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Registros recuperados: 74
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A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf
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Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in...
Tipo: Text Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf
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Mise au point de techniques d'isolement de parasites - Compte rendu d'activité ArchiMer
Grizel, Henri; Bachere, Evelyne; Gagneraud, Sylvie; Audic, Guylaine.
Les recherches faisant l'objet de ce contrat ont porté sur la mise au point de techniques adaptées aux bivalves marins et à l'étude du parasite Bonamia ostreae responsable de la maladie hémocytaire de l'huître plate, Ostrea edulis.
Tipo: Text Palavras-chave: Pathologie; Huître plate; Ostrea edulis; Bonamia ostreae; Techniques laboratoire.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/00015/12626/9514.pdf
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Contribution à la validation et à l'optimisation de la technique de PCR pour la détection de Bonamia ostreae chez l'huître plate : Ostrea edulis ArchiMer
Touzeau, Damien.
L'ostréiculture représente une part économique régionale et française importante avec une production d'environ 117 106 Tonnes de coquillages par an (FAO, 2003). Cependant le développement de cette activité peut être limité par des mortalités, parfois massives, dues à des maladies infectieuses. L'huître plate Ostrea edulis a subi deux maladies majeures : la Marteiliose due à Marteilia refringens et la Bonamiose due à Bonamia ostreae. Ces maladies à déclaration obligatoires ont fait chuter la production de 20 000 tonnes dans les années 70 à 2 098 Tonnes aujourd'hui. Suite à ces mortalités, différents programmes de recherche ont été lancés par Ifremer pour tenter de préserver voire de relancer la culture de l'huître plate. Un de ces programmes concerne la...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Parasite; Bonamia ostreae; Technique PCR; ARN 18 S; Diagnostic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00034/14478/11777.pdf
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Essai de culture de l'huître plate Ostrea edulis dans le bassin d'Arcachon. Résultats préliminaires ArchiMer
Robert, Rene; Pichot, Yves; Comps, Michel.
From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks. Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously. The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water...
Tipo: Text Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf
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Contrôle des performances des souches d'huîtres plates résistantes au protozoaire Bonamia ostreae 2ème année ArchiMer
Haure, Joel; Nourry, Max; Le Bayon, Nicolas; Baud, Jean-pierre.
Un contrôle des performances de souches d'huîtres plates, Ostrea edulis, issues d'un plan de sélection a été réalisé d'avril à novembre 1995 en élevage intensif. Un débit d'eau de mer fixé à 4l/heure/huître et un apport nutritif (Skeletonema costatum) de 3.10(9) cellules/jour/huître semblent constituer un bon compromis pour satisfaire les exigences physiologiques de l'huître plate. Les lots d'huîtres résistantes ou tolérantes à Bonamia ostreae présentent de meilleures performances de croissance que les lots témoins. Une hiérarchisation de la croissance validée sur le plan statistique a été mise en évidence comme suit : R6 (F0*F1) > R7 (F1*F2) > Q (lot témoin). La plupart des huîtres ont été infestées par le ver Polydora, malgré le traitement à la...
Tipo: Text Palavras-chave: Génétique quantitative; Ostrea edulis; Bonamia ostreae; Souche résistante.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00048/15875/13297.pdf
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La bonamiose de l' huître plate Ostrea edulis. Bilan des connaissances actuelles, analyse des données collectées en France de 1989 à 2004, mesures de lutte et perspectives ArchiMer
Blanchet, Estelle.
Bonamiosis, favoured by change of temperature and stress, causes mortalities in the adult flat oyster Ostrea edulis in the majority of European oyster farmers. The life cycle of this Haplosporidia protozoan Bonamia ostreae is unknown, it is not dttectable during the five mon th latent period. Because of the Jack of sensibility of reference techniques, histology and tissue smear, further use of PCR assays or HCM-CC, in particular, is recommended. Analysis of data collected in France from 1989 to 2004 shows the absence of simple prediction methods and an apparent cyclicity of the disease. Transfer restriction and control of boat hulls can protect uninfected areas. Reducing stress and density, commercialising earlier can limit losses in infected areas. The...
Tipo: Text Palavras-chave: Bivalve; Bonamia ostreae; Epidémiologie; Haplosporidia; Huître plate; Lutte; Ostrea edulis; Protozoaire; Surveillance; Bonamia ostreae; Control; Epidemiology; Flat oyster; Haplosporidia; Ostrea edulis; Protozoan; Survey.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00451/56235/57795.pdf
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La Bonamiose : études des mécanismes cellulaires de défense de l'huître plate, Ostrea edulis, vis à vis du parasite Bonamia ostreae ArchiMer
Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno.
Bonamiosis—a disease caused by a protozoan parasite, Bonamia ostreae—was detected for the first time in Brittany in 1979 in conjunction with a massive mortality event. Since then, the disease has spread to all French oyster farming areas, and then throughout Europe.
Tipo: Text Palavras-chave: Pathologie; Ostrea edulis; Bonamia ostreae.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3241.pdf
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Analyse bibliographique: historique de l'huître plate, Ostrea edulis, et la Bonamiose, maladie due au protozoaire Bonamia ostreae ArchiMer
Cochennec-laureau, Nathalie.
Oyster productions represent an important part of the french aquaculture. However, diseases are considered as the first limiting factors for the development of this activity around the world. Two exemples point out this problem for the french flat oyster production: Marteiliosis, the disease of the digestive gland, caused by Marteilia refringens, and Bonamiosis, the hemocytic disease, caused by Bonamia ostreae. This report proposes a bibliography analysis of major results obtained for Bonamiosis studies.
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Bonamiose; Pathologie; European flat oyster; Oslrea edulis; Bonamia ostreae; Bonamiosis; Pathology.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00105/21661/19242.pdf
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Study of interactions between flat oyster, Ostrea edulis, and the parasite Bonamia ostreae using flow cytometry and suppression subtractrive hybridization (SSH) ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Gagnaire, Beatrice; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. Control of bonamiosis requires a good knowledge of the parasite life cycle including host-parasite interactions. In this study, we investigated interactions between parasites and haemocytes by flow cytometry and Suppression Subtractive Hybridization (SSH). In both approaches, analyses were performed after 2 hours of contact between haemocytes and parasites purified from highly infected flat oysters. Flow cytometry analyses consisted in testing haemocyte activities including esterase activities, reactive oxygen species (ROS) production and phagocytosis after contact with live parasites and parasites inactivated by...
Tipo: Text Palavras-chave: SSH; Suppression subtractive hybridization; Cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3367.pdf
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Evolution de la situation épizootiologique en Bretagne en 1983 suite au développement de Bonamia ostreae ArchiMer
Tige, Gilbert; Grizel, Henri; Cochennec, Nathalie; Rