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Nutritional Requirements for Growth of Agaricus brasiliensis - doi: 10.4025/actascibiolsci.v33i1.7010 3
Gaspar Júnior, Pascoal José; Centro Universitário de Formiga - UNIFOR-MG; Tomizawa, Márcia Mayumi; Universidade Federal de Lavras; Schwan, Rosane Freitas; Universidade Federal de Lavras; Rinker, Danny Lee; University of Guelph; Dias, Eustáquio Souza; Universidade Federal de Lavras, Departamento de Biologia, Setor de Microbiologia..
The nutritional requirements of A. brasiliensis in culture media were assessed by supplementing a basal medium (g L-1): (glucose, 10, KH2PO4, 1, MgSO4.7H2O, 0.5, [NH4]2SO4, 1, pH 5.5) with CaCl2, trace elements (FeSO4.7H2O; MnCl2.4H2O; ZnSO4.7H2O; CuSO4.5H2O), casein, yeast extract, peptone, B-vitamins or amino acids. Evaluations were based on the mycelial growth in solid or liquid culture (mm day-1 or mg day-1) and visual analysis of the colony. The addition of CaCl2 and trace elements was very important for the major mycelial growth of the fungi. The addition of casein and inositol to the medium did not have a significant effect on growth. The best growth result in solid medium was obtained with the basal medium plus the addition of yeast extract and...
Tipo: Crescimento micelial, exigências nutricionais Palavras-chave: 2.12.00.00-9 Mushroom; Trace elements; B-vitamins; Amino acids Microbiologia Mushroom; Trace elements; B-vitamins; Amino acids.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7010
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Comparative study on the integrated application of environmental friendly compounds and a chemical- nematicide in controlling root knot nematode Meloidogyne incognita infecting sunflower plants; a field study 111
Mohamed, Moawad.
A field study was set up at Kafre – Hakim village, Giza, Governorate, Egypt, to compare the protective value of applying environmental friendly compounds with a known chemical nematicide, oxamyl, for controlling the nematode Meloidogyne incognita infecting sunflower cv. Sakha 53  and their effects on crop production and oil contents. These compounds were 1) furfural,a natural nematicide, 2) amino green 11, commercial formula of amino acids, 3) NPK, an inorganic fertilizer. Data revealed that all the tested treatments resulted in variable significant decrease in root knot disease incidence associated with improvement in crop yield except the plants treated with furfural plus amino green 11 that showed significant decrease in percentage of oil content. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Root-knot nematode; Meloidogyne incognita; Amino acids; Furfural; Oxamyl; Eggplant..
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4422
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Crambe (Crambe abyssinica) and sunflower (Helianthus annuus) protein concentrates: production methods and nutritional properties for use in fish feed 42
LOVATTO,NAGLEZI M.; GOULART,FERNANDA R.; LOUREIRO,BRUNO B.; SPERONI,CAROLINE S.; BENDER,ANA B.B.; GIACOMINI,SANDRO J.; RADÜNZ NETO,JOÃO; SILVA,LEILA P. DA.
ABSTRACT The purpose of this study was to develop and improve protein concentration techniques for two industrial by-products with the potential for use in fish feed. In particular, we chemically characterized crambe meal and sunflower meal and their protein concentrates. Three different protein concentration methods were tested: isoelectric pH (pHi), acid pH and alkaline pH. For crambe and sunflower meals extraction using the pHi method was most efficient in terms of protein yield and crude protein content in the concentrates; this method also increased lysine and methionine content in the concentrates. The water holding capacity of the sunflower protein concentrate was greater than that of the crambe protein concentrate. The crambe protein concentrate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Antinutrients; Aquafeeds; Byproducts; Phenolic compounds; Protein concentration..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502495
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Influence of low-protein diet with different levels of amino acids on laying hen performance, quality and egg composition 42
ALAGAWANY,MAHMOUD; EL-HINDAWY,MOHAMED M.; EL-HACK,MOHAMED E. ABD; ARIF,MUHAMMAD; EL-SAYED,SABRY ABD.
