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Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014. ArchiMer
Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells>20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (<3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf
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Evolution divergente de cultures phytoplanctoniques marines en relation avec la qualité des enrichissements ArchiMer
Divanach, P.; Sube, J..
Four marine phytoplanctonic cultures from natural inoculum of Nannodito sp. have been submitted to different fertilizers in identical light and tem­perature conditions. The composition of enrichments has been studied to introduce Nitrogen as comparative factor. One tank without nutrients and three enriched cultures, the first only with sodium nitrate, the second with "Conway medium" and the last in which nutri­ents were provided by convenient dilution of "foam", have been followed for phy­sical, chemical, biological parameters during three months. Temperature, pH, dis­solved oxygen, optical density, particulate matters, Organic Carbon, Nitrogen, Phos phorus, Calcium, Magnesium, Sodium, Potassium, metal traces and biological parame­ters were analysed...
Tipo: Text Palavras-chave: Enrichissements; Recyclage; Écume; Phytoplancton; Déchets; Aquaculture; Enrichments; Recycling; Foam; Phytoplankton; Wastes; Aquaculture.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00305/41662/42780.pdf
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The Underwater Vision Profiler 5: An advanced instrument for high spatial resolution studies of particle size spectra and zooplankton ArchiMer
Picheral, Marc; Guidi, Lionel; Stemmann, Lars; Karl, David M.; Iddaoud, Ghizlaine; Gorsky, Gabriel.
The Underwater Vision Profiler (UVP) was developed to quantify the vertical distribution of macroscopic particles and zooplankton > 100 mu m in size. The smaller size limit is fixed by optical resolution, whereas the larger size limit is determined by the volume of water illuminated per image. The new fifth generation instrument (UVP5) is compact (30 kg in air) and operates either as a stand-alone instrument with an independent power supply for use on a mooring or free-drifting array, or as a component of a Conductivity, Temperature, and Depth (CTD)-rosette package. Images are recorded at a frequency up to 6 Hz. If the UVP5 is interfaced with a CTD, these images are acquired and analyzed in real time. Images are recorded every 20 cm at the 1 m s(-1)...
Tipo: Text Palavras-chave: Video plankton recorder; Marine snow; MU-M; Vertical distribution; Aggregate dynamics; In situ; System; Ocean; Flux; Phytoplankton.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00133/24413/22426.pdf
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Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution ArchiMer
Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele.
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of...
Tipo: Text Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf
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Dynamique du phosphore et rôle comme facteur limitant de la production planctonique dans le panache de la Gironde sur le plateau continental Sud Gascogne ArchiMer
Labry, Claire.
This study was supported by the Atlantic working site of the « Programme National d’Océanographie Côtière » (PNOC, PNEC since 1999). The objectives were (1) to check the existence and to understand the mechanisms of winter phytoplankton blooms in the bay of Biscay influenced by the Gironde waters (2) to determine the limiting nutrient of the primary production associated to these blooms and to follow the seasonal evolution of the limiting nutrient, (3) to understand the consequences of this early limitation on the structuration of algal populations in spring. Since phosphorus was supposed to be the major limiting nutrient of algal growth in the Gironde plume, the different forms of the phosphorus cycle as well as the bacterial compartment, central in this...
Tipo: Text Palavras-chave: Golfe de Gascogne; Gironde; Phytoplancton; Bactéries; Phosphates; Phosphore organique; Dissous; Phosphatases; Bay of Biscay; Gironde; Phytoplankton; Bacteria; Phosphate; Dissolved organic phosphorus; Phosphatases.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00013/12463/9298.pdf
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Comparing marine primary production estimates through different methods and development of conversion equations ArchiMer
Regaudie-de-gioux, Aurore; Lasternas, Sébastien; Agustí, Susana; Duarte, Carlos M..
