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Enrichment of population density of a bacterial consortium during bioremediation of a soil under successive contaminations with diesel oil Biological Sciences
Leal, Patrícia Lopes; Pinheiro Dadalto, Silvana; Fernandes, Rita de Cássia Rocha; Queiroz, Maria Eliana Lopes Ribeiro; Tótola, Marcos Rogério.
The effect of successive soil contamination with diesel oil was evaluated on population dynamics of a bacterial consortium (Acinetobacter baumannii LBBMA 04, Pseudomonas aeruginosa LBBMA 58, Ochrobactrum anthropi LBBMA 88b, Acinetobacter baumannii LBBMAES11, and Bacillus subtilis LBBMA 155) and on biodegradation of petroleum hydrocarbons (n-C12-C22). After each contamination with diesel oil, soil samples were collected for assessment of bacterial population and sequence of petroleum hydrocarbons degradation. At 20 and 40 days, the highest percentage of degradation was observed for the higher carbon chain hydrocarbons (n-C21 and C22). After the third contamination, there was a considerable reduction of n-C21degradation and a high degradation of hydrocarbons...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Agrárias; Microbiologia; Biorremediação bioaugmentation; Biodegradation; Pollution control; Hydrocarbons. Ciências Agrárias I.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36904
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Bioremediation of diesel/biodiesel contaminated sandy soil in microcosm: evaluation of fungal bioaugmentation and natural attenuation Biological Sciences
Anjos, Noelly Almeida dos; Lima, Suzy Darley de; Rothebarth, Ana Vitória de Paula; Lima, Zoraidy Marques de; Caixeta, Danila Soares; Morais, Eduardo Beraldo de.
  Biodiesel is a clean renewable fuel used as alternative energy source to diesel and it is commercialized as a minor component in diesel blends. Similarly to diesel, biodiesel spill is a source of contamination for the ecosystem making necessary to provide effective remediation strategies. Bioremediation is a technology that has been applied with success to clean up hydrocarbon-contaminated environments. In this study, fungal bioaugmentation strategy was compared with natural attenuation during bioremediation of a sandy soil contaminated with diesel, biodiesel and blends (B20 and B50). Respirometric assays simulating the contamination of soil were carried out in Bartha flasks used to measure microbial CO2 production. Penicillium sp. AV4 isolated from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Hydrocarbon; Respirometric assay; CO2; Penicillium sp. AV4..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/39468
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Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs - DOI: 10.4025/actascibiolsci.v32i1.7575 Biological Sciences
Silva, Ísis Serrano; UNICAMP; Menezes, Cristiano Ragagnin de; UNICAMP; Faria, Andréia Fonseca de; UNICAMP; Costa, Josemar Gurgel da; UFRN; Pepper, Susan; The University of Melbourne; Britz, Margaret; The University of Melbourne; Durrant, Lucia Regina; UNICAMP.
Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7575
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Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 Anais da ABC (AABC)
ZHU,JIANGWEI; ZHAO,YAN; RUAN,HONGHUA.
Abstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and biochemical characteristics. The strain CM-Z19 showed 92.6% degradation of chlorpyrifos-methyl (100 mg/L) within 5 days of incubation, and the strain CM-Z6 was 99.1% under the same conditions. In addition, the degradation characteristics of the two strains were compared and studied, and the results showed that the strain CM-Z19 had higher phosphoesterase activity and ability to degrade the organophosphorus pesticide than did the strain CM-Z6. However, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus megaterium; Pseudomonas syringae; Chlorpyrifos-methyl; 3; 5; 6-trichloro-2-pyridinol; Biodegradation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500624
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Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor BABT
Komarkova,Eliska; Paca,Jan; Klapkova,Eva; Stiborova,Marie; Soccol,Carlos R; Sobotka,Miroslav.
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenol; Biodegradation; Candida tropicalis; Phenol hydroxylase; Starvation stress; Viability.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007
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Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers BABT
Alauzet,Nathalie; Roussos,Sevastianos; Garreau,Henri; Vert,Michel.
