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Metabolism of CO and CH sub(4) by nitrifiers and the determination of the nitrification rate. ArchiMer
Morita, R; Jones, R.
The nitrifying bacteria were found to survive 24 weeks in the absence of ammonium without decreasing their number of cell size. Because H sub(2), CO, and CH sub(4) are present in the marine environment, these substrates were investigated as a possible source of the energy of maintenance for the nitrifying bacteria. super(14)CO and super(14)CH sub(4) were found to be oxidized by the nitrifiers. N-serve was found to inhibit the oxidation of CO. Using the nitrifiers' ability to oxide CO, a method for the determination of the nitrification rate was developed. The ability of nitrifiers to oxidize CO may play a significant role in the cycling of CO sub(2) in the marine environment. Whether CO and CH sub(4) oxidation play a role in the survival of nitrifiers in...
Tipo: Text Palavras-chave: Methane; Carbon dioxide; Survival; Bacteria; Nitrification; Nitrogen cycle; Oxidation; Carbon cycle; Biogeochemistry; Metabolism.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-975.pdf
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Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria. ArchiMer
Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf
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Kinetics of tidal resuspension of microbiota: Testing the effects of sediment cohesiveness and bioturbation using flume experiments ArchiMer
Blanchard, Gérard F; Sauriau, Pg; Cariou-le Gall, Valerie; Gouleau, D; Garet, Mj; Olivier, F.
Resuspension of the top few sediment layers of tidal mud flats is known to enhance planktonic biomass of microbiota (benthic diatoms and bacteria). This process is mainly controlled by tidal shear stress and cohesiveness of mud, and is also influenced by bioturbation activities. Laboratory experiments in a race track flume were performed to test the interactive effects of these factors on both the critical entrainment and resuspension kinetics of microbiota from silt-clay sediments from the Marennes-Oleron Bay, France. The marine snail Hydrobia ulvae was used to mimic surface bioturbation activities. As expected, the kinetics of microbial resuspension versus shear stress were largely controlled by the cohesiveness of silt-clay sediments. However, our...
Tipo: Text Palavras-chave: Microphytobenthos; Bacteria; Resuspension; Bioturbation; Hydrobia ulvae; Shear velocity; Sediment cohesiveness; Flume experiment.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00337/44789/44480.pdf
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Evolution de la communaute bacterienne heterotrophe de l'eau de mer lors d'une experience d'aquaculture de crevettes peneides en systemes clos ArchiMer
Sohier, L; Bianchi, M.
The quantitative and qualitative evolution of the heterotrophic bacterial community of sea water was studied throughout an experimental rearing of Penaeus japonicus in closed systems. Cluster analysis was realised on 417 strains coming from water sampled at different moments of the experiment. This collection includes 40 strains coming from a mussel used for diet and 40 strains coming from the digestive tract of one prawn after six months rearing. Quantitative and qualitative studies have both shown a similar evolution of heterotrophic microflora whatever the initial animal load was. The dendrogram obtained led to a description of characteristics of the various populations constituting the heterotrophic bacterial community of the water environment...
Tipo: Text Palavras-chave: Penaeus japonicus; Malacostraca; Bacteria; Recirculating systems; Numerical taxonomy; Temporal variations; Microorganisms; Shrimp culture; Heterotrophic organisms.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-972.pdf
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Desulfurobacterium atlanticum sp nov., Desulfurobacterium pacificum sp nov and Thermovibrio guaymasensis sp nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria ArchiMer
L' Haridon, S; Reysenbach, A.l.; Tindall, B. J.; Schonheit, P; Banta, A; Johnsen, U; Schumann, P; Gambacorta, A; Stackebrandt, E; Jeanthon, C.
