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| Registros recuperados: 181 | |
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| Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar. |
| Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007 |
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| Reichmann,Manuel Castilhos; Zanella,Camila Ângela; Valério Júnior,Cláudio; Borges,Ana Claudia Piovezan; Sausen,Tanise Luisa; Paroul,Natalia; Mielniczki-Pereira,Albanin Aparecida; Teixeira,Amito José; Budke,Jean Carlos; Mossi,Altemir José; Cansian,Rogério Luis. |
| ABSTRACT Understanding the genetic structure and diversity of plants is fundamental to their conservation and permits their sustainable use by local communities. The genus Maytenus (Celastraceae) is composed of plants possessing pharmacological and antioxidant properties. However, the genetic and economic properties of the species M. dasyclada, a typical species of Araucaria forests in Brazil and Uruguay, have been little studied. In this work, the genetic structure and diversity of natural populations of M. dasyclada located in unprotected and preserved forest remnants were investigated using RAPD and isozymes markers. The results demonstrated that in areas of preservation, populations of M. dasyclada possess a relatively high degree of polymorphism and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Forest reserves; Genetic diversity; Maytenus dasyclada; Molecular markers. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100093 |
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| Massucato, Luana Rainieri; Nakamura, Karina Kazue; Ruas, Paulo Mauricio; Zefa, Douglas Mariani; Silva, Derly José Henrique da; Gonçalves, Leandro Simões Azeredo. |
| The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Molecular markers; Phenotype; Bayesian analysis.; Recursos Genéticos. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43426 |
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| Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE. |
| O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas... |
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Palavras-chave: 5.02.01.03-4 Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298 |
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| Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião. |
| GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics.. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361 |
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| Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião. |
| GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics.. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361 |
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| Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari. |
| The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577 |
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| Nunes, Claudinéia Ferreira; Empresa de Pesquisa Agropecuária de Minas Gerais; Ferreira, Juliano Lino; Empresa de Pesquisa Agropecuária de Minas Gerais; Generoso, Andressa Leal; Empresa de Pesquisa Agropecuária de Minas Gerais; Carvalho, Mário Sérgio Dias; Empresa de Pesquisa Agropecuária de Minas Gerais; Pasqual, Moacir; Universidade Federal de Lavras; Cançado, Geraldo Magela de Almeida; Empresa de Pesquisa Agropecuária de Minas Gerais. |
| The strawberry is an important agricultural crop in Brazil. However, most of the commercial genotypes currently in cultivation in Brazil were developed in other countries with environmental adaptations often inadequate for the regional conditions. In this work, inter-simple sequence repeat markers were used to determine the genetic variability and the loci segregation profiles of 84 strawberry hybrids obtained from a genetic breeding program at the ‘Empresa de Pesquisa Agropecuária de Minas Gerais.’ The hybrids were produced from crosses involving the following progenitors: ‘Toyonoka’ x ‘Sweet Charlie’, ‘Camino Real’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Toyonoka’, ‘Dover’ x ‘Oso Grande’, and ‘Camino Real’ x ‘Toyonoka’.... |
| Tipo: Marcadores moleculares |
Palavras-chave: 5.01.03.05-9 genetic dissimilarity; Pre-breeding; Molecular markers Melhoramento Vegetal genetic dissimilarity; Pre-breeding; Molecular markers. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16737 |
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| Almeida-Ferreira, Giovanna Caputo; Cesumar; Oliveira, Alessandra Valéria de; CESUMAR; Prioli, Alberto José; UEM; Prioli, Sônia Maria Alves Pinto; UEM. |
| The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be found in other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti) belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current... |
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Palavras-chave: 2.02.00.00-5 Hybridization; Fish introduction; Molecular markers Genética Hybridization; Fish introduction; Molecular markers. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4855 |
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| BELTRAMINO,ARIEL A.; VOGLER,ROBERTO E.; RUMI,ALEJANDRA; GUZMÁN,LEILA B.; MARTÍN,STELLA M.; PESO,JUANA G.. |
| ABSTRACT The exotic land gastropod Ovachlamys fulgens (Gude, 1900), aka the jumping snail, was registered in Eldorado city, Misiones province, constituting the first documented record of that species in Argentina-and for all of South America as well. Identities of the individuals were confirmed through morphological examination, and by a DNA sequencing of the cytochrome oxidase subunit I gene. DNA sequences for the mitochondrial 16S-rRNA and the nuclear 18S-rRNA genes were also obtained from the morphologically-confirmed specimens to gain insights into the genetic background of this species in Argentina, and as additional markers for enhancing the rapidity of identification by governmental authorities who are responsible for managing the presence of exotic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Argentina; Introduced species; Misiones province; Molecular markers; Nonnative snail. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401591 |
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| SANTOS,DALILHIA N. DOS; FERREIRA,JULIANO L.; SETOTAW,TESFAHUN A.; CANÇADO,GERALDO M.A.; PASQUAL,MOACIR; LONDE,LUCIANA C.N.; SATURNINO,HELOISA M.; VENDRAME,WAGNER A.. |
| ABSTRACT Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Jatropha curcas; Molecular markers; Oilseed crop. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602363 |
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| Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.. |
| The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams & Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012 |
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| Santos,Ronaldo Carvalho dos; Pires,José Luís; Lopes,Uilson Vanderley; Gramacho,Karina Peres G.; Flores,Acassi Batista; Bahia,Rita de Cássia S.; Ramos,Helaine C. Cristine; Corrêa,Ronan Xavier; Ahnert,Dario. |
| Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coefficient of parentage; Molecular markers. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000300005 |
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| Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos. |
| The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinates analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022 |
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| Motta,Ludymila Brandão; Soares,Taís Cristina Bastos; Ferrão,Maria Amélia Gava; Caixeta,Eveline Teixeira; Lorenzoni,Rodrigo Monte; Souza Neto,José Dias de. |
| The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea Arabica; Coffea canephora; Genetic diversity; Molecular markers. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500728 |
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| Registros recuperados: 181 | |
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