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Contribution of several nitrogen sources to growth of Alexandrium catenella during blooms in Thau lagoon, southern France ArchiMer
Collos, Y; Vaquer, A; Laabir, M; Abadie, Eric; Laugier, Thierry; Pastoureaud, Annie; Souchu, Philippe.
A monitoring program with a weekly sampling frequency over a 15-month period indicates that urea concentrations above a certain threshold level may trigger the blooms of Alexandrium catenella in Thau lagoon. However, urea concentrations were also sometimes related to ammonium and dissolved organic nitrogen concentrations, indicating that the role of urea may not be a direct one. An original approach is used to assess the relative contribution of several nitrogen sources (nitrate, nitrite, ammonium, urea) to growth of A. catenella by comparing nitrogen uptake rates to nitrogen-based growth rates estimated from dilution experiments during four blooms over a 4-year period (2001-2004) in Thau lagoon. Nitrate and nitrite contributed 0.1-14% and 0.1-5%...
Tipo: Text Palavras-chave: Uptake kinetics; Ammonium; Urea; Growth; Nitrogen; HAB; Alexandrium catenella.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-6454.pdf
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Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea ArchiMer
Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf
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The use of high rate algal ponds for the treatment of marine effluent from a recirculating fish rearing system ArchiMer
Pagand, Pascal; Blancheton, Jean-paul; Lemoalle, J; Casellas, C.
A high rate algal pond (HRAP) system was used to treat effluent from a recirculating sea water aquaculture system in southern France. Dicentrarchus labrax L. were farmed at a high density, with effluents containing an average of 10 mg L-1 dissolved inorganic nitrogen (DIN) and 1.3 mg L-1 reactive phosphorus (RP). On a yearly basis, the algal pond removed 59% of the dissolved nitrogen and 56% of the phosphorus input, which was converted into 3.3 kg DW m(-2) algae. Green macroalgae were dominant throughout the year and the algal biomass mirrored the seasonal changes in daily irradiance and temperature. This first year study supports the possibility of treating marine aquaculture wastes using HPAPs, although conditions will have to be found to mitigate the...
Tipo: Text Palavras-chave: Nitrogen; Biofilters; Irradiance; Temperature; Photosynthesis; Growth; Culture; Chlorophyta; Nutrient Removal.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-515.pdf
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Bilan azoté des flux tidaux d'un marais salé à végétation halophyte ArchiMer
Silva, Celine; Raillard, Marieke; Bodoy, Alain; Hussenot, Jerome.
In spring 2001, during a complete tidal cycle, nitrogen fluxes between the sea and the salt marsh were calculated. The marsh (1198 m2) covered in 75 % by halophytes, with 46 % by the sea purslane, Halimione portulacoides. The nitrogen balance shows that the salt marsh imports nitrogen micro-particules (2,09 g) by sedimentation, exports dissolved nitrogen (4,13 g), and that the macro-element part (-1,02 g in fauna and vegetable refuse) in the global mass balance (3,06 g) is not unimportant. Nitrogen fluxes are mainly under inorganic form for the incoming flux (mainly NO3), and under organic form for the outgoing flux. The halophyte vegetation of this marsh seems to play thus a dominating role in the tidal nitrogen exchanges, by a mechanical action on the...
Tipo: Text Palavras-chave: Marais salé; Halophytes; Azote; Bilan de masse; Halimione portulacoides; Salicornes; Salt marsh; Halophytes; Nitrogen; Mass balance; Halimione portulacoides; Sea purslane; Sampshire.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00045/15597/13000.pdf
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Evolution des caractéristiques chimiques et biologiques des sédiments en circuit clos. I. Azote et populations bactériennes en fonction de certains paramètres du milieu ArchiMer
Chamroux, S.; Boucher, G..
Chemical and Biological evolution of sediments in closed circuit : I. Nitrogen and bacteria. A series of well controlled sea water closed circuit sand filters al­lowed the study of chemical and biological regulation processes in the sediment. Bacteria, Nitrogen chemistry and meiofauna were mainly investigated. The evolution of the different forms of nitrogen is rather similar, whatever the organic matter flow in the sediment. As long as the amount of or­ganic input works as limiting factor, bacteria densities are proportional to its flow. No correlation was found between bacteria and chemical parameters in the sand or in the water. The temperature does not seem to play a determinant part neither in the evolution of bacteria nor in the mineralization of...
Tipo: Text Palavras-chave: Circuit clos; Sédiment; Bactéries; Matière organique; Azote; Closed circuit; Sediment; Bacteria; Organic matter; Nitrogen.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00305/41652/40861.pdf
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High-rate algal pond treatment for water reuse in an integrated marine fish recirculating system: effect on water quality and sea bass growth ArchiMer
Deviller, Genevieve; Aliaume, Catherine; Franco, Miguel; Casellas, Claude; Blancheton, Jean-paul.