Studies were performed to appreciate the presence of micro-organisms able to degrade OLA, in earthworms casts or in the surroundings. Worms were grown in biosynthesol, an artificial soil. The counting of bacteria and fungi in earthworms casts and in biosynthesol without earthworms suggested that earthworms ate some of the micro-organisms. The main filamentous fungi genera found were Aspergillus, Trichoderma, Fusarium and Penicillium. Previous results in the literature have shown that some species from the Aspergillus and Fusarium genera were able to degrade OLA and other aliphatic esters. It could be suggested that these two genera and some bacteria were responsible for the pre-degradation of OLA, and that earthworms might eat them.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Earthworm; Eisenia andrei; Biodegradation; Lactic acid polymers; Artificial soil; Natural microflora.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200001
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Biocompatibility, osteointegration, osteoconduction, and biodegradation of a hydroxyapatite-polyhydroxybutyrate composite BABT
Reis,Emily Correna Carlo; Borges,Andréa Pacheco Batista; Fonseca,Cláudio César; Martinez,Mastoby Miguel Martinez; Eleotério,Renato Barros; Morato,Gláucia Oliveira; Oliveira,Paulo Miranda.
In this work, biocompatibility, osteointegration, osteoconductivity, and biodegradation of a hydroxyapatite polyhydroxybutyrate new composite were evaluated. The composite was implanted in rabbits' bone defects and clinical, radiographic, histological, and histomorphometric data of these animals were compared with those of unfilled defects on the days 8th, 45th, and 90th after surgery. No significant differences existed between the groups for the evaluated clinical parameters. Radiographs showed bone-composite direct contact. Bone formed within the defect, interface and inside the composite. Significant differences were found between the bone and connective tissues percentage within the defect at all dates and at the interface on the 45th day, bone tissue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomaterials; Bone; Biocompatibility; Biodegradation; Hydroxyapatite-polyhydroxybutyrate composite.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400010
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Simple models for the continuous aerobic biodegradation of phenol in a packed bed reactor BABT
Gerrard,Andrew Mark; Páca Júnior,Jan; Kostecková,Alena; Páca,Jan; Stiborová,Marie; Soccol,Carlos Ricardo.
This paper proposes the use of a preliminary, phenol removal step to reduce peak loads arriving at a conventional effluent plant. A packed bed reactor (PBR) using polyurethane foam, porous glass and also cocoa fibres as the inert support material was used. Experiments have been carried out where the flow-rates, plus inlet and outlet phenol concentrations were measured. A simple, plug-flow model is proposed to represent the results. Zero, first order, Monod and inhibited kinetics rate equations were evaluated. It was found that the Monod model gave the best fit to the experimental data and allowed linear graphs to be plotted. The Monod saturation constant, K, is approximately 50 g m-3, and ka is around 900 s-1.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Phenol; Aerobic reactor; Models.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500018
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Selection of microorganisms degrading S-Metolachlor herbicide BABT
Martins,Paula Fabiane; Martinez,Camila Ortiz; Carvalho,Giselle de; Carneiro,Paulo Irajara Borba; Azevedo,Ricardo Antunes; Pileggi,Sônia Alvim Veiga; Melo,Itamar Soares de; Pileggi,Marcos.
The aim of this work was to study herbicide degradation through selected microorganisms from humus and soil subjected to different plantation systems. The following bacterial species were identified: Klebsiella pneumoniae pneumoniae GC s.B strain 1, Pseudomonas alcaligenes, Enterobacter aerogenes GC s.A and Klebsiella pneumoniae pneumoniae GC s.B strain 2. Growth studies yet suggested the possibility of a very long lag phase. Although, culture with the herbicide presented biofilm formation and there were color changes in the herbicide that could have interfered with the espectrophotometry readings. After 5 days of incubation at 35ºC, the difference in the concentration of herbicide was 14.42% on average and after 10 days, 35.01%.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Biodegradation; S-Metolachlor; Herbicide.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100019
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Electrolytic treatment applied to the industrial effluent containing persistent wastes monitored by Bartha respirometric assays BABT
Régis,Gisela; Bidoia,Ederio Dino.
The effluent of a rubber chemical antioxidant and antiozonant producer industry, with high content of organic material was subjected to electrolytic process. To evaluate the speed of stabilization of the eletroctrolyzed effluents, and to evaluate the biodegradation the respirometric test of Bartha and Pramer was used. The monitoring of the biodegradation of the effluent, after different periods of electrolysis show that the ideal time of electrolysis was 10 and 25 min. It was concluded that the eletrolytic process was viable to diminish the adaptation time of the microorganism to the effluent and consequently increased the biodegradation of effluent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrolytic process; Biodegradation; Wastes pollutant; Electrodes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200020
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New aspects on atrazine biodegradation BABT
Sene,Luciane; Converti,Attilio; Secchi,Geslaine Aparecida Ribeiro; Simão,Rita de Cássia Garcia.