Three thermophilic, anaerobic, strictly chemolithoautotrophic, sulphur- and/or thiosulphate-reducing bacteria, designated SL17(T), SL19(T) and SL22(T), were isolated from deep-sea hydrothermal samples collected at 13 degrees N (East Pacific Rise), Guaymas Basin (Gulf of California) and 23 degrees N (Mid-Atlantic Ridge), respectively. These strains differed in their morphology, temperature range and optimum for growth, energy substrates and 16S rRNA gene sequences. The G + C content of the genomic DNA was 41 mol% (SL22(T)), 42 mol% (SL17(T)) and 46 mol% (SL19(T)). Comparative analysis of phenotypic and phylogenetic traits indicated that strains SL17(T) and SL22(T) represented two novel species of the genus Desulfurobacterium and that strain SL19(T) should...
Tipo: Text Palavras-chave: Hydrothermal environment; Phylogeny; Thermophiles; Bacteria.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2174.pdf
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Etude des liens trophiques au sein d'une communaute bacterienne selectionnee a partir d'un sediment marin sur le cyclohexene-4 dicarboximide-1,2 (THPI) ArchiMer
Terrom, G; Gil, G; Lepetit, J.
A bacterial community has been selected from a marine sediment on 4-cyclohexene-1,2 dicarboximide or THPI (fungicide). This community consists of nine strains. The "principal" strain, Photobacterium mandapamensis , degrades THPI by different metabolic pathways many of which require co-oxidative sequences. The other eight "auxiliary" strains occur at different states along the trophic chain formed by the "principal" strain, so increasing velocity and intensity of the THPI biodegradation.
Tipo: Text Palavras-chave: Bacteria; Marine pollution; Microorganisms; Metabolism; Fungicides; Biodegradation; Sediment pollution; Trophic relationships.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1021.pdf
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Effects of petroleum biodegradation products on phytoplankton growth. ArchiMer
Goutx, H; Berland, B; Leveau, M; Bertrand, J.
The effects of products resulting from petroleum biodegradation in continuous culture, have been studied on the growth of two marine algae. Sugars, lipids and amino acids are the main components resulting from petroleum degradation in the bacteria used medium. Their concentrations are respectively 600 mg.l super(-1), 46 mg.l super(-1) and 74.2 mu M.l super(-1). The algae growth is inhibited when products are enough concentrated; it is stimulated by low concentration. Among these products, lipids are more toxic than others. The two species show different sensitivity towards the used medium. Prorocentrum micans growth is more inhibited than that of Phaeodactylum tricornutum .
Tipo: Text Palavras-chave: Bacteria; Phytoplankton; Toxicity; Oil pollution; Crude oil; Biodegradation.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1017.pdf
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Interspecific differences in the effect of fish on marine microbial plankton ArchiMer
Villeger, Sebastien; Fouilland, Eric; Argenty, Jeremy; Bouvier, Corinne; Carre, Claire; Bouvier, Thierry.
The productivity of most marine ecosystems is limited by the availability of dissolved nitrogen (N) and phosphorus (P). Nutrient recycling is therefore a key process for ecosystem functioning. Fish recycle nutrients through the excretion of ammonia and phosphate and can influence the abundance and community structure of primary producers such as phytoplankton. However, the effect of fish on other plankton compartments, and whether all fish species have similar effects, is largely unknown. We used a tank experiment to test how 2 Mediterranean fish species, gilthead seabream Sparus aurata and golden mullet Chelon auratus, with distinctly different N and P excretion rates, can affect the abundance and community structure of 3 plankton compartments:...
Tipo: Text Palavras-chave: Marine fish; Nutrient excretion; Protozooplankton; Phytoplankton; Bacteria.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00584/69659/67585.pdf
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Effect of xanthan on the growth of sulphate-reduction bacteria in marine sediments. ArchiMer
Battersby, N; Stewart, D; Sharma, A.
