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	Provedor de dados BJM (9) Naturalis (3) Genet. Mol. Biol. (3) Colegio de Postgraduados (2) Electron. J. Biotechnol. (2) PAB (2) Agronomy (1) JBAG (1) ArchiMer (1) BABT (1) BJMBR (1) Rev. Bras. entomol. (1) RChHN (1) Mais... Autor Chiquito Contreras, Roberto Gregorio (2) Rumjanek,Norma Gouvêa (2) Agudelo,V (1) Almerão,Maurício Pereira (1) Andrades,Michael (1) Araujo,Paula Beatriz (1) Araújo,Elza Fernandes de (1) Araújo,Welington Luiz (1) Azevedo,João Lúcio (1) Bakhrouf, Amina (1) Baptista,Miriam Josefina (1) Barata,José Maria Soares (1) Barros,Irene de Araújo (1) Bertoluci,Marcello Casaccia (1) Bonnet, D. (1) Borges, Wardsson Lustrino (1) Borges,Arnaldo Chaer (1) Bouchon,Didier (1) CASTILLO,GABRIELA (1) Camacho, Ana Isabel (1) Mais... Palavra-chave 16S rDNA (28) Antagonism (3) 42.74 (2) ADNr16S (2) Agroecosistemas Tropicales (2) Antagonismo (2) Bacteria (2) Bacterial diversity (2) Colonizacion micorrízica (2) Doctorado (2) Mycorrhizal colonization (2) Phytophthora parasitica (2) Pseudomonas putida (2) 12S rDNA (1) AP-PCR (1) ARDRA (1) Actinobacteria (1) Antimicrobial activity (1) Artemia (1) Azospirillum amazonense (1) Mais... Tipo do documento Info:eu-repo/semantics/article (19) Article / Letter to the editor (3) Journal article (3) Text (1) Ano 2020 (1) 2018 (1) 2017 (1) 2015 (3) 2014 (1) 2013 (1) 2012 (3) 2011 (3) 2010 (1) 2009 (2) 2008 (3) 2007 (2) 2006 (1) 2005 (1) 2003 (1) 2002 (1) 2001 (1) 2000 (1) Mais... País Brazil (18) Chile (3) Netherlands (3) Mexico (2) Argentina (1) France (1) Mais... Idioma Inglês (20) Português (3) Espanhol (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 28 Primeira ... 1 2 ... Última Rizobacterias y hongos micorrizógenos arbusculares como alternativa biotecnológica para mejorar el vigor y sanidad de portainjertos de cítricos. Colegio de Postgraduados Chiquito Contreras, Roberto Gregorio. El aprovechamiento de agentes biológicos como rizobacterias y hongos micorrizógenos arbusculares constituye una alternativa viable para disminuir el uso de agroquímicos en la producción agrícola. Se evaluó el potencial de rizobacterias y hongos micorrizógenos arbusculares como promotores del crecimiento y agentes de control biológico en plantas de cítricos. En la primera etapa de tres, se realizaron pruebas de reacción bioquímica, las cuales permitieron la identificación de las cepas FCA-56 y FCA-60 como Pseudomonas putida, FCA-8 como Pseudomonas fluorescens y en el caso de las cepas FCA-9, FCA-10, FCA-35 y FCA-52 se confirmó el género Pseudomonas. Además, se determinó la capacidad antagónica in vitro de las siete cepas rizobacterianas sobre el crecimiento... Palavras-chave: Pseudomonas putida; Phytophthora parasitica; Antagonismo; ADNr16S; Colonizacion micorrízica; Antagonism; 16S rDNA; Mycorrhizal colonization; Agroecosistemas Tropicales; Doctorado. Ano: 2011 URL: http://hdl.handle.net/10521/545 Rizobacterias y hongos micorrizógenos arbusculares como alternativa biotecnológica para mejorar el vigor y sanidad de portainjertos de cítricos. Colegio de Postgraduados Chiquito Contreras, Roberto Gregorio. El aprovechamiento de agentes biológicos como rizobacterias y hongos micorrizógenos arbusculares constituye una alternativa viable para disminuir el uso de agroquímicos en la producción agrícola. Se evaluó el potencial de rizobacterias y hongos micorrizógenos arbusculares como promotores del crecimiento y agentes de control biológico en plantas de cítricos. En la primera etapa de tres, se realizaron pruebas de reacción bioquímica, las cuales permitieron la identificación de las cepas FCA-56 y FCA-60 como Pseudomonas putida, FCA-8 como Pseudomonas fluorescens y en el caso de las cepas FCA-9, FCA-10, FCA-35 y FCA-52 se confirmó el género Pseudomonas. Además, se determinó la capacidad antagónica in vitro de las siete cepas rizobacterianas sobre el crecimiento... Palavras-chave: Pseudomonas putida; Phytophthora parasitica; Antagonismo; ADNr16S; Colonizacion micorrízica; Antagonism; 16S rDNA; Mycorrhizal colonization; Agroecosistemas Tropicales; Doctorado. Ano: 2011 URL: http://hdl.handle.net/10521/545 A microbial community analysis of the octocoral Eunicea fusca Electron. J. Biotechnol. Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G. While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and α-Proteobacteria... Tipo: Journal article Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015 A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms Electron. J. Biotechnol. Revelo Romo,Dolly Margot; Grosso,María Victoria; Moreno Solano,Nubia Carmenza; Montoya Castaño,Dolly. A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating... Tipo: Journal article Palavras-chave: 16S rDNA; Colony PCR; Degenerate primers; PHA synthase; Polyhydroxyalcanoate; Seminested PCR. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002 Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots Genet. Mol. Biol. Souza,Suzane A.; Xavier,Adelica A.; Costa,Márcia R.; Cardoso,Acleide M.S.; Pereira,Marlon C.T.; Nietsche,Silvia. The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences.... Tipo: Info:eu-repo/semantics/article Palavras-chave: ARDRA; Bacillus sp.; NifH gene; Rep-PCR; 16S rDNA. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200016 Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing Genet. Mol. Biol. Soares-Ramos,Juliana R.L.; Ramos,Humberto J.O.; Cruz,Leonardo M.; Chubatsu,Leda S.; Pedrosa,Fábio O.; Rigo,Liu U.; Souza,Emanuel M.. Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. rubrisubalbicans (M4) by restriction fragment length polymorphism (RFLP) using HindIII or DraI restriction endonucleases, random amplified polymorphic DNA (RAPD), and partial sequencing of 16S rDNA. The results of these analyses ascribed the strains studied to three distinct groups: group I, consisting of M2 and M4; group II, of ZA69; and group III, of ZA95, Z78, Z67, and Z152. RAPD fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Herbaspirillum; RAPD; RFLP; Phylogeny; 16S rDNA. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400019 Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats Genet. Mol. Biol. Falcon,Tiago; Foletto,Kelly Carraro; Siebert,Marina; Pinto,Denise Entrudo; Andrades,Michael; Bertoluci,Marcello Casaccia. Abstract The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the... Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rDNA; Deep sequencing; Saccharin; Cyclamate; Yogurt. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100805 Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii ArchiMer Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel. Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La.... Tipo: Text Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf Morphological and molecular characterization of Isostichopus sp. in the Colombian Caribbean sea JBAG Vergara,W; Agudelo,V; Castro,L; R. Rodríguez,A; Eeckhaut,I. Isostichopus sp. (Aspidochirotida: Stichopodidae) are sea cucumbers widely distributed in the Caribbean Sea. Amongst them, Isostichopus badionotus is one of the most harvested species. It shows a wide range of morphotypes widespread in the Caribbean region including different habitats (muddy, sandy and rocky bottoms, and sea grass beds). In Colombia, three morphotypes can be distinguished; two of them live in sea grass beds while the third one is found on rocky substrates. The present study describes the morphological characteristics of these morphotypes and analyzes their genetic structures through 16S rDNA and COI data. Our phylogenetic analyses show that the morphotype living on rocky substrates is morphologically and genetically distinct from the two... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucumbers; COI; 16S rDNA; Isostichopus badionotus. Ano: 2018 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000200004 Barriers in the pelagic: population structuring of Calanus helgolandicus and C. euxinus in European waters Naturalis Yebra, L.; Bonnet, D.; Harris, R.P.; Lindeque, P.K.; Peijnenburg, K.T.C.A.. Molecular studies of marine plankton have shown that ecological and/or environmental barriers play an important role in separating populations. Calanoid copepods are central in marine ecosystems, and dramatic biogeographical shifts in copepod assemblages associated with recent climate warming have been reported. We examined spatial population structuring in European waters of