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| Registros recuperados: 225 | |
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| Renault, Tristan; Novoa, Beatriz. |
| Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 and 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastern oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses... |
| Tipo: Text |
Palavras-chave: Infection; Virus; Molluscs; Bivalves. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-2910.pdf |
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| Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. |
| To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... |
| Tipo: Text |
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf |
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| Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan. |
| Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982). Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en... |
| Tipo: Text |
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf |
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| Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Grizel, Henri; Berthe, Franck; Cochennec, Nathalie; Renault, Tristan; Le Roux, Frederique. |
| Le réseau REPAMO (Réseau de Pathologie des Mollusques) est un réseau de santé animale, qui s'occupe des coquillages marins du littoral français métropolitain. Les mollusques marins des côtes françaises représentent une ressource par la pêche, mais sont surtout une part importante de l'aquaculture. Les pertes liées aux maladies dans ce domaine d'activité sont aujourd'hui considérées comme l'un des principaux facteurs limitants de son développement au niveau mondial. Deux exemples récents suffisent à illustrer ce danger pour l'ostréiculture française : • La culture de l''huître plate Ostrea edulis, déjà atteinte par un parasite, Marteilia refringens, est passée en quelques années de 20 000 tonnes à 1800 tonnes, suite à une épizootie. En effet à la fin des... |
| Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00098/20903/18522.pdf |
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| Arzul, Isabelle; Renault, Tristan; Lipart, Cecile. |
| Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under... |
| Tipo: Text |
Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf |
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| Renault, Tristan. |
| Although viruses have been associated with mortality outbreaks in different mollusc species, little information is available on their exact affiliation and taxonomic position. Herpes viruses have attracted particular attention because of their economic and ecological impact on both farmed and wild marine molluscs over the past 20 years. Investigations based on molecular approaches have been carried out on the herpes virus infecting Pacific oysters in France. Molecular and ultra-structural data were used to classify the virus under the name ostreid herpesvirus 1 (OsHV-1), as the first known species in the family Malacoherpesviridae. Since 2008, massive mortality outbreaks were reported in several Crassostrea gigas oyster farms in France, Ireland, and the... |
| Tipo: Text |
Palavras-chave: Molluscs; Pacific cupped oyster; Herpes virus; Ostreid herpesvirus 1; Excess mortality. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00069/18000/15562.pdf |
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| Lupo, Coralie; Mandard, Yann-vari; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Renault, Tristan; Bareille, Nathalie. |
| Current monitoring of exceptional mortality events of oysters in France consists in passive surveillance, leading to delayed investigation of causation by scientists and management by policy makers. Early detection of clusters of mortality events in time and space would increase possibilities for their investigation and management. A space-time cluster analysis of existing mortality notifications in Pacific oysters was conducted in France, Charente sluices, from 2008 to 2010. As an example of potential demographic confounder of clustering, the analyses were adjusted on oyster age. Several clusters were identified in space and time each studied year. Patterns of mortality notifications were consistent with the introduction and spread of a contagious disease... |
| Tipo: Text |
Palavras-chave: Notification; Oyster; Mortality; Cluster analysis; Saptiotemporal. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16915/14393.pdf |
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| Soletchnik, Patrick; Goulletquer, Philippe; Cochennec, Nathalie; Renault, Tristan; Geairon, Philippe. |
| A population of Pacific ovsters, Crassostrea gigas, infected with a Chlamydia-like procaryote was reared to compare infection trends under three different feeding conditions: a starved condition, an intermediate condition with a diet of Skeletonema costatllm, and a mixed algal diet. Several physiological and pathological criteriJ: presence of gill abnormality; microscopic examination, basophilic intracytoplasmic inclusions, Feulgen reaction; condition index, filtration, food consumptiQn; fecal production and respiration were used to compare the various modalities one month after the initiation of the experiment. The Irodality of starved oysters showed reduce physiological performances in terms of scope for growth and condition index compared to oysters... |
| Tipo: Text |
Palavras-chave: Pathogen; Chlamydia; Procaryote; Crassostrea gigas; Oyster; Ecophysiology; Pathogène; Chlamydia; Procaryote; Crassostrea gigas; Huître; Ecophysiologie. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-2741.pdf |
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| Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude. |
| Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER. L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités... |
| Tipo: Text |
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf |
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| Grizel, Henri; Renault, Tristan; Cochennec, Nathalie; Tige, Gilbert; Audic, Guylaine; Pichot, Yves; Chollet, Bruno; Goulletquer, Philippe; Soletchnik, Patrick; Geairon, Philippe; Prou, Jean. |
| Le cheptel d'huîtres creuses, Crassostrea gigas, du Bassin de Marennes-Oléron a subi ces dernières années des morta lités (mai 88, mai 91, été 1992 et hiver 92-93). Les examens histopathologiques pratiqués durant celles-ci n'ont pas permis de déceler d'agents pathogènes. Les causes avaient été attribuées essentiellement, pour les premiers cas, à un manque de nourriture corrélé avec des conditions de sécheresse (donc un manque d'apport d'eau douce pour le bassin) et à des surcharges trop importantes des biomasses de fiItreurs, huîtres y compris. L'explication du dernier cas de mortalité survenue au cours de l'hiver serait d'un autre ordre: l'abaissement brutal des salinités, notamment dans les claires où étaient épurées soit des huîtres natives fragilisées,... |
| Tipo: Text |
Palavras-chave: Etude zoosanitaire; Mortalité; Epidémiologie; Pathologie; Marennes Oléron. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00050/16152/13635.pdf |
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| Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.. |
| The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase... |
| Tipo: Text |
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
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| Bado Nilles, A; Gagnaire, Beatrice; Thomas-guyon, Helene; Le Floch, S; Renault, Tristan. |
| The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of... |
| Tipo: Text |
Palavras-chave: Toxicity; PAHs; Pacific oyster; Spectrophotometry; Flow cytometry; Haemocyte activity. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4696.pdf |
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| Hellio, Claire; Bado Nilles, Anne; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Helene. |
| The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at... |
| Tipo: Text |
Palavras-chave: Prophenoloxidase; Phenoloxidase; Oyster; Immunity; Defence; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2926.pdf |
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| Renault, Tristan. |
| Les travaux concernant le virus de type herpès, observé chez les huîtres, ont été poursuivis en 1995-1996 et ont ainsi permis d'aboutir à la mise au point d'un protocole de purification du virus, malgré l'ensemble des difficultés rencontrées (impossibilité de reproduire le virus sur lignées cellulaires, dépendance vis à vis des professionnels pour l'approvisionnement en matériel infecté et difficulté de planifier les expériences). De ce fait, le point de blocage que représentait l'obtention de particules virales en nombre suffisant pour le développement d'outils de diagnostic fiables et sensibles a été levé. |
| Tipo: Text |
Palavras-chave: Herpès virus; Pathologie; Virus; Mortalité; Huître creuse; Crassostrea gigas. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00050/16153/13636.pdf |
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| Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit. |
| Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie.... |
| Tipo: Text |
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf |
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| Garcia, Celine; Thebault, Anne; Degremont, Lionel; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Ferrand, Sylvie; Kerdudou, Nolwenn; Renault, Tristan. |
| Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15110/12572.pdf |
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| Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F.. |
| In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was... |
| Tipo: Text |
Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf |
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| Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle. |
| Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that... |
| Tipo: Text |
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf |
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| Registros recuperados: 225 | |
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