A high-rate algae pond (HRAP) was tested as a second loop of water treatment in a recirculating fish rearing system to reduce water requirements and nutrient discharge levels. Three duplicated groups of sea bass (mean initial body weight 3 5 I I g) were reared under different system conditions (flow-through system, recirculating system and recirculating system with HRAP) for I year. Fish survival rate was higher in the system with HRAP, and their mean body weight was statistically higher (p<0.05) during the month of maximal climatic conditions for algae photosynthesis. After 266 days of experiment, mean fish weight was significantly higher (p<0.05) in the flow-through system than in the recirculating systems. Final fish weight was 15% higher in the...
Tipo: Text Palavras-chave: Sustainability; Phosphorus; Nitrogen; Fish growth; Recirculation; High rate algae pond.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-620.pdf
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Biogeochemical aspects of bottom anoxia in a Mediterranean lagoon (Thau, France) ArchiMer
Souchu, Philippe; Gasc, Anne; Collos, Y; Vaquer, A; Tournier, Henri; Bibent, B; Deslous-paoli, Jean-marc.
Physical and chemical characteristics (temperature, salinity, dissolved oxygen), nutrients [dissolved inorganic nitrogen (DIN), soluble reactive phosphorus (SRP) and silicate], dissolved organic nitrogen (DON) and phosphorus and particulate matter [particulate organic carbon (POC) and nitrogen (PON) and chlorophyll a] were measured at a station located in the deepest part of the Thau lagoon (8.5 m), France, during a 10 d period of bottom anoxia in summer 1994. The upper 8 cm of sediment were also analyzed for ammonium (NH(4)(+)) and SRP concentrations in the porewater. The study period was characterized by mean wind speed under 4 m s(-1) which induced an increase of surface temperature from 18 to 29 degrees C with the formation of a thermocline. The...
Tipo: Text Palavras-chave: Anoxia; Nitrogen; Phosphorus; Eutrophication; Thau lagoon; Mediterranean Sea.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00070/18142/15694.pdf
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Variabilité hydrologique et biologique du golfe du Lion. I. Transports en azote et productivité potentielle ArchiMer
Conan, P; Pujo-pay, M; Raimbault, P; Leveau, M.
A monthly time-series of the seasonal evolution of the North Mediterranean Current (NMC) and of nitrogen flux across a transect (5 degrees 12'5 E between 43 degrees 10'N and 42 degrees 50'N) at the entrance of the Gulf of Lions was carried out over two years. The NMC exhibited two distinct structures: a winter structure (from November to May) characterised by strong mesoscale and interannual variations in which the current was 20 to 30 km width, about 400 m depth and velocity scaled between 5 and more than 40 cm s(-1); and a summer structure (from May to November) which was less sensitive to mesoscale variations but showing large interannual variability. The current was located more offshore, was wider and shallower than the one in winter and velocity...
Tipo: Text Palavras-chave: Hydrologie; Variabilité à méso-échelle; Azote; Flux de matière; Bilan; Hydrology; Mesoscale variability; Nitrogen; Flux; Budget.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00325/43638/43249.pdf
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Trophic dynamics of scleractinian corals: stable isotope evidence ArchiMer
Tremblay, Pascale; Maguer, Jean-francois; Grover, Renaud; Ferrier-pages, Christine.
Reef-building corals form symbioses with dinoflagellates from the diverse genus Symbiodinium. This symbiotic association has developed adaptations to acquire and share nutrients, which are essential for its survival and growth in nutrient-poor tropical waters. The host is thus able to prey on a wide range of organic food sources (heterotrophic nutrition) whereas the symbionts acquire most of the inorganic nutrients (autotrophic nutrition). However, nutrient fluxes between the two partners remain unclear, especially concerning heterotrophically acquired carbon and nitrogen. We combined physiological measurements and pulse-chase isotopic labeling of heterotrophic carbon and nitrogen, as well as autotrophic carbon to track nutrient fluxes in two coral...
Tipo: Text Palavras-chave: Autotrophy; Carbon; Heterotrophy; Irradiance; Isotopic labeling; Nitrogen; Stylophora pistillata; Symbiodinium; Symbiosis; Turbinaria reniformis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00270/38145/73160.pdf
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Comparison of two methods for evaluating waste of a flow through trout farm ArchiMer
Roque D'Orbcastel, Emmanuelle; Blancheton, Jean-paul; Boujard, Thierry; Aubin, Joel; Moutounet, Yves; Przybyla, Cyrille; Belaud, Alain.
