SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Silva Feliciano,Cinara; Rodrigues Plaça,Jessica; Peronni,Kamila; Araújo Silva Jr,Wilson; Roberto Bollela,Valdes.

Abstract Tuberculosis (TB) is still considered a major global public health problem in the world and there is a concern about the worldwide increase of drug-resistance (DR). This paper describes the analysis of three Mycobacterium tuberculosis isolates from a single patient collected over a long treatment period of time. DR was initially investigated through phenotypic testing, followed by line probe assays (LPAs) and whole genome sequencing (WGS). It presents an intriguing situation where a multidrug-resistant (MDR-) TB case was diagnosed and treated based only on late phenotypic drug susceptibility testing of isolate 1. During the treatment, another two isolates were cultivated: isolate 2, nine months after starting MDR-TB treatment; and isolate 3,...

Research on tuberculosis: 75 years of IBIT contributions

Palavras-chave: Tuberculosis; Drug resistant tuberculosis; Drug susceptibility tests; Whole genome sequencing.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300012

Leite,Geraldo.

In 1937, the foundation year of the Brazilian Institute for Tuberculosis Research, three questions persisted in the researchers' mind: "What is the real value of BCG? How to solve the problem of the chest register of large communities? When will we have a specific drug for the treatment of tuberculosis?"; Along with this line of thought, the author presents a list of the Institute's main contributions, by pointing out the following topics: different diagnoses between pulmonary tuberculosis and pulmonary schistosomiasis; innocuity of iodine salts in pulmonary tuberculosis; relative value of Weltmann and Takata-Ara's reactions, and of blood and lipase rates in bacillosis; the value of the bacteriologic diagnosis (method of precipitation, standardization of...

Physician adherence to guidelines for tuberculosis and HIV care in Rio de Janeiro, Brazil

Palavras-chave: Research; Tuberculosis; Bahia; Brazil.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200014

Saraceni,Valeria; Pacheco,Antonio Guilherme; Golub,Jonathan E; Vellozo,Vitoria; King,Bonnie S; Cavalcante,Solange C; Eldred,Lois; Chaisson,Richard E; Durovni,Betina.

BACKGROUND: Tuberculosis is the most common opportunistic infection among HIV-infected patients in Brazil. Brazil's national policy for HIV care recommends screening for latent tuberculosis (TB) and implementing isoniazid preventive therapy (IPT). OBJECTIVES: We compared physician adherence to TB screening and other prevention and care policies among HIV primary care clinics in Rio de Janeiro City. METHODS: Data on performance of CD4 counts, viral load testing, tuberculin skin testing (TST) and IPT were abstracted from patient charts at 29 HIV clinics in Rio de Janeiro as part of the TB/HIV in Rio (THRio) study. Data on use of pneumocystis jiroveci pneumonia (PCP) prophylaxis were also abstracted from a convenience sample of 150 patient charts at 10 HIV...

Immune reconstitution syndrome in patients treated for HIV and tuberculosis in Rio de Janeiro

Palavras-chave: AIDS-related opportunistic infections; Tuberculosis; Disease prevention; Guideline adherence.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300012

Serra,Fabiana C.; Hadad,David; Orofino,Renata L.; Marinho,Flavia; Lourenço,Cristina; Morgado,Mariza; Rolla,Valeria.

We made a retrospective longitudinal study from January 2000 to January 2003 to examine cases of immune reconstitution syndrome (IRS) and its incidence rate in tuberculosis (TB)-human immunodeficiency virus (HIV) co-infected patients. The incidence rate (IR) was calculated using a Poisson regression. The confidence interval (CI) that was stipulated was 95%. IRS occurred in 10/84 HIV and TB-positive patients; nine of them were on highly active anti-retroviral therapy (HAART) during a mean of 61.7 (±59) days following the introduction of antiretrovirals. Lymph-node enlargement was the sole clinical manifestation. CD4 counts were <100 cells/mm³in 50% of the patients, at the time of TB diagnosis. All but two patients were treated with prednisone, and...

Concentrations of rifampicin in pre-dose samples in patients with pulmonary tuberculosis

Palavras-chave: AIDS; Immune reconstitution syndrome; Tuberculosis; HAART; Lymph node enlargement; Paradoxical reaction.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500004

Uchoa,Breno Kristoffer Beleza; Albério,Carlos Augusto Abreu; Pinto,Ana Carla Godinho; Lucena,Stefania de Medeiros Araujo; Vieira,José Luiz Fernandes.

