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| Registros recuperados: 170 | |
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| Balouet, G.. |
| French summary: In 1974, at the request of a group of oyster farmers in the Brittany region, the Pathology Laboratory of the U.E.R. Medicine of Brest undertook a series of theoretical and applied research projects concerning the problem of flat oyster digestive gland disease, due to Marteilia refringens, the primary economic problem of that time. In 1978, in the research report of Contract C.N.E.X.O. 77/1690, the main results obtained since the beginning of the study of the parasitosis were made public. The present report has as its objective to show: - The results obtained in 1978-1979 while monitoring the parasitosis, and most especially the epidemiological evolution of the disease; - Information concerning the presence of other infectious diseases... |
| Tipo: Text |
Palavras-chave: Parasite; Ostrea edulis; Oyster; Pathology. |
| Ano: 1978 |
URL: http://archimer.ifremer.fr/doc/1978/rapport-1927.pdf |
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| Normand, Julien; Ernande, Bruno; Haure, Joel; Mccombie, Helen; Boudry, Pierre. |
| Early reproductive effort and growth were measured in 3 groups of the Pacific oyster Crassostrea gigas: 1 diploid group and 2 triploid groups resulting from either chemical induction (3nCB) or crosses between tetraploid and diploid parents (3nDT). Oysters were reared under intensive nursery conditions and sampled when 5 mo old. Reproductive effort was estimated by cross-sectional area measurements of the visceral mass (i.e. gonadic occupation) and maturation stage was assessed by qualitative histology. As expected, comparison of the reproductive patterns of these 3 groups revealed a lower reproductive effort in triploid individuals relative to diploid. However, gonadic occupation in triploid oysters was higher than expected in both 3nCB and 3nDT groups, as... |
| Tipo: Text |
Palavras-chave: Triploidy; Oyster; Gametogenesis; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6978.pdf |
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| Durand, Jean-pierre; Goudard, Françoise; Pieri, Jacques; Escoubas, Jean-michel; Schreiber, Nathalie; Cadoret, Jean-paul. |
| Ferritin has been shown as being the principal iron storage in the majority of living organisms. In marine species, ferritin is also involved in high-level accumulation of Po-210. As part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. Ferritin was purified from the visceral mass of the oyster Crassostrea gigas by ion-exchange chromatography and HPLC. SDS-PAGE revealed one band of 20 kDa. An Expressed Sequence Tag (EST) library was screened and led to the identification of two complementary DNA (cDNA) involved in ferritin subunit expression. The complete coding sequences and the untranslated regions (UTRs) of the two genes were obtained and a 5' Rapid... |
| Tipo: Text |
Palavras-chave: Iron; IRE; EST; Oyster. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-693.pdf |
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| Boudry, Pierre; Huvet, Arnaud. |
| The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data... |
| Tipo: Text |
Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf |
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| Debucquet, Gervaise; Cornet, Josiane; Adam, Isabelle; Cardinal, Mireille. |
| The search for new markets in the seafood sector, associated with the question of the continuity of raw oyster consumption over generations can be an opportunity for processors to extend their ranges with oyster-based products. The twofold aim of this study was to evaluate the impact of processing and social representation on perception of oyster-based products by French consumers and to identify the best means of development in order to avoid possible failure in the market. Five products with different degrees of processing (cooked oysters in a half-shell, hot preparation for toast, potted oyster, oyster butter and oyster-based soup) were presented within focus groups and consumer tests, at home and in canteens with the staff of several companies in order... |
| Tipo: Text |
Palavras-chave: Oyster; Processing; Consumer perception; Disgust; Social representation. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20945/18603.pdf |
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| Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne. |
| A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other... |
| Tipo: Text |
Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf |
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| Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P.. |
| The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were... |
| Tipo: Text |
Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf |
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| Langdon, Cj; Seguineau, Catherine; Ponce, B; Moal, Jeanne; Samain, Jean-francois. |