Numerous studies have compared the rates of primary production using various techniques at specific locations and times. However, these comparisons are local and cannot be used to compare or scale rates of primary production using different methods across ocean basins or seasonal time scales. Here, we quantify the range in rates of primary production derived using different techniques and provide equations that allow conversions of estimates between different methods. We do so on the basis of a compilation of data on volumetric estimates of primary production rates concurrently estimated with at least two different methods. We observed that the comparison of estimates of marine phytoplankton primary production derived from different methods reveals very...
Tipo: Text Palavras-chave: Marine; Phytoplankton; GPP; Methods; Conversion.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00420/53120/54072.pdf
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A Bibliometric Analysis of Microalgae Research in the World, Europe, and the European Atlantic Area ArchiMer
Rumin, Judith; Nicolau, Elodie; Gonçalves De Oliveira Junior, Raimundo; Fuentes-grünewald, Claudio; Flynn, Kevin J; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to highlight the evolution of the research domain. Publication rates from 1960 to 2019, organization of the research, collaboration networks between countries and organizations, emerging and fading research concepts, major studied species, and associated concepts, as well as journals publishing microalgae research were considered. As a result, of the 79,020 papers published worldwide, 26,137 included authors from Europe (33% of world...
Tipo: Text Palavras-chave: Biotechnology; European Atlantic Area; Microalgae; Phytoplankton; Research; Market; Bibliometrics.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00605/71725/70191.pdf
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Assessing spatial and temporal variability of phytoplankton communities' composition in the Iroise Sea ecosystem (Brittany, France): A 3D modeling approach. Part 1: Biophysical control over plankton functional types succession and distribution ArchiMer
Cadier, Mathilde; Gorgues, Thomas; Sourisseau, Marc; Edwards, Christopher A.; Aumont, Olivier; Marie, Louis; Memery, Laurent.
Understanding the dynamic interplay between physical, biogeochemical and biological processes represents a key challenge in oceanography, particularly in shelf seas where complex hydrodynamics are likely to drive nutrient distribution and niche partitioning of phytoplankton communities. The Iroise Sea includes a tidal front called the ‘Ushant Front’ that undergoes a pronounced seasonal cycle, with a marked signal during the summer. These characteristics as well as relatively good observational sampling make it a region of choice to study processes impacting phytoplankton dynamics. This innovative modeling study employs a phytoplankton-diversity model, coupled to a regional circulation model to explore mechanisms that alter biogeography of phytoplankton in...
Tipo: Text Palavras-chave: Iroise Sea; Tidal mixing front; Biogeochemical modeling; Phytoplankton; Functional groups; Seasonal cycle.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00352/46367/45986.pdf
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Drivers and ecological consequences of dominance in periurban phytoplankton communities using networks approaches ArchiMer
Escalas, Arthur; Catherine, Arnaud; Maloufi, Selma; Cellamre, Maria; Hamlaoui, Sahima; Yepremian, Claude; Louvard, Clarisse; Trousselier, Marc; Bernard, Cécile.
Evaluating the causes and consequences of dominance by a limited number of taxa in phytoplankton communities is of huge importance in the current context of increasing anthropogenic pressures on natural ecosystems. This is of particular concern in densely populated urban areas where usages and impacts of human populations on water ecosystems are strongly interconnected. Microbial biodiversity is commonly used as a bioindicator of environmental quality and ecosystem functioning, but there are few studies at the regional scale that integrate the drivers of dominance in phytoplankton communities and their consequences on the structure and functioning of these communities. Here, we studied the causes and consequences of phytoplankton dominance in 50...
Tipo: Text Palavras-chave: Dominance; Phytoplankton; Co-occurrence network; Community cohesion; Community functioning; Periurban waterbodies.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00508/61962/66081.pdf
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Suivi régional des nutriments (S.R.N.) sur le littoral du Nord, du Pas-de-Calais et de la Picardie - Bilan de l'année 2009 ArchiMer
Lefebvre, Alain; Cordier, Remy.
