|
|
|
| Registros recuperados: 32 | |
|
|
| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
| |
|
|
| Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia. |
| Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other... |
| Tipo: Text |
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf |
| |
|
|
| Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Goulletquer, Philippe; Ratiskol, Jacqueline; Garnier, Jacqueline. |
| An hydrobiological monitoring survey has been carried out in the Bay of Marennes-Oléron since 1977. Five stations, distributed over the Bay, were sam pied twice a month. Spatial and temporal trends of the physical characteristics (i.e., seawater, temperature, salinity, dissolved oxygen and suspended matter), nutrients (azote, nitrite, nitrate, silicate) and organic variables (chlorophyll a and pheopigments) were assessed by using a statistical time-series analysis mode\. It was used to distinguish seasonal variability from long term trends. Pluviometric data as weil as the Charente River fluxes were similary analyzed by the model. The seasonal variability showed an homogeneous pattern over the five stations for all parameters, but the site-dependent... |
| Tipo: Text |
Palavras-chave: Shellfish farming; Overall trends; Seawater seasonal variability; Marennes Oleron Bay; Hydrology; Conchyliculture; Tendances; Variations saisonnières; Marennes Oléron; Hydrologie. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-6379.pdf |
| |
|
| |
|
|
| Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia. |
| Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf |
| |
|
|
| Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire. |
| Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf |
| |
|
| |
|
|
| Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800... |
| Tipo: Text |
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf |
| |
|
| |
|
|
| Faury, Nicole; Ratiskol, Jacqueline. |
| Les conditions météorologiques semblaient cette année à priori favorables à la reproduction des moules. Cependant, le maturation des gonades a été très lente. Ainsi, les premières pontes, de faible importance, ont eu lieu début Avril. Plusieurs émissions tardives sont apparues fin Mai et se sont poursuivies jusqu'au début du mois de Juillet. Elles n'ont pas été importantes et ont conduit à une faible quantité de larves au stade fixation. Un captage "moyen" a été observé début Mai. Ensuite, la présence de quelques larves grosses n'a pas permis un recrutement suffisant. Généralement, suite à la période de reproduction les moules reconstituent rapidement leur réserve; or cette année, elles sont restées maigres jusqu'à la fin Juillet. Le fort déficit... |
| Tipo: Text |
Palavras-chave: Reproduction; Captage; Naissain; Crassostrea gigas; Marennes Oléron; Mytilus edulis. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00072/18285/15857.pdf |
| |
|
|
| Roger, Olivier; Kervarec, Nelly; Ratiskol, Jacqueline; Colliec-jouault, Sylvia; Chevolot, Lionel. |
| The structure of the extracellular polysaccharide produced by the mesophilic species, Alteromonas infernus, found in deep-sea hydrothermal vents and grown under laboratory conditions, has been investigated using partial depolymerization, methylation analysis, mass spectrometry and NMR spectroscopy. The repeating units of this polysaccharide is a nonasaccharide with the following structure: [GRAPHICS] (C) 2004 Elsevier Ltd. All rights reserved. |
| Tipo: Text |
Palavras-chave: Hydrothermal vent; Structure; Bacterial polysaccharide; Alteromonas. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2112.pdf |
| |
|
|
| Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
| Les agriculteurs aménageant quelques centaines d'hectares du marais doux de Hiers Brouage (mise à plat, drainage) afin de le mettre en culture, il était important de pouvoir mesurer l'impact que peuvent avoir les rejets de drainage-pompage dans le milieu marin par l'intermédiaire des chenaux ostréicoles. Ceci afin d'éviter d'éventuels conflits entre groupes socio-professionnels (agriculteurs et conchyliculteurs) en proposant des mesures et de solutions. Un suivi est donc effectué à la demande des administrations, en collaboration avec l'INRA et le CEMAGREF, lesquels assurent le suivi de la lagune de décantation des eaux de drainage. L'IFREMER prenant en compte le milieu récepteur (chenaux du Grand Garçon et de Brouage). A raison de cinq sériées de... |
| Tipo: Text |
Palavras-chave: Qualité des eaux; Agriculture; Rejets; Marais maritimes; Chenal de Brouage; Charente-Maritime. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00087/19850/17500.pdf |
| |
|
|
| Faury, Nicole; Ratiskol, Jacqueline. |
| La décontamination bactériologique des huitres Crassostrea gigas a été étudiée expérimentalement dans des bassins dégorgeoirs de 13 m3 de conception proche de la configuration normale d'une station de purification française. Les huîtres contaminées artificiellement ont été placées dans des conditions dites "naturelles" d'épuration. Il n'a pas été utilisé de procédé physique ou chimique de purification de l'eau. Elles ont été soumises sur trois périodes de l'année à trois conditions différentes de renouvellement d'eau : quotidien, biquotidien ou apport continu, le témoin étant caractérisé par l'absence de tout renouvellement d'eau. Les résultats permettent de dégager un ensemble de remarques sur l'état physiologique, sur l'activité métabolique des... |
| Tipo: Text |
Palavras-chave: Décontamination; Coliformes fécaux; Streptocoques fécaux; Bassins; Purification. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00050/16156/13639.pdf |
| |
|
| |
|
|
| Faury, Nicole; Ratiskol, Jacqueline; Ratiskol, Gilles. |
| La surveillance bactériologique de 1989 à 1992 du bassin de Marennes-Oléron et de la rive droite de la Gironde met en évidence la bonne qualité du milieu. Les dépassements de la norme (>300 coliformes fécaux/100 g de chair et liquide intervalvaire) sont peu fréquents. Quelques pollutions sporadiques peuvent apparaître, le plus souvent en été, et sont liées au dysfonctionnement passager des systèmes d'assainissement (postes de relèvement, stations d'épuration) ou aux infiltrations d'eaux usées dans les réseaux pluviaux. L'application de la directive européenne de 1991 aurait une incidence limitée sur le classement des zones de production conchylicoles du bassin de Marennes-Oléron. |
| Tipo: Text |
Palavras-chave: Bactériologie; Surveillance; Coliformes fécaux; Marennes Oléron; Gironde. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00014/12497/9344.pdf |
| |
|
|
| Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
| L'étude entreprise en 1985 sur la qualité des eaux de rejet de la zone cultivée de l'Ilot des Tannes, dans le marais de Moeze, examine maintenant les résultats de la deuxième année de culture intensive. Rappelons que cette étude a pour but d'apprécier la qualité physico-chimique des eaux de ruissellement et le drainage arrivant dans le milieu ostréicole par l'intermédiaire du Chenal de Brouage. Les compagnes des deux années précédentes après un point zéro et une première année de mise en culture avaient fait apparaître, outre la présence d'insecticides rémanents, une dominance qualitative et quantitative des herbicides aussi bien dans l'eau que dans la matière vivante. Chaque année, des difficultés techniques différentes limitent l'information recueillie,... |
| Tipo: Text |
Palavras-chave: Qualité des eaux; Marais Maritime; Agriculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00087/19849/17499.pdf |
| |
|
|
| Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia. |
| The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the... |
| Tipo: Text |
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf |
| |
|
| |
|
|
| Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (> 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf |
| |
|
| |
| Registros recuperados: 32 | |
|
|
|
