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| Registros recuperados: 457 | |
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| Maria F. Mulas; Antonella Mandas; Claudia Abete; Sandra Dessi; Mario Pani; Rosa Manconi; Alessandra Mocali; Francesco Paoletti. |
| Cholesterol is an essential constituent of all mammalian cell membranes, and its availability is therefore a prerequisite for cellular growth and other functions. Several lines of evidence are now indicating an association between alterations of cholesterol homeostasis and cell cycle progression in cancer cells. However, the role of cholesterol in cell differentiation is still largely unknown. To begin to address this issue, in this study we examined changes in cholesterol metabolism and in the mRNA levels of proteins involved in cholesterol import and esterification (multi-drug resistance, MDR-3) and acylCoA:cholesterol acyltransferase (ACAT) and cholesterol export (caveolin-1) in Friend virus-induced erythroleukemia cells (MELC), in the absence or in the... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5155/version/1 |
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| Graham K. Steel; Phillippa M. Wiggins. |
| This is an attempt to account for the insolubility and/or aggregation of prions and plaques in terms of a model of water consisting of an equilibrium between high 
density and low density microdomains. Hydrophobic molecules, including proteins, 
accumulate selectively into stable populations, enriched in high density water, at 
charged sites on biopolymers. In enriched high density water, proteins are probably 
partially unfolded and may precipitate out when released. All extracellular matrices 
contain such charged polymers. Prions, which have been shown to accumulate in soils 
and clays containing... |
| Tipo: Manuscript |
Palavras-chave: Microbiology; Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1381/version/1 |
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| Guenther Witzany. |
| Communicative competences enable bacteria to develop, organise and coordinate rich social life with a great variety of behavioral patterns even in which they organise themselves like multicellular organisms. They have existed for almost four billion years and still survive, being part of the most dramatic changes in evolutionary history such as DNA invention, cellular life, invention of nearly all protein types, partial constitution of eukaryotic cells, vertical colonisation of all eukaryotes, high adaptability through horizontal gene transfer and co-operative multispecies colonisation of all ecological niches. Recent research demonstrates that these bacterial competences derive from the aptitude of viruses for natural genome editing.... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology; Evolutionary Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1738/version/2 |
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| Ayse Aksoy; Erol Erduran; Yusuf Gedik. |
| Heparin has an apoptotic effect in addition to its anticoagulant, anti-inflammatory, antihypertensive, and antiproliferative effects. In this study, we detected the effect of heparin on the cell cycle of lymphoblasts by flow cytometry (FCM) and tried to determine which phase in the cell cycle of lymphoblasts contains heparin-sensitive points. Twelve children with newly diagnosed acute lymphoblastic leukemia (ALL) were included in the study. Lymphoblasts were incubated in different levels of heparin concentrations (0, 10, and 20 U/mL) and the percentages of lymphoblasts at each phase of the cell cycle were simultaneously measured by FCM at 0, 1, and 2 hours (h). The percentages of lymphoblasts at the G2/M and S phases were significantly increased in 20 U/mL... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1989/version/1 |
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| Philip Campbell. |
| Presented as part of the session titled "Sharing scientific data: who benefits?" at ESOF (The Euroscience Open Forum) 2008.

