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	Provedor de dados Nature Precedings (2) Autor Jawed Iqbal (2) Najmul Islam (2) Abbas Ali Mahdi (1) Ajay Kumar (1) Farzana Bano (1) Hamida Thakur (1) Irfan Ahmad Ansari (1) Nazarul Hasan (1) Nazia Hasan (1) Tamkin Khan (1) Zeeshan Fatima (1) Mais... Palavra-chave Microbiology (2) Molecular Cell Biology (2) Immunology (1) Tipo do documento Manuscript (1) Poster (1) Ano 2008 (2) País United Kingdom (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte Nature Precedings Jawed Iqbal; Ajay Kumar; Najmul Islam. The H37Rv strain of MTB was grown in modified Sauton’s medium till mid-log phase. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient sedimentation on Ficoll-Paque separation medium. Adherent monocytes obtained from PBMC’s were infected with H37Rv for 90 min. in the ratio of 1:1 (1 bug: 1 cell). Thereafter, after washing, infected cells were co-cultured with varying doses of neem extract for 24 hrs, and harvested subsequently. Modulation of secreted TNF-[alpha], iNOS and MTB Ag85 complex expressions in culture supernatants was estimated by ELISA.
We report the high basal expression of secreted tumor necrosis factor-[alpha] (TNF-[alpha]), inducible nitric oxide synthase (iNOS) and MTB Ag85... Tipo: Poster Palavras-chave: Microbiology; Molecular Cell Biology. Ano: 2008 URL: http://precedings.nature.com/documents/1562/version/1 Allicin from garlic suppresses TNF-α and augments IFN-γ expressions in monocyte cultures from patients with Vaginitis Nature Precedings Najmul Islam; Jawed Iqbal; Nazarul Hasan; Zeeshan Fatima; Hamida Thakur; Nazia Hasan; Abbas Ali Mahdi; Tamkin Khan; Farzana Bano; Irfan Ahmad Ansari. Objective: We tried to explore beneficial effects of allicin- a natural antioxidant from garlic in the possible management of vaginitis. 
Methods: Peripheral blood mononuclear cells (PBMC’s) were isolated from blood of patients having vaginal infections as well as normal healthy subjects as per our published protocol. Monocytes (MN’s) from above PBMC’s were adhered, rested overnight and cultured without or with varying doses of allicin (0-500 ng/ml). Some cultures received SN50 and SN50M (100 ug/ml). After 24 hrs cultures, the cells were harvested and the supernatants subjected to secreted TNF-α and sIFN-γ assays by ELISA. Treated/untreated harvested cells were subjected to glutathione... Tipo: Manuscript Palavras-chave: Immunology; Microbiology; Molecular Cell Biology. Ano: 2008 URL: http://precedings.nature.com/documents/1560/version/1 Registros recuperados: 2 Primeira ... 1 ... Última

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