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| Registros recuperados: 225 | |
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| Bado-nilles, Anne; Quentin, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Auffret, Michel; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène. |
| Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune... |
| Tipo: Text |
Palavras-chave: PaHs; Fish; Contamination; Dicentrarchus labrax. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3427.pdf |
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| Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe. |
| Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En... |
| Tipo: Text |
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf |
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| Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick. |
| This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf |
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| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
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| Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier. |
| Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf |
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| Renault, Tristan. |
| Depuis 1991 , des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En... |
| Tipo: Text |
Palavras-chave: Mollusques bivalves; Pathologie; Mortalités; Herpes virus; Herpesviridae. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00166/27715/25910.pdf |
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| Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra. |
| Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid... |
| Tipo: Text |
Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00215/32581/35759.pdf |
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| Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N. |
| Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were... |
| Tipo: Text |
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf |
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| Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan. |
| In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes... |
| Tipo: Text |
Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf |
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| Burge, Colleen A.; Friedman, Carolyn S.; Getchell, Rodman; House, Marcia; Lafferty, Kevin D.; Mydlarz, Laura D.; Prager, Katherine C.; Sutherland, Kathryn P.; Renault, Tristan; Kiryu, Ikunari; Vega-thurber, Rebecca. |
| Linking marine epizootics to a specific etiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative realtime PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease... |
| Tipo: Text |
Palavras-chave: Marine disease; Aetiology; Diagnostics; Marine epizootics. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00314/42536/41911.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Arzul, Isabelle. |
| Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line... |
| Tipo: Text |
Palavras-chave: Protozoan; Ostrea edulis; Flat oyster; Transcriptomic; Gene expression. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18218/15790.pdf |
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| Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian. |
| Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf |
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| Renault, Tristan. |
| Global mollusc production is continuously increasing and reached 12.8 million tonnes in 2004 representing 28.2 percent of the total world production valued at US$ 9.4 billions. However, cultivated molluscs may suffer from severe mortality outbreaks. Among the possible causes is the occurrence of infectious diseases due to a variety of pathogens including protozoan parasites, bacteria and viruses. Preventing and controlling diseases has become a priority for aquaculture sustainability. However, molluscs are unique in terms of health management. Molluscs like other invertebrates, lack a true adaptative immune system. Vaccination can not be used to protect them against pathogens. Moreover, molluscs are usually reared in the open sea which strongly limits the... |
| Tipo: Text |
Palavras-chave: Diagnosis; Phylogeny; Genomics; Mollluscs; Pathogens. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4574.pdf |
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| Thebault, Anne; Robert, Maeva; Renault, Tristan; Dumais, M.; Goulletquer, Philippe. |
| Après le naufrage de l'Erika, la question s'est rapidement posée de l'impact des hydrocarbures sur la santé des coquillages, car la pollution concernait des zones de production conchylicoles. Le suivi zoosanitaire en place dans ces zones permettait d'avoir des données de référence avant impact. Il a été choisi différents sites exposés de référence, qui ont fait l'objet d'analyse en toxicologie et en pathologie. A la différence du cas de l'Arnoco Cadiz, l'effort n'a pas été mis uniquement sur les huîtres mais aussi sur d'autres espèces de coquillages, comme les moules et des fouisseurs, particulièrement exposés comme les coques et les palourdes. La mesure de l'exposition effectuée par la Direction de l'Environnement Littoral (MONERlKA) montre qu'une... |
| Tipo: Text |
Palavras-chave: Pollution; Pathologie; Hydrocarbures; Coquillages; HAP; Histologie; Finistère; Vendée. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00087/19826/17468.pdf |
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| Lupo, Coralie; Osta-amigo, Axel; Mandard, Yann-vari; Peroz, Carole; Renault, Tristan. |
| Farmers’ vigilance is essential for the detection of epidemics, including potential emerging diseases, in marine shellfish. A field study was conducted to investigate oyster farmers’ reporting practices and behaviour, and to identify factors influencing the reporting process of oyster mortality, with the ultimate aim of improving early detection of unexplained oyster mortality outbreaks. A retrospective case-control study of oyster farmers from Charente-Maritime (France) was designed, based on interviews with 27 non-reporting and 89 reporting farmers, further split into 40 formerly-reporting and 49 currently-reporting farmers. Information about farmer and farm characteristics, farming practices, farm health history and related financial compensation on... |
| Tipo: Text |
Palavras-chave: Case-control study; Ordinal logistic regression; Surveillance; Notification; Shellfish diseases. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30132/28594.pdf |
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| Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie. |
| The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days.... |
| Tipo: Text |
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf |
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| Registros recuperados: 225 | |
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