SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Cozma,S.; Dima-Cozma,L.C.; Ghiciuc,C.M.; Pasquali,V.; Saponaro,A.; Patacchioli,F.R..

Currently, the potential for cardiovascular (CV) stress-induced risk is primarily based on the theoretical (obvious) side effects of stress on the CV system. Salivary cortisol and α-amylase, produced respectively by the hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic-adrenomedullary (SAM) system during stress response, are still not included in the routine evaluation of CV risk and require additional and definitive validation. Therefore, this article overviews studies published between 2010 and 2015, in which salivary cortisol and α-amylase were measured as stress biomarkers to examine their associations with CV/CMR (cardiometabolic risk) clinical and subclinical indicators. A comprehensive search of PubMed, Web of Science and Scopus...

Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats

Palavras-chave: Salivary stress biomarkers; Cardiometabolic risk (factors); Stress; Hypothalamic-pituitary-adrenal axis; Sympathetic adrenomedullary system.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200201

Aguiar,C.E.; Cadore,L.P.; Padoin,M.J.; Barbosa-Coutinho,L.M.; Lucion,A.B..

Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-day-old male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary...

Changes in the behavioral and immunological parameters of the mollusk Biomphalaria tenagophila induced by disruption of the circadian cycle as a consequence of continuous illumination

Palavras-chave: ACTH; Immunohistochemistry; Pituitary; Neonatal handling; Stress.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200013

Waissel,I.; Mineo,J.R.; Natal,C.L..

In the present investigation we studied some behavioral and immunological parameters of adult gastropod mollusk, Biomphalaria tenagophila, which have been reproducing for several generations under laboratory conditions. One group of gastropods was kept on a 14-h light/10-h dark cycle, corresponding to a regular circadian cycle, and another group was exposed to continuous light for 48 h. Animals were studied along (behavioral groups) or immediately after (immunological groups) 48 h of regular circadian cycle or continuous light conditions. Stopping/floating, dragging and sliding were the behavioral aspects considered (N = 20 for regular cycle; N = 20 for continuous illumination) and number of hemocytes/µl hemolymph was the immunological parameter studied (N...

Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus

Palavras-chave: Circadian cycle; Mollusk; Neuroimmunology; Stress; Behavior.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200014

Ruiz,F.S.; Andersen,M.L.; Zager,A.; Martins,R.C.S.; Tufik,S..

The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P < 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P < 0.001 and 4.4 ± 0.3 vs 0.9...

Estrous cycle and stress: influence of progesterone on the female brain

Palavras-chave: Sleep deprivation; Stress; Lymphopenia; Diabetes; Non-obese diabetic mouse; Swiss mice.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005

Lovick,T.A..

The female brain operates in a constantly changing chemical milieu caused by cyclical changes in gonadal hormones during the estrous cycle (menstrual cycle in women). Such hormones are highly lipophilic and pass readily from the plasma to the brain where they can influence neuronal function. It is becoming clear that the rapid reduction in peripheral circulating progesterone, which occurs during the late diestrous phase of the cycle, can trigger a withdrawal-like response, in which changes in GABA A receptor expression render hyper-responsive certain brain areas involved in processing responses to stressful stimuli. The periaqueductal gray matter (PAG) is recognised as an important region for integrating anxiety/defence responses. Withdrawal from...

Effects on prolactin secretion and binding to dopaminergic receptors in sleep-deprived lupus-prone mice

Palavras-chave: Female; Estrous cycle; Stress; Anxiety; Progesterone; Neurosteroid replacement.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400005

Palma,B.D.; Hipolide,D.C.; Tufik,S..

Sleep disturbances have far-reaching effects on the neuroendocrine and immune systems and may be linked to disease manifestation. Sleep deprivation can accelerate the onset of lupus in NZB/NZWF1 mice, an animal model of severe systemic lupus erythematosus. High prolactin (PRL) concentrations are involved in the pathogenesis of systemic lupus erythematosus in human beings, as well as in NZB/NZWF1 mice. We hypothesized that PRL could be involved in the earlier onset of the disease in sleep-deprived NZB/NZWF1 mice. We also investigated its binding to dopaminergic receptors, since PRL secretion is mainly controlled by dopamine. Female NZB/NZWF1 mice aged 9 weeks were deprived of sleep using the multiple platform method. Blood samples were taken for the...

Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress

Palavras-chave: Systemic lupus erythematosus; NZB/NZWF1 mice; Prolactin; Stress; Autoradiography; Dopamine transporter.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300012

Torres,R.L.; Torres,I.L.S.; Gamaro,G.D.; Fontella,F.U.; Silveira,P.P.; Moreira,J.S.R.; Lacerda,M.; Amoretti,J.R.; Rech,D.; Dalmaz,C.; Belló,A.A..

Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g...

Restraint-induced hypoactivity in an elevated plus-maze

Palavras-chave: Stress; TBARS; TRAP; Free radicals; Lungs; Oxidative stress.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004

Padovan,C.M.; Guimarães,F.S..

Rodents submitted to restraint stress show decreased activity in an elevated plus-maze (EPM) 24 h later. The objective of the present study was to determine if a certain amount of time is needed after stress for the development of these changes. We also wanted to verify if behavioral tolerance of repeated daily restraint would be detectable in this model. Male Wistar rats were restrained for 2 h and tested in the EPM 1, 2, 24 or 48 h later. Another group of animals was immobilized daily for 2 h for 7 days, being tested in the EPM 24 h after the last restraint period. Restraint induced a significant decrease in the percent of entries and time spent in the open arms, as well as a decrease in the number of enclosed arm entries. The significant effect in the...

Prolactin and cortisol levels in women with endometriosis

Palavras-chave: Stress; Restraint; Elevated plus-maze; Behavioral changes; Tolerance.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100011

Lima,A.P.; Moura,M.D.; Rosa e Silva,A.A.M..

Endometriosis is a progressive estrogen-dependent disease affecting women during their reproductive years. The objective of the present study was to investigate whether endometriosis is associated with stress parameters. We determined cortisol and prolactin levels in serum, peritoneal and follicular fluid from infertile women with endometriosis and fertile women without the disease. The extent of the disease was staged according to the revised American Fertility Society classification (1997). Serum and peritoneal fluid were collected from 49 women aged 19 to 39 years undergoing laparoscopy. Eighteen women had stage I-II endometriosis and 10 had stage III-IV. Controls were 21 women undergoing laparoscopy for tubal sterilization. Follicular fluid was...

Effect of repeated stress on novelty-induced antinociception in rats

Palavras-chave: Endometriosis; Stress; Infertility; Prolactin; Cortisol.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800015

Torres,I.L.S.; Vasconcellos,A.P.; Silveira Cucco,S.N.; Dalmaz,C..

There is extensive evidence that acute stress induces an analgesic response in rats. On the other hand, repeatedly stressed animals may present the opposite effect, i.e., hyperalgesia. Furthermore, exposure to novelty is known to induce antinociception. The effects of repeated restraint stress on nociception after exposure to novelty, as measured by the tail-flick latency (TFL), were studied in adult male rats. The animals were stressed by restraint 1 h daily, 5 days a week for 40 days. The control group was not submitted to restraint. Nociception was assessed with a tail-flick apparatus. After being familiarized with the TFL apparatus, each group was subdivided into two other groups, i.e., with or without novelty. Animals were subjected to the TFL...

Evidence of hemispheric specialization in marmosets (Callithrix penicillata) using tympanic membrane thermometry

Palavras-chave: Pain; Stress; Repeated stress; Nociception; Novelty.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200012

Tomaz,C.; Verburg,M.S.; Boere,V.; Pianta,T.F.; Belo,M..

Recent studies have employed tympanic thermometry to assess lateralization of cognitive and emotional functions in primates. However, no studies using this technique have investigated the possibility of hemispheric specialization in New World monkeys. Therefore, the aim of the present study was to investigate tympanic membrane (TM) temperature asymmetries and their possible correlation with stress responses in marmosets (Callithrix penicillata). Infrared TM thermometry was completed bilaterally in 24 animals (14 males and 10 females) during a stressful situation of capture and restraint. There were no significant differences between gender. A significant negative correlation was observed between TM temperature of the right ear and the number of captures (r...