| Lipid spray beads (SB) were prepared containing 13% w/w particulate riboflavin. Beads suspended in seawater lost 73% riboflavin after 24 h. Release of riboflavin from SE ingested by Pacific oyster (Crassostrea gigas) larvae was observed under epifluorescent light. Riboflavin concentrations in tissues of adult oysters fed on riboflavin-SB were significantly (SNK, P < 0.05) greater than those of oysters fed on seawater-filled SE. Concentrations of riboflavin in oysters exposed to dissolved riboflavin were not significantly greater than those of oysters fed on seawater-filled SE: indicating that elevated riboflavin concentrations in oysters fed on riboflavin-SB were attributable to breakdown of ingested beads rather than uptake of dissolved riboflavin... |
| Tipo: Text |
Palavras-chave: Spray beads; Lipid; Riboflavin; Oyster; Larvae; Crassostrea gigas. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10493/9511.pdf |
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| Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. |
| To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... |
| Tipo: Text |
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf |
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| Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre. |
| Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids bred in this species. Tetraploid animals with lower chromosome counts in some or all of their somatic tissue are considered undesirable as parents either for commercial triploids (produced via a cross with diploids) or for tetraploid breeding because (1) they may confer the tendency to lose chromosomes and revert to lower ploidy levels to their offspring or (2) their offspring could have a lower ploidy directly because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in bi-parental tetraploid families bred from parents of differing ploidy quality, including a... |
| Tipo: Text |
Palavras-chave: Tetraploid; Chromosome; Genetic; Crassostrea gigas; Oyster. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3435.pdf |
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| Degremont, Lionel; Garcia, Celine; Allen, Standish K., Jr.. |
| Oyster species suffer from numerous disease outbreaks, often causing high mortality. Because the environment cannot be controlled, genetic improvement for disease resistance to pathogens is an attractive option to reduce their impact on oyster production. We review the literature on selective breeding programs for disease resistance in oyster species, and the impact of triploidy on such resistance. Significant response to selection to improve disease resistance was observed in all studies after two to four generations of selection for Haplosporidium nelsoni and Roseovarius crassostrea in Crassostrea virginica, OsHV-1 in Crassostrea gigas, and Martelia sydneyi in Saccostrea glomerata. Clearly, resistance in these cases was heritable, but most of the studies... |
| Tipo: Text |
Palavras-chave: Disease resistance; Oyster; Selection; Pathogen; Genetic; Mortality. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/38049/36139.pdf |
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| Bachere, Evelyne; Ifremer, Unité de Pathologie, Immunologie et Génétique Moléculaire des Invertébrés Marins, F-17390 La Tremblade, France; CEV, Centro Experimental de Vilaxoan, Conselleria de Pesca, Villagarcia de Arosa, Spain; CSIC, Consejo Superior Investigaciones Cientificas, Peira de Bouzas S/N, Avda E. Cabello 636208, Vigo, Spain; UCC, University College Cork, Dept of Zoology Cork, Ireland; FCR, Fisheries Research Center, Abbotstown, Castleknock, Dublin 15, Ireland; US, University of Southampton, Dept of Oceanography Southampton S09 5NH, United Kingdom. |
| Bonamia ostreae, an intrahemocytic protozoan parasite infecting the oyster Ostrea edulis, is responsible for an endemic disease affecting all intensive breeding areas of European countries. Epidemiological studies showed variable prevalences, 43% in Galicia (Spain), from 6% to 32% in France and 45% in the most important area of Ireland. The impact of oyster transfers, handlings, high densities have been implicated in the disease development but environmental stressors could be more involved than zootechnical methods. The effect of water temperature, salinity and tidal exposure were particulary examined to assess stress induced changes and determine physiological, biochemical and immunological state indices of oyster populations, which will contribute to... |
| Tipo: Text |
Palavras-chave: Disease; Oyster; Ostrea edulis; Parasitology; Bonamia ostreae; Epidemiology. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00273/38383/36713.pdf |
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| Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume. |
| Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval... |
| Tipo: Text |
Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf |
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| Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. |
| The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... |
| Tipo: Text |
Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf |
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| Registros recuperados: 170 | |
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