the Atlantic Ocean and Mediterranean Sea of Calanus helgolandicus and its sister species, C. euxi nus, from the Black Sea based on genetic and morphometric characters. The aims were to identify barriers to dispersal, relate these to hydrographic characteristics and infer historical patterns of distribution and demography. We analysed a 408 bp fragment of the mitochondrial 16S gene... Tipo: Article / Letter to the editor Palavras-chave: Calanus helgolandicus; Calanus euxinus; 16S rDNA; Mediterranean Sea; North Atlantic Ocean; Black Sea; 42.74. Ano: 2011 URL: http://www.repository.naturalis.nl/record/470574 Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments Naturalis Klossa-Kilia, E.; Kilias, G.; Sfenthourakis, S.. We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the... Tipo: Article / Letter to the editor Palavras-chave: Oniscidea; Greece; MtDNA; RLFPs; Genetic isolation; Ligidium; Isopods; 16S rDNA; 12S rDNA; 42.74. Ano: 2005 URL: http://www.repository.naturalis.nl/record/209650 A note on the systematic position of the Bathynellacea (Crustacea, Malacostraca) using molecular evidence Naturalis Camacho, Ana Isabel; Rey, Isabel; Dorda, Beatriz A.; Machordom, Annie; Valdecasas, Antonio G.. Molecular data for the mt 16S rDNA gene fragment of a bathynellacean is here presented for the first time and used to analyze the relationship of the group within the crustacean class Malacostraca (Arthropoda, Bathynellacea). Two contrasting views have classified the bathynelids as being cither within the order Syncarida or in a separate super-order Podophallocarida belonging to the infra-class Eonomostraca, a disagreement based mainly on debates over external and internal morphology. The preliminary analyses offered here in question the placement of this Bathynellacea within the Syncarida, and suggest the need for a further study of relationships among the malacostracan groups. Tipo: Article / Letter to the editor Palavras-chave: Systematics; Crustacea; Malacostraca; Bathynellacea; 16S rDNA. Ano: 2002 URL: http://www.repository.naturalis.nl/record/534365 Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do Cerrado em caupi PAB Zilli,Jerri Édson; Valicheski,Romano Roberto; Rumjanek,Norma Gouvêa; Simões-Araújo,Jean Luis; Freire Filho,Francisco Rodrigues; Neves,Maria Cristina Prata. O objetivo deste trabalho foi avaliar as relações filogenéticas de estirpes de Bradyrhizobium e a contribuição destas estirpes para a fixação biológica de nitrogênio em caupi, em solos do Cerrado. Na avaliação da relação filogenética, o gene 16S rDNA de cada uma das estirpes foi amplificado e seqüenciado, e para a análise da eficiência simbiótica, determinou-se: N total, matéria seca das plantas, massa de nódulos e redução de acetileno, em casa de vegetação, e ocupação nodular, em experimento de campo. A maioria das estirpes estudadas pertence a B. elkanii e, pelo menos dez das estirpes, independentemente da espécie, apresentaram bom desempenho quanto à fixação biológica de N2. As estirpes BR3262, BR3280 (caracterizadas como B. elkanii) e BR3267, BR3287 e... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fixação biológica de nitrogênio; 16S rDNA; Bradyrhizobium; Bradyrhizobium elkanii. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500013 Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação PAB Passos,Samuel Ribeiro; Reis Junior,Fábio Bueno dos; Rumjanek,Norma Gouvêa; Mendes,Ieda de Carvalho; Baptista,Miriam Josefina; Xavier,Gustavo Ribeiro. O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana... Tipo: Info:eu-repo/semantics/article Palavras-chave: Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; 16S rDNA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700012 Análise das relações taxonômicas e sistemáticas entre espécies de triatomíneos (Hemiptera, Reduviidae) de colônias mantidas pelo Serviço Especial de Saúde de Araraquara, inferida de seqüências do 16S rDNA mitocondrial Rev. Bras. entomol. Ceretti Junior,Walter; Vendrami,Daniel Pagotto; Gil,João Molina; Barata,José Maria Soares; Marrelli,Mauro Toledo. Foram analisadas seqüências de nucleotídeos do gene 16S do rDNA mitocondrial em 14 populações de triatomíneos mantidos em colônias no insetário SESA de Araraquara- SP, comparando-as com seqüências do mesmo gene disponíveis no GenBank. Os fragmentos variaram de 311 a 317 pb com baixa variação intra-específica entre as distâncias genéticas (0% a 0,6%), exceto para os relacionamentos entre espécimes de Triatoma sordida (1%) e espécimes de T. brasiliensis (1,3%) atribuídos a populações geográficas diferentes. A parafilia de Rhodniini e do gênero Panstrongylus foi evidenciada pelas analises, confirmando resultados anteriores entre estes e os estreitos relacionamentos de R. prolixus com R. robustus e de T. infestans e T. platensis. O relacionamento entre T.