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Fed batch enzymatic saccharification of food waste improves the sugar concentration in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae H058 BABT
Yan,Shoubao; Yao,Jianming; Yao,Liming; Zhi,Zhijun; Chen,Xiangsong; Wu,Jingyong.
The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food waste; Enzymatic hydrolysis; Hydrolysates; Fermentation; Ethanol production.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200002
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Identification of yeasts Isolated from processed and frozen cocoa (Theobroma cacao) pulp for wine production BABT
Trindade,Rita de Cássia; Resende,Maria Aparecida de; Barreto,Eriana Gomes Serpa; Mendes,Taniella de Carvalho; Rosa,Carlos Augusto.
The alternative use of cocoa (Theobroma cacao) for wine production was tested. The pulp samples, obtained from Formosa farm, Itacaré, Brazil, were diluted, homogenized and inoculated on Sabouraud dextrose agar medium (SDA) and incubated at 28º C for 5-8 days. Selected colonies were tested for the ability to ferment cocoa pulp and divided into fermentative, non-fermentative and weak/late fermentative species. Isolates characterized as fermentative were further tested in a small-scale wine production plant and identified. Species from the genus Brettanomyces constituted the main fermentative yeasts, with the exception of two Kloeckera apis samples. The final wine product was normally pale or clear, making clarification unnecessary, and with a sweet or dry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Cocoa pulp; Fermentation; Wine production; Brettanomyces sp.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300013
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Extraction of Indigo from Some Isatis species and Dyeing Standardization Using Low-technology Methods BABT
Comlekcioglu,Nazan; Efe,Lale; Karaman,Sengul.
Fresh leaves of four Isatis species culture form of I. tinctoria L and wild forms of I. buschiana Schischkin, I. candolleana Boiss. (endemic) and I. tinctoria L. subsp. corymbosa. (Boiss.) were used for indigo production. Dyes were extracted by fermentation and hot water application. The extracted dyes were optimized with different pH and reducing agents. Results showed that the dye from hot water application produced the desired dying quality at pH 11. Reducing agent concentrations had no significant effect on color quality. Dark blue and blue colors were obtained from I. tinctoria and I. candolleana extracts although I. tinctoria subsp. corymbosa and I. buschiana produced mostly yellow-gray colors. Light, dry and wet rubbing fastness values varied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isatisspp.; Woad; Indigo; Natural dye; Fermentation; Fastness; Spectrophotometry.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100096
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Produção de Lactobacillus plantarum em melaço de cana-de-açúcar BABT
Feltrin,Valdemar P.; Sant'Anna,Ernani S.; Porto,Anna C. S.; Torres,Regina C. O..
Comparative studies of Lactobacillus plantarum were carried out in MRS broth (control) and in 3% (w/v) sugar cane blackstrap molasses enriched as follows: 0.5% yeast extract, 0.5% ammonium citrate and 0.5% sodium acetate (Medium 1) and 0.5% yeast extract, 0.5% ammonium citrate, 0.5% sodium acetate, 0.5% ammonium phosphate, 0.2% ammonium citrate, 0.1% Tween 80 and 0.005% manganese sulphate (Medium 2). The experiment, was carried out at a 5 L fermentor, with 3.5 L as working volume. At the end of the fermentation period MRS presented the highest viable cells production (28.68 log CFU/mL) while in Medium 2 it was 25.80 and in Medium 1 19.36 log CFU/mL. Biomass productivity was 0.0865 g.L-1.h for MRS (control), 0.0768 and 0.0507 g.L-1.h for Medium 2 and Medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Fermentation; Molasses.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100015
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Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology BABT
Stergiou,Panagiota-Yiolanda; Foukis,Athanasios; Theodorou,Leonidas; Papagianni,Maria; Papamichael,Emmanuel.
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular enzymes; Α-amylase; Kluyveromyces marxianus; Fermentation; RSM.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016
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A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol BABT
Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte.
This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017
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Evaluation of emulsifier stability of biosurfactant produced by Saccharomyces lipolytica CCT-0913 BABT
Lima,Álvaro Silva; Alegre,Ranulfo Monte.
Surface-active compounds of biological origin are widely used for many industries (cosmetic, food, petrochemical). The Saccharomyces lipolytica CCT-0913 was able to grow and produce a biosurfactant on 5% (v/v) diesel-oil at pH 5.0 and 32ºC. The cell-free broth emulsified and stabilized the oil-in-water emulsion through a first order kinetics. The results showed that the initial pH value and temperature influenced the emulsifier stability (ES), which was the time when oil was separated. The biosurfactant presented different stabilization properties for vegetable and mineral oil in water solution, despite the highest values of the ES occurring with vegetable oil. The biosurfactant presented smallest ES when compared to commercial surfactants; however, this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Emulsifier stability; Fermentation; Vegetable oil; Diesel oil.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200004
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Alcoholic fermentation by the wild yeasts under thermal, osmotic and ethanol stress BABT
Silva,Rosimeire Oenning da; Batistote,Margareth; Cereda,Marney Pascoli.