ABSTRACT Rifampicin is used in both phases of treatment for tuberculosis. In chronic use, the short half-life and the self-induction of metabolism can decrease the levels of the drug below the minimal inhibitory concentration. The aim of the study was to investigate whether plasma concentrations of rifampicin are sustained above 0.5 µg/mL in patients with tuberculosis using 600 mg/day. Rifampicin was measured in plasma by high-performance liquid chromatography and a sputum smear microscopy was performed in all days of the study. A total of 44 male patients completed the study. On days 31, 61 and 91, the mean plasma concentrations of rifampicin were 0.6 (0.5) µg/mL, 0.55 (0.5) µg/mL and 0.46 (0.4) µg/mL. There was a high variation of rifampicin levels...

The NRAMPI, VDR and TNF-α gene polymorphisms in Iranian tuberculosis patients: the study on host susceptibility

Palavras-chave: Infectious disease; Tuberculosis; Rifampicin.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200130

Merza,Muayad; Farnia,Parissa; Anoosheh,Sabar; Varahram,Mohammed; Kazampour,Mehdi; Pajand,Omid; Saeif,Shima; Mirsaeidi,Mehdi; Masjedi,Mohammad Reza; Velayati,Ali Akbar; Hoffner,Sven.

The natural resistance-associated macrophage protein (NRAMP1), Vitamin-D receptor (VDR) and Tumor necrosis factor (TNF-α) have been associated in susceptibility to tuberculosis, but the results have been inconsistent. This study aimed to determine the association of NRAMP1, VDR, and TNF-á variant with development of pulmonary tuberculosis (PTB) among Iranian patients. The single nucleotide polymorphisms (SNPs) at INT4, D543, 3'UTR of NRAMP1 gene, SNPs in restriction sites of BsmI, and FokI of the VDR gene and SNPs of TNF-α at -238,-308, -244,857,-863 positions were analyzed by PCR-RFLP among two groups of individual; patients with PTB (n=117) and healthy controls (n=60). Thereafter, the frequencies of extended haplotypes and diplotypes were estimated. No...

Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil

Palavras-chave: NRAMP; VDR; TNF-α gene; Tuberculosis.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400002

Luiz,Roberta dos Santos Silva; Suffys,Phillip; Barroso,Elizabeth Clara; Kerr,Ligia Regina Franco Sansigolo; Duarte,Cynthia Romariz; Freitas,Max Victor Carioca; Mota,Rosa Maria Salani; Frota,Cristiane Cunha.

OBJECTIVES: This study has used a combination of clinical information, spoligotyping, and georeferencing system to elucidate the genetic diversity of the Mycobacterium tuberculosis isolates circulating in a TB-prevalent municipality of Northeast Brazil. METHODS: A total of 115 M. tuberculosis strains were isolated from pulmonary tuberculosis patients from January 2007 to March 2008 in Fortaleza. Drug susceptibility and spoligotyping assays were performed and place of residence of the patients were georeferenced. RESULTS: Of the M. tuberculosis strains studied, 51 (44.3%) isolates were resistant to at least one drug (R-TB) and 64 (55.7%) were sensitive to all the drugs tested (S-TB). A high frequency of resistance was found in previously treated cases (84%)...

Assessment of the effectiveness of a home-based care program for patients coinfected with tuberculosis and human immunodeficiency virus after discharge from a reference hospital in South-Eastern Brazil

Palavras-chave: Tuberculosis; Drug-resistant tuberculosis; Georeferencing; Spoligotyping.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300006

Bento,Carlos Alessandro Plá; Pedroso,Enio Roberto Pietra.

The effectiveness of tuberculosis treatment delivered by a home-based care program to patients coinfected with HIV was compared with that of a service provided by outpatient departments. A retrospective study was made of a cohort of coinfected patients discharged from hospital between January 1998 and December 2002 who had been followed-up for one year within one of these programs. Two-hundred-and-forty-three patients who met the inclusion criteria were grouped according to their treatment program (group 1 received home-based care; group 2 attended outpatient departments) and then paired one-to-one across the groups by gender, age and level of education. Only 48 patients from each group could be paired. Apart from the duration of HIV infection, there were...

