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| Registros recuperados: 225 | |
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| Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck. |
| Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of... |
| Tipo: Text |
Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf |
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| Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely... |
| Tipo: Text |
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf |
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| Dubois, Rodolphe. |
| Bonamia ostreae, l'agent de la bonamiose, est un parasite intracellulaire ayant pour cible les hémocytes (cellules du système de défense) de l'huître plate ostrea edulis. Il induit chez son hôte des lésions branchiales, telles que des perforations ou des indentations pouvant aller jusqu'à la mort du mollusque. Le travail réalisé au cours de ce stage s'inscrivait dans une démarche de validation de méthode de PCR (Polymerase Chain Reaction) pour la détection de ce parasite. Les analyses ont porté sur un lot de trois cent onze huîtres plates ostrea edulis adultes (2-3 ans), issus de captage naturel et provenant de Quiberon (Bretagne). Pour chaque individu, une extraction d'ADN a été réalisée sur un échantillon de coeur et de branchies. Afin de déterminer si... |
| Tipo: Text |
Palavras-chave: Bonamia Ostreae; Agent pathogène; Bonamiose; Hémocytes; Huître plate; Ostrea edulis; PCR; Validation. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00369/47989/48017.pdf |
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| Tige, Gilbert; Grizel, Henri; Langlade, Aime; Rabouin, Marie-agnès. |
| Les premiers stades connus du parasite ont été mis en évidence en Bretagne pendant les mois de juillet et d'août (GRIZEL et TIGE, 1977). Suite à ces observations, un essai a été réalisé en 1978 pour savoir si l'infestation avait bien lieu à ces dates ou si elle se faisait antérieurement sous une forme non décelée actuellement et subissant des transformations profondes dans les tissus de l'huître. Les résultats montrent que l'infestations se fait effectivement pendant l'été, à partir du mois de juillet jusqu'à la fin du mois d'août. Elle fut dans l'ensemble faible; les lots les plus infestés sont ceux qui ont séjourné le plus longtemps en zone contaminée. |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Marteilia refringens; Pathologie; Parasite. |
| Ano: 1979 |
URL: http://archimer.ifremer.fr/doc/1979/acte-6098.PDF |
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| Marteil, Louis; Trochon, Pierre; Le Dantec, Jean; Moreau, Jean. |
| La présence d'Hexamita chez l'huître a été signalée par Certes dès 1892 mais son rôle dans l'étiologie de certaines maladies n'a été précisé qu'en 1952 par Mackin, Korringa et Hopkins. En Hollande, au Canada, aux U. S. A. ce protozoaire a été jugé responsable de mortalités importantes, survenant particulièrement dans les bassins de stockage, pendant l'hiver: une forte densité de peuplement, un renouvellement insuffisant de l'eau, une basse température étaient considérés comme des facteurs favorables au développement de ce flagellé. |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Crassostrea angulata; Hexamita; Parasite; Infestation. |
| Ano: 1962 |
URL: http://archimer.ifremer.fr/doc/00005/11583/8208.pdf |
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| Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre. |
| During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the... |
| Tipo: Text |
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf |
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| Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge. |
| The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and... |
| Tipo: Text |
Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf |
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| Tige, Gilbert; De Kergariou, Gabriel; Cochennec, Nathalie; Rabouin, Marie-agnès. |
| A systematic surveillance progam conducted since 1980 along the coasts of Britanny has followed the evolution and epidemiological state of the natural O. eulis oyster beds and seed areas in the intertidal zones and deep water. Presently, Marteilia refringens exists endemically in Brittany estuaries ; the rate and degree of infestation varies from year to year depending on the hydrological conditions and the geographical region. In no case, however, is the return of flat oyster culture envisoned. Bonamia ostreae is present not only in the estuaries but also in the deep-water rearing areas (Baie de Quiberon, Cancale, Baie de St Brieuc). The infestation increases with age, but also depends on the quantity of the stock and the culture practises. In particular,... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Maladie; Bonamia ostreae; Marteilia refringens; Bretagne; Oyster; Ostrea edulis; Diseases; Bonamia ostreae; Marteilia refringens. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/00022/13299/10353.pdf |
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| Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois. |
| Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes. |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf |
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| Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy. |
| The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are... |
| Tipo: Text |
Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf |
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| Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle. |
| Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using... |
| Tipo: Text |
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf |
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| Rodriguez, David. |
| Les parasites du genre Marteilia sont responsables de graves épizooties et sont retrouvés dans une communauté très variable de mollusques bivalves marins. Toutefois ces protistes restent difficiles à classer à l'intérieur du genre du fait que les données morphologiques sont insuffisantes pour distinguer avec précision deux espèces données. Ceci est le cas pour Martilia refringens, affectant l'huître plate Ostrea edulis ou la moule (Mytilus edulis et Mytilus galloprovincialis), Marteilia sp. et Marteilia maurini affectant les deux espèces de moules. C'est pourquoi, nous avons tenté d'isoler, par PCR, le gène codant pour l'ARN 18S, les ITS1 et ITS2 ainsi que le gène codant pour l'ARN 5,8S des parasites présents chez l'huître plate et la moule (M. edulis),... |
| Tipo: Text |
Palavras-chave: Parasite; Marteilia; PCR; Ostrea edulis; Mytilus edulis; Biologie moléculaire. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00032/14352/11636.pdf |
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| Goyard, Emmanuel; Lapegue, Sylvie; Cochennec, Nathalie; Baud, Jean-pierre; Langlade, Aime; Joly, Jean-pierre; Buestel, Dominique; Oheix, Jocelyne; Haure, Joel. |
| Le programme de sélection de l'huître plate a été initié en 1985 par des pathologistes de la Tremblade. La sélection ne portait que sur le seul critère de tolérance des huîtres plates au parasite Bonamia ostreae et correspondait plus à un outil pour étudier divers aspects de la parasitose qu'un un programme d'amélioration génétique visant la relance de la culture de l'huître plate. Dans le cadre du réseau REGEMO, ce programme a ensuite été repris par les généticiens de l'Ifremer : 1) rappel historique et définition d'un vocabulaire commun à l'ensemble des membres du réseau REGEMO. 2) Inventaire et bilan des opération menées ou en cours ( sur la dernière génération de sélection produite ne 1995, sur le matériel issu de cette génération et produit en 1997.... |
| Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00049/15977/13419.pdf |
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| Registros recuperados: 225 | |
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