The world practice of using agrochemicals for long periods, in an indiscriminated and abusive way, has been a concern of the authorities involved in public health and sustainability of the natural resources, as a consequence of environmental contamination. Agrochemicals refer to a broad range of insecticides, fungicides and herbicides, and among them stands out atrazine, a herbicide intensively used in sugarcane, corn and sorghum cultures, among others. Researches have demonstrated that atrazine has toxic effects in algae, aquatic plants, aquatic insects, fishes and mammals. Due to the toxicity and persistence of atrazine in the environment, the search of microbial strains capable of degrading it is fundamental to the development of bioremediation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atrazine; Biodegradation; Bacteria; Fungi; Microbial consortium.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200030
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Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco - Brazil BABT
Miranda,Rita de Cássia; Souza,Cynthia Silva de; Gomes,Edelvio de Barros; Lovaglio,Roberta Barros; Lopes,Carlos Edison; Sousa,Maria de Fátima Vieira de Queiroz.
The aim of this work was to investigate the potential of the diesel oil degrading yeasts to use them in bioremediation of areas contaminated by this pollutant. The cultures, identified as Rhodotorula aurantiaca UFPEDA 845 and Candida ernobii UFPEDA 862, were selected at the initial stage. In the course of the biodegradation assays, C. ernobii degraded tetradecane, 5 methyl-octane and octadecane completely and decane (60.8%) and nonane (21.4%) partially whilst R. aurantiaca presented degradation percentages of 93% for decane, 38.4% for nonane and 22.9% for dodecane.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Yeasts; Diesel oil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100018
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Azo Dye Mineralization by Phanerochaete Chysosporium in a Sequencing Bath Reactor BABT
Wanderley,Carlos Ronald Pessoa; Andrade,Marcus Vinícius; Pereira,Luciana José; Silva,Gloria Maria Marinho; Pessoa,Kelly Rodrigues.
ABSTRACT The mineralization of the azo dye congo red by the fungi Phanerochaete chrysosporium was studied in two sequential batch bioreactors (R1 and R2), operated in cycles of 48 h (step I) and 24 h (step II). In step I, glucose concentration was 1 g.L-1 in both reactors and in step II, 1 g.L-1 of glucose was maintained in R1, but R2 received no addition of glucose. In step I, the average dye removal efficiencies were 76 ± 29 % (R1) and 53 ± 15% (R2), while in step II the averages recorded for dye removal for R1 and R2 were 84 ± 15 and 70 ± 28%, respectively. The rates of dye removal were 0.04 h-1 in R1 and 0.03 h-1 in R2 in step I. Higher rates were obtained in step II, 0,07 h-1 and 0,02 h-1 for R1 and R2, respectively. The highest dye removal occurred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Congo red; Phanerochaete chrysosporium; Sequential bioreactors.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100606
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Evaluation of the use of vinasse as a biostimulation agent for the biodegradation of oily sludge in soil BABT
Crivelaro,Sérgio Henrique Rezende; Mariano,Adriano Pinto; Furlan,Luis Tadeu; Gonçalves,Renato Almeida; Seabra,Paulo Negrais; Angelis,Dejanira de Franceschi de.
This work aimed to study the effect of vinasse on the biodegradation in soil of oily sludge from the Replan-Petrobras oil refinery. The Bartha respirometric method was used to verify the efficiency of the treatments with soil, oily sludge (7 and 14% m/m) and soil moisture adjustment with or without vinasse (0.11 mL/g dry soil) for 121 days. Although an increase in the soil microbial population was obtained with the vinasse, it demonstrated not to be adequate to enhance the biodegradation efficiency of the oily sludge in soil, since there was no difference between the CO2 produced from treatments with or without vinasse after the complete consumption of the vinasse. Thus, the use of vinasse as an amendment to biodegradation processes showed not to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Soil; Oily sludge; Vinasse; Landfarming.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500027
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Biodegradation of crude petroleum oil and environmental pollutants by Candida tropicalis strain BABT
Farag,Soha; Soliman,Nadia A..