Chronic symptoms and pulmonary dysfunction in post-tuberculosis Brazilian patients

Palavras-chave: AIDS; Tuberculosis; Treatment outcome; Adherence to treatment; Home-based care.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600008

Nihues,Simone de Sousa Elias; Mancuzo,Eliane Viana; Sulmonetti,Nara; Sacchi,Flávia Patussi Correia; Viana,Vanessa de Souza; Martins Netto,Eduardo; Miranda,Silvana Spindola; Croda,Julio.

ABSTRACTBACKGROUND: Questionnaire and spirometry were applied to post-tuberculosis indigenous and non-indigenous individuals from Dourados, Brazil, to investigate the prevalence of chronic respiratory symptoms and pulmonary dysfunction.METHODS:This was a cross-sectional study in cured tuberculosis individuals as reported in the National System on Reportable Diseases (SINAN) from 2002 to 2012.RESULTS:One hundred and twenty individuals were included in the study and the prevalence of chronic respiratory symptoms was 45% (95% CI, 34-59%). Respiratory symptoms included cough (28%), sputum (23%), wheezing (22%) and dyspnea (8%). These symptoms were associated with alcoholism, AOR: 3.1 (1.2-8.4); less than 4 years of schooling, AOR: 5.0 (1.4-17.7); and previous...

Challenges in the development of drugs for the treatment of tuberculosis

Palavras-chave: Brazil; Dysfunction; Spirometry; Tuberculosis.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500492

Shehzad,Adeeb; Rehman,Gauhar; Ul-Islam,Mazhar; Khattak,Waleed Ahmad; Lee,Young Sup.

Tuberculosis infection is a serious human health threat and the early 21st century has seen a remarkable increase in global tuberculosis activity. The pathogen responsible for tuberculosis is Mycobacterium tuberculosis, which adopts diverse strategies in order to survive in a variety of host lesions. These survival mechanisms make the pathogen resistant to currently available drugs, a major contributing factor in the failure to control the spread of tuberculosis. Multiple drugs are available for clinical use and several potential compounds are being screened, synthesized, or evaluated in preclinical or clinical studies. Lasting and effective achievements in the development of anti-tuberculosis drugs will depend largely on the proper understanding of the...

HLA profile in patients with AIDS and tuberculosis

Palavras-chave: Antibiotics; Drug resistance; Tuberculosis; Pathogen.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100012

Figueiredo,José Fernando de Castro; Rodrigues,Maria de Lourdes Veronese; Deghaide,Neifi Hassam Saloum; Donadi,Eduardo Antonio.

Studies carried out in various populations have reported an association between some HLA specificities and susceptibility to tuberculosis. We investigated the class I and class II HLA profile in Brazilian patients of various ethnic backgrounds who had AIDS and tuberculosis. Twenty-two adult patients with AIDS and tuberculosis (Group I), 103 patients with AIDS without tuberculosis (Group II) and 423 healthy individuals not infected with HIV (Group III) were evaluated. Diagnosis of HIV infection was made by ELISA, confirmed by a gelatin particle agglutination test. Diagnosis of tuberculosis was made based on clinical/radiological presentation and direct bacilloscopy or clinical specimen cultures. Class I antigens were typed by microlymphotoxicity. Class II...

Localized hepatic tuberculosis presenting as fever of unknown origin

Palavras-chave: AIDS; Tuberculosis; Host genetic factors; HLA.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400004

Ferrari,Teresa Cristina Abreu; Couto,Carolina Mundim; Vilaça,Tatiane Silva; Xavier,Marcelo Antônio Pascoal.

Localized hepatic tuberculosis is a rare clinical form of tuberculosis infection; it has signs and symptoms related only to hepatic injury, with minimal or no extrahepatic involvement. It frequently presents as a non-specific syndrome, with systemic manifestations, which can sometimes result in a diagnostic dilemma. A high index of suspicion is required and a definitive diagnosis can be very difficult. We report a case of localized hepatic tuberculosis that presented as fever of unknown origin.

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Tuberculosis; Fever of unknown origin; Granulomatous hepatitis.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500013

Pulmonary Alveolar Proteinosis and tuberculosis in a diabetic patient: a rare or a seldom diagnosed association

Pereira-Silva,J.L.; Marinho,M.M.M.A.; Veloso,T.V.B.; Coelho Filho,J.C..

