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| Registros recuperados: 13.863 | |
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| Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M. |
| The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant.... |
| Tipo: Journal article |
Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010 |
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| Brandelli,Adriano; Riffel,Alessandro. |
| The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Enzyme; Keratin; Protease. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007 |
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| Tsegay,Berhanu A.; Lund,Leidulf; Nilsen,Jarle; Olsen,Jorunn E.; Molmann,Jorgen M.; Ernsten,Arild; Juntttila,Olavi. |
| The effect of Red light (R), Far-red light (FR) and R/FR combinations on shoot growth of latitudinal ecotypes of B. pendula was studied using special diodes that emit monochromatic lights. When a 12 hrs PAR (110 µmol m-2 s-1) was extended with R, FR or R/FR ratios, lower intensities of monochromatic lights could not prevent growth cessation. At 25 µmol m-2 s-1, FR compared to R enhanced stem elongation in all ecotypes. This was due to the inhibitive effect of R on internode elongation. When day-length was extended by R/FR at various ratios, there was continuous shoot elongation, but was found to be declining with increasing ratios. The more the R, the shorter were the internodes of each plant. B. pendula ecotypes produced branches when... |
| Tipo: Journal article |
Palavras-chave: Diode; Ecotype; Monochromatic; Photomorphogenesis; Phytochrome; Photosynthetically active radiation; PAR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100005 |
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| Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Kumar,Vinod; Lakshmanan,Venkatachalam; Venkataramareddy,Sreedhar Reddampalli; Aswathanarayana,Ravishankar Gokare. |
| The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium rhizogenes; Auxins; Elicitation; T-DNA; Thermostability; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008 |
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| Montealegre,Jaime R; Herrera,Rodrigo; Velásquez,Juan Carlos; Silva,Polyana; Besoaín,Ximena; Pérez,Luz María. |
| Trichoderma harzianum 650 (Th650) and Paenebacillus lentimorbus 629 (Pl629) selected earlier for their ability to control Rhizoctonia solani, Fusarium solani and F. oxysporum in vitro, were applied alone or combined with solarization (summer assay) and/or with methyl bromide (MeBr) (summer and winter assays) to a soil with a high inoculum level, for the control of tomato root rot caused by the complex F. oxysporum f. sp. lycopersici - Pyrenochaeta lycopersici - Rhizoctonia solani. Evaluations were also performed independently for root damage caused by P. lycopersici, and also for R. solani in the summer assay. MeBr decreased tomato root damage caused by the complex from 88.7% to 21.2% and from 78.4% to 35.7% in the summer and in the winter assay,... |
| Tipo: Journal article |
Palavras-chave: Fusarium oxysporum; Lycopersicon esculentum; Pyrenochaeta lycopersici; Rhizoctonia solani; Solarization. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300004 |
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| Li,Biaoru. |
| Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is... |
| Tipo: Journal article |
Palavras-chave: Genome; Genomic expression; Genomic expression analysis at single cell; Single cell. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100011 |
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| Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando. |
| The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,... |
| Tipo: Journal article |
Palavras-chave: Cloning; Genome; Social representations; Transgenic. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003 |
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| Vásquez,Manuel A. N.; Folegatti,Marcos V.; Dias,Nildo da S.; Silva,Cláudio R. da. |
| O objetivo deste trabalho foi estudar o efeito do ambiente protegido cultivado com melão (Cucumis melo L.) sobre os elementos meteorológicos e sua relação com as condições externas. O experimento foi conduzido no período de 5-10-2001 a 7-1-2002, em estufa plástica de 420 m², construída com estrutura metálica galvanizada, com dois vãos de altura central de 4,6 m e lateral de 3,0 m, composto de quatro janelas frontais, cobertas com filme de polietileno transparente de alta densidade, com aditivo ultravioleta e espessura de 150 µm. A irrigação foi efetuada por gotejamento, com lâmina total de 279,6 mm, manejada utilizando um minitanque instalado no interior do ambiente. Os elementos meteorológicos foram obtidos por sistema de aquisição automático instalado no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Radiação; Evapotranspiração; Irrigação; Cucumis melo L.. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162005000100015 |
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| Silva,Fabio M. da; Lucas Junior,Jorge de; Benincasa,Mario; Oliveira,Ezequiel de. |
| Estudos foram desenvolvidos para dimensionar e adaptar o injetor do queimador principal de um aquecedor de água tipo acumulação de 75 L. O diâmetro do injetor foi redimensionado em função da pressão de serviço de 100 mm H2O e poder calorífico inferior do biogás de 21.600 kJ m-3 n, garantindo a manutenção da potência calorífica do equipamento de 20.900 kJ h-1. Os resultados demonstraram que o queimador adaptado operou com biogás adequadamente, com chama estável. A eficiência média do aquecedor foi de 68%, para ganho térmico de 36,7 ºC, correspondendo à temperatura final da água igual a 62,7 ºC, sendo consumido 0,796 m³n de biogás, aquecendo 75 L de água em 72 minutos. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biogás; Poder calorífico; Digestão anaeróbia. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162005000300005 |
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| Marcussi,Francisco F. N.. |
| Este estudo teve o objetivo de determinar os teores de macronutrientes (N, P, K, Ca, Mg e S) nos diferentes órgãos do híbrido de pimentão Elisa, sob fertirrigação e ambiente protegido, por meio de coletas de plantas durante o ciclo. Ao final do experimento, obtiveram-se os teores dos macronutrientes no fruto, folha, caule, raiz e na planta como um todo, em cada etapa de crescimento. A fertilização no ciclo estudado, 140 dias, foi feita via irrigação localizada (gotejamento). O experimento foi desenvolvido nas dependências do Departamento de Recursos Naturais - Ciência do Solo, FCA-UNESP, Câmpus de Botucatu - SP, e constou de oito tratamentos (épocas de coleta de planta: 0; 20; 40; 60; 80; 100; 120 e 140 dias após o transplante das mudas) e quatro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gotejamento; Concentrações de macronutrientes; Capsicum annuum L. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162005000300009 |
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| Registros recuperados: 13.863 | |
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