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Effects of aroma quality on the biotransformation of natural 2-phenylethanol produced using ascorbic acid Electron. J. Biotechnol.
Tian,Xun; Ye,Rui; Wang,Jingwen; Chen,Yifei; Cai,Baoguo; Guan,Shimin; Rong,Shaofeng; Li,Qianqian.
Background Natural 2-phenylethanol (2-PE) is an important flavoring that emits the aroma of roses. During biotransformation, the aroma quality of natural 2-PE is affected by its main by-products, which include butanol, isobutyric acid, butyric acid, and isovaleric acid. Thus, controlling undesirable by-product formation can reduce the effect of odor on 2-PE aroma quality. Results 2-PE was produced through biotransformation using l-phenylalanine as a substrate and glucose as a carbon source. Ascorbic acid was added to the system to improve the redox reaction and suppress the generation of by-products. Principal component analysis of the aroma quality of 2-PE was performed using an electronic nose. Similarity analysis revealed that the effects of four...
Tipo: Journal article Palavras-chave: Aroma quality; Ascorbic acid; Biotransformation; By-products; 2-Phenylethanol.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400005
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Bioconversion of (+)- and (-)-alpha-pinene to (+)- and (-)-verbenone by plant cell cultures of Psychotria brachyceras and Rauvolfia sellowii Electron. J. Biotechnol.
Limberger,Renata Pereira; Aleixo,Adriana Mendes; Fett-Neto,Arthur Germano; Henriques,Amélia T.
This work describes the bioconversion of (-)- and (+)-alpha-pinene (2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene), targeted at the production of (-)- and (+)-verbenone (4,6,6-trimethyl-bicyclo (3.1.1) hept-3-en-2-one), respectively, using Psychotria brachyceras and Rauvolfia sellowii cell suspension cultures. P. brachyceras showed selectivity to (-)-alpha-pinene with 80.9% conversion (relative integrated area gas chromatography-mass spectrometry (GC-MS)) of (-)-verbenone in 10-day-incubation, whereas R. sellowii was able to convert both pinene enantiomers (37.6% conversion of (-)-verbenone in 7-day-incubation and 32.2% conversion of (+)-verbenone in 10-day-incubation). In both systems trans-verbenol was formed as main product and then slowly biocatalyzed to...
Tipo: Journal article Palavras-chave: Alpha-pinene; Biotransformation; Verbenol; Verbenone.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400003
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Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production ArchiMer
Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf
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Effects of short-term exposure to paralytic shellfish toxins on clearance rates and toxin uptake in five species of New Zealand bivalve ArchiMer
Contreras, Andrea; Marsden, Islay D.; Munro, Murray H. G..
Algal blooms produced by toxic dinoflagellates have increased worldwide, resulting in economic losses to aquaculture and fisheries. Bivalve species differ in their ability to feed on toxin-producing dinoflagellates and this could result in differences in toxin accumulation among species. In New Zealand, the effects of paralytic shellfish poisoning (PSP) toxins on the physiology of bivalve molluscs are relatively unknown. We hypothesised that the feeding responses of five New Zealand bivalve species exposed to PSP-toxic dinoflagellates would be species-specific, affecting their accumulation of toxins. Each species was exposed to toxic and non-toxic species of Alexandrium spp. and clearance rate used as an index of sensitivity to PSP toxins. Clearance rates...
Tipo: Text Palavras-chave: Biotransformation; Decarbamoylation; Dosinia anus; Epimerisation; Ostrea chilensis; Paphies donacina; Pecten novaezelandiae; Perna canaliculus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00069/18007/16007.pdf
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Dietary exposure of juvenile common sole (Solea solea L.) to polybrominated diphenyl ethers (PBDEs): Part 2. Formation, bioaccumulation and elimination of hydroxylated metabolites ArchiMer
Munschy, Catherine; Moisan, Karine; Tixier, Celine; Pacepavicius, G.; Alaee, M..
