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| Registros recuperados: 171 | |
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| Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
| The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
| Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
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| Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.. |
| Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into... |
| Tipo: Text |
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf |
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| Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene. |
| Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf |
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| Charles, Erwan; Paquotte, Philippe. |
| France is one of the world top producers of oysters and mussels by aquaculture with 140 000 tonnes of oysters, 60 000 tonnes of mussels and an ex-farm turnover accounting to 350 millions US$ in 1996. The French market is by far the largest one in Europe for oyster and is supplied entirely by domestic production, with very little external trade. On the contrary, the French market for mussel is a very competitive one, widely open to imports. Given the evolution of market conditions due to the development of supermarkets, to the competition of new value added products and to the modification of consumer's habits, the French oyster and mussel producers have started to implement various strategies of product differentiation and of quality approach. These... |
| Tipo: Text |
Palavras-chave: Seafood market study; Product differentiation; Quality; Oyster; Aquaculture; SEM. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-2523.pdf |
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| Bachere, Evelyne; Ifremer, Unité de Pathologie, Immunologie et Génétique Moléculaire des Invertébrés Marins, F-17390 La Tremblade, France; CEV, Centro Experimental de Vilaxoan, Conselleria de Pesca, Villagarcia de Arosa, Spain; CSIC, Consejo Superior Investigaciones Cientificas, Peira de Bouzas S/N, Avda E. Cabello 636208, Vigo, Spain; UCC, University College Cork, Dept of Zoology Cork, Ireland; FCR, Fisheries Research Center, Abbotstown, Castleknock, Dublin 15, Ireland; US, University of Southampton, Dept of Oceanography Southampton S09 5NH, United Kingdom. |
| Bonamia ostreae, an intrahemocytic protozoan parasite infecting the oyster Ostrea edulis, is responsible for an endemic disease affecting all intensive breeding areas of European countries. Epidemiological studies showed variable prevalences, 43% in Galicia (Spain), from 6% to 32% in France and 45% in the most important area of Ireland. The impact of oyster transfers, handlings, high densities have been implicated in the disease development but environmental stressors could be more involved than zootechnical methods. The effect of water temperature, salinity and tidal exposure were particulary examined to assess stress induced changes and determine physiological, biochemical and immunological state indices of oyster populations, which will contribute to... |
| Tipo: Text |
Palavras-chave: Disease; Oyster; Ostrea edulis; Parasitology; Bonamia ostreae; Epidemiology. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00273/38383/36713.pdf |
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| Arnaud, Christophe; Faury, Nicole; Bouquet, Jean-paul; Guesdon, Stephane. |
| Monitoring of reproduction in the oyster Crassostrea gigas, and more particularly in the mussel Mytilis edulis were conducted, in the Marennes-Oléron Bay each year, from June to September. This report gives the main results obtained in 1997, 1998, 1999,2000 and compares the results between years. It also summarises the fishing techniques used and gives information on larval development in the annexes. NOT CONTROLLED OCR |
| Tipo: Text |
Palavras-chave: Marennes Oléron; Larvae; Reproduction; Mussel; Oyster; Marennes Oléron; Larves; Reproduction; Moule; Huître. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/rapport-3883.pdf |
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| Soletchnik, Patrick; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe. |
| The study of maturation and spawning of the oyster is part of a research program to investigate the summer mortalities of the oysters, Crassostrea gigas in Marennes-Oléron Bay. Four maturity stages were simultaneously obtained by diet and thermal conditioning (immature, low maturation, mature and post-spawning stages). Measurements of clearance, filtration, absorption and respiration rates allowed a calculation of the scope for growth and hence an estimation of the oyster's energetic budget at various maturity stages. Male and female oysters had similar physiological responses. The filtration rate ranged from 2.4 to 2.6 1.h(-1) at the early stages of maturation and decreased to 1.8 1.h.' during the maturity stage. Growth rate resulting from gonad... |
| Tipo: Text |
Palavras-chave: Huître; Crassostrea gigas; Conditionnement; Maturation; Filtration; Absorption; Respiration; Oyster; Crassostrea gigas; Conditioning; Maturation; Filtration; Absorption and respiration rates. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/publication-3090.pdf |
