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| Registros recuperados: 109 | |
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| Lu,J.; Ding,Y.; Yi,X.; Zheng,J.. |
| T lymphocytes are important in the pathogenesis of psoriasis, and increasing evidence indicates that B cells also play an important role. The mechanisms of action, however, remain unclear. We evaluated the ratios of CD19+ B cells in peripheral blood mononuclear cells (PBMCs) from 157 patients with psoriasis (65 patients with psoriasis vulgaris, 32 patients with erythrodermic psoriasis, 30 patients with arthropathic psoriasis, and 30 patients with pustular psoriasis) and 35 healthy controls (HCs). Ratios of CD19+ B cells in skin lesions were compared with non-lesions in 7 erythrodermic psoriasis patients. The Psoriasis Area Severity Index (PASI) was used to measure disease severity. CD19+ B cell ratios in PBMCs from psoriasis vulgaris (at both the active... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psoriasis; B-lymphocytes; B-lymphocyte subsets; Psoriasis Area Severity Index (PASI); Flow cytometry. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900706 |
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| Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui. |
| Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009 |
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| Martins,Gabrielle R.; Marinho,Rebeca C.; Bezerra Junior,Rosivaldo Q.; Alves,Antoniel de O.; Câmara,Lilia M.C.; Albuquerque-Pinto,Luiz C.; Teixeira,Maria F. da S.. |
| Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CAEV; MVV; Cell culture; MSC; Flow cytometry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100125 |
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| Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama. |
| Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009 |
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| Vancin,Franciele Rampazzo; Webber,Bruna; Bondan,Carlos; Daroit,Luciane; Santos,Luciana Ruschel dos; Rodrigues,Laura Beatriz. |
| ABSTRACT: The preservation of milk samples for microbiological analyses by the Brazilian Network of Milk Quality Control Laboratories requires the addition of preservatives to maintain the microbiota from the time of sample collection to the moment of analysis. The number of microorganisms can change as a result of the active ingredients and concentration of the preservative, as well as due to interactions between the preservatives, incubation time, and packaging temperature. The objective of this research was to evaluate the conservation potential of different concentrations of sodium azide and chloramphenicol on the analytical shelf life of milk samples. Two farms were selected, one with a low bacterial count and one with a high bacterial count. The milk... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Azidiol; Individual bacterial count; Flow cytometry. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200750 |
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| Registros recuperados: 109 | |
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