The coastal zone of the Nord- Pas de Calais 1 Picardie showed dysfonctioning patterns of the ecosystem considered to be link to human activities along shores. These results in regular massive development of species, such as the phytoplanktonic seaweed, Phaeocystis sp. which life cycle was partly linked to nutrients availability and consequently to anthropogenic inputs. As part of the evaluation of the influence of continental inputs on the marine environment (nitrates, phosphates, ... ) and on potential eutrophication processes, of the estimation of the efficiency of the sewage treatments plants in the possible elimination of dumpings and in order to establish a long-term survey to follow up the change in coastal waters quality, the regional nutrients...
Tipo: Text Palavras-chave: Qualité des eaux littorales; Manche orientale; Baie Sud de la mer du Nord; SurveillanceNutriments; Phytoplancton; Phaeocystis globosa; Quality of coastal waters; Eastern English Channel; Southern bight ofthe North Sea; MonitoringNutrients; Phytoplankton; Phaeocystis globosa.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00082/19363/16957.pdf
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Characterization of Cr. gigas oysters mortalities according to the French Ifremer/REMORA monitoring network ; with complements from phytoplankton and meteorogolical data ArchiMer
Fleury, Pierre-gildas; Mazurie, Joseph; Ropert, Michel; Soletchnik, Patrick; Le Coz, Florence.
The wide range of data from the French Ifremer/REMORA network, collected since 1993 allow a global characterization of mortalities of 1-year old and 2-years old cupped oysters Crassostrea gigas, that are cultivated in the main French oyster areas (Fleury et al, 2003), and thus a better understanding of the causes of these mortalities (Ifremer /MOREST project). The average annual mortalities on intertidal sites range from 10 to 20%. Most of the mortalities take place in Spring and Summer. 1-year oyster mortalities occur in Marennes and several sites in Brittany whilst 2-years oyster mortalities occur in the same sites and in baie des Veys (Normandy). In addition, 2-years oyster mortalities appear more variable according to different annual surveys....
Tipo: Text Palavras-chave: Meteorolgical factors; Phytoplankton; REMORA; Mortalities; Oysters; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4547.pdf
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Dynamics of phytoplankton communities in eutrophying tropical shrimp ponds affected by vibriosis ArchiMer
Lemonnier, Hugues; Lantoine, Francois; Courties, Claude; Guillebault, Delphine; Nezan, Elisabeth; Chomerat, Nicolas; Escoubeyrou, Karine; Galinie, Christian; Blockmans, Bernard; Laugier, Thierry.
Tropical shrimp aquaculture systems in New Caledonia regularly face major crises resulting from outbreaks of Vibrio infections. Ponds are highly dynamic and challenging environments and display a wide range of trophic conditions. In farms affected by vibriosis, phytoplankton biomass and composition are highly variable. These conditions may promote the development of harmful algae increasing shrimp susceptibility to bacterial infections. Phytoplankton compartment before and during mortality outbreaks was monitored at a shrimp farm that has been regularly and highly impacted by these diseases. Combining information from flow cytometry, microscopy, pigment and phylogenetic analysis, the presence of Picocyanobacteria, Prasinophyceae and Diatomophyceae were...
Tipo: Text Palavras-chave: Tropical aquaculture pond; Litopenaeus stylirostris; Vibriosis; Eutrophication; Phytoplankton; Harmful algae.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00343/45411/44986.pdf
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Bilan de l'action de remise en eau du marais de kervijen (Littoral de la Baie de Douarnenez) dans le cadre d'une lutte contre la marée verte. ArchiMer
Piriou, Jean-yves.
ln order to limit the excessive growth of macroalgae Ulva sp. on the beach of Kervijen (Bay of Douarnenez, Brittany, France), a field experiment was carried out, whereby part of the flow of a river was diverted into a coastal marsh. Results showed that the major part of spring and summer, Ulva proliferations have been suppressed. Part of the nitrate transported by river flow was consumed. The coastal marsh produced phytoplankton which regularly flowed on the beach
Tipo: Text Palavras-chave: Marais; Nitrates; Phytoplancton; Ulva sp.; Coastal marsh; Nitrates; Phytoplankton; -U/va sp..
