|
|
|
| Registros recuperados: 237 | |
|
|
| MENDES,Luiz Cláudio Nogueira; MARQUES,Luiz Carlos; SCHOCKEN-ITURRINO,Ruben Pablo; ÁVILA,Fernando Antônio de; MALHEIROS,Euclides Braga. |
| Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100 x 10(7) colony-forming units (CFU) of E. coli diluted in 500 ml of 0.9% saline; Group II, 100 x 10(7) CFU of Bacteroides fragilis in 500 ml of 0.9% saline; Group III, 100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500 ml of 0.9% saline; Group IV, 500 ml of 0.9% saline. Leukopenia appeared in all animals inoculated with bacteria within the first six hours of the experiment. After this period, leukocytosis was observed in some inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis demonstrated mild... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200011 |
| |
|
|
| Ito,Carmen Antonia Sanches; Gales,Ana Cristina; Tognim,Maria Cristina B.; Munerato,Patrícia; Dalla Costa,Libera Maria. |
| Quinolones (nalidixic acid - NAL, norfloxacin - NOR, ciprofloxacin - CIP and gatifloxacin - GAT) were tested against Escherichia coli isolated from urine (385 patient samples) by disk diffusion (DD) and agar dilution (AD) methods. Fifty-three samples (13.8%) were classified as resistant to at least one of the quinolones tested. CIP and NOR susceptibilities were the same (91.4%) and they were similar to GAT (92.7%). Susceptibility to NAL, detected by the disk diffusion method, was used to predict susceptibility to NOR, CIP and GAT by the agar dilution method. The sensitivity and specificity of NAL were 100% and 95%, respectively. Twelve samples were analyzed for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Urinary infection; Quinolone resistance; Escherichia coli. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100003 |
| |
|
|
| Rocha,Jaime L.; Tuon,Felipe Francisco; Johnson,James R.. |
| BACKGROUND: Optimal empirical therapy of urinary tract infection requires accurate knowledge of local susceptibility patterns, which may vary with organism and patient characteristics. METHODS: Among 9,798 consecutive, non-duplicate, community-source urine isolates from ambulatory patients > 13 years old, from clinical laboratory and an academic medical center in Curitiba, Brazil (May 1st to December 1st, 2009), susceptibility data for ampicillin, nitrofurantoin, trimethoprim-sulfamethoxazole, gentamicin, fluoroquinolones, and ceftriaxone/cefotaxime were compared with organism and patient gender and age. RESULTS: The female-to-male ratio decreased with age, from 28.1 (among 20-29 year-olds) to 3.3 (among > 80 year-olds). Overall, susceptibility... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Urinary tract infections; Anti-bacterial agents; Drug resistance bacterial; Fluoroquinolones; Escherichia coli. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200002 |
| |
|
|
| Pereira,Andrea S.; Andrade,Soraya S.; Monteiro,Jussimara; Sader,Helio S.; Pignatari,Antonio C.C.; Gales,Ana C.. |
| Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quinolone resistance; Escherichia coli; PFGE; Qnr. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100011 |
| |
|
|
| Santo,Edilene; Salvador,Miriam Mendonça; Marin,José Moacir. |
| Multiple resistances to antimicrobial drugs arising in Escherichia coli isolates may complicate therapeutic management of urinary tract infection (UTI) by this organism. In order to assess the multidrug resistance (MDR) among urinary E. coli isolates, we have tested 11 antimicrobial drugs against 67 isolates from outpatients attended in a tertiary-care teaching hospital and of 78 isolates from a municipal health unit, respectively in Ribeirão Preto, State of São Paulo, Brazil. Seventy-six percent and 22% of the isolates from the tertiary-care hospital and the municipal unit, respectively, were resistant to three or more different classes of agents, and were considered to present MDR. Among the isolates from the hospital patients, 73.0%, 65.0%, 58.0%, 58.0%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Urinary tract infections; Escherichia coli; Antimicrobial susceptibility; Multidrug-resistance. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600010 |
| |
|
|
| Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. |
| Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 |
| |
|
|
| Gutiérrez,Camila; Zenis,Javier; Legarraga,Paulette; Cabrera-Pardo,Jaime R.; García,Patricia; Bello-Toledo,Helia; Opazo-Capurro,Andrés; González-Rocha,Gerardo. |
| ABSTRACT Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness of colistin, an antibiotic used as a last-resort treatment against highly antibiotic-resistant bacteria. In 2017, a mcr-1-positive isolate of Escherichia coli was found in Chile for the first time. Herein we report the genetic features of this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 strain belonged to ST4204 and carried a 285 kb IncI2-type plasmid containing the mcr-1 gene. Moreover, this plasmid was transferred by conjugation to an E. coli J53 strain at high frequency. The isolate harbored the cma, iroN, and iss virulence genes and did carry resistance genes to... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Colistin-resistance; Mcr-1; Escherichia coli; Chile. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300203 |
