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| Registros recuperados: 23 | |
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| Labreuche, Yannick; Pallandre, Laurane; Ansquer, Dominique; Herlin, Jose; Wapotro, Billy; Le Roux, Frederique. |
| Two recurring syndromes threaten the viability of the shrimp industry in New Caledonia, which represents the second largest export business. The "Syndrome 93" is a cold season disease due to Vibrio penaeicida affecting all shrimp farms, while the "Summer Syndrome" is a geographically restricted vibriosis caused by a virulent lineage of Vibrio nigripulchritudo. Microbiological procedures for diagnosis of these diseases are time-consuming and do not have the ability to discriminate the range of virulence potentials of V. nigripulchritudo. In this study, we developed a multiplex PCR method to simultaneously detect these two bacterial species and allow for pathotype discrimination. The detection limits of this assay, that includes an internal amplification... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00063/17398/15169.pdf |
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| Beliaeff, Benoit; Laugier, Thierry; Chim, Liet; Coatanea, Denis; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Lemaire, Pierrette; Lemonnier, Hugues; Morvan, Patrice; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Soulard, Benoit; Wabete, Nelly; Walling, Emilie; Ansquer, Dominique; Castex, Mathieu; Lucas, Ronan; Reynaud, Yann; Ifremer, LEAD-NC,. |
| Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces,... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00199/31006/35663.pdf |
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| Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques. |
| Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two... |
| Tipo: Text |
Palavras-chave: Vibriosis; Survival; Growth; Penaeus Litopenaeus stylirostris; Shrimp; Cross breeding; Genetic improvement. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4316.pdf |
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| Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene. |
| Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical... |
| Tipo: Text |
Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.. |
| Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the... |
| Tipo: Text |
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf |
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| Aguirre-guzman, Gabriel; Labreuche, Yannick; Ansquer, Dominique; Espiau, Benoit; Levy, Peva; Ascencio, Felipe; Saulnier, Denis. |
| The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus stylirostris; Vibrio nigripulchritudo; Vibrio penaeicida. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2807.pdf |
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| Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly. |
| New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government... |
| Tipo: Text |
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf |
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| Charme, Marion; Ansquer, Dominique; Vourey, Elodie; Walling, Emilie; Beliaeff, Benoit; Labreuche, Yannick. |
| Experimental infections together with epidemiological studies have shown that pathogenic and non‐pathogenic isolates of V. nigripulchritudo co‐existed in shrimp farm environment (2, 3). Moreover, obtained results also demonstrated that the “summer syndrome”was caused by a single, possibly emerging, cluster of virulent strains. Consequently, it was hypothesized that pathogenic strains of V. nigripulchritudo may persist from one year to the next in the shrimp farm environment and re‐develop inside the grow‐out system at the following rearing cycle (3). This study was therefore aimed at determining whether V. nigripulchritudo isolates may survive, or not, in a shrimp pond bottom soil during a 18‐week drying period. To this end, V.nigripulchritudo mapping was... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00197/30874/29242.pdf |
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| Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick. |
| In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the... |
| Tipo: Text |
Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf |
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| Goyard, Emmanuel; Goarant, Cyrille; Bachere, Evelyne; De Lorgeril, Julien; Mugnier, Chantal; Ansquer, Dominique; Broutoi, Francis; Brun, Pierre; Imbert, Frederic; Justou, Carole; Maillez, Jean-rene; Patrois, Jacques; Pham, Dominique; Peignon, Jean-marie. |
| The New-Caledonian shrimp industry is based on the controlled reproduction of the shrimp Litopenaeus stylirostris, a species which was introduced in the 80s. The major difficulty to which the industry has been faced for 10 years is the occurrence of the "syndrome 93", which corresponds to mortality phases when the temperature falls down in April-May-June. This mortality is associated to the pathogenic bacteria Vibrio penaecida and is expressed at different levels which are variable from year ta year and from pond to pond. No resistance to this pathology has been developed spontaneously. This is likely due to the protocole used to rear spawners, which does not allow to implement an efficient selective pressure at each generation An experimental selection... |