Chemical additives are used extensively in the offshore oil industry. For secondary oil recovery, the biopolymer xanthan has been recommended as a viscosifying agent but its use may provoke microbial problems. The effect of adding xanthan to marine sediments on the growth and metabolic activity of sulphate-reducing bacteria (SRB) was studied. In all instances, its addition resulted in an increasing in SRB numbers of up to 250-fold. This was accompanied by a marked increase in the electron transport activity of the sediments when these were incubated under either aerobic or anaerobic conditions. The products formed as a result of the biodegradation of the polymer by indigenous heterotrophic bacteria in these sediments appear to have acted as suitable...
Tipo: Text Palavras-chave: Bacteria; Growth; Additives; Pollution effects; Oil recovery; Biodegradation; Polymers; Sediment pollution; Sulfate reduction.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1015.pdf
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Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus ArchiMer
Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R. S.; Dehairs, F.; Colaco, A..
High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using (HCO3-)-C-13 in the presence of sulphide, or...
Tipo: Text Palavras-chave: Mid-atlantic ridge; Hydrocarbon-seep mussel; Deep-sea mussels; Hydrothermal-vent; Puteoserpentis bivalvia; Mytilidae; Methane; Endosymbionts; Bacteria; Metabolism.
Ano: 2008 URL: https://archimer.ifremer.fr/doc/00207/31865/30271.pdf
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Factors affecting bacterial biomass and growth in the Duplin River estuary and coastal Atlantic Ocean. ArchiMer
Fallon, R; Newell, S; Sherr, B; Sheer, E.
Bacterial numbers and growth rates were measured in coastal, planktonic systems of the Western Atlantic ocean. Seasonal responses in growth rates of the bacterial assemblage were present at some sites, but these may be related to seasonal changes in substrate supply rather than to changes in temperature. Bacterial growth rates tended to decline with increasing distance from shore. There was evidence for predator-prey population cycles in the bacteria and < 17 mu m protozoa in the Duplin River Estuary which may indicate that the bacterial assemblage serves as prey for protozoan populations. In summer, a significant negative correlation between mu (instantaneous growth rate) and cell numbers in the bacterial assemblage indicated that substrate limitation...
Tipo: Text Palavras-chave: Protozoa; Plankton; Bacteria; Predators; Grazing; Temperature effects; Seasonal variations; Limiting factors; Interspecific relationships; Nutrients (mineral); Growth.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-955.pdf
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Fecal contamination in coastal areas: An engineering approach ArchiMer
Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf
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Unlike the Escherichia coli counterpart, archaeal RNase HII cannot process ribose monophosphate abasic sites and oxidized ribonucleotides embedded in DNA ArchiMer
Malfatti, Matilde Clarissa; Henneke, Ghislaine; Balachander, Sathya; Koh, Kyung Duk; Newnam, Gary; Uehara, Ryo; Crouch, Robert J.; Storici, Francesca; Tell, Gianluca.
The presence of ribonucleoside monophosphates (rNMPs) in nuclear DNA decreases genome stability. To ensure survival despite rNMP insertions, cells have evolved a complex network of DNA repair mechanisms, in which the ribonucleotide excision repair pathway, initiated by type 2 ribonuclease H (RNase HII/2), plays a major role. We recently demonstrated that eukaryotic RNase H2 cannot repair damaged, that is, ribose monophosphate abasic (both apurinic or apyrimidinic) site (rAP) or oxidized rNMP embedded in DNA. Currently, it remains unclear why RNase H2 is unable to repair these modified nucleic acids having either only a sugar moiety or an oxidized base. Here, we compared the endoribonuclease specificity of the RNase HII enzymes from the archaeon Pyrococcus...
Tipo: Text Palavras-chave: Ribonuclease; Bacteria; Escherichia coli (E coli); Archaea; Oxidative stress; Abasic-ribose; Oxidized-ribonucleotides; Pyrococcus abyssi; Type 2 RNase H.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00506/61796/65801.pdf
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Diversité phylogénétique et fonctionnelle des communautés microbiennes incultivées des sédiments marins de la marge de Sonora, Bassin de Guaymas (Golfe de Californie) ArchiMer
Vigneron, Adrien.