A case of Pulmonary Alveolar Proteinosis (PAP), in association with tuberculosis, is described in a 35-year-old diabetic patient. Lung biopsy showed an intra-alveolar accumulation of PAS-positive material, and multifocal granulomas compatible with tuberculosis. The bronchoalveolar culture was positive for Mycobacterium tuberculosis. PAP results from an imbalance of the mechanisms that regulate the homeostasis of the surfactant, where specific proteins are involved, especially SP-A and SP-D, the cytokines, IL-10 and GM-CSF, in addition to alveolar macrophages and type-II pneumocytes. Chemotaxis and phagocytic capacity are reduced. PAP and diabetes share several immunological disfunctions that may increase the risk for tuberculosis. Although there are no...

Tuberculosis and AIDS co-morbidity in Brazil: linkage of the tuberculosis and AIDS databases

Palavras-chave: Pulmonary Alveolar Proteinosis (PAP); Tuberculosis; Diabetic patient.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400006

Miranda,Angelica Espinosa; Golub,Jonathan E.; Lucena,Francisca de Fátima; Maciel,Ethel Noia; Gurgel,Maria de Fátima; Dietze,Reynaldo.

This study evaluated differences in AIDS patients with and without tuberculosis (TB) in Espírito Santo State, Brazil. Standard regional AIDS (SINAN, SISCEL, SICLOM and SIM) and tuberculosis (SINAN) databases were used. TB and AIDS databases were linked using Reclink software, version 3, with SPSS software support to identify co-infected cases. Data from July 2000 to June 2006 in Espírito Santo State were linked. The results showed 3,523 adult AIDS cases and 9,958 adult TB cases resulted in 430 co-infected patients, who were compared to 1,290 AIDS patients who never developed TB. Among 430 co-infected patients, TB was diagnosed first in 223 (51.9%), AIDS was first in 44 (10.2%), and AIDS and TB were diagnosed concurrently in 163 (37.9%). Median age did not...

A study of iga antibody response to different mycobacterium tuberculosis antigens in the diagnosis and monitoring of pulmonary tuberculosis

Palavras-chave: AIDS; Tuberculosis; Co-infection; Secondary data.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200013

Bezerra,Janaina Miranda; Beck,Sandra Trevisan; Kanunfre,Kelly Aparecida; Leite,Olavo Munhoz; Ferreira,Antônio Walter.

We evaluated the performance of the ELISA technique in the detection of IgA antibodies against different Mycobacterium tuberculosis antigenic preparations in serum samples from 49 patients with pulmonary tuberculosis collected before and after the start of specific treatment. The controls consisted of serum samples from healthy patients without any prior contact with the bacteria and serum samples from patients with other pneumopathies. Glycolipid antigen gave the best diagnostic performance, with a sensitivity of 88% and specificities varying from 88 to 100% in the control groups. These antigens constitute a powerful tool for the diagnosis and monitoring of patients with pulmonary tuberculosis.

Measurement of peripheral blood mononuclear cells producing IFN-Gamma in patients with tuberculosis

Palavras-chave: Tuberculosis; IgA; Serological diagnosis; ELISA; Glycolipid antigen.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100012

Andrade Júnior,Dahir Ramos de; Santos,Sânia Alves dos; Andrade,Dahir Ramos de.

The type of immune response induced by tuberculosis (Th1 or Th2) and its correlation with the clinical outcome is unclear. We studied 13 patients with active tuberculosis (TBC). The peripheral blood mononuclear cells producing IFN-gamma (PBMC-IG) were measured by enzyme-linked immunospot (ELISPOT) technique. The control group had ten healthy individuals vaccinated against tuberculosis. We collected blood samples of each patient in two moments: a) in the hospital admission without treatment (TBC1); b) after seven to 20 days of treatment (TBC2). The comparison of the spots forming units of PBMC-IG between TBC group and controls showed that there was a significant difference between TBC1 and control group (p < 0.001) and between TBC2 and control group (p...

Tuberculosis treatment outcome and associated factors among smear-positive pulmonary tuberculosis patients in Afar, Eastern Ethiopia: a retrospective study

Palavras-chave: Tuberculosis; Th1 immune response; ELISPOT.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200005

Zenebe,Tizazu; Tefera,Ermias.