Abstract The present study aimed to investigate the consequences of dietary levels of crude protein (CP) and total sulfur amino acids (TSAA, Met+Cys) on productive performance, egg quality and egg components for Lohmann Brown laying hens through 18-34 weeks of age. A 2×3 factorial design experiment was conducted using two levels of CP (16 and 18 %) and three levels of Met+ Cys (0.67, 0.72 and 0.77 %). A total of 144 Lohmann Brown laying hens at 18 weeks of age were randomly divided into 6 treatment groups. The best values of final body weight and body weight change were recorded by the hens fed 18 % CP diet. Increasing dietary CP up to 18 % accompanied with the best (P> 0.01) feed conversion, egg weight and egg mass. The combination between low-CP diet...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Layers; Low-protein diet; Amino acids; Production; Egg quality; Blood.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100712
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Timing carbon turnover (δ13C) in weaned piglet’s brain by IRMS 42
SALEH,MAYRA A.D.; SANTOS,LUAN S. DOS; BERTO,DIRLEI A.; AMORIM,ALESSANDRO B.; COSTA,VLADIMIR E.; DUCATTI,CARLOS.
Abstract Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (δ13C) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additives; Amino acids; Isotopic dilution; Stable isotopes; Swine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502469
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Growth, Yield and Grain Nutritional Quality in Three Brazilian Pearl Millets (Pennisetum americanum L.) with African or Indian origins 42
MEDICI,LEONARDO O.; GONÇALVES,FABÍOLA V.; FONSECA,MARCOS PAULO S. DA; GAZIOLA,SALETE A.; SCHMIDT,DAIANA; AZEVEDO,RICARDO A.; PIMENTEL,CARLOS.
ABSTRACT In this study, we are presenting recommendations to the best agricultural use as well as for plant breeding of three millet cultivars namely ENA1 and ENA2, which have African origin, and BRS1501 originally from India. These cultivars were evaluated for growth, yield and grain quality traits. The morphological traits evaluated in this study indicated that the African genotypes ENA1 and ENA2 are better than the Indian genotype BRS1501 for no-till farming or to produce forage with 15% of crude protein at flowering and at harvest to produce stover (around 7% of crude protein content) for livestock feeding. The BRS1501 cultivar exhibited the highest values for total crude protein, albumins and prolamins, phytate and mineral contents in grains. ENA1 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mineral density; SDS-PAGE; Phytate; Seed storage proteins; Amino acids.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401749
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Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming 42
ALCÂNTARA,BERENICE K.; RIZZI,VANESSA; GAZIOLA,SALETE A.; AZEVEDO,RICARDO A..
ABSTRACT Both the scientific community and society have shown interest in improving the content of amino acids, carbohydrates and mineral nutrients in maize because it represents an important staple food in many developing countries. Earlier studies demonstrated that the treatment of seeds using ascorbic acid (AsA-seed priming) enhanced soluble carbohydrates, proteins and soluble amino acids for other species. AsA seed priming in maize showed the potential for reducing abiotic stresses. The effects on grain quality have not been previously demonstrated. This study investigated the impacts of AsA seed priming on maize kernel quality of seeds produced by the plants generated from the primed seeds, based on the amino acid profile and carbohydrate and mineral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitamin C; Grain quality; Boron; Sugar; Amino acids.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200695
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Performance and egg quality of laying hens fed with dietary levels of digestible lysine and threonine 45
Figueiredo,G.O; Bertechini,A.G; Fassani,E.J; Rodrigues,P.B.; Brito,J.Á.G; Castro,S.F.