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00132/24299/22297.pdf
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Dinophysis acuminate (dinophyceae), une espèce à géométrie variable sur les côtes françaises ArchiMer
Lassus, Patrick; Marcaillou-le Baut, Claire.
Tipo: Text Palavras-chave: Morphologie; Variabilité; Ultrastructure; Croissance; France; Dinophysis acuminata; Europe; Milieu marin; Zone côtière; Phytoplancton; Pyrrophycophyta; Algae; Thallophyta; Morphology; Variability; Ultrastructure; Growth; France; Europe; Marine environment; Coastal zone; Phytoplankton; Pyrrophycophyta; Algae; Thallophyta.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00133/24468/22484.pdf
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Evolution du phytoplancton dans des bassins de culture ArchiMer
Nival, Paul; Malara, G.; Lelong, P.; Cellario, C.; Charra, R.; Riva, A..
The concentration of chlorophyll a in large out-door continuous cul­ture was analysed in spring 1977 and 1978, and autumn - winter 1977-1978. The growth of marine phytoplankton in springsl977 is analysed with more details and shows three phases : - first, the diatoms (especially Skeletonema costatum) grow rapidely (27/4 to 3/5). The growth rate estimated by the chlorophyll measurements is 1.36 day“1. - then (3/5 to.13/5) the concentration of chlorophyll remains more or less constant (23.611.2 mg/m3) as well as the concentration of particulate organic matter. - at the end of the experiment (13/5 to 20/5) the concentration of chlo­rophyll falls as soon as Skeletonema costatum decrease in number.The declin of the phytoplankton biomass is followed by an...
Tipo: Text Palavras-chave: Phytoplancton; Culture; Croissance; Composition chimique; Espèces; Phytoplankton; Culture; Growth; Chemical composition; Species.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00305/41668/40877.pdf
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Eutrophication in a tropical pond: Understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses ArchiMer
Lucas, Ronan; Courties, C.; Herbland, Alain; Goulletquer, Philippe; Marteau, Anne-laure; Lemonnier, Hugues.
In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4...
Tipo: Text Palavras-chave: Tropical shrimp aquaculture; Ecosystem shift; Phytoplankton; Bacterioplankton; Vibrio; Flow cytometry.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00029/14068/11312.pdf
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Seasonal succession of cyanoprokaryotes in a hypereutrophic oligo-mesohaline lagoon from the South of France ArchiMer
Chomerat, Nicolas; Garnier, Robert; Bertrand, Céline; Cazaubon, Arlette.
The Bolmon lagoon (South of France) is an oligo-mesohaline coastal lagoon that has undergone intense eutrophication in the past decades, resulting from a strong concentration of human activities in its drainage basin. Consequently, it exhibits some characteristics typical of an advanced trophic state; namely, the disappearance of submerged vegetation, the permanently intense phytoplankton growth and the recurrence of cyanoprokaryote blooms. As cyanoprokaryote dominance in south-temperate saline lagoons is little reported, we carried out this study in order to understand the seasonal variations in the phytoplankton composition and biomass, and to analyse the influence of environmental parameters such as salinity, nutrients and climate on the seasonal...
Tipo: Text Palavras-chave: Turbid state; Pseudanabaena limnetica; Planktothrix agardhii; Phytoplankton; Mediterranean area; Cyanoprokaryotes; Ecology; Lagoon; Brackish water.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2593.pdf
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Organisation spatiale et modélisation écologique d'un peuplement phytoplanctonique de lagune (étang de Thau, France) ArchiMer
Jarry, V; Frisoni, Gf; Legendre, P.
