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Registros recuperados: 273
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Point sur le programme huître plate - Complément au compte-rendu de réunion REGEMO sur l'Amélioration Génétique de l'huître plate du 11 mars 1998 ArchiMer
Goyard, Emmanuel; Lapegue, Sylvie; Gerard, Andre; Boudry, Pierre.
Ce document vient compéter le rapport REGEMO sur l'huître plate. Une première version visait essentiellement à faciliter le transfert de responsabilité du programme en cours. Celle-ci, épurée des aspects pratiques purement internes, se propose de faire le point sur les familles produites en 1998, et la continuité du programme d'amélioration.
Tipo: Text Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00049/15980/13422.pdf
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Gill Development and Its Functional and Evolutionary Implications in the Blue Mussel Mytilus edulis (Bivalvia: Mytilidae) ArchiMer
Cannuel, Rozenn; Beninger, Peter G.; Mccombie, Helen; Boudry, Pierre.
Study of gill development in bivalve larvae and postlarvae provides information on the evolution of this organ and feeding mechanisms of early stages. Scanning electron microscopy was used to document the development of the filibranch homorhabdic gill in hatchery-reared larval, postlarval, and juvenile Mytilus edulis. Four key stages were identified during gill development: (1) transfer of the particle collection function from velum to gill at metamorphosis, with subsequent elongation of the gill filaments to form a gill basket, with complete frontal ciliation; (2) reflection of the inner demibranchs, and transition to a V-shaped gill; (3) delayed development of the outer demibranchs, occuring simultaneously along the gill axis, with transition to the...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6935.pdf
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Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe ArchiMer
Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf
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Evidence of response to unintentional selection for faster development and inbreeding depression in Crassostrea gigas larvae ArchiMer
Taris, Nicolas; Batista, F; Boudry, Pierre.
Underlying consequences of domestication and artificial selection still remain largely unexplored in most aquacultured species. For species with a two phase life cycle, including the Pacific oyster Crassostrea gigas, most genetic studies have focused on the post-metamorphosis juvenile and adult stages, but relatively few considered the larval stage. To assess the consequence of hatchery practices on larval characters, especially growth, we performed a phenotypic study on larval progenies derived from crosses between Pacific oysters from natural beds and farmed Pacific oysters selected for desirable production traits such as rapid growth, for over seven generations. A set of three microsatellite loci was used to compare the genetic variability between the...
Tipo: Text Palavras-chave: Crassostrea gigas; Inbreeding depression; Selection; Domestication; Larval stage.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3848.pdf
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Comparative histological study of gametogenesis in diploid and triploid pacific oysters (Crassostrea gigas) reared in an estuarine farming site in France during the 2003 heatwave ArchiMer
Normand, Julien; Le Pennec, Marcel; Boudry, Pierre.
We compared the temporal dynamics of gametogenesis in diploid and triploid Pacific oysters (Crassostrea gigas) using histology. Oysters were reared in an estuarine farming site in Brittany over 3 years and their gametogenesis was monitored over the exceptionally hot summer of 2003. Both diploids and triploids showed active gametogenesis, but a high proportion of triploids remained at early stages of gonad development. Gametogenesis of triploids was characterized by the simultaneous occurrence of gonias and immature cytes together with mature gametes rather than overall retardation, though some triploids showed complete gonad maturation. Evidence of spawning was seen in both groups, and the quantity of germinal products emitted appeared to be similar in...
Tipo: Text Palavras-chave: Triploidy; Crassostrea gigas; Oyster; Gametogenesis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4152.pdf
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Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis ArchiMer
Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf
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AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis) ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf
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Genetical basis of the plasticity of resource allocation in the Pacific oyster (Crassostrea gigas) ArchiMer
Ernande, Bruno; Haure, Joel; Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Phenotypic and genetic correlations between fitness-related life-history traits - such as survival, growth and reproductive allocation - have to be considered to better understand selective processes, both in the wild and in breeding programs. Quantitative genetic experiments, based on nested half-sib mating designs, were perfomed in the Pacific oyster C. gigas (1) to estimate genetic variance and the response to selection and (2) to document phenotypic and genetic trade-offs between life history traits. Physiological trade-offs, i.e. plastcity of resource allocation, were shown to vary among genotypes. Genetic polymorphism was primarily observed for the the plasticity of reproductive effort. In addition, the degree of plasticity in reproductive effort...
Tipo: Text Palavras-chave: Genetic polymorphism; Genetic; Plasticity; Genetical basis; Crassostrea gigas; Pacific oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3443.pdf
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Oyster population genetics : understanding natural populations and tracing introductions ArchiMer
Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf
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New insights about the introduction of the Portuguese oyster, Crassostrea angulata, into the North East Atlantic from Asia based on a highly polymorphic mitochondrial region ArchiMer
Grade, Ana; Chairi, Hicham; Lallias, Delphine; Power, Deborah M.; Ruano, Francisco; Leitao, Alexandra; Drago, Teresa; King, Jonathan W.; Boudry, Pierre; Batista, Frederico M..