At continental margins, and more particularly in cold seep areas, microbial and animal communities were locally detected at the surface of the sediments. These communities grow using reduced chemical compounds (H2S, Methane, COZ ...) contained in the percolated cold fluids and produced by both geological and microbial processes. ln order to study microbial community diversity in these ecosystems and their role in the environment as well as to understand the environmental factors influencing the distribution and ecophysiology of these communities, surface (0-20 cmbsf) but also deeper (<9 mbsf) sediments were collected at the Sonora Margin. Microbial communities have been studied using various molecular, cultural and microscopy approaches. This research...
Tipo: Text Palavras-chave: Fluides froids; Marge de Sonora; Bassin de Guaymas; Diversité moléculaire; Communautés microbiennes; Archaea; Bacteria; Méthanogènes; Methanogens; Microbial communities; Molecular diversity; Guaymas Basin; Sonora Margin; ANME; SRb; Methane; Sediments; Cold seeps.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00498/60956/64359.pdf
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An abyssal mobilome: Viruses, plasmids and vesicles from deep-sea hydrothermal vents ArchiMer
Lossouarn, Julien; Dupont, Samuel; Gorlas, Aurore; Mercier, Coraline; Bienvenu, Nadege; Marguet, Evelyne; Forterre, Patrick; Geslin, Claire.
Mobile genetic elements (MGEs) such as viruses, plasmids, vesicles, gene transfer agents (GTAs), transposons and transpovirions, which collectively represent the mobilome, interact with cellular organisms from all three domains of life, including those thriving in the most extreme environments. While efforts have been made to better understand deep-sea vent microbial ecology, our knowledge of the mobilome associated with prokaryotes inhabiting deep-sea hydrothermal vents remains limited. Here we focus on the abyssal mobilome by reviewing accumulating data on viruses, plasmids and vesicles associated with thermophilic and hyperthermophilic Bacteria and Archaea present in deep-sea hydrothermal vents.
Tipo: Text Palavras-chave: Deep-sea hydrothermal vent; Bacteria; Archaea; (Hyper-)thermophiles; Mobilome.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37145/36731.pdf
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Stress factors resulting from the Arctic vernal sea-ice melt : Impact on the viability of bacterial communities associated with sympagic algae ArchiMer
Amiraux, Rémi; Burot, Christopher; Bonin, Patricia; Massé, Guillaume; Guasco, Sophie; Babin, Marcel; Vaultier, Frédéric; Rontani, Jean-françois.
During sea-ice melt in the Arctic, primary production by sympagic (sea-ice) algae can be exported efficiently to the seabed if sinking rates are rapid and activities of associated heterotrophic bacteria are limited. Salinity stress due to melting ice has been suggested to account for such low bacterial activity. We further tested this hypothesis by analyzing samples of sea ice and sinking particles collected from May 18 to June 29, 2016, in western Baffin Bay as part of the Green Edge project. We applied a method not previously used in polar regions—quantitative PCR coupled to the propidium monoazide DNA-binding method—to evaluate the viability of bacteria associated with sympagic and sinking algae. We also measured cis-trans isomerase activity, known to...
Tipo: Text Palavras-chave: Bacteria; Sea ice; Sympagic algae; Ice biota; Viability; Stress factors; Salinity; Bactericidal free fatty acids; Carbon export.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00681/79329/81840.pdf
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Adaptive features of a bacteriolytic enzyme from the style of the mussel Choromytilus meridionalis in response to environmental fluctuations. ArchiMer
Seiderer, L; Robb, F.
The extracellular digestive apparatus of bivalve molluscs consists of a crystalline style containing diverse degradative enzymes which are released during rotation and dissolution of the style. This paper reviews the occurrence of a temperature-regulated bacteriolytic enzyme in the black mussel Choromytilus meridionalis , which is an inhabitant of coastal reefs on the south and south west coast of South Africa. This agent is unable to lyse Micrococcus luteus or Escherichia coli , both of which are susceptible to lysis by a true lysozyme. It appears to have a restricted spectrum of activity which includes both free-living and kelp-associated gram-negative bacteria. In addition, the level of activity varies dramatically according to the environmental water...
