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| Registros recuperados: 148 | |
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| Cavalcante,F.S.; Abad,E.D.; Lyra,Y.C.; Saintive,S.B.; Ribeiro,M.; Ferreira,D.C.; Santos,K.R.N. dos. |
| Staphylococcus aureus is highly prevalent among patients with atopic dermatitis (AD), and this pathogen may trigger and aggravate AD lesions. The aim of this study was to determine the prevalence of S. aureus in the nares of pediatric subjects and verify the phenotypic and molecular characteristics of the isolates in pediatric patients with AD. Isolates were tested for antimicrobial susceptibility, SCCmec typing, and Panton-Valentine Leukocidin (PVL) genes. Lineages were determined by pulsed-field gel electrophoresis and multilocus sequence typing (MLST). AD severity was assessed with the Scoring Atopic Dermatitis (SCORAD) index. Among 106 patients, 90 (85%) presented S. aureus isolates in their nares, and 8 also presented the pathogen in their skin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atopic dermatitis; Staphylococcus aureus; Nasal colonization; Skin lesions; SCORAD. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700588 |
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| Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.. |
| Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071 |
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| Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe. |
| Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310 |
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| Marques,Simone Cristina; Rezende,Jaíne das Graças Oliveira Silva; Alves,Lizandra Aparecida de Freitas; Silva,Belami Cássia; Alves,Eduardo; Abreu,Luiz Ronaldo de; Piccoli,Roberta Hilsdorf. |
| The objectives of this work were to verify the capability of Staphylococcus aureus of forming bio-film on stainless steel and glass surfaces; to evaluate the efficiency of sodium dichloroisocyanurate, hydrogen peroxide and peracetic acid in inactivating Staphylococcus aureus cells adhered onto these surfaces; and to visualize biofilm development by scanning electron microscopy before and after sanitizer treatment. The surfaces studied consisted of 10x20mm chips immersed in Petri dishes containing BHI broth inoculated with S. aureus ATCC 25923. Biofilm formation was observed after 15-day incubation, when the cells were removed using the swab technique, followed by Baird Parker agar plating. Also, the efficiency of the chemical sanitizers on the chip... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm; Stainless steel; Glass; Staphylococcus aureus; Sanitizers; Scanning electron microscopy. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300029 |
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| Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da. |
| The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034 |
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| Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap. |
| This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009 |
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| Souza,Jeana M.E.; Chang,Marilene R.; Brito,Daniela Z.; Farias,Katyuce S.; Damasceno-Junior,Geraldo A.; Turatti,Izabel C.C.; Lopes,Norberto P.; Santos,Edson A.; Carollo,Carlos A.. |
| Abstract We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspilia latissima; Staphylococcus aureus; Bioautographic; MIC; Terpenes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401103 |
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| Registros recuperados: 148 | |
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