The uptake, elimination and transformation of six PBDE congeners (BDE-28, -47, -99, -100, -153, -209) were studied in juvenile common sole (Solea solea L) exposed to spiked contaminated food over a three-month period, and then depurated over a five-month period. Methoxylated (MeO-) and hydroxylated (OH-) PBDEs were determined in fish plasma exposed to PBDEs and compared to those obtained in control fish. While all MeO- and some OH- congeners identified in fish plasma were found to originate from non-metabolic sources, several OH- congeners, i.e., OH-tetraBDEs and OH-pentaBDEs, were found to originate from fish metabolism. Among these, 4 '-OH-BDE-49 was identified as a BDE-47 metabolite. Congener 4 '-OH-BDE-101, identified here for the first time, may be...
Tipo: Text Palavras-chave: Marine flatfish; PBDEs; Bioaccumulation; Biotransformation; Hydroxylated metabolites.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12811/11295.pdf
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Dietary exposure of juvenile common sole (Solea solea L.) to polybrominated diphenyl ethers (PBDEs): Part 1. Bioaccumulation and elimination kinetics of individual congeners and their debrominated metabolites ArchiMer
Munschy, Catherine; Moisan, Karine; Tixier, Celine; Olivier, Nathalie; Gastineau, Olivier; Le Bayon, Nicolas; Buchet, Vincent.
The uptake and elimination of six PBDE congeners (BDE-28, -47, -99, -100, -153, -209) were studied in juvenile common sole (Solea solea L) exposed to spiked contaminated food over a three-month period, then depurated over a five-month period. The results show that all of the studied PBDEs accumulate in fish tissues, including the higher brominated congener BDE-209. Several additional PBDE congeners were identified in the tissues of exposed fish, revealing PBDE transformation, mainly via debromination. The identified congeners originating from PBDE debromination include BDE-49 and BDE-202 and a series of unidentified tetra-, penta-, and hepta- BDEs. Contaminant assimilation efficiencies (AEs) were related to their hydrophobicity (log K-ow) and influenced by...
Tipo: Text Palavras-chave: Marine flatfish; PBDEs; Bioaccumulation; Biotransformation; Debromination.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00021/13188/11296.pdf
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Contamination des crustacés décapodes par les composes organohalogénés : étude détaille de la bioaccumulation des PCB chez l'araignée de mer Maja brachydactyla ArchiMer
Bodin, Nathalie.
Marine decapod crustaceans are macrobenthic opportunist species, of great commercial interest, and can accumulate pollutants. Worrying about consumers' safety, polychlorobiphenyls (PCBs), polybromodiphenyl Ethers (PBDEs) and dioxins (PCDD/Fs) were analyzed in four crustacean species from the coastal waters of Brittany and Normandy. As regards to regulations, the consumption of crustacean muscle doesn't raise any health problems. The second objective of this study was to evaluate the biological processes influencing PCB bioaccumulation in the spider crab Maja brachydactyla. From a quantitative point of view, organotropism, diet and age were the major factors acting on contamination. From a qualitative point of view, PCB fingerprints depended on sex, and to...
Tipo: Text Palavras-chave: Food safety; Biotransformation; Biomagnification; Food webs; Stable isotopes; Decapod crustaceans; Bioaccumulation; Polychlorinated biphenyls; Organic contaminants; Sécurité sanitaire; Biotransformation; Bioamplification; Réseaux trophiques; Isotopes stables; Crustacés décapodes; Bioaccumulation; Contaminants organiques.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-1149.pdf
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Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa Neotropical Ichthyology
Garcia,Cylene Zambrozi; Martinez,Claudia B. R..
Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L-1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione S-transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Antioxidants; Biotransformation; Cyanotoxin; DNA damage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300015
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Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas Neotropical Ichthyology
Vieira,Carlos Eduardo Delfino; Almeida,Mayara da Silva; Galindo,Bruno Ambrssio; Pereira,Lindalva; Martinez,Claudia Bueno dos Reis.
