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Development and validation of a method for the detection of altered resistance in transgenic plants against herbivore-pathogen-complexes Nature Precedings
Nadine Drechsler; Antje Habekuß; Thomas Thieme; Jörg Schubert.
Enhanced or reduced uptake of viruses by vectors and changes in resistance level can be sensitive indicators for metabolic changes in transgenic plants caused by the new trait and not observed by conventional methods. In addition, the plant transformation process itself can lead to such changes. To be able to investigate this hypothesis in cereals we decided to use two highly important insect-transmitted viruses infecting them - _Barley yellow dwarf virus_ (BYDV) and _Wheat dwarf virus_ (WDV). Corresponding molecular tools for their quantification in plants as well as virus vectors had to be developed. 
Both viruses cause similar symptoms: dwarfing, stunting, leaf discoloration leading to yield losses or death of plant. BYDV...
Tipo: Poster Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4493/version/1
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Single Cancer Cell Detection by Near Infrared Microspectroscopy, Infrared Chemical Imaging and Fluorescence Microspectroscopy Nature Precedings
I. C. Baianu; Schuyler S. Korban; Doina M. Costescu.
Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier Transform Infrared (FT-IR), Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems approaching 1micron resolution are here reported. 400 and 500 MHz, H-1 NMR analyses were carried out that allowed the selection of mutagenized embryos. Detailed chemical analyses are being demonstrated to be also possible by FT-NIR Chemical Imaging/ Microspectroscopy of single cancer cells. FT-NIR Microspectroscopy and Chemical Imaging are also shown to be potentially important in Functional...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6207/version/1
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Evaluation of resistance in wheat against Rhopalosiphumpadi (L.) (Homoptera:Aphididae) Under laboratory Conditions Nature Precedings
Naheed Akhtar; Noreen Moin; Ghulam Jilani; Ata U. Mohsin; Shaheena Yasmin; Afzala Afzala; Munir Goraya; Atif A. Baig.
Aims and introduction
All over the world aphids are pests of economic importance, affecting all varieties of plants like cereal crops, vegetables, ornamental plants and fruits. Aphids damage plants either directly or indirectly. Aphids are one of the major agricultural pests. There are only a few plants that are not susceptible to infestation by this destructive pest. They cause direct damage by sucking the sap of leaves, blocking photosynthesis which results in leaf distortion, gall production, discoloration, stunting, leaf curling, wilting, twisting and premature leaf falls. They are directly involved in transmission of plant viruses and indirectly by depositing honey dew that reduces photosynthetic activity and induces sooty mold...
Tipo: Presentation Palavras-chave: Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5947/version/1
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Nitrogen Fixation and Translocation in Sugarcane Nature Precedings
Takuji Ohyama; Atsushi Momose; Norikuni Ohtake; Kuni Sueyoshi; Takashi Sato; Yasuhiro Nakanishi; Shoichiro Akao.
World sugarcane production is increasing rapidly as a biofuel. In some areas in Brazil, sugarcane has been grown continually over very long periods without N fertiliser inputs. Therefore, the occurrence of N fixation has been suspected. However, quantitative studies seeking to identify the N~2~ fixation sites in the plant and to record the translocation of fixed N around the plant have not yet established. A ^15^N~2~ gas tracer experiment was conducted using young sugarcane plants to investigate the sites of N~2~ fixation and also to explore the possibility of translocation of the fixed N among the plant's major organs. Young sugarcane plants (_Saccharum officinarum_ L.) about 40 cm high and some 14 days after sprouting from a stem cutting were...
Tipo: Manuscript Palavras-chave: Ecology; Earth & Environment; Plant Biology.
Ano: 2008 URL: http://precedings.nature.com/documents/2360/version/1
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Dust in the wind – drift of dust containing insecticides – a risk for honey bees (_Apis mellifera_ L.)? Nature Precedings
Pablo T. Georgiadis; Jens Pistorius; Udo Heimbach.
In late April 2008, dust drift containing insecticide resulted in the largest bee poisoning in Germany for 30 years. The reason for these incidents was the contamination of flowering bee forage plants with dust particles abraded from maize seeds treated with Clothianidin recorded in the Upper Rhine Valley, Baden-Wuerttemberg and in parts of Bavaria during sowing of maize.