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Triatominae; Vetores da doença de Chagas; Sistemática; 16S rDNA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262008000300021 Bacterial diversity in the sediments of a temperate artificial lake, Rapel reservoir RChHN DORADOR,CRISTINA; CASTILLO,GABRIELA; WITZEL,K. P.; VILA,IRMA. Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques and molecular biology tools. Our results showed significant temporal variation in the physical and chemical composition of the water column, but no depth-related differences during the study period. To detect temporal changes in bacterial composition, cultivable heterotrophic bacteria, heterotrophic iron oxidizing bacteria, and sulfate-reducing bacteria were extracted from the surface sediments and their concentration measured. Microbial diversity in sediments was... Tipo: Journal article Palavras-chave: Sediments; Sulfate-reducing bacteria; 16S rDNA. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000200007 Rhizobial diversity from stem and root nodules of Discolobium and Aeschynomene Agronomy Martins, Claudia Miranda; Borges, Wardsson Lustrino; Costa Júnior, José de Souza; Rumjanek, Norma Gouvêa. Among the genera that exhibit stem nodulation, Aeschynomene and Discolobium are found in the Pantanal Mato-grossense (wetlands in the state of Mato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllum and A. fluminensis were collected from various locations in the Pantanal de Poconé sub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobium and A. fluminensis were observed. Among... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Agrárias; Ciência do Solo Flooding-tolerant legume; Symbioses; Pantanal; 16S rDNA; Biological nitrogen fixation; PCR-RFLP Microbiologia do Solo. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19565 Phytase-Producing Bacteria from Extreme Regions in Indonesia BABT Sajidan,; Wulandari,Rita; Sari,Evy Novita; Ratriyanto,Adi; Weldekiros,Hailu; Greiner,Ralf. ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal... Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rDNA; Bacillus sp. bacterial phytase phytate phytate-degrading enzyme. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711 Partial characterization of nif genes from the bacterium Azospirillum amazonense BJMBR Potrich,D.P.; Passaglia,L.M.P.; Schrank,I.S.. Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum amazonense; Nif genes; GlnB; 16S rDNA. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900002 The use of 16s rDNA methods in soil microbial ecology BJM Macrae,Andrew. New and exciting molecular methods, many using the 16S small sub-unit ribosomal nucleic acid molecule, are opening the microbial "black box" in soil. These studies have added much to our knowledge of microbial diversity in soils, and are beginning to advance our understanding of the relationship between this diversity and its function in soil processes. Over the next few years, the knowledge gained from molecular studies will, we hope, lead to improvements in sustainable land management and sustainable exploitation of soil genetic resources. As we enter the third millenium, it is appropriate to review the application of 16S rDNA methods to soil microbiology. This review examines 16S ribosomal DNA (rDNA) methods and their application to soil. It mentions... Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil; Bacteria; Ecology; 16S rDNA. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200002 Registros recuperados: 28 Primeira ... 1 2 ... 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