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	Provedor de dados ArchiMer (1) BABT (1) Autor Alegre,Ranulfo Monte (1) Alsberg, Tomas (1) Anschau,Andréia (1) Charlou, Jean-luc (1) Holm, Nils G (1) Konn, Cecile (1) Magner, Jörgen (1) Santos,Lucielen Oliveira dos (1) Mais... Palavra-chave Amino Acids (2) Fed Batch Culture (1) Fermentation (1) Glutathione (1) Hydrothermal Fluids (1) Ion-Pairing (1) Mass Spectrometry (1) QTOF (1) Response Surface Methodology (1) UPLC (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Text (1) Ano 2015 (1) 2013 (1) País Brazil (1) France (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol BABT Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte. This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017 A Method for Detection of Trace Concentrations of Underivatized Amino Acid in Hydrothermal Fluids by Ion-Pairing Reversed-Phase UPLC-ESI-QTOF-MS ArchiMer Konn, Cecile; Magner, Jörgen; Charlou, Jean-luc; Holm, Nils G; Alsberg, Tomas. Investigation of amino acids in hydrothermal systems is of prime importance for the understanding of geochemistry and microbiology of hydrothermal vents and plumes, for carbon and metals global cycles, for metabolism of some hydrothermal microorganisms and for the origin of life issue. Extensive theoretical and experimental work on amino acids behaviour in hydrothermal fluids has been done, conversely only few data exist on natural samples. Because each hydrothermal vent is unique, the more data we collect the better we will be able to address each of these questions. Usually amino acids in hydrothermal fluids have been measured by HPLC-FLD. The chromatographic separation was at least 26 min and up to 135 min and the required derivatization step may be... Tipo: Text Palavras-chave: Amino Acids; UPLC; Ion-Pairing; QTOF; Mass Spectrometry; Hydrothermal Fluids. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00271/38188/36339.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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