Effects of acupuncture on behavioral, cardiovascular and hormonal responses in restraint-stressed Wistar rats

Palavras-chave: Laterality; Tympanic temperature; Stress; New World monkey.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700012

Guimarães,C.M.; Pinge,M.C.M.; Yamamura,Y.; Mello,L.E.A.M..

Stress is a well-known entity and may be defined as a threat to the homeostasis of a being. In the present study, we evaluated the effects of acupuncture on the physiological responses induced by restraint stress. Acupuncture is an ancient therapeutic technique which is used in the treatment and prevention of diseases. Its proposed mechanisms of action are based on the principle of homeostasis. Adult male Wistar EPM-1 rats were divided into four groups: group I (N = 12), unrestrained rats with cannulas previously implanted into their femoral arteries for blood pressure and heart rate measurements; group II (N = 12), rats that were also cannulated and were submitted to 60-min immobilization; group III (N = 12), same as group II but with acupuncture needles...

Environmental blue light prevents stress in the fish Nile tilapia

Palavras-chave: Stress; Acupuncture; Catecholamines; Corticosterone.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200011

Volpato,G.L.; Barreto,R.E..

The present study aimed to test the effects of blue, green or white light on the stress response of the Nile tilapia, Oreochromis niloticus (L.). Each color was tested on two groups of isolated adult Nile tilapia (8 replicates each): one being subjected to confinement stress, and the other not (control). A different environmental color was imposed on each compartment by covering the light source with cellophane of the respective color (green or blue; no cellophane was used for white light). The intensity of green, white and blue lights was 250, 590 and 250 lux, respectively. Basal plasma cortisol levels were determined for each fish prior to the experimental procedures. The fish were confined by being displaced toward one side of the aquarium using an...

Concomitant stress potentiates the preference for, and consumption of, ethanol induced by chronic pre-exposure to ethanol

Palavras-chave: Stress; Animal welfare; Light color; Cortisol; Nile tilapia; Oreochromis niloticus (L.).

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800011

Morais-Silva,G.; Fernandes-Santos,J.; Moreira-Silva,D.; Marin,M.T..

Ethanol abuse is linked to several acute and chronic injuries that can lead to health problems. Ethanol addiction is one of the most severe diseases linked to the abuse of this drug. Symptoms of ethanol addiction include compulsive substance intake and withdrawal syndrome. Stress exposure has an important role in addictive behavior for many drugs of abuse (including ethanol), but the consequences of stress and ethanol in the organism when these factors are concomitant results in a complex interaction. We investigated the effects of concomitant, chronic administration of ethanol and stress exposure on the withdrawal and consumption of, as well as the preference for, ethanol in mice. Male Swiss mice (30–35 g, 8-10 per group) were exposed to an ethanol liquid...

Stress responses of the fish Nile tilapia subjected to electroshock and social stressors

Palavras-chave: Ethanol; Addiction; Stress; Free-bottle choice; Withdrawal.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100606

Barreto,R.E.; Volpato,G.L..

Plasma cortisol and glucose levels were measured in 36 adult Nile tilapia males, Oreochromis niloticus (standard length, mean ± SD, 14.38 ± 1.31 cm), subjected to electroshock and social stressors. Pre-stressor levels were determined 5 days after the adjustment of the fish to the experimental aquaria (1 fish/aquarium). Five days later, the effects of stressors on both cortisol and glucose levels were assessed. The following stressors were imposed for 60 min: pairing with a larger resident animal (social stressor), or a gentle electroshock (AC, 20 V, 15 mA, 100 Hz for 1 min every 4 min). Each stressor was tested in two independent groups, one in which stress was quantified immediately after the end of the 60-min stressor imposition (T60) and the other in...

Isolation and characterization of Caulobacter mutants impaired in adaptation to stationary phase

Palavras-chave: Stress; Aggression; Agonistic behavior; Fighting; Social stress; Electroshock.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200012

BJM

Italiani,Valéria C. S.; Marques,Marilis V..