Clothianidin, an insecticidal active substance from the group of neonicotinoids, works as agonist at the nicotinic acetylcholine receptors with broad spectrum of efficacy. Clothianidin is also used as a seed dressing of maize (agent for seed treatment “Poncho Pro®”; 1,.25 mg a.i. Clothianidin./kernel) for the control of the...
Tipo: Presentation Palavras-chave: Chemistry; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5738/version/1
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Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry Nature Precedings
Vishal Gaurav; Amit Shavit; Susan C. Roberts.
Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular metabolism is studied at the genomic level. Genomic stability in plant cell cultures is crucial as it affects cell growth and division, metabolite accumulation and protein synthesis. A rapid and efficient method to prepare nuclei suspensions from aggregated cell cultures of Taxus was employed. Methods were subsequently developed to simultaneously stain them for DNA and protein content using Propidium Iodide and Fluorescein Isothiocyanate respectively. Flow cytometry was used to analyze and quantify the DNA...
Tipo: Poster Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3809/version/1
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The Differences Between Products Of Gene Expression In Male, Female And Hermaphrodite Cucumber Floral Buds (Cucumis Sativus L.) Nature Precedings
Magdalena Pawelkowicz; Ewa Siedlecka; Zbigniew Przybecki.
Cucumber is a species in which sex expression has been extensively studied. Sexual differentiation is controlled by genotypic and environmental factors. The main genes responsible for sex determination have been described but the mechanism of their action remains unexplained. In this study we attempted to find cDNA clones which can be connected with sex differentiation and flower development in cucumber. 
Two pairs of nearly isogenic lines: GY-3 (gynoecious – FFMMGG) and HGY-3 (hermaphrodite - FFmmGG), B10 (monoecious - ffMMGG) and 2gg (gynoecious - FFMMgg) were used to search for differences in gene expression in young (1 – 2mm) cucumber floral buds.
In order to obtain differentially expressed cDNA...
Tipo: Poster Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5605/version/1
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Future Predictions of _Cyclamen_ Distribution in the Mediterranean Region Nature Precedings
Claudia Ciotir; Chris Yesson; Alastair Culham.
_Cyclamen_ (Myrsinaceae) occupy the Mediterranean region, with highest diversity in Greece and Turkey, with a few species endemic to islands. _Cyclamen_ species have a phenological preference for dry summers and wet winters. A recent study developed models of the climatic niches of _Cyclamen_. These models were projected into future climate scenarios for 2050. The area of climatic suitability for every _Cyclamen_ species is predicted to decrease. A limited dispersal capacity places them at high risk of extinction. Half of the species are threat ened with extinction due to their potential area loss, indicating that conservation and protection for these species has to be implemented by adding them to the red list.
Tipo: Poster Palavras-chave: Ecology; Plant Biology; Evolutionary Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3066/version/1
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NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil, Proteins and Water Contents [v.2] Nature Precedings
I. C. Baianu; Jun Guo; Randall L. Nelson; Tiefeng You; Doina M. Costescu.
Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared (NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert and Beer’s law, and quantitative measurements can be successfully made with high speed and ease of operation. NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the food industry...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6611/version/2
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Evaluation of Antimicrobial Studies of Traditional Medicine Nature Precedings
Ramar Perumal Samy; S. Ignacimuthu; Vincent TK Chow.
Synthetic drugs, which are having serious side effects, are cytotoxic to human beings. Moreover, bacteria slowly become resistant against antibiotics. In order to overcome these problems, scientists are continuing to search for antibacterial and novel principles from plants. Zanthoxylum limonella has been popularly used in traditional systems of medicine for the treatment of dental caries, cardiac, respiratory diseases and stomach problems. The present study was therefore focused on finding out the medicinal properties of the bark of Z. limonella tested against some selected bacteria using disc-diffusion method at the concentration of 100, 200 and 300 mg/ml. The methanol and water extracts of the bark exhibited effective antibacterial activity against S....
Tipo: Manuscript Palavras-chave: Microbiology; Pharmacology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6027/version/1
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Strong violation of the competitive exclusion principle Nature Precedings
Lev V. Kalmykov; Vyacheslav L. Kalmykov.
Bacteria and plants are able to form population waves as a result of their consumer behaviour and propagation. A soliton-like interpenetration of colliding population waves was assumed but not proved earlier. Here we show how and why colliding population waves of trophically identical but fitness different species can interpenetrate through each other without delay. We have hypothesized and revealed here that the last mechanism provides a stable coexistence of two, three and four species, competing for the same limiting resource in the small homogeneous habitat under constant conditions and without any fitness trade-offs. We have explained the mystery of biodiversity mechanistically because (i) our models are bottom-up mechanistic, (ii) the revealed...
Tipo: Manuscript Palavras-chave: Biotechnology; Ecology; Microbiology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6667/version/1
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Vermiculiphily: Larvae Pollinating Orchids! Nature Precedings
Pankaj Kumar; Gopal S. Rawat.
Orchids are truly extraordinary organisms exhibiting a wide range of pollination mechanism, many of which awaits discovery. Epipactis veratrifolia is a terrestrial orchid that has been reported to be pollinated by various species of hoverflies in Israel1,2. This orchid is known to emit the smell of aphid pheromone that attracts hoverflies, which lay eggs inside the flower and in the process pollinate them. Recently, we observed a species of hoverfly (Ischiodon scutellaris) pollinate the same orchid species in the Western Himalaya while laying eggs inside the flowers. Ischiodon scutellaris is being reported for the first time as pollinator of this orchid. However, all flowers don’t get pollinated, even though insects visit them and lay eggs...
Tipo: Manuscript Palavras-chave: Ecology; Plant Biology; Evolutionary Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6295/version/1
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Decadal-scale changes in seagrass coverage on the Mississippi barrier islands, northern Gulf of Mexico Nature Precedings
Gregory A. Carter; G. Alan Criss; Gabriel A. Blossom; Patrick D. Biber.
Seagrasses provide habitat for a wide variety of aquatic species, and reduce coastal erosion via sediment stabilization. The areal coverage of seagrass beds on the Mississippi barrier islands appears to have declined substantially since the 1960s. However, difficulties in sampling benthic vegetation, differences among previous studies in survey methods, and a lack of data on year-to-year variation in coverage have impeded quantitative study of this decline. Thus, the goal of the present study was to utilize historical remote sensing data to quantitatively determine changes in seagrass (predominantly Halodule wrightii Ascherson) coverage on the Mississippi barrier islands. Analyses were limited to imagery acquired during late summer through autumn, when...
Tipo: Poster Palavras-chave: Ecology; Earth & Environment; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3669/version/1
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Antioxidant response of overwintering conifers suggest species specific strategies for coping with excess excitation energy Nature Precedings
Kim Hughes; Amy Verhoeven; Alex Kokula.
Background/Question/Methods:
Evergreen plants, growing in seasonally cold environments, must cope with a severe imbalance between light absorption and its utilization, as low temperatures during winter inhibit photosynthetic carbon reduction. In such conditions photoprotective mechanisms are critical, including the leaf antioxidant systems of plants. In a previous study examining _Taxus cuspidata_, we found significant increases in the enzyme glutathione reductase (GR) and the metabolite glutathione (GSH) during winter, but no increases in the enzymes superoxide dismutase, ascorbate peroxidase (APX) or the metabolite ascorbate (ASC). These results led to the hypothesis that GR and GSH serve an important role during winter stress that was...
Tipo: Poster Palavras-chave: Earth & Environment; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/5324/version/1
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The Effects of Climate Change on the Phenological Interactions of Plants and Pollinators Nature Precedings
David W. Inouye.
Symposium title: Interdisciplinary Canary: Linkages between Ecology and Sustainable Decision Making in a Dynamic Environment

*1) Background/Question/Methods*
The responses of pollinators to climate change could include changes in phenology of migratory pollinators and in the routes or destinations for their migration, changes in the phenology and distribution of non-migratory species, and changes in the host plants they visit for nectar and pollen. Plants face similar challenges with regard to changes in their distributions, their reproductive phenology, and interactions with both co-flowering species and pollinators (competition, facilitation, etc.). Unless pollinators and their host plants are...