The present study was developed using 864 Hy-Line W36 laying hens (42 to 58 weeks of age) with a randomized experimental design in a 4x3 factorial arrangement, with five replicates and 12 birds per experimental unit and 4 evaluation periods of 28 days each. The digestible lysine levels studied were 0.675%, 0.743%, 0.811% and 0.879%, and the digestible threonine levels were 0.542%, 0.596% and 0.650%. The performance and egg quality was evaluated. There were no significant effects on the egg production, specific gravity, albumen and yolk percentage and total solids in albumen and yolk for birds submitted to the different dietary digestible lysine and threonine levels. There was significant interaction between these amino acids on the egg shell percentage and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Laying hens; Crude protein; Haugh unit.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300028
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Role of Natural Antioxidants in the Modulation of Plasma Amino Acid Pattern in Rats Exposed to Hemic Hypoxia 52
Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Attia,Hala; Ali,Hanaa Mahmoud; El-Agami,Hanaa.
ABSTRACTThe aim of this work was to investigate whether the free radical scavengers, L-arginine (L-arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer's ratio [concentrations of branched chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer's ratios and increased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L-arg, (200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia ,...
Palavras-chave: Natural antioxidants; Amino acids; Carnosine; L-arginine; Fisher's ratio; Urea.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500741
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Alterations in proteins and amino acids of the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense in response to industrial wastewater pollution 52
El-Sheekh,Mostafa Mohamed; El-Otify,Ahmed Mohamed; Saber,Hani.
The effect of industrial wastewater on the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense was investigated. The data showed that P. limnetica was more sensitive to pollution than A. wisconsinense. The treatments with different levels of wastewater exerted pronounced reductions in protein and amino acids content. SDS-PAGE analysis revealed that the cyanobacteria grown in the industrial wastewater showed induction in the synthesis of certain polypeptides and repression of others. The treatments of P. limnetica with wastewater stimulated the appearance of six protein bands with molecular masses of 28, 30, 31, 32, 58 and 97 kDa. The same treatments caused the disappearance of 20, 38 and 56 kDa. The structural protein pattern of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Anabaena; Electrophoretic patterns; Nile cyanobacteria; Protein; Pseudanabaena; Wastewater.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400022
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Compositional changes of proteins and amino acids in germinating coffee seeds 52
Shimizu,Milton Massao; Mazzafera,Paulo.
Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Coffea arabica; Endosperm; Germination; Protein; Seeds.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300003
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Are high-lysine cereal crops still a challenge? 56
Ferreira,R.R.; Varisi,V.A.; Meinhardt,L.W.; Lea,P.J.; Azevedo,R.A..
The essential amino acids lysine and threonine are synthesized in higher plants via a pathway starting with aspartate that also leads to the formation of methionine and isoleucine. Lysine is one of most limiting amino acids in plants consumed by humans and livestock. Recent genetic, molecular, and biochemical evidence suggests that lysine synthesis and catabolism are regulated by complex mechanisms. Early kinetic studies utilizing mutants and transgenic plants that over-accumulate lysine have indicated that the major step for the regulation of lysine biosynthesis is at the enzyme dihydrodipicolinate synthase. Despite this tight regulation, recent strong evidence indicates that lysine catabolism is also subject to control, particularly in cereal seeds. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lysine; Threonine; Aspartate; Nutritionally improved crops; Methionine; Amino acids.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700002
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Thyroid peroxidase activity is inhibited by amino acids 56
Carvalho,D.P.; Ferreira,A.C.F.; Coelho,S.M.; Moraes,J.M.; Camacho,M.A.S.; Rosenthal,D..
Normal in vitro thyroid peroxidase (TPO) iodide oxidation activity was completely inhibited by a hydrolyzed TPO preparation (0.15 mg/ml) or hydrolyzed bovine serum albumin (BSA, 0.2 mg/ml). A pancreatic hydrolysate of casein (trypticase peptone, 0.1 mg/ml) and some amino acids (cysteine, tryptophan and methionine, 50 µM each) also inhibited the TPO iodide oxidation reaction completely, whereas casamino acids (0.1 mg/ml), and tyrosine, phenylalanine and histidine (50 µM each) inhibited the TPO reaction by 54% or less. A pancreatic digest of gelatin (0.1 mg/ml) or any other amino acid (50 µM) tested did not significantly decrease TPO activity. The amino acids that impair iodide oxidation also inhibit the TPO albumin iodination activity. The inhibitory amino...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid; Thyroperoxidase; Amino acids; Iodide oxidation; Lactoperoxidase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300015
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Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway 56
Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G..