The entry into stationary phase causes a change in the pattern of gene expression of bacteria, when the cells must express a whole set of genes involved mainly with resistance to starvation and to environmental stresses. As an attempt to identify genes important for the survival of Caulobacter crescentus in stationary phase, we have screened a library of 5,000 clones generated by random transposon mutagenesis for mutants that showed reduced viability after prolonged growth. Four clones were selected, which displayed either lower viability or a longer time of recovery from stationary phase. The genes disrupted were identified, and the gene products were found to be mainly involved with amino acid metabolism (glutamate N-acetyltransferase,...

Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays

Palavras-chave: Stationary phase; Stress; Transposon; Bacteria.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100018

BJM

Martins,Flaviano S.; Miranda,Ieso C.; Rosa,Carlos A.; Nicoli,Jacques R.; Neves,Maria J..

Probiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of the host when ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed by in vitro assays simulating gastrointestinal environment to determine the ability to survive in the digestive tract. In the present study, the possibility of extrapolation of data obtained in in vitro assays to in vivo conditions was studied using five Saccharomyces cerevisiae strains isolated from Brazilian Atlantic rain forest. Trehalose contents and survival after exposure to a combination of physiological stresses generally found in the gastrointestinal tract of humans were determined for the five yeasts and compared to the behavior...

Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and pathogenesis genes in response to 6°c, 65°c and ph 2.0

Palavras-chave: Probiotic; Saccharomyces boulardii; Saccharomyces cerevisiae; Stress; Trehalose.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100012

BJM

Pribylova,Radka; Kralik,Petr; Donnelly,Neysan; Matiasovic,Jan; Pavlik,Ivo.

The aim of this work was to study the expression of selected Mycobacterium avium subsp. paratuberculosis (MAP) genes connected with MAP virulence, adhesion and stress response. The temperature of 6°C and 65°C were chosen with regard to the food industry, storage conditions (refrigerator) and low-temperature pasteurization. A pH of 2.0, using lactic acid, was selected to mimic the natural environment of the stomach. Expression of selected genes was studied using real time reverse transcription PCR on three different MAP isolates. MAP isolates were chosen according to the number of their preceding cultivations. While isolates 8672 and 8819 were previously cultivated only once, MAP isolate 12146 went through four passages. Different expression profiles were...

Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant

Palavras-chave: MAP; Johne's disease; Stress; Treatment; Real time PCR.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200049

Braz. J. Plant Physiol.

Costa,Cibele T.; Strieder,Mércio L.; Abel,Stephen; Delatorre,Carla A..

Changes in root architecture are an important adaptive strategy used by plants in response to limited nutrient availability to increase the odds of acquiring them. The quiescent center (QC) plays an important role by altering the meristem activity causing differentiation and therefore, inducing a determinate growth program. The arabidopsis mutant pdr23 presents primary short root in the presence of nitrate and is inefficient in the use of nucleic acids as a source of phosphorus. In this study the effect of the pdr23 mutation on the QC maintenance under low phosphorus (P) and/or nitrogen is evaluated. QC identity is maintained in wild-type in the absence of nitrate and/or phosphate if nucleic acids can be used as an alternative source of these nutrients,...

Physiological response of oil palm interspecific hybrids (Elaeis oleifera H.B.K. Cortes versus Elaeis guineensis Jacq.) to water deficit

Palavras-chave: QC25::iudA; Nutrient deficiency; Root development; Determinate root growth program; Stress.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300006

Braz. J. Plant Physiol.

Méndez,Yurany Dayanna Rivera; Chacón,Leonardo Moreno; Bayona,Cristihian Jarry; Romero,Hernán Mauricio.

Water supply is the main yield-limiting abiotic factor of oil palm in Colombia. To determine the effect of water deficit on the physiology of oil palm and to assess the tolerance degree to this condition, interspecific Elaeis oleifera versus Elaeis guineensis 'U1273', 'U1859', 'U1914', and 'U1937' hybrids were planted for 60 days in soil with different water potentials (-0.042, -0.5, -1.0, and -2.0 MPa) in a complete randomized factorial design with three replications. The water potential, osmotic adjustment molecules (total and reducing sugar content), gas exchange (photosynthesis rate, stomatal conductance, water use efficiency, and leaf respiration), and vegetative growth (height, bulb diameter, leaf area, number of leaves, total dry matter, and...