Aquatic ecosystems in areas with intense agricultural activity are subject to pesticide contamination, which may compromise the health of the fish. In order to verify the quality of the water and the possible effects of pesticides on fish, a method that combines different biomarker responses into an index named "integrated biomarker response" (IBR) was applied using the biological alterations in the Neotropical fish Astyanax altiparanae. Fish were maintained in situ at five sites along a stream that runs in an agricultural area and in a stream within a forest fragment, considered a reference site. After seven days of exposure the following alterations were observed in fish confined at experimental sites: increased activity of glutathione-S-transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax altiparanae; Biomonitoring; Biotransformation; Genotoxicity; Pesticides.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000100153
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Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production Biological Sciences
Deffert, Flávia; Agustini, Bruna Carla; Picheth, Geraldo; Bonfim, Tania Maria Bordin.
Fructooligosaccharides are catalyzed by β–fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L-1 sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v-1), and Saccharomyces...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.12.00.00-9 prebiotics; Biotransformation; Identification; PCR. Microbiologia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34140
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A New Acetylcholinesterase Inhibitor from Green Glycosylation of Trachyloban-19-oic Acid by Mucor plumbeus Anais da ABC (AABC)
SANTOS,GABRIEL F. DOS; TAKAHASHI,JACQUELINE A..
ABSTRACT The in vitro metabolism of a widespread natural product, trachyloban-19-oic acid (1), by the fungal species Mucor plumbeus was studied in a sucrose-yeast liquid medium. Two products were isolated, and their structures were determined by spectroscopic means as 7β-hydroxytrachyloban-19-oic acid (5) and trachyloban-19-O-β-D-glucopyranosyl ester (6). To the best of our knowledge, compound 6 is herein reported by the first time in the literature. These compounds were assayed for acetylcholinesterase inhibition along with some related compounds. Compound 6 showed the highest acetylcholinesterase inhibitory activity at 10000 µg/mL among the tested compounds, a result (92.89%) comparable to the activity of the positive control, galanthamine (94.21%)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase inhibitors; Biotransformation; Green glycosylation; Mucor plumbeus; Trachyloban-19-oic acid.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000501961
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Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus Anais da ABC (AABC)
Ferreira,Nelson R.; Sarquis,Maria Inez M.; Alves,Cláudio N.; Santos,Alberdan S..
The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28"C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l-1 in 120g l-1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Biotransformation; Kojic acid; Secondary metabolite; Fructose-isomerase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004
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Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates BABT
Bier,Mário César Jucoski; Poletto,Sabrina; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Medeiros,Adriane Bianchi Pedroni.
The objective of the present work was to isolate and select strains with potential to perform the biotransformation of terpenic substrates. Microorganisms obtained from a collection culture and also isolated from a natural source of terpene substrate were tested. Seventeen strains were selected by their resistance to terpenes in potato dextrose agar containing up to 1% of limonene or α-pinene and β-pinene (1:1). Subsequently, 10 strains were selected by their capacity of using these terpenes as sole carbon source in a mineral medium. The biotransformation capacity of these strains was tested and the products obtained were identified by GC-MS.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma; Flavour; Limonene; Β-pinene; Biotransformation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500020
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Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation BABT
Guelfi,Marieli; Maioli,Marcos Antonio; Tavares,Marco Aurélio; Mingatto,Fábio Erminio.
ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fipronil; Hepatotoxicity; Bioenergetics; Biotransformation; Calcium homeostasis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600843
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Biotransformation of Benzaldehyde to L-Phenylacetylcarbinol (L-PAC) by Free Cells of Torulaspora delbrueckii in presence of Beta-Cyclodextrin BABT
Shukla,Vilas. B.; Kulkarni,Pushpa R..