A systematic sampling of twenty stations was conducted over four periods within a year (June 1986, October 1986, February 1987, and May 1987) in Thau lagoon. Multivariate analyses (clustering, and principal coordinate analysis) were carried out on phytoplankton species counts. These analyses allowed homogenous areas within the lagoon to be identified and permitted the characterization of these areas in terms of the marine or continental origins of their phytoplankton taxa. The results show the relative importance of each kind of input (marine, continental), and contrast the punctual influence of the continental inputs against the more global effects of the sea. The conclusions drawn from the taxonomic portion of the study were confirmed by statistical...
Tipo: Text Palavras-chave: Phytoplankton; Populations; Chlorophyll pigments; Ecological modelling; Mediterranean lagoon; PHYTOPLANKTON; POPULATIONS; CHLOROPHYLL PIGMENTS; ECOLOGICAL MODELING; MEDITERRANEAN LAGOON.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00323/43397/43105.pdf
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Optimisation de l’identification et du dénombrement du microphytoplancton avec le système couplé de numérisation et d’analyse d’images FlowCAM / ZooPhytoImage (système innovant). Action 9 – Livrable 2. Mise en oeuvre opérationnelle de l’outil FlowCAM-Zoo/PhytoImage dans le cadre de la surveillance REPHY. Résultats sur 12- 18 mois. Rapport final, février 2016 ArchiMer
Neaud-masson, Nadine; Lefebvre, Alain; Belin, Catherine; Gauthier, Emilie; Huguet, Antoine; Szidon, Antoine; Lefebvre, Arnaud; Cuvelliez, Rémi.
This deliverable includes several documents that reflect the operational implementation of the tool FlowCAM / ZooPhytoImage in the framework of REPHY. Among these documents, three reports make an assessment of the entire year 2015, which is not the case with other documents (Master 2 report issued in spring 2015, and minutes of meetings over the year). This summary will be limited to the findings of the three reports providing an updated view of the implementation of the tool. Work completed in 2015 for the operational implementation. The many scans performed in 2015 with the three FlowCAM present at Ifremer (Boulogne‐sur‐Mer, Arcachon and Nantes) allowed, one hand to feed the training sets of the tool FlowCAMZoo/PhytoImage, the other test its routine use....
Tipo: Text Palavras-chave: Phytoplancton; FlowCAM; Zoo/PhytoImage; Numérisation; Analyse d’images; Set d’apprentissage; Outil de reconnaissance; Architecture de données; Bancarisation; Quadrige; Phytoplankton; FlowCAM; Zoo/PhytoImage; Scanning; Image analysis; Training set; Recognition tool; Data architecture; Banking; Quadrige.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47413/47409.pdf
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Pearl Oysters Pinctada margaritifera grazing on natural plankton in Ahe atoll lagoon (Tuamotu archipelago, French Polynesia) ArchiMer
Fournier, Jonathan; Dupuy, Celie; Bouvy, Marc; Couraudon-reale, Marine; Charpy, Loic; Pouvreau, Stephane; Le Moullac, Gilles; Le Pennec, Marie-annick; Cochard, Jean-claude.
In atoll lagoons of French Polynesia, growth and reproduction of pearl oysters are mainly driven by plankton concentration. However, the actual diet of black-lip pearl oysters P. margaritifera in these lagoons is poorly known. To fill this gap, we used the flow through chamber method to measure clearance rates of P. margaritifera in Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia). We found : (i) that pearl oysters cleared plankton at a rate that was positively related to plankton biovolume, (ii) that nanoflagellates were the main source of carbon for the pearl oysters, (iii) that the quantity and origin of carbon filtrated by pearl oysters was highly dependent on the concentration and composition of plankton. These results provide essential...
Tipo: Text Palavras-chave: Pinctada margaritifera; Clearance rates; French Polynesia; Phytoplankton; Protists.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18888/16599.pdf
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