β. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200013 |
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Bandeira,D.A.; Castro,R.S.; Azevedo,E.O.; Nascimento,E.R.; Melo,L.S.S.; Melo,C.B.. |
O objetivo deste trabalho foi verificar a ocorrência de Mycoplasma agalactiae, com o uso da reação em cadeia da polimerase (PCR), em 30 rebanhos caprinos leiteiros nas microrregiões do Cariri Ocidental e Oriental do estado da Paraíba. Das 120 amostras de leite testadas, nove (7,5%) apresentaram amplificação de apenas um fragmento com 360pb. As amostras que amplificaram este fragmento estavam presentes em 6/30 (20%) fazendas localizadas nos municípios de Amparo, Santo André e Gurjão. |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Goat; Mycoplasmosis; Milk; CASG. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500031 |
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Fonseca,J.F.; Esteves,L.V.; Zambrini,F.N.; Brandão,F.Z.; Peixoto,M.G.C.D.; Verneque,R.S.; Siqueira,L.G.B.; Viana,J.H.M.. |
O objetivo do presente estudo foi avaliar a viabilidade da técnica de transferência não cirúrgica em cabras. Quatro cabras não-lactantes pluríparas da raça Toggenburg foram utilizadas como receptoras de embriões, sendo que duas receberam um embriões e duas receberam dois embriões coletados não cirurgicamente cabras doadoras. Os corpos lúteos das receptoras foram detectados um dia antes da transferência de embriões por ultrassonografia transretal. Uma seringa de 5mL contendo 2mL de meio holding foi acoplada em um cateter tomcat, no qual os embriões foram aspirados em uma coluna central a duas outras colunas. Um espéculo Colin número 2 foi inserido na vulva e na vagina, e com o uso de uma fonte de luz, a cerviz foi localizada e imobilizada com uma pinça de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Non-surgical embryo transfer; Cervical via. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200040 |
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Fonseca,J.F.; Batista,R.I.T.P.; Souza-Fabjan,J.M.G.; Oliveira,M.E.F.; Brandão,F.Z.; Viana,J.H.M.. |
ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo survival; Embryo transfer; Goat; Slow freezing. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501489 |
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Wang,Y.; Qin,X.; Chen,Y.; Xing,J.; Gao,J.; Qiu,D.. |
RESUMO O objetivo do presente estudo foi investigar a soroprevalência de infecção com Toxoplasma gondii (T. gondii) em cabras em cinco províncias do noroeste da China. Soroprevalência foi determinado usando o kit de teste de ensaio de imunoabsorção enzimática (ELISA). A soroprevalência geral foi 21.23% (197/928). Análise estatística revlou que diferenças significativas foram observadas em fêmeas (P= 0.048, OR= 0.567, 95% CI= 0.309 a 1.041) e nos grupos ≥ 2 (P= 0.002, OR= 0.330, 95% CI= 0.224 a 0.488). Nenhuma diferença estatisticamente significativa foi observada entre diferentes províncias. Nossos resultados indicam que a infecção com T. gondii, que pode ter implicações importantes sobre a saúde pública, teve diferenças significativas em sexo e idade, mas... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Toxoplasma gondii; Seroprevalence; Goat; Five northwestern provinces. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100277 |
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Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth. |
Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003 |
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Moura,Raylene Ramos; Melo,Luciana Magalhães; Freitas,Vicente José de Figueirêdo. |
Among all the transgenic mammalians produced so far, goats have represented an excellent model of transgenesis when considering the factors such as the market demand for protein, volume of milk produced per lactation and reproductive rate. Various recombinant proteins have been obtained from the transgenic and non-transgenic goats, and among these, human antithrombin, produced by the transgenic goats, was the first recombinant protein of animal origin to be released as a drug for the clinical use in humans. This review reports the aspects inherent to the production of recombinant proteins in the goats, from the production of the animal bioreactors up to the expression of these proteins in their milk. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenesis; Goat; Recombinant protein; Milk. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500010 |
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Costa,Sonia Helena Furtado; Santos,Regiane Rodrigues dos; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Rodrigues,Ana Paula Ribeiro; Ohashi,Otávio Mitio; Figueiredo,José Ricardo de. |
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Goat; Preantral follicles; Coconut water; Saline solution. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000600009 |
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SANTOS,Renato de Lima; BARRETO FILHO,João Bosco; MARQUES Jr.,Antônio de Pinho; ANDRADE,Joelmer Santos. |
The goal of this research was to determine the volumetric proportions of the structural components of the goat placenta throughout gestation. We used nine goats of nondefined breed, separated into groups A, B and C, with 90, 120 and 150 days of gestation, respectively. From each animal fragments of three placentomes were obtained, one removed from the base, another from the middle part and the other from the end of the gestational uterine horn. The fragments were fixed in Bouin solution and histologically processed according to the standard techniques and stained with hematoxilin-eosin. Microscopically, a 25-point circular grid (Zeiss KPL ocular 6.3x with 25 points) was randomly used across the placentome to determine the volumetric proportion of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Placenta; Morphology; Goat. |
Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000400002 |
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Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R.. |
The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GDF-9; IGF-I; GH; Culture; Follicle activation; Goat. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006 |
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Han,Xufeng; Wang,Lei; Li,Wei; Li,Bibo; Yang,Yuxin; Yan,Hailong; Qu,Lei; Chen,Yulin. |
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Green fluorescent protein; Gastro-intestinal tract; Lactobacillus plantarum; Goat. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849 |
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Silva,José Roberto Viana; Brasil,Alline Ferreira; Santos,Regiane Rodrigues dos; Costa,Sônia Helena Furtado; Rodrigues,Ana Paula Ribeiro; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Figueiredo,José Ricardo de. |
The present work has investigated the degeneration rate of goat primordial follicles in situ after preservation in PBS or TCM 199 at different temperatures and incubation times. For each animal the ovarian pair was divided into 19 fragments. One ovarian fragment was taken randomly and immediately fixed (control). The other 18 ovarian fragments were randomly distributed in tubes containing PBS or TCM 199 and stored at 4º, 20º or 39ºC for 4, 12 or 24h. The storage of ovarian fragments in PBS or TCM 199 at 20ºC for 12h and 24h or at 39ºC, in all incubation times tested, increased significantly the percentage of degenerated primordial follicles (P<0.05). In contrast, for both media tested the degeneration rate of primordial follicles preserved at 4ºC for up... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Primordial follicles; Degeneration; Goat; Preservation. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500019 |
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Menezes,Erika Bezerra de; Salles,Maria Gorete Flores; Silva,Cleidson Manoel Gomes da; Fernandes,César Carneiro Linhares; Galeati,Giovanna; Araújo,Airton Alencar de; Rondina,Davide. |
ABSTRACT: The effect of insulin administration on the productive responses of Saanen goats during early lactation was investigated. Ten of 20 adult females were subjected to subcutaneous administration of intermediate-acting insulin (0.14UI/kg body weight) at 2, 9, and 14 days postpartum. Milk yield was measured twice daily for 13 weeks and milk samples were collected to measure protein and fat contents. Plasma levels of progesterone, insulin, non-esterifies fatty acids, glucose and other metabolites were measured. Results showed a significantly increased effect of insulin treatment on the content of milk fat and protein; moreover, milk production in the first and second postpartum weeks were higher than control group. The peak of lactation in the insulin... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Insulin; Milk; Postpartum; Metabolites. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300652 |
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Registros recuperados: 100 | |
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