This study aimed to explore the variability in the metabolism of nine wild yeasts isolated from the sugarcane juice from a distillery in the Brazilian State of Mato Grosso. Cell viability under the stress conditions was evaluated. The yeasts were inoculated in the test tubes containing sugarcane juice adjusted from 12 to 21º Brix, ethanol from 6 to 12% in volume and temperature at 30, 35 and 40ºC. The viability was established by the growth in Petri dishes and visually by the CO2 production in the test tubes. None of the evaluated yeasts showed simultaneous resistance to the three stress conditions. The potential of yeast BB.09 could be emphasized due to its ability to ferment up to12% ethanol at 30°C.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio prospection; Alcohol distillery; Cellular viability; Fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200001
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Solid-state fermentation for the enrichment and extraction of proteins and antioxidant compounds in rice bran by Rhizopus oryzae BABT
Kupski,Larine; Cipolatti,Eliane; Rocha,Meritaine da; Oliveira,Melissa dos Santos; Souza-Soares,Leonor de Almeida; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the solid-state fermentation with Rhizopus oryzae CCT 7560 of rice bran for the enrichment of proteins and the antioxidant compounds in the fermented biomass. Fermentation was performed in tray bioreactors at 30ºC for 120 h. Protein extraction was done at alkaline pH, followed by precipitation with acetone. Phenolic compounds were extracted with cold methanol. The maximum protein was recovered from after 120 h (26.6%). The content of total phenolic compounds increased during the fermentation and was maximum after 96 h, which inhibited the DPPH radical by 87%. The promising characteristics of the protein and phenolic extracts of the biomass suggested the application in the coating composition for vegetal tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein extract; Phenolic extract; Fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600018
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Effect of deoxynivalenol and T-2 toxin in malt amylase activity BABT
Garda-Buffon,Jaqueline; Baraj,Edlira; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aminolitic enzymes; Fermentation; Trichothecenes; Barley.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300002
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Comparison between two selected Saccharomyces cerevisiae strains as fermentation starters in the production of traditional cachaça BABT
Gomes,Fátima de Cássia Oliveira; Araújo,Roberta Amália de Carvalho; Cisalpino,Patrícia Silva; Moreira,Elizabeth Spangler Andrade; Zani,Carlos Leomar; Rosa,Carlos Augusto.
Two Saccharomyces cerevisiae strains were tested as the starter yeasts in a traditional cachaça distillery. The strains used were S. cerevisiae UFMG-A829, isolated from a cachaça fermentation process, and S. cerevisiae K1-V1116, obtained from the wine industry. The permanence of each strain in the fermentation must was determined by RAPD (Random Amplified Polymorphic DNA)-PCR, with primer M13. Both yeast strains were prevalent in the vats for approximately 30 days. Indigenous non-Saccharomyces and indigenous S. cerevisiae strains were isolated in lower counts during the fermentation period. Indigenous S. cerevisiae strains were molecularly distinct when compared to the starter yeasts. The two yeasts appeared promising starter yeasts in the fermentation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cachaça; Saccharomyces cerevisiae; Starter strains; Fermentation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200023
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Production of Propionic Acid by Propionibacterium acidipropionici from Agroindustrial Effluents BABT
Teles,Jéssica Cristine; Stolle,Elaine Macedo; Koloda,Stela Aparecida; Barana,Ana Claudia.
Abstract The purpose of this paper was to evaluate the production of propionic acid from the fermentation of agroindustrial effluents using a Propionibacterium acidipropionici culture. The composition of the substrates was determined by using an experimental design of mixtures, resulting in 10 trials. The substrates were fermented in batch borosilicate glass reactors at a temperature of 35°C, initial pH of 6.5, and 20 mL.L-1 of inoculum suspension. The highest yield of propionic acid production, 0.79 g of product per g of substrate, was obtained with a substrate composed only of corn steep liquor, which showed a productivity of 5.20 mg.L-1h-1 and production of 0.40mL.L-1. These results showed that the corn steep liquor positively influenced performance and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroindustrial by-products; Fermentation; Corn steep liquor; Cheese whey; Wastewater.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100702
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Enhanced Production Of Streptokinase By Chemical Mutagenesis Of Streptococcus agalactiae EBL-20 BABT
Arshad,Arooj; Zia,Muhammad Anjum; Asghar,Muhammad; Joyia,Faiz Ahmad.
Abstract Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases. The current study focused on the enhanced production of SK by inducing mutation in Streptococcus agalactiae EBL-20 and optimization of medium components and culture conditions for the maximum growth of mutant derived strain. S. agalactiae EBL-32 was selected as a potent mutant after exposure of S. agalactiae EBL-20 to EMS for 180 minutes. SK activity obtained from mutant derived strain was found to be 1.6 fold higher as compared to the activity achieved by wild strain. Nutritional requirements of the mutated strain were optimized by single factor analysis method suggesting glucose as the optimum carbon source; yeast extract and peptone as a suitable nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethyl methanesulfonate; Fermentation; Response surface methodology; Streptokinase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100200
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The use of the oregano (Origanum vulgare L.) essential oil and hydrosol in green olive fermentation BABT
Özcan,M. Musa; Arslan,Derya; Aydar,Ali Osman.
