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Registros recuperados: 225 | |
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Renault, Tristan. |
Among the infectious diseases observed in bivalve molluscs, viral infections are often not well known because of a certain inadequacy of the diagnostic techniques usually applied during instances of mortality. In fact, photonic microscopy was still the base method for analysing specimens in several laboratories working on pathologies in molluscs. This technique is insufficient in the event of a pathology of viral etiology if it is not supplemented by other approaches such as the electronic microscope, research of cytopathogenic effects on cell lines or detection of the infectious agent with the help of specific reagents (specific antibodies and nucleic probes). Moreover, since no bivalve mollusc cell line is currently available, in vitro research of... |
Tipo: Text |
Palavras-chave: Virologie; Mollusques; Détection; Maladie; Herpesviridae. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-2903.pdf |
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Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno. |
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C, virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Marteilia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp, during cohabitation with Marteilia-infected native bivalves in France. |
Tipo: Text |
Palavras-chave: American oyster; Crassostrea virginica; Marteiliosis; Marteilia-like parasite. |
Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00258/36926/36177.pdf |
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Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Renault, Tristan; Cochennec, Nathalie. |
Premier volet du programme d'essai d'acclimatation de l'huître américaine Crassostrea virginica, ce rapport présente les actions et les résultats de l'année 1992 qui a été consacrée à : • l'importation selon les normes du CIEM d'un lot de 50 huîtres en provenance d'Angleterre, • leur élevage en salle de quarantaine et leur conditionnement en vue d'une accélération de la maturation sexuelle, • l'obtention d'une première génération, ainsi que son suivi en élevage larvaire et en micronurserie à l'URGE-La Tremblade, • au contrôle des performances comparées des deux populations de C. virginica et de C. gigas en nurserie de prégrossissement à l'URRA-Bouin pendant toute la période automnale et hivernale. Au terme de cette première année d'étude il apparait que la... |
Tipo: Text |
Palavras-chave: Mollusques; Acclimatation; Crassostrea virginica; Crassostrea gigas; Génétique; Pathologie. |
Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00077/18843/16417.pdf |
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Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan. |
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in... |
Tipo: Text |
Palavras-chave: Intéraction; Hémocytes; Ostrea edulis; Bonamia ostreae; Parasitologie. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3366.pdf |
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Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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Renault, Tristan. |
The world halieutic production reached 133 million tons in 2002. The part of aquaculture in the world halieutic production grew from 25.8% to 29.9% between 1998 and 2002. Over the same period, if the fishing production increased by 6.3%, the aquacultural production progressed by 30%. According to the FAO, the world fish, mollusc and crustacea consumption could be around 179 million tons by 2015. Most of this new demand will have to be satisfied by aquaculture. By 2015, aquaculture could represent 39% of the world halieutic production. Infectious diseases may influence the survival, but also the growth and zootechnical performances of cultivated animals. As a matter of fact, they are hazards that have to be taken into account and mastered. Aquaculture, as... |
Tipo: Text |
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Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/these-3486.pdf |
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Renault, Tristan. |
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années... |
Tipo: Text |
Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf |
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Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie. |
La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en... |
Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance. |
Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf |
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Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan. |
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely... |
Tipo: Text |
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf |
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Renault, Tristan; Lipart, Cecile; Arzul, Isabelle. |
Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters,... |
Tipo: Text |
Palavras-chave: Apoptosis; Virus replication; Mortality; Ruditapes philippinarum; Manila clam; Herpes like virus. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-816.pdf |
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Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry. |
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and... |
Tipo: Text |
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf |
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Katarzyna, S; Renault, Tristan; Wolowicz, M. |
This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of... |
Tipo: Text |
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf |
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Barbosa-solomieu, Valerie; Renault, Tristan; Travers, Marie-agnes. |
Massive mortality outbreaks in cultured bivalves have been reported worldwide and they have been associated with infection by a range of viral and bacterial pathogens. Due to their economic and social impact, these episodes constitute a particularly sensitive issue in Pacific oyster (Crassostrea gigas) production. Since 2008, mortality outbreaks affecting C. gigas have increased in terms of intensity and geographic distribution. Epidemiologic surveys have lead to the incrimination of pathogens, specifically OsHV-1 and bacteria of the Vibrio genus, in particular Vibrio aestuarianus. Pathogen diversity may partially account for the variability in the outcome of infections. Host factors (age, reproductive status…) including their genetic background that has... |
Tipo: Text |
Palavras-chave: Pacific oyster; Spat; OsHV-1; Vibrio aestuarianus; Mortality. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38534/37051.pdf |
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Registros recuperados: 225 | |
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