European water legislation enforces increasingly restrictive measures with regards to reduction of water consumption and waste emission in order to minimise the potential environmental impact of the agro industry sector. Fish farms are particularly concerned, but legislation covering effluent discharge varies significantly from country to country. However, recommendations and directives from institutional, national or regional bodies suggest the enforcement of increasingly strict waste reduction measures and the development of waste treatment. Before treatment, it is necessary to evaluate waste production in terms of composition and quantity. The waste quantification methods used today for fish culture systems are either based on direct measurements of...
Tipo: Text Palavras-chave: Phosphorus; Nitrogen; Suspended solids; Trout farm; Waste.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6822.pdf
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Spatio-temporal variation in diet may affect condition and abundance of juvenile European hake in the Gulf of Lions (NW Mediterranean) ArchiMer
Ferraton, Franck; Harmelin Vivien, Mireille; Mellon-duval, Capucine; Souplet, Arnauld.
Variations in space and time of juvenile hake diet (5 to 19 cm total length, TL) were investigated in the Gulf of Lions (NW Mediterranean) and related to variation in C and N stable isotope ratios, condition and abundance. Crustaceans (mysids and euphausiids) dominated the diet of the smallest juvenile hake (5 to 9 cm. TL), and fishes (sardines and anchovies) of the largest juveniles (15 to 19 cm TL). The transition from a crustacean- to a fish-based diet occurred in medium-sized juveniles (10 to 14 cm TL), which preyed on both crustaceans and fishes (gobiids). These juveniles preyed on fishes when living in shallow waters (30 to 50 m), and crustaceans when located in deep waters (70 to 150 m). Although hake diet did not change over time in shallow waters...
Tipo: Text Palavras-chave: Recruitment; Relative condition factor; Nitrogen; Carbon; Stable isotopes; Stomach contents; Merluccius merluccius.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2627.pdf
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Modélisation des cycles de l'azote et du phosphore dans les sédiments d'une lagune soumise à une exploitation conchylicole ArchiMer
Chapelle, Annie; Mesnage, Valérie; Mazouni, Nabila; Deslous-paoli, Jean-marc; Picot, Bernadette.
A simulation of nitrogen and phosphorus exchanges for the sediment-water interface is presented, The aim of the sttudy is to understand the dynamic behaviour of an eutrophie coastal lagoon (Thau, France), ln situ experiments were performed to estimate the different pools of nitrogen and phosphorus in the water column and in the sediment. The diffusive fluxes between them were also estimated. A numerical mode1, simulating nitrogen and phosphorus cycles, was developed and calibrated with data recorded .at two seasons (winter and summer). This study leads to the conclusion that is necessary to know the different pools of nitrogen and phosphorus in order to estimate which fraction is bioavailable and the rate of nutrient exchange between sediment and water. It...
Tipo: Text Palavras-chave: Modèle; Phosphore; Azote; Sédiment; Ecosystème conchylicole; Modelling; Phosphorus; Nitrogen; Sediment; Shellfish ecosystem.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00039/15026/12360.pdf
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Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia : phosphate an important factor ArchiMer
Lema, Anais Kimberley; Latimier, Marie; Nezan, Elisabeth; Fauchot, Juliette; Le Gac, Mickael.
The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with...
Tipo: Text Palavras-chave: Pseudo-nitzschia strains; Domoic acid; Ratio; Phosphate; Nitrogen.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00377/48849/49397.pdf
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Particulate Matter in Mangrove Forests and Seagrass Beds as a Nitrogen Source in Tropical Coastal Ecosystems ArchiMer
Gillis, Lucy G.; Bouma, Tjeerd J.; Cathalot, Cecile; Ziegler, Alan D.; Herman, Peter M..
We show in laboratory and field investigations that in the short-term seagrasses obtain most of their required nitrogen from the degradation of seagrass leaves, rather than degradation of leaves exported from adjacent mangroves. Mangrove forests at our Thailand site retain the majority of their nutrients, and therefore potentially buffer seagrasses from nutrients.
Tipo: Text Palavras-chave: Buffering; Mangrove forests; Nitrogen; Outwelling; Particulate organic material; Seagrass beds.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00266/37731/42021.pdf
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Isotopic composition and speciation of sedimentary nitrogen and carbon in the Okinawa Trough over the past 30 ka ArchiMer
Zheng, Li-wei; Hsiao, Silver Sung-yun; Ding, Xiao-dong; Li, Dawei; Chang, Yuan-pin; Kao, Shuh-ji.
Total organic carbon to total nitrogen ratios (C/N) and their isotopic compositions (δ13CTOC and δ15NTN) are oft-applied proxies to discern terrigenous from marine-sourced organics and to unravel ancient environmental information. In high depositional Asian marginal seas, the inclusion of N-bearing minerals in the bulk sediment dilutes or masks signals sought after in δ15N and δ13C analyses, thus leading to incorrect and even contradictory interpretations. We used KOH-KOBr to separate operationally defined total organic matter into oxidizable (labile) and residual fractions for content and isotope measurements. In a sediment core in the Okinawa Trough, significant amounts of carbon and nitrogen existed in the residual phase, in which the C/N ratio was ~9,...