A local yeast isolate named A was isolated from polluted area of Abou-Qir gulf (Alexandria, Egypt), identified according to a partial sequence of 18sRNA as Candida tropicalis. The isolate showed a high potency in petroleum oil biodegradation as well some hydrocarbons. Morphological changes in cell diameter of this yeast were recognized upon growing the target cell in sea water medium supplemented with petroleum oil as sole carbon source in comparison to the growth in enriched medium. Statistically-based experimental design was applied to evaluate the significance of factors on petroleum oil biodegradation by this yeast isolate. Eleven culture conditions were examined by implementing Plackett-Burman factorial design where aeration, NH4Cl and K2HPO4 had the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Petroleum oil; Candida tropicalis; 18Sr RNA; Biodegradation; Plackett-Burman.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400023
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Catechol biodegradation kinetics using Candida parapsilopsis BABT
Rigo,Maurício; Alegre,Ranulfo Monte; Bezerra,José Raniere Mazile Vidal; Coelho,Narjara; Bastos,Reinaldo Gaspar.
This study evaluated the biodegradation of catechol by a yeast strain of Candida parapsilopsis in standard medium in Erlenmeyer flasks. Results shown that the highest concentration of catechol caused the longer lag period, demonstrating that acclimatized cultures could completely degrade an initial catechol concentration of 910 mg/L within 48 h. Haldane's model validated the experimental data adequately for growth kinetics over the studied catechol concentration ranges of 36 to 910 mg/L. The constants obtained for this model were µmax = 0.246 h-1, Ks = 16.95 mg/L and Ki = 604.85 mg/L.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida parapsilopsis; Biodegradation; Catechol; Growth kinetics; Equation of Haldane; Substrate inhibition.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200029
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Validation of computationally predicted substrates for laccase BABT
Reena,; Dhall,Purnima; Kumar,Rita; Kumar,Anil.
Present study reports the validation (oxidation) of computationally predicted oxidation of xenobiotic contaminants by commercially available pure laccase from Trametes versicolor. Selected contaminants were predicted as potential targets for laccase oxidation by using in-silico docking tool. The oxidation by laccase was measured by change in absorbance at specific λ max of each compound. Sinapic acid and tyrosine were taken as positive and negative controls, respectively. Oxidation was observed in m-chlorophenol, 2,4 di-chlorophenol, 2,4,6 tri-chlorophenol, captan, atrazine and thiodicarb, except malathion, which showed no activity. It could be speculated that the predicted substrates showing oxidation shared homology at structural and chemical level with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; In silico screening; Laccase; Xenobiotics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500803
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Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate BABT
Shalin,Thomas; Sindhu,Raveendran; Binod,Parameswaran; Soccol,Carlos Ricardo; Pandey,Ashok.
Poly-ß-hydroxybutyrate (PHB) is a biodegradable intracellular microbial product produced by many bacteria and it is comparable to some of the petrochemical derived thermoplastics such as polypropylene. One of the main barriers for the commercial exploitation is the high cost of the substrate for the production of biopolymer. The utilization of mixed microbial cultures facilitates the use of complex substrates thereby reducing the cost of PHB production. In the present study, mixed culture systems were evaluated for PHB production. Bacillus firmus NII 0830 was used for the production of PHB since it accumulates a large amount of PHB and a second organism Lactobacillus delbrueckii NII 0925 was used to provide lactic acid. FTIR and 1H NMR analyses revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly-3-hydroxybutyrate; Biopolymer; Mixed culture; Bacillus firmus; Lactobacillus delbrueckii; Biodegradation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500644
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Isolation of diuron-degrading bacteria from treated soil BABT
Dellamatrice,Priscila Maria; Monteiro,Regina Teresa Rosim.
Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Biomass; Enhanced degradation; Diuron; Acinetobacter; Bioremediation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020
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Factors influencing crude oil biodegradation by Yarrowia lipolytica BABT
Ferreira,Tatiana Felix; Coelho,Maria Alice Zarur; Rocha-Leão,Maria Helena Miguez da.
Yarrowia lipolytica is unique strictly aerobic yeast with the ability to efficiently degrade hydrophobic substrates such as n-alkenes, fatty acids, glycerol and oils. In the present work, a 2(4) full factorial design was used to investigate the influence of the independent variables of temperature, agitation, initial cell concentration and initial petroleum concentration on crude oil biodegradation. The results showed that all variables studied had significant effects on the biodegradation process. Temperature, agitation speed and initial cell concentration had positive effects, and initial petroleum concentration had a negative effect. Among the crude oil removal conditions studied, the best temperature and agitation conditions were 28ºC and 250 rpm,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yarrowia lipolytica; Crude oil; Biodegradation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500019
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