The effect of oregano the (Origanum vulgare L.) oil at the levels of 0.05, 0.1 and 0.3% and oregano hydrosol on the physicochemical, microbiological and sensory characteristics of the green olive (Edremit variety) fermentation was investigated. The initial pH of the oregano oil added samples were higher than the other treatments, which were above 5.8. The highest final acidity was observed in the hydrosol+brine combination (0.53%). The initial LAB population on the first day of the fermentation was high in the diluted hydrosol (8.89 log cfu ml-1) and control (8.47 log cfu ml-1) samples. But a significant difference was not observed between the LAB counts of the treatments on the 40th day of fermentation. The control and brine+oregano hydrosol samples had...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green olive; Fermentation; Origanum vulgare L.; Essential oil; Hydrosol.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300022
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Sugar cane juice fermentation by Zymomonas mobilis CP4 subjected to inhibition by ethanol and high initial concentration of substrate BABT
Tano,Marcia S.; Buzato,João B.; Celligoi,Maria Antonia P. C..
Sugar cane juice fermentation by Z. mobilis CP4 subject to stress by ethanol and high concentration of sucrose was investigated. Supplementation with saponifiable portion of soybean oil deodourising destillate (SOD) at 5; 10 and 15 mL/L.The ethanol production resulted values of 15.91; 16.99 and 15.63 g/L respectively. Values of Yp/s 0.35; 0.36 and 0.37 were achieved, which represented 20.69; 24.14 and 27.59% respectively higher when unsupplemented medium fermentation was carried out.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Fermentation; Lipids; Ethanol.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400012
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Effect of biomass reduction on the fermentation of cider BABT
Nogueira,Alessandro; Mongruel,Caroline; Simões,Deise Rosana Silva; Waszczynskyj,Nina; Wosiacki,Gilvan.
The aim of this work was to determine the influence of biomass reduction in the cider processing and the quality of the fermented product made on laboratory scale, but in the same conditions usually found in factory units. The must, made with apples of the Gala variety, depectinized and transferred to 500 mL-fermenters, was inoculated with 2.0x10(6) cfu/mL of natural or commercial yeast, and at each 12 hours biomass was removed by centrifugation in one flask of the experimental set, and the must was left to ferment. All seven flasks of the fermented beverage were analyzed for 20-26 days after the inoculation, and the results showed that the best moment for biomass removal was 1.5-2.0 days of fermentation, leading to a product with significant residual...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cider; Fermentation; Technology.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700019
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Date and their processing byproducts as substrates for bioactive compounds production BABT
Tang,Zhen-Xing; Shi,Lu-E; Aleid,Salah Mohammed.
Date is the most popular fruit in middle-east countries. However, the date production process is accompanied by a substantial increase of loss during picking, storage, commercialization and conditioning process. Date and their byproducts have many essential elements for the growth of microorganisms. Thus, they can be converted into value-added compounds through biotechnology. In this paper, date and their processing byproducts used as substrates for producing value-added products such as organic acids, exopolysaccharide, antibiotics, date flavored probiotic fermented dairy, bakery yeast, etc, were reviewed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Date; Byproduct; Biotechnology; Fermentation; Review.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500706
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"A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain" BJM
Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.
The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae, 1.7% for S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii; Saccharomyces rouxii.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025
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Isolation of fungi from dung of wild herbivores for application in bioethanol production BJM
Makhuvele,Rhulani; Ncube,Ignatious; Jansen van Rensburg,Elbert Lukas; La Grange,Daniël Coenrad.
ABSTRACT Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides. Due to the presence of residual partially degraded lignocellulose in the gut, the dung of herbivores can be considered as a natural source of pre-treated lignocellulose. A total of 101 fungi were isolated (36 yeast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignocellulase; Biofuel; Fungi; Xylose; Fermentation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400648
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Selection of starter cultures for the production of sour cassava starch in a pilot-scale fermentation process BJM
Penido,Fernanda Corrêa Leal; Piló,Fernanda Barbosa; Sandes,Sávio Henrique de Cicco; Nunes,Álvaro Cantini; Colen,Gecernir; Oliveira,Evelyn de Souza; Rosa,Carlos Augusto; Lacerda,Inayara Cristina Alves.
ABSTRACT Sour cassava starch (Polvilho azedo) is obtained from a spontaneous fermentation conducted by microorganisms from raw materials and fermentation tanks. This product is traditionally used in the baking industry for the manufacture of biscuits and Brazilian cheese breads. However, the end of fermentation is evaluated empirically, and the process occurs without standardization, which results in products of inconsistent quality. Predominant microbiota from a cassava flour manufacturer was isolated in order to select starter cultures for the production of sour cassava starch in a pilot-scale fermentation process. Lactic acid bacteria and yeasts were isolated, enumerated and grouped by Restriction Fragment Length Polymorphism, and PCR fingerprinting,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Yeasts; Starter cultures; Fermentation; Bakery products.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400823
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