Tipo: Text Palavras-chave: Carbon; Nitrogen; Isotope; Okinawa Trough; Sediment; Total organic carbon.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00295/40584/39503.pdf
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Betaine lipid and neutral lipid production under nitrogen or phosphorus limitation in the marine microalga Tisochrysis lutea (Haptophyta) ArchiMer
Huang, Bing; Marchand, Justine; Thiriet-rupert, Stanislas; Carrier, Gregory; Saint-jean, Bruno; Lukomska, Ewa; Moreau, Brigitte; Morant-manceau, Annick; Bougaran, Gael; Mimouni, Virginie.
Nitrogen (N) and phosphorus (P) limitations induce neutral lipid accumulation and membrane lipid remodeling in the domesticated oleaginous microalga Tisochrysis lutea. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of T. lutea throughout batch cultures under N or P limitation compared with non-limiting nutrient condition (NP). The results show that, under N limitation, the breakdown and re-use of cellular N-containing compounds contributed to carbohydrates and further triacylglycerol (TAG) accumulation, where the accumulation of docosahexaenoic acid (DHA) in neutral lipids might mainly be derived from the recycling of membrane polar lipids. Conversely, P limitation did not hinder the photosynthetic capacity;...
Tipo: Text Palavras-chave: Tisochrysis lutea; Phosphorus; Nitrogen; Lipid remodeling; Betaine lipid; Polyunsaturated fatty acid.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00492/60409/64776.pdf
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Role of tidal pumping on nutrient cycling in a temperate lagoon (Arcachon Bay, France) ArchiMer
Deborde, J; Anschutz, P; Auby, Isabelle; Gle, C; Commarieu, M; Maurer, Daniele; Lecroart, P; Abril, G.
The hypothesis of nutrient-rich pore-waters seeping at low tide through sediments to channel waters, which drain tidal flats during ebb, was evaluated in the Arcachon lagoon. The back of the bay is affected by freshwater inputs and underground freshwater discharges. The upper part of tidal flat consists of permeable sandy sediments, which are covered by a muddy sediment layer on the lower part. Permeable sediments outcrop in the bed of channel web. Surface water chemistry and early diagenesis processes in sediment were estimated by collecting channel web waters and cores on a tidal flat and in channels at different seasons and time scales. Waters from tidal creeks are under-oxygenated, and enriched in reduced solutes. Muddy sediments showed evidences of...
Tipo: Text Palavras-chave: Arcachon Bay; Pore waters seeping; Nitrogen; Phosphorus; Organic matter mineralization; Tidal creek water; Tidal pump.
Ano: 2008 URL: https://archimer.ifremer.fr/doc/2008/publication-3927.pdf
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Marée verte en martinique ArchiMer
Piriou, Jean-yves.
Few years ago, proliferations of the green seaweed Enteromorpha have been noticed on sorne beaches of the southem coast of Martinique (American French Island). A study, in the year 1992, shows that Enteromorpha has a seasonal development, that nitrogen is the limiting factor, and that its principal feeding cornes from urban discharges thrown away directly into growing areas.
Tipo: Text Palavras-chave: Azote; Enteromorpha; Martinique; Proliférations; Rejets urbains; Nitrogen; Enteromorpha; Martinique; Proliferations; Urban discharges.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00132/24277/22275.pdf
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Trophic resource partitioning of two snook fish species (Centropomidae) in tropical estuaries in Brazil as evidenced by stable isotope analysis ArchiMer
Guazzelli Gonzalez, Julio; Ménard, Frédéric; Le Loc'H, François; Agrelli De Andrade, Humber; Pontes Viana, Andréa; Ferreira, Valdimere; Frédou, Flávia Lucena; Lira, Alex Souza; Munaron, Jean-marie; Frédou, Thierry.
We investigated the trophic niches and the resource partitioning of two snook species, the common (Centropomus undecimalis) and the fat snook (C. parallelus), in four tropical estuaries of the northeastern Brazil, using stable isotope analyses of carbon (δ13C) and nitrogen (δ15N). Fish specimens, mainly juveniles, basal resources and a primary consumer were collected in 2015 during dry and rainy seasons in all estuaries, which exhibited differences in system size, geomorphologic shapes, levels of sea access and anthropic pressures. Potential effect of factors like fish body length, estuary and seasonality on isotope ratios were investigated. Positive relationships between the size of fish and δ15N values were found, regardless the species. Our results...
Tipo: Text Palavras-chave: Brackish water fish; Trophic relationships; Carbon; Nitrogen; Centropomus.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00506/61767/65745.pdf
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Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno.
Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied...
Tipo: Text Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf
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