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| Registros recuperados: 32 | |
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| Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine. |
| In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were... |
| Tipo: Text |
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf |
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| Cheret, Romuald; Delbarre Ladrat, Christine; De Lamballerie Anton, Marie; Verrez-bagnis, Veronique. |
| Post mortem tenderization is one of the most unfavourable quality changes in fish muscle and this contrasts with muscle of mammalian meats. The tenderization can be partly attributed to the acid lysosomal cathepsins and cytosolic neutral calcium-activated calpains. In this study, these proteases from fish and bovine muscles were quantified and compared. The cathepsin B and L activities were in more important amounts in sea bass white muscle than in bovine muscle. On the other hand, cathepsin D activity was 1.4 times higher in meat that in fish muscle, while cathepsin H was negligible in both muscles. Calpain activities were similar in both types of muscle. Moreover, calpastatin (calpain endogenous inhibitor) level is 3.9 times higher in sea bass white... |
| Tipo: Text |
Palavras-chave: Meat; Fish; Protease; Cathepsin; Calpain. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3646.pdf |
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| Verrez-bagnis, Veronique; Soree, Marion; Passerini, Delphine; Kolypczuk, Laetitia; Marchand, Laetitia; Bonnetot, Sandrine; Fecamp, Florian; Lozach, Solen; Hervio Heath, Dominique; Delbarre Ladrat, Christine. |
| Vibrio spp. are ubiquitous marine bacteria that are ecologically and metabolically diverse members of planktonic and animal associated microbial communities. They encompass the ancient and well-studied human pathogen, Vibrio cholerae, and two other human pathogens, V. vulnificus and V. parahaemolyticus, as well as some less thoroughly characterized animal pathogens. Virulence is based on a wide diversity of mechanisms involved in motility and host colonization, in ability to persist and develop, and in damage generation. Polysaccharides may play major roles in virulence and are major components of extracellular polymeric matrix synthesized upon biofilm growth. They may also exhibit biological features, especially those similar to animal-derived... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62605/66965.pdf |
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| Cheret, Romuald; Chapleau, Nicolas; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie. |
| High pressure is an innovative non-thermal food-preservation technology. We studied the effect of high-pressure treatment up-to 500 MPa for 5 min on physical characteristics of sea bass fillets after 0, 7, and 14 d of refrigerated storage.. Color results exhibited an increase of lightness and a slight change of hue, which might be imperceptible in cooked fish. High-pressure treatment induced a decrease of exudation and water-holding capacity Pressure treatment above 300 MPa provoked higher fish hardness after storage than in untreated sample, proving the ability of high pressure to improve textural, quality of chilled, stored fish fillet. These assessments were corroborated with microstructure observations. We showed that high-pressure treatment at 500 MPa... |
| Tipo: Text |
Palavras-chave: Preservation; Microstructure; Texture profile analysis; High pressure; Fish. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-734.pdf |
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| Cheret, Romuald; Hernandez Andres, Aránzazu; Delbarre Ladrat, Christine; De Lamballerie, Marie; Verrez-bagnis, Veronique. |
| Contrary to other preservation methods like thermal treatments, high pressure can destroy microorganisms without affecting the nutritional quality, color, or food texture. The firm texture of fish flesh is an important quality parameter. During the refrigerated storage, the tissue becomes softer and the muscle is deteriorated by different proteases. The aim of this study was to study the modification of the fish muscle proteins after high-pressure treatment during the refrigerated storage and to evaluate the effect of high-pressure treatment level on the post-mortem protein changes and enzyme activities. The calpain activity decreased with the high-pressure treatment and evolved differently during the refrigerated storage, depending on the level of... |
| Tipo: Text |
Palavras-chave: Cathepsins; Calpains; Sarcoplasmic proteins; Myofibrillar proteins; Post mortem denaturation; High pressure; Fish. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1708.pdf |
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| Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel. |
| The effects of a milli(m)-calpain isolated from the white muscle of sea bass (Dicentrarchus labrax L) and commercial cathepsins B, D and L, used in combination on the myofibrillar and sarcoplasmic proteins were examined. Protein digestion was first performed by the endogenous m-calpain, (luring 2 h before the addition of a mixture of cathepsins B, D and L and a further incubation up to 22 h. Calpain degraded a 27 kDa sarcoplasmic component as well as myosin heavy chain, a-actinin, desmin and a 32 kDa component from the myofibrillar fraction. A 97 kDa component and the assumed creatine kinase-aldolase doublet were degraded during the incubation of sarcoplasmic proteins with the cathepsin mixture while, among the myofibrillar proteins, myosin, actin,... |