Tipo: Presentation Palavras-chave: Ecology; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3583/version/1
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Ecological, phenological and biochemical significance of bimodal fruiting of Boletus alutaceus morgan Nature Precedings
Nandkumar Kamat.
Boletus alutaceus Morgan an ectomycorrhizal species was extensively studied from an ecologically fragmented microhabitt colonized by its' host plant Ficus benghalensis L. in the Goa University campus, Taleigao, Goa, India. Qualitatively the fruiting cycles were monitored for three years and thereafter detail ecological and morphological investigations were carried out in May and November 2004. A bimodal temporal fruting pattern emerged which may have significant ecological, phenological and biochemical role in EM dynamics.
Tipo: Presentation Palavras-chave: Biotechnology; Developmental Biology; Ecology; Genetics & Genomics; Microbiology; Plant Biology; Evolutionary Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/2835/version/1
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Some statistical aspects of the monitoring of GM plants Nature Precedings
Joe N. Perry.
There are several challenges in the monitoring of: (1) long-term effects of GM plants on the environment, and (2) effects that may be small in terms population change but significant in terms of the conservation of biodiversity. The difficulties are compounded by variability in environmental conditions between sites and years. Some examples will be given from the Farm Scale Evaluations of GMHT crops in the UK. However, the talk will focus on more recent developments, including:
(1)	LandsFACTS software developed during the SIGMEA project for modeling rotations that might be used in risk management 
(see...
Tipo: Presentation Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4463/version/1
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In silico comparative analysis of conserved genes expressed in solanaceous plants under abiotic stresses Nature Precedings
sanchita gupta; sunita singh dhawan; ashok sharma.
The gene expression data was studied in order to analyze genes involved in different abiotic stresses in solanaceous plants. Salt, temperature (heat & cold) and drought are the main abiotic stresses to the plants. Abiotic stress responses are important for sessile organisms like plants because they have to cope with environmental changes to survive. The idea was to analyze those differentially expressed genes, which show common expression in response to different abiotic stress conditions in each plant. Mitogen activated protein kinases (MAPK) are important mediators in signal transmission, connecting the perception of external stimuli to cellular responses. In plants, MAPKs plays a major role in the signaling of abiotic stresses. The expression...
Tipo: Poster Palavras-chave: Bioinformatics; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6717/version/1
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Detection of Sugarcane Yellow Leaf Luteovirus of India Nature Precedings
Rajarshi K. Gaur; G.P Rao; Axel Lehrer.
A luteovirus was found associated with midrib yellowing symptoms of sugarcane in India. The virus was earlier identified as sugarcane yellow leaf virus (SCYLV) on the basis of particle morphology and serological relationships. In this study, we attempted molecular characterization of an Indian isolate of SCYLV through reverse trascriptase-polymerase chain reaction (RT-PCR). Comparison showed that the deduced amino acid sequence share 98% homology with an isolate from Texas (cultivar CC85-964) and 100% homology with corresponding sequences of an Australian isolate of SCYLV (found in cultivar CP 65-357). Phylogenetic analysis also suggests that the coat protein of the SCYLV genome possesses different taxonomic affinities with other members of the family...
Tipo: Manuscript Palavras-chave: Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3388/version/1
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Data Mining of the Coffee Rust Genome Nature Precedings
David Octavio Botero-Rozo; William Giraldo; Alvaro Gaitan; Marco Cristancho; Diego M. Riaño-Pachon; Silvia Restrepo.
The genomes of nine isolates of _Hemileia vastatrix_, the causal agent of coffee leaf rust were sequenced by Illumina and 454. Quality control, cleaning and _de novo_ assemblies of data were performed. Since isolates were obtained from the field and it is not possible to produce axenic cultures of _H. vastatrix_, MEGAN software was used to evaluate contamination levels and to select contigs with fungal similarities. Mitochondrial contigs were identified and annotated by comparing this assembly against the _Puccinia_ genome. Furthermore, two transcriptomes from isolates of _H. vastatrix_ were assembled to complement the genomic data.
Tipo: Poster Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7034/version/1
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