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| Registros recuperados: 181 | |
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| Galindo,Bruno A.; Ferreira,Dhiego G.; Apolinário-Silva,Caroline; Terra,Mariana C.; Aprígio,Nícollas G. O.; Ota,Renata R.; Ohara,Willian M.; Almeida,Fernanda S.; Sofia,Silvia H.. |
| ABSTRACT Brycon nattereri is an endangered Neotropical fish reported along conserved stretches of the upper Paraná, Tocantins and São Francisco rivers. Populations of this species have been very rare in some Paraná River sub basins. This study analyzes the genetic diversity and population structure of B. nattereri in a restricted area of occurrence recently identified in upper Paraná River basin. Seven microsatellite loci and 497 bp of D-Loop mitochondrial region were examined in 92 individuals from four points along the area of occurrence. Both molecular markers indicated a single population distributed along a stretch of the river approximately 80 km long. Although some of the data suggest an ancient bottleneck, current levels of genetic diversity (H E... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Endangered species; Molecular markers; Population genetics; Pirapitinga. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100208 |
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| Vaini,Jussara Oliveira; Grisolia,Alexéia Barufatti; Prado,Fernanda Dotti do; Porto-Foresti,Fábio. |
| Hybrids of the species Pseudoplatystoma corruscansvs.Pseudoplatystoma reticulatum are extensively traded because they are more docile and precocious compared to their parents. This study aimed to identify the occurrence of hybrid 'Surubim' in rivers of the State of Mato Grosso do Sul, applying molecular techniques. Pseudoplatystoma corruscans, Pseudoplatystoma reticulatum and hybrid 'Surubim' were detected using multiplex PCR and PCR-RFLP in RAG2, GLOBIN, EF1α, 18S rRNA nuclear genes, and mitochondrial 16S rRNA gene. The results revealed the presence of F1 hybrids in rivers and the occurrence of genetic introgression, evidenced by the Post-F1 hybrids. Furthermore, discrepancies between the morphological and genetic identification were observed,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Fish; Hybridization; Introgression; Molecular markers. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300635 |
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| Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Kelly,Lucy; Bermejo,Juan Vicente Delgado. |
| The objective of this work was to evaluate the genetic diversity of Brazilian Pantaneiro horse by microsatellite markers, investigate the effect of genetic bottlenecks and estimate genetic differentiation among four horse breeds. Genetic variation was estimated through allele frequencies and mean breed heterozygosity. Nei's genetic distances among the breeds Pantaneiro, Thoroughbred, Arabian, Spanish Pure Breed (Andalusian), and Uruguay Creole were calculated, and it was used to construct an UPGMA dendrogram. Clustering at different K values was calculated to infer population structure and assign individuals to populations. Nei's distances showed a minimum distance between Pantaneiro horse and Spanish Pure Breed (0.228), and similar distances from Spanish... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equus caballus; Animal conservation; Molecular markers; Microsatellites; Genetic resources conservation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500007 |
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| Ferreira,Marcia Flores da Silva; Cervigni,Gerardo Domingo Lucio; Ferreira,Adésio; Schuster,Ivan; Santana,Fernanda Abreu; Pereira,Waldir Dias; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
| The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Breeding; Marker-assisted selection; Molecular markers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400012 |
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| Bruel,Daniela Cristina; Carpentieri-Pípolo,Valéria; Gerage,Antonio Carlos; Fonseca Júnior,Nelson da Silva; Prete,Cássio Egídio Cavenaghi; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Souza,Silvia Graciele Hülse de; Garbuglio,Deoclécio Domingos. |
| The objective of this work was to evaluate the genetic diversity of 16 maize inbred lines, and to determine the correlation between genetic distance and hybrid performance, using random amplified polymorphic DNA (RAPD) molecular markers. Twenty-two different random primers were used, which resulted in the amplification of 265 fragments, 237 (84.44%) of them being polymorphic. A genetic similarity matrix was created from the RAPD data, using Jaccard coefficient, and a dendrogram was constructed. Hybrid analyses were carried out using random block design and Griffing method VI for diallel crossings. The genetic associations showed five distinct heterotic groups. Correlations between genetic divergences detected by RAPD, as well as the means observed in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Diallel; Heterotic patterns; Molecular markers. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001000006 |