Studies were carried out to explore the possibility of decreasing the toxic and inhibitory effects of the substrate benzaldehyde during its biotransformation to L-PAC by free cells of Torulaspora delbrueckii using beta -cyclodextrin (beta -CD). Use of various levels of benzaldehyde and acetaldehyde in presence of 2% of beta -CD showed that, in presence of beta -CD, the organism could tolerate higher levels of benzaldehyde and acetaldehyde. Semi-continuous feeding of benzaldehyde and acetaldehyde was found to increase the yield of L-PAC in comparison with one time feeding.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benzaldehyde; Biotransformation; Cyclodextrin; L-phenylacetycarbinol; Torulaspora delbrueckii.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300003
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Biotransformation of the monoterpene, limonene, by Fusarium verticilloides BABT
Oliveira,Brás Heleno de; Strapasson,Rogério Antônio.
Limonene, the main constituent of the orange peel oil, was used as substrate for the biotransformation by the fungus Fusarium verticilloides. A hydroxylated derivative of limonene was isolated from the culture broth and characterized by spectroscopic methods. It was identified as perillyl alcohol, an important anti-cancer compound.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Perillyl alcohol; Biotransformation; Fusarium verticilloides.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100002
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Production of allyl phenyl carbinol (APC) by biotrans-formation using Rhizopus arrhizus BABT
Shukla,Vilas B.; Kulkarni,Pushpa R..
The objective of the present work was to study the stereoselective synthesis of homo-allylic alcohol using stereoselective hydrolysis by Rhizopus arrhizus and also to study the stereoselective synthesis of allyl phenyl carbinol (APC) or 1-Phenyl-3-butene-1-o by combination of chemical synthesis and biotransformation from cheap raw materials such as benzaldehyde and allyl bromide. Stereospecific synthesis of allyl phenyl carbinol (APC) was achieved by acetate hydrolysis by R. arrhizus giving R (+) enantiomer. Maximum enantiomeric excess of APC was obtained at 16 h where as maximum yield of it was obtained at 48 h of biotransformation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allyl Phenyl Carbinol; Biotransformation; Rhizopus arrhizus.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300001
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Enzymatic systems involved in D-limonene biooxidation BABT
Menéndez,Pilar; García,Carlos; Rodríguez,Paula; Moyna,Patrick; Heinzen,Horacio.
The biooxidation of limonene by an Aspergillus strain resulted in the production of perillyl alcohol and short chain fatty acids. Addition of ketoconazole, a known inhibitor of cytochrome P450 oxydase, eliminated the production of free acids, but did not affect biotransformation to perillyl alcohol.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Biotransformation; Perillyl alcohol; Cytochrome oxydase; Inhibitors.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200001
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Effects of pregnancy and protein-energy malnutrition on monooxygenase O-dealkylation activity in rat liver microsomes BJMBR
Kuriyama,S.N.; De-Oliveira,A.C.A.X.; Fidalgo-Neto,A.A.; Paumgartten,F.J.R..
Xenobiotic metabolism is influenced by a variety of physiological and environmental factors including pregnancy and nutritional status of the individual. Pregnancy has generally been reported to cause a depression of hepatic monooxygenase activities. Low-protein diets and protein-energy malnutrition have also been associated with a reduced activity of monooxygenases in nonpregnant animals. We investigated the combined effects of pregnancy and protein-energy malnutrition on liver monooxygenase O-dealkylation activity. On pregnancy day 0 rats were assigned at random to a group fed ad libitum (well-nourished, WN) or to a malnourished group (MN) which received half of the WN food intake (12 g/day). WN and MN rats were killed on days 0 (nonpregnant), 11 or 20...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytochrome P450; Monooxygenases; Xenobiotic metabolism; Undernutrition; Gestation; Biotransformation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100014
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Whole cells in enantioselective reduction of benzyl acetoacetate BJM
Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da; Souza,Rodrigo Octavio Mendonça Alves de.
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotransformation; Chiral reduction; Β-ketoester; Kluyveromyces marxianus; Immobilization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300023
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