It is commonly presumed that the Portuguese oyster Crassostrea angulata was introduced into the North East (NE) Atlantic from Asia. The analysis of the nucleotide sequence of a highly polymorphic non-coding mitochondrial region (major noncoding region - MNR) of C. angulata samples collected in Europe (Portugal), Africa (Morocco) and Asia (Shantou and Taiwan) provided new insight into the introduction of this species into the NE Atlantic. Sixty haplotypes and a nucleotide diversity of 0.0077 were observed in 130 analyzed sequences. Higher nucleotide diversity levels were observed in NE Atlantic sites than in Asian sites and significant genetic differentiation was found between the two. Our results suggest that C. angulata might have been introduced to the...
Tipo: Text Palavras-chave: Crassostrea angulata; Phylogeography; Genetic signature; Biological invasions.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00371/48200/48316.pdf
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Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas ArchiMer
Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids. Tetraploid oysters with abnormal chromosome numbers in some or all of their tissue are considered undesirable as parents either for triploids (produced via a cross with diploids and of aquacultural interest) or for tetraploid breeding. Aneuploid tetraploid oysters may confer the tendency to lose chromosomes and to revert to lower ploidy levels to their offspring. More directly, their offspring could have a lower ploidy because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in six bi-parental tetraploid families bred from parents of differing somatic ploidy quality. The...
Tipo: Text Palavras-chave: Crassostrea gigas; Mitosis; Chromosome; Cytogenetics; Aneuploid; Polyptoid; Tetraploid; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-633.pdf
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Effects of hydrodynamic factors on Pecten maximus larval development ArchiMer
Holbach, Marine; Robert, Rene; Miner, Philippe; Mingant, Christian; Boudry, Pierre; Tremblay, Rejean.
Hatchery production of great scallop, Pecten maximus, remains unpredictable, notably due to poor larval survival. Large-scale flow-through systems up to 3500 L have been developed to avoid the use of antibiotics in static systems. Alternatively, small-scale flow-through systems have been successfully applied for oysters but they proved to be unsuitable to rear scallop larvae. By focusing on physical factors presumed to limit P. maximus larval development, this study aimed to optimize great scallop larvae rearing parameters under controlled conditions. First, the influence of aeration on larval performances, energetic metabolism and antioxidant defences were studied both in static and flow-through systems. Aeration depressed larval food intake, regardless...
Tipo: Text Palavras-chave: Aeration; Flow-through; Larval physiology; Pecten maximus.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00384/49508/51151.pdf
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Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet ArchiMer
Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol...
Tipo: Text Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf
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Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins ArchiMer
Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre.
Respiration rates of various geographical strains of Crassostrea gigas were compared to assess the respiratory expenditure as a physiological indicator of catabolism (cost of maintenance). Parental oysters, sampled in France (Marennes-Oleron), Japan (Hiroshima), Taiwan (Tunkang) and Spain (Cadix), were differentiated by both their geographic origin and by mitochondrial DNA markers, allowing the distinction between the two closely related taxa Crassostrea gigas and Crassostrea angulata. After reproduction of these parental oysters, respiration rates of spat of each strain, reared under common controlled conditions, were individually estimated at 20 degrees C by using a volumetric microrespirometer. Our results demonstrated that physiological variability...
Tipo: Text Palavras-chave: Pacific Ocean; NE Atlantic; Oyster strains; Hybrids; Crassostrea angulata; Crassostrea gigas; Metabolism; Respiration rates; Océan Pacifique; Atlantique NE; Souches génétiques; Hybrides; Crassostrea angulata; Crassostrea gigas; Métabolisme; Taux de respiration.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-859.pdf
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Bases génétiques et plasticité de la croissance et de la survie chez l'huître creuse, Crassostrea gigas ArchiMer
Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Growth and survival are the two most interesting characters for oyster farming as they determine the biomass produced in the farms (growth x survival). Thus, a selection program aiming at improving these two characters could be of a great help. However, the oyster, as a sessile organism, is influenced by environmental variability (spatial and temporal) either natural or caused by the used oyster culture methods. Submitted to this variability, oysters must have evolved so as to adapt (short term) to this environmental variability.
Tipo: Text Palavras-chave: Crassostrea gigas; Huîtres; Croissance; Plasticité; Génétique.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3279.pdf
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Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints ArchiMer
Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf
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Etude des processus de dérive et de sélection liés aux pratiques d'élevage en écloserie d'huître creuse ArchiMer
Boudry, Pierre.
Genetic consequences of production of Pacific oyster larval in hatchery: drift and selective pressures related to rearing practices. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors were examined: the effects of discarding the smallest larvae (i.e. culling) and temperature effects. A mixed-family approach was used in order to infer the genetic composition of the larval population. The results show that high polymorphic microsatellite-based family assignment is a powerful tool for the study of bivalve larvae genetics. Culling by selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss if parentage assignment is not introduced as a...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-1459.pdf
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Taxonomie de l'huître naine Ostreola stentina ArchiMer
Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre.
Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view....
Tipo: Text Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf
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FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax) ArchiMer
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010
Registros recuperados: 273
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