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| Cabral,Pablo Diego Silva; Soares,Taís Cristina Bastos; Gonçalves,Leandro Simões Azeredo; Amaral Júnior,Antonio Teixeira do; Lima,Andreia Barcelos Passos; Rodrigues,Rosana; Matta,Frederico de Pina. |
| The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Genetic diversity; Germplasm characterization; Joint analysis; Molecular markers; Morphological markers. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000011 |
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| Pinheiro,Letícia da Silveira; Rangel,Paulo Hideo Nakano; Vianello,Rosana Pereira; Brondani,Claudio. |
| The objective of this work was to determine the effect of male sterility or manual recombination on genetic variability of rice recurrent selection populations. The populations CNA-IRAT 4, with a gene for male sterility, and CNA 12, which was manually recombined, were evaluated. Genetic variability among selection cycles was estimated using14 simple sequence repeat (SSR) markers. A total of 926 plants were analyzed, including ten genitors and 180 individuals from each of the evaluated cycles (1, 2 and 5) of the population CNA-IRAT 4, and 16 genitors and 180 individuals from each of the cycles (1 and 2) of CNA 12. The analysis allowed the identification of alleles not present among the genitors for both populations, in all cycles, especially for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Allele variation; Rice breeding; Molecular markers; Population improvement. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600011 |
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| Fonseca,Kenia Gracielle da; Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Barth,Mariana; Feldberg,Nelson Pires. |
| Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora; Molecular markers; Multivariate analysis; Plant cultivar protection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000849 |
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| Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry. |
| The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019 |
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| Paiva,Samuel Rezende; Silvério,Vanessa Chaves; Egito,Andréa Alves; McManus,Concepta; Faria,Danielle Assis de; Mariante,Arthur da Silva; Castro,Sílvia Ribeiro; Albuquerque,Maria do Socorro Maués; Dergam,Jorge Abdala. |
| The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo (48), Somali (48), Morada Nova (48) and Bergamasca (46), collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aries; Conservation genetics; Domestic animal diversity; Molecular markers; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900008 |
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| Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da. |
| The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008 |
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| Silva,Gabi Nunes; Nascimento,Moysés; Sant’Anna,Isabela de Castro; Cruz,Cosme Damião; Caixeta,Eveline Teixeira; Carneiro,Pedro Crescêncio Souza; Rosado,Renato Domiciano Silva; Pestana,Kátia Nogueira; Almeida,Dênia Pires de; Oliveira,Marciane da Silva. |
| Abstract: The objective of this work was to evaluate the use of artificial neural networks in comparison with Bayesian generalized linear regression to predict leaf rust resistance in Arabica coffee (Coffea arabica). This study used 245 individuals of a F2 population derived from the self-fertilization of the F1 H511-1 hybrid, resulting from a crossing between the susceptible cultivar Catuaí Amarelo IAC 64 (UFV 2148-57) and the resistant parent Híbrido de Timor (UFV 443-03). The 245 individuals were genotyped with 137 markers. Artificial neural networks and Bayesian generalized linear regression analyses were performed. The artificial neural networks were able to identify four important markers belonging to linkage groups that have been recently mapped,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Hemileia vastatrix; Artificial intelligence; Molecular markers; Prediction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000300186 |
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| Soares,Taís Cristina Bastos; Good-God,Pedro Ivo Vieira; Miranda,Fábio Demolinari de; Soares,Janaína Bastos; Schuster,Ivan; Piovesan,Newton Deniz; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
| The objectives of this study were to detect quantitative trait loci (QTL) for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL), obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively). Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Linkage map; Molecular markers; Recombinant inbred lines; Quantitative trait loci. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100012 |
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| Sayd,Ricardo Meneses; Amabile,Renato Fernando; Faleiro,Fabio Gelape; Bellon,Graciele. |
| The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hordeum vulgare; Genetic diversity; Molecular markers; Quantitative traits.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200160 |
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| Registros recuperados: 181 | |
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