Session abstract: Digital datasets—text-based, numeric, audio, video or image-based—form the output of all scientific disciplines. How are these data being made available for sharing? What quality control mechanisms are in place? What kinds of naming conventions, tags, and metadata are in use and how effective are they at helping to manage open data? Who is storing, archiving and curating open data and at which levels? And how is the production and sharing of open data assessed: what processes are in place for crediting scientists for making their raw data... |
| Tipo: Presentation |
Palavras-chave: Genetics & Genomics; Molecular Cell Biology; Bioinformatics. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2176/version/1 |
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| Hugues H. Vasseur. |
| When an experimentalist or a biological mechanism applies an external force onto a cell chemically sticking to its substrate, a reacting "suction" force, due to the slow penetration of the surrounding fluid between the cell and the substrate, opposes to the dissociation. This force can overcome other known adhesive forces when the process is sufficiently violent (typically 10^5^ pN). Its maximal contribution to the total adhesive energy of the cell can then be estimated to 2 10^-3^ J/m2. The physical origin of this effect is quite simple, and it may be compared with that leaning a "suction-cup" against a bathroom wall. We address the consequences of this effect on (i) the separation energy, (ii) the motion of the fluid... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1416/version/1 |
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| Jin Gu. |
| Transcription is the central process of gene regulation. In higher eukaryotes, the transcription of a gene is usually regulated by multiple cis-regulatory regions (CRRs). In different tissues, different transcription factors bind to their cis-regulatory motifs in these CRRs to drive tissue-specific expression patterns of their target genes. By combining the genome-wide gene expression data with the genomic sequence data, we proposed multiple-instance scoring (MIS) method to predict the tissue-specific motifs and the corresponding CRRs. The method is mainly based on the assumption that only a subset of CRRs of the expressed gene should function in the studied tissue. By testing on the simulated datasets and the fly muscle dataset, MIS can identify true... |
| Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Molecular Cell Biology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/4038/version/1 |
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| I. C. Baianu. |
| A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/7046/version/1 |
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| Karla Punkt; Katharina Kandt; Andreas Oberbach; Volker Adams; Igor Buchwalow; Matthias Blueher. |
| Muscle-derived nitric oxide (NO) mediates fundamental physiological actions on skeletal muscle including glucose uptake into muscle cells. Recently, we have shown that the altered glucose metabolism in skeletal muscle of patients with type 2 diabetes (T2D) is associated with changes in the metabolic profile of individual muscle fibres, but fibre-type specific changes in NO synthase (NOS) expression in skeletal muscle of T2D patients remain to be elucidated. Here we investigated fibre-type related NOS expression in vastus lateralis muscle of T2D patients compared with healthy individuals with normal glucose tolerance (NGT). Cytophotometrical assay and Western blotting did not reveal any quantitative differences between NOS expression in muscles from NGT and... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/7049/version/1 |
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| Yamini Singh Sisodia; Vivek Bajpai; Deeksha Gaur; Pankaj Kumar Jain. |
| The present study was carried out to systematically examine the pathogenic microorganisms in sewage samples isolated from the Shekhawati region of Rajasthan. In this survey, 26 bacterial strains were isolated and identified using biochemical and molecular methods. Molecular identification of isolates was done by DNA isolation, PCR amplification using 16S rDNA primer and partial sequencing of purified product after sequencing, out of 26, 10 sequences were submitted to NCBI. Out of these 10 isolates Bacillus pumilis, Bacillus subtilis and Bacillus licheniformis were taken for further studies. There is growing awareness of the need for development of new antimicrobial agents for the treatment of human, animal and plant diseases. Keeping this fact in mind, we... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/7073/version/1 |
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| Boris Cvek; Zdenek Dvorak; Jan Taraba; Lukas Muller; Radim Vrzal; Jitka Ulrichova. |
| Inhibiting nuclear factor-kappaB (NF-kB) activation in anticancer and antiinflammatory therapy is of topical interest. Current research in molecular biology has dramatically advanced in the understanding of the cellular events involved in NF-kB induction. Dithiocarbamates, in particular diethyldithiocarbamate and pyrrolidinedithiocarbamate, have been known and widely used as strong inhibitors of NF-kB signaling pathway for more than ten years. Their activity is frequently thought to be due to chelating of zinc or copper present in serum supplemented in the culture medium. Zinc(II) diethyldithiocarbamate (Et2Zn) and zinc(II) dibenzyldithiocarbamate (Bz2Zn) were prepared by direct synthesis in aqueous millieu. They were structurally characterized by X-ray... |
| Tipo: Poster |
Palavras-chave: Cancer; Chemistry; Molecular Cell Biology; Pharmacology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/735/version/1 |
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| Kenji Ohe; Toshiaki Utsumi; Akila Mayeda. |
| The well known transcription factor, HMGA1a, also known as an oncogene, causes aberrant splicing of Presenilin-2 found in sporadic Alzheimer's disease, through sequence specific RNA binding. Since HMGA1 protein levels correlate with tumorigenic malignancy, we are interested in the potential of HMGA1a as an aberrant splicing factor in cancer. The N-terminal truncated isoform of Estrogen Receptor alpha, ERα46, is known to increase expression when breast cancer cells (MCF-7) become hyperconfluent. Transiently expressed HMGA1a induced ERα46 mRNA expression. Plasmid driven micro RNA containing the presenilin-2 HMGA1a RNA binding site and a micro RNA nuclear localization signal (miR_HMGBS_NLS), inhibited ERα46 mRNA... |
| Tipo: Poster |
Palavras-chave: Cancer; Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4104/version/2 |
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| Registros recuperados: 457 | |
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