ABSTRACT Evaluating the outcomes of tuberculosis treatment and understanding the specific reasons for unfavorable treatment outcome are important in evaluating the effectiveness of tuberculosis control program. A retrospective study was conducted to assess tuberculosis treatment outcomes and associated factors among smear positive pulmonary tuberculosis patients in zone-one health facilities of Afar regional state, Ethiopia. A total of 380 smear positive pulmonary tuberculosis patients’ registration book recorded with complete information from Jan 2011 to Dec 2013 were analyzed. Of 380 patients included in the analysis, 238 were male and 142 female with mean age of 30.7. Overall treatment outcome were 128 (33.7%) cured, 192 (50.2%) completed, 17 (4.5%)...

Association of genetic polymorphisms of CISH with the risk of pulmonary tuberculosis in Zahedan, Southeast Iran

Palavras-chave: Tuberculosis; Treatment outcome; Smear positive pulmonary TB; Ethiopia.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600635

Naderi,Mohammad; Hashemi,Mohammad; Safdari,Abolhassan; Bahari,Gholamreza; Taheri,Mohsen.

Abstract Background In the current study we aimed to find out the impact of cytokine-inducible Src homology 2 domain protein (CISH) gene polymorphisms on the risk of pulmonary tuberculosis (PTB) in a sample of Iranian population. Materials and methods Polymorphisms of CISH rs2239751, rs414171, and rs6768300 were determined in 200 PTB patients and 200 healthy subjects using T-ARMS-PCR or PCR-RFLP method. Results The results showed that rs414171 A>T genotypes significantly decreased the risk of PTB (OR = 0.16, 95% CI = 0.10–0.27, p < 0.0001, AT vs AA; OR = 0.31, 95% CI = 0.14–0.68, p < 0.0001, TT vs AA; OR = 0.19, 95% CI = 0.12–0.29, p < 0.0001, AT+TT vs AA; OR = 0.29, 95%CI = 0.20–0.42, p < 0.0001, T vs A). For rs6768300, the findings...

High incidence of tuberculosis in patients treated for hepatitis C chronic infection

Palavras-chave: Tuberculosis; CISH; Polymorphism.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400379

de Oliveira Uehara,Silvia Naomi; Emori,Christini Takemi; Perez,Renata Mello; Mendes-Correa,Maria Cassia Jacintho; de Souza Paiva Ferreira,Adalgisa; de Castro Amaral Feldner,Ana Cristina; Silva,Antonio Eduardo Benedito; Filho,Roberto José Carvalho; de Souza e Silva,Ivonete Sandra; Ferraz,Maria Lucia Cardoso Gomes.

Abstract Brazil is one of the 22 countries that concentrates 80% of global tuberculosis cases concomitantly to a large number of hepatitis C carriers and some epidemiological risk scenarios are coincident for both diseases. We analyzed tuberculosis cases that occurred during α-interferon-based therapy for hepatitis C in reference centers in Brazil between 2001 and 2012 and reviewed their medical records. Eighteen tuberculosis cases were observed in patients submitted to hepatitis C α-interferon-based therapy. All patients were human immunodeficiency virus-negative. Nine patients (50%) had extra-pulmonary tuberculosis; 15 (83%) showed significant liver fibrosis. Hepatitis C treatment was discontinued in 12 patients (67%) due to tuberculosis reactivation and...

Risk of tuberculosis among household contacts in Salvador, Bahia

Palavras-chave: Hepatitis C; Tuberculosis; Alpha-interferon; Latent tuberculosis infection.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200205

Lemos,Antonio Carlos; Matos,Eliana D; Pedral-Sampaio,Diana B; Netto,Eduardo M..

Tuberculosis is one of the most important infectious diseases in the world. Only 68% of the estimated new tuberculosis (TB) cases in Brazil are diagnosed. Our aim was to determine the risk of infection among household contacts. Study design. Cohort of tuberculin-negative household contacts followed for 12 Months. Methods. Household contacts of randomly selected index acid-fast bacilli (AFB)-positive TB cases were evaluated through clinical examination, thorax X-ray, tuberculin, AFB smear and culture. Contacts with a negative response to the tuberculin test (less than 10 mm diameter) were retested after 90 days. Tuberculin reversal (used as a parameter of infection risk) was defined as an increase of at least 10 mm from the last measurement. Results. 269...