| Tipo: Text |
Palavras-chave: Postmortem aging; Fish muscle; Proteolysis; Cathepsins; Neutral calcium dependent protease. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-1682.pdf |
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| Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella. |
| Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show... |
| Tipo: Text |
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| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf |
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| Cheret, Romuald; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie. |
| Calpains (calcium-activated neutral proteases) of sea bass (Dicentrarchus labrax L.) muscle may participate in the degradation of muscle tissue during postmortem storage. These enzymes are regulated by calpastatin, their endogenous specific inhibitor. The objective of this study was to evaluate the changes encountered by the calpain system during the postmortem storage of fish muscle after high-pressure treatment. From 100 MPa, high-pressure treatment of purified calpains results in a loss of their activity as well as in the dissociation of the heterodimeric form. In muscle, the high-pressure processing decreases the initial activity of calpain. This loss in activity may be due to an inactivation by a change of structure. Initial calpastatin activity is... |
| Tipo: Text |
Palavras-chave: Postmortem denaturation; High pressure; Fish; Calpain system. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2786.pdf |
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| Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric. |
| Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of... |
| Tipo: Text |
Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf |
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| Calvo Dopico, D.; Mendes, R.; Silva, H.a.; Verrez-bagnis, Veronique; Pérez-martín, R.; Sotelo, C.g.. |
| This study aims to investigate the benefits associated with traceability and its relationship to quality. Market research has been conducted in Spain, Portugal, France, UK and Germany. The results show that the expected benefits for consumers related to traceability focus on food safety and quality. Relevant information about traceability should focus on the intrinsic quality attributes highly valued by consumers and food safety. Among them, it highlights the origin, the value of which has been highly appreciated by most consumers in different countries. As regards signalling, consumers rate labelling very favourably, which would have to offer all relevant information on the intrinsic quality attributes. The implementation of technology (e.g. QR) could... |
| Tipo: Text |
Palavras-chave: Labelling; Signal; Consumer behaviour; Traceability; Quality. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00349/46017/46901.pdf |
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| Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry. |
| Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one... |
| Tipo: Text |
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares). |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf |
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| Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie. |
| Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions. |
| Tipo: Text |
Palavras-chave: Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00189/30020/28607.pdf |
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| Bautista, José M.; Verrez-bagnis, Veronique; Kourti, Naouma. |
| Taxonomy and systematics of most animal species have been described in sufficient detail to permit the classification of practically any organism and, in particular, may be of use in the identification of the species and subspecies for the fish trade and ecological concerns regarding fisheries. However, before FishTrace started there was a lack of fast reference tools that hindered the efficient identification and differentiation of teleosts required in fisheries management, biological and ecological research, and human consumption. This problem could affect several of the socio-economic activities related to Fisheries Policies and therefore, the constitution of a database for identification of fish species of commercial, ecological and zoological interest... |
| Tipo: Text |
Palavras-chave: Database; Fish; Genetics. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/rapport-6595.pdf |
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| Verrez-bagnis, Veronique. |
| La récupération mécanique de pulpe de poisson à partir des résidus de filetage permet d’augmenter de 6 à 10 % le rendement en chair utilisable du cabillaud Cadus morhua. Mais pendant l'entreposage congelé, les proteines de la pulpe se dénaturent très rapidement, aussi l'emploi de substances (qu'on appelle cryoprotecteurs) qui ralentiraient, retarderaient ou inhiberaient partiellement la dénaturation des protéines a-t-il été envisagé. Afin de profiter des divers mécanismes d'action de chaque agent, il a paru intéressant de tester des mélanges de substances cryoprotectrices. |
| Tipo: Text |
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| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/00137/24868/22956.pdf |
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| Registros recuperados: 32 | |
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