| Tipo: Text |
Palavras-chave: Amélioration génétique; P. styliroslris; Résistance; Syndrome 93; Lyzozyme; Genetic improvement; P. styliroslris; Résistance; Syndrome 93; Lyzozyme. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00109/22020/19643.pdf |
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| Goyard, Emmanuel; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Goarant, Cyril; Patrois, Jacques; Peignon, Jean; Pham, Dominique. |
| Selective breeding of shrimp for viral resistance is established for species like L. vannamei and L. stylirostris. However, selection for bacterial infection resistance is not documented. In New Caledonia, "Syndrome 93" is a vibriosis that affects cultured shrimps during the cool season with survival from 15 to 30%. Current practises for breeders rearing at low densities do not improve the resistance of the offsprings mainly because selection pressures for "Syndrome 93" are low. Even after the fifth generation, survival improvement is not significant. |
| Tipo: Text |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00117/22848/20658.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique. |
| A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp... |
| Tipo: Text |
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf |
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| Goudenege, David; Labreuche, Yannick; Krin, Evelyne; Ansquer, Dominique; Mangenot, Sophie; Calteau, Alexandra; Medigue, Claudine; Mazel, Didier; Polz, Martin F.; Le Roux, Frederique. |
| Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively... |
| Tipo: Text |
Palavras-chave: Horizontal gene transfer; Nigritoxin; Phylogeny; Shrimp; Virulence; Vibrio. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27117/28369.pdf |
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| Pham, Dominique; Vourey, Elodie; Ansquer, Dominique; Walling, Emilie. |
| Seawater, algae, micro-diets and Artemia are the main inputs in shrimp larval rearing. Each of them are potential carriers of pathogenic bacteria in the rearing media and weaken the animal in its early life stages. Daily monitoring of rearing water and larvae showed a bacterial spike after the first live feed meal. Vibrio settlement can be enhancedby giving Artemia with their incubation water where vibrio concentration can reach up to 105 CFU per milliliter. This study showed that simple measures such as rinsing and supplying Artemia with freshwater allowed a significant drop in their bacterial load. |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00118/22971/20801.pdf |
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| Patrois, Jacques; Goyard, Emmanuel; Peignon, Jean-marie; Dufour, Robert; Ansquer, Dominique. |
| A new strain of L. stylirostris was introduced in New Caledonia. Quarantine facilities were set up inland using RAS and artificial seawater. After 5 months of rearing, all the 16 initial families were still represented with a 50% average survival. Three samplings for known pathogens were made during that period, all of them negative. Half the animals were taken to outdoor ponds for rearing to reproduction size when the rest was used to test different arrangements for a biosecure rearing until reproduction. Numerous spawnings and nauplii were obtained but larval rearings could not be completed. Different options are considered for the set up of a biosecure facility allowing the rearing and the breeding of pathogen free strains of shrimp. |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00117/22849/20659.pdf |
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| Nicolas, Jean-louis; Ansquer, Dominique; Cochard, Jean-claude. |
| A bacterial strain belonging to the genus Vibrio was involved in a recurrent outbreak of disease in Manila clam Tapes philippinarum larvae at a commercial hatchery, This vibrio, named VTP for convenience, was not related to the known pathogenic vibrios and possessed unusual characteristics, Only Manila clam larvae and postlarvae were affected by the disease, Oyster and scallop larvae were not affected by vrp, Survival of vrp was found to be of short duration in seawater but even a very small number of cells (1 cell per 5 ml) could initiate the disease, VTP did not grow on thiosulfate-citratebile-sucrose agar and was resistant to chloramphenicol. An epidemiological study utilizing bioassays revealed the presence of VTP in adult bivalves and in the... |
| Tipo: Text |
Palavras-chave: Bacterial diseases; Clam culture; Larvae; Pathogenic bacteria; Tapes philippinarum; Vibrio. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00037/14872/12198.pdf |
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| Registros recuperados: 23 | |
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