| |
|
| |
|
| |
|
|
| Cuba,Gabriel Trova; Pignatari,Antonio Carlos Campos; Patekoski,Katya Silva; Luchesi,Lucimila Jorge; Kiffer,Carlos Roberto Veiga. |
| Since antimicrobial resistance among uropathogens against current first line agents has affected the management of severe urinary tract infection, we determined the likelihood that antibiotic regimens achieve bactericidal pharmacodynamic exposures using Monte Carlo simulation for five antimicrobials (ciprofloxacin, ceftriaxone, piperacillin/tazobactam, ertapenem, and meropenem) commonly prescribed as initial empirical treatment of inpatients with severe community acquired urinary tract infections. Minimum inhibitory concentration determination by Etest was performed for 205 Brazilian community urinary tract infection Escherichia coli strains from 2008 to 2012 and 74 E. coli bloodstream strains recovered from a surveillance study. Pharmacodynamic exposure... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pharmacodynamics; Monte Carlo method; Escherichia coli; Urinary tract infection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500512 |
| |
|
|
| Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming. |
| ABSTRACT Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates. Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922. Results:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015 |
| |
|
|
| Guidoni,Eliana Biondi Medeiros; Berezin,Eitan N.; Nigro,Stanley; Santiago,Nataly A; Benini,Vanda; Toporovski,Julio. |
| Knowledge about antimicrobial resistance patterns of the etiological agents of urinary tract infections (UTIs) is essential for appropriate therapy. Urinary isolates from symptomatic UTI cases attended at Santa Casa University Hospital of São Paulo from August 1986 to December 1989 and August 2004 to December 2005 were identified by conventional methods. Antimicrobial resistance testing was performed by Kirby Bauer's disc diffusion method. Among the 257 children, E. coli was found in 77%. A high prevalence of resistance was observed against ampicillin and TMP/SMX (55% and 51%). The antibiotic resistance rates for E. coli were: nitrofurantoin (6%), nalidixic acid (14%), 1st generation cephalosporin (13%), 3rd generation cephalosporins (5%), aminoglycosides... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Urinary tract infection; Pediatrics urinary tract infection; Bacterial resistance; Escherichia coli. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400013 |
| |
|
|
| Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.. |
| The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007 |
| |
|
|
| Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.. |
| In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008 |
| |
|
| |
|
| |
|
|
| Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de. |
| Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006 |
| |
|
| |
|
|
| Ananias,M.; Yano,T.. |
| Sixty strains of Escherichia coli, isolated by hemoculture, from septicemic Brazilian patients were evaluated to determine their serogroup and invasivity to Vero cells. All 60 patients died within 2 days of hospitalization. Furthermore, the molecular study of the following extraintestinal pathogenic E. coli-associated virulence factor (VF) genes was performed by PCR: i) adhesins: type 1 fimbria (fimH), S fimbria (sfaD/E), P fimbria (papC and papG alleles) and afimbrial adhesin (afaB/C); ii) capsule K1/K5 (kpsMTII); iii) siderophores: aerobactin (iucD), yersiniabactin (fyuA) and salmochelin (iroN); iv) toxins hemolysin (hlyA), necrotizing cytotoxic factor type 1 (cnf1) and secreted autotransporter toxin (sat); v) miscellaneous: brain microvascular... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Escherichia coli; Sepsis; Serogroups; Virulence genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000008 |
| |
|
|
| Yamamoto,K.; Matsubara,M.; Kawasaki,S.; Bari,M.L.; Kawamoto,S.. |
| Escherichia coli, as a model microorganism, was treated in phosphate-buffered saline under high hydrostatic pressure between 100 and 300 MPa, and the inactivation dynamics was investigated from the viewpoint of predictive microbiology. Inactivation data were curve fitted by typical predictive models: logistic, Gompertz and Weibull functions. Weibull function described the inactivation curve the best. Two parameters of Weibull function were calculated for each holding pressure and their dependence on holding pressure was obtained by interpolation. With the interpolated parameters, inactivation curves were simulated and compared with the experimental data sets. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Predictive microbiology; Pressure; Escherichia coli; Inactivation; Model. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800014 |
| |
| Registros recuperados: 237 | |
|
|
|