Tipo: Text Palavras-chave: Choromytilus meridionalis; Bivalvia; Digestive system; Bacteria; Upwelling; Environmental effects; Adaptations; Bacteriocides; Marine molluscs; Enzymatic activity.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-991.pdf
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Nitrate respiration and nitrification in estuarine sediments. ArchiMer
Herbert, R.
Seasonal data and depth profiles using super(15)N-labelled NO sub(3)u- show that denitrification (77-90% of NO sub(3)u- respired) rather than NO sub(3)u- dissimilation to NH sub(4)u+ was the principal route of nitrate reduction in Kingoodie Bay sediments. Populations of both groups of NO sub(3)u- reducing bacteria were highest in the 0-20 mm horizon in those sediments where highest rates of NO sub(3)u- respiration were recorded (28.56 mu g N.d super(-1) dry wt. sediment super(-1)). Autotrophic nitrification rates shared a marked seasonality with highest rates (0.92 mu g N.d super(-1).g dry wt. sediment super(-1)) occurring during the summer. Maximum populations of autotrophic nitrifying bacteria were also found in the 0-20 mm sediment horizon and these...
Tipo: Text Palavras-chave: Seasonal variations; Bacteria; Estuaries; Sediment chemistry; Biogeochemistry; Ammonia; Nitrates; Nitrogen cycle.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-971.pdf
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Benthic foraminifera from the deep-water Niger delta (gulf of Guinea): Assessing present-day and past activity of hydrate pockmarks ArchiMer
Fontanier, Christophe; Koho, K. A.; Goni-urriza, M. S.; Deflandre, Bruno; Galaup, S.; Ivanovsky, A.; Gayet, Nicolas; Dennielou, Bernard; Gremare, Antoine; Bichon, S.; Gassie, C.; Anschutz, P.; Duran, R.; Reichart, G. J..
We present ecological and isotopic (δ18O and δ13C) data on benthic foraminifera sampled from 4 deep-sea stations in a pockmark field from the deep-water Niger delta (Gulf of Guinea, Equatorial Atlantic Ocean). In addition, a series of sedimentological and (bio)geochemical data are shown to back up foraminiferal observations. All stations are located within 1.2 km of each other, so prevailing oceanographic conditions can be assumed to be similar at each site. Two of the sites (GMMC-01 and GMMC-02) are located in a pockmark (named “pockmark A”) where current methane seepages were recorded by ROV observations. A third station (GMMC-03) is located in the topographic depression interpreted as a collapsed pockmark (named “pockmark B”). The fourth site (GMMC-04)...
Tipo: Text Palavras-chave: Niger delta; Hydrate pockmark; Foraminifera; Stable isotopes; Bacteria.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00211/32194/30628.pdf
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Nutrition experimentale: Problemes methodologiques lies a l'utilisation des microorganismes comme source trophique ArchiMer
Guidi, L.
The present study deals with technical problems encountered under lab conditions in studies of trophic relations between deposit-feeding invertebrates and microorganisms. Using 2 pure cultures of Pseudomonas and Navicula , it was shown that these microorganisms will attach to artificial (glass beads) and natural (sand grains) substrates. Best microbial attachment was observed on sand (up to 4.8 x 10 super(8) cells.g super(-1) dry wt.). The radioactive labelling of the microorganisms depends not only on the form under which the isotope ( super(14)C) is supplied to the cultures, but also on the time and the duration of the labelling. 70% of the isotope was incorporated in a stable manner by the diatoms. The bacteria incorporated, at most, 34% of the...
Tipo: Text Palavras-chave: Navicula; Pseudomonas; Bacillariophyceae; Algae; Bacteria; Biological attachment; Radioactive tracers; Methodology; Microorganisms; Trophic relationships; Microbiological culture; Zoobenthos; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-987.pdf
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