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| Renault, Tristan; Cochennec, Nathalie; Grizel, Henri. |
| The protozoan Bonamia ostreae (Ascetospora) is the etiological agent of bonamiosis, or hemocyte disease of the European fiat oyster, Ostrea edulis (Pichot et al., 1980). Since this infection was first reported from the coast of Brittany (Comps et al., 1980), the parasite has spread rapidly along the French Atlantic coasts (Grizel & Tigé, 1982), and in the European countries including the UK, Holland, lreland and Spain (Bannister & Key, 1982; Van Banning, 1982; Polanco et al., 1984). Under the EEC Council Directive 91/67, this parasitic protozoan is included in list II of Annex A. Serious pathogen agents for molluscs that should be obligatorily declared are placed in this list |
| Tipo: Text |
Palavras-chave: Infection; Protozoan; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/1995/publication-2892.pdf |
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| Akcha, Farida; Spagnol, Charlene; Rouxel, Julien. |
| We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with... |
| Tipo: Text |
Palavras-chave: Genotoxicity; Embryotoxicity; Oyster; Gametes; Pesticides; Diuron; Glyphosate. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00070/18128/15783.pdf |
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| Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Truquet, Philippe; Haberkorn, Hansy; Lassus, Patrick; Barille, Laurent; Amzil, Zouher. |
| The aim of this study was to evaluate the importance of biotransformation of paralytic shellfish toxins during the detoxification process in contaminated oysters. Mathematical models based upon the detoxification patterns of digestive gland and other tissues were developed. It was demonstrated that biotransformations do not seem to play an important role in digestive gland or other tissue detoxification kinetics with our data set. Moreover, different toxin transfers from digestive gland toward other tissues were investigated. No significant transfer was highlighted in our data set. These first conclusions were drawn after comparing the results obtained from 13 biotransformations and identifiable transfer scenarios. Finally, to determine a more robust... |
| Tipo: Text |
Palavras-chave: Detoxification kinetics modelling; Biotransformation of saxitoxin analogues; Oyster. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00049/16047/14309.pdf |
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| Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene. |
| Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf |
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| Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan. |
| OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves... |
| Tipo: Text |
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf |
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| Costantini, V; Loisy, Fabienne; Joens, L; Le Guyader, Soizick; Saif, L. |
| Food-borne diseases are a major cause of morbidity and hospitalization worldwide. Enteric caliciviruses are capable of persisting in the environment and in the tissues of shellfish. Human noroviruses (HuNoVs) have been implicated in outbreaks linked to shellfish consumption. The genetic and antigenic relatedness between human and animal enteric calliciviruses suggests that interspecies transmission may occur. To determine the occurrence of human and animal enteric calliciviruses in United States market oysters, we surveyed regional markets. Oysters were collected from 45 bays along the United States coast during the summer and winter of 2002 and 2003. Samples were analyzed by reverse transcription-PCR, and results were confirmed by hybridization and... |
| Tipo: Text |
Palavras-chave: Monitoring; Microbiology; Sampling; Oyster; Shellfish tissue; Human norovirus; Calicivirus. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1235.pdf |
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| Langdon, Cj; Seguineau, Catherine; Ponce, B; Moal, Jeanne; Samain, Jean-francois. |
| Lipid spray beads (SB) were prepared containing 13% w/w particulate riboflavin. Beads suspended in seawater lost 73% riboflavin after 24 h. Release of riboflavin from SE ingested by Pacific oyster (Crassostrea gigas) larvae was observed under epifluorescent light. Riboflavin concentrations in tissues of adult oysters fed on riboflavin-SB were significantly (SNK, P < 0.05) greater than those of oysters fed on seawater-filled SE. Concentrations of riboflavin in oysters exposed to dissolved riboflavin were not significantly greater than those of oysters fed on seawater-filled SE: indicating that elevated riboflavin concentrations in oysters fed on riboflavin-SB were attributable to breakdown of ingested beads rather than uptake of dissolved riboflavin... |
| Tipo: Text |
Palavras-chave: Spray beads; Lipid; Riboflavin; Oyster; Larvae; Crassostrea gigas. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10493/9511.pdf |
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| Renault, Tristan; Arzul, Isabelle. |
| Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf |
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| Registros recuperados: 171 | |
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