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003
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Effect of Walker 256 tumor growth on intestinal absorption of leucine, methionine and glucose in newly weaned and mature rats 56
Gomes-Marcondes,M.C.C.; Honma,H.N.; Areas,M.A.; Cury,L..
In tumor-bearing rats, most of the serum amino acids are used for synthesis and oxidation processes by the neoplastic tissue. In the present study, the effect of Walker 256 carcinoma growth on the intestinal absorption of leucine, methionine and glucose was investigated in newly weaned and mature rats. Food intake and carcass weight were decreased in newly weaned (NT) and mature (MT) rats bearing Walker 256 tumor in comparison with control animals (NC and MC). The tumor/carcass weight ratio was higher in NT than in MT rats, whereas nitrogen balance was significantly decreased in both as compared to control animals. Glucose absorption was significantly reduced in MT rats (MT = 47.3 ± 4.9 vs MC = 99.8 ± 5.3 nmol min-1 cm-1, Kruskal-Wallis test,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Walker 256 tumor; Intestinal absorption; Amino acids; Glucose; Aging.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000017
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Elevated amniotic fluid amino acid levels in fetuses with gastroschisis 56
Kale,A.; Kale,E.; Akdeniz,N.; Canoruc,N..
Our objective was to measure maternal plasma and amniotic fluid amino acid concentrations in pregnant women diagnosed as having fetuses with gastroschisis in the second trimester of pregnancy. Twenty-one pregnant women who had fetuses with gastroschisis detected by ultrasonography (gastroschisis group) in the second trimester and 32 women who had abnormal triple screenings indicating an increased risk for Down syndrome but had healthy fetuses (control group) were enrolled in the study. Amniotic fluid was obtained by amniocentesis, and maternal plasma samples were taken simultaneously. The chromosomal analysis of the study and control groups was normal. Levels of free amino acids and non-essential amino acids were measured in plasma and amniotic fluid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Gastroschisis; Amniotic fluid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800004
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Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species 56
Tiwari,F.; Singh,D.K..
Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquarium was divided into four concentric zones. At the starting time of the assay 10 snails were placed on the circumference of outermost zone 0. Snail attractant pellets (SAP) were added simultaneously in the center of central zone 3. SAP of different amino acids were prepared at concentrations of 10, 20, 50, 80 and 100 mM/2% agar solution and, subsequently, spread to a uniform thickness of 5 mm. After cooling, SAP were cut in small pieces of 5 mm in diameter....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Snails; Lymnaea acuminata; Fasciola; Amino acids; Attraction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400016
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Effects of monosulfuron-ester on metabolic processes of nitrogen-fixing cyanobacteria Anabaena flos-aquae and Anabaena azotica 58
Shen,Jian Ying; Liao,Jin Zhi; Guo,Li Li; Su,Rui Fang.
Abstract Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300 nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC50 of 3.3 and 101.3 nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300 nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetolactate synthase; Amino acids; Cyanobacteria; Monosulfuron-ester.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300544
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Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum 58
Pinotti,T.; Carvalho,P.M.B.; Garcia,K.M.G.; Silva,T.R.; Hagler,Allen Norton; Leite,S.G.F..
The ability of Geotrichum candidum to produce fruity aroma in food grade sucrose, molasses, corn steep liquor and peptone based culture media was tested by sensory evaluation and analyzed by gas chromatography mass spectrometry. A strong and sweet fruity aroma was produced from molasses, with peptone or corn steep liquor stimulating aroma production. Molasses with peptone supplemented with leucine, valine, or alanine yielded better fruity aroma production and the presence of many esters was consistent with the fruity aroma production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Geotrichum candidum; Aroma; Corn steep liquor; Molasses; Amino acids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400017
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Lysine catabolism: flow, metabolic role and regulation 61
Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido.
Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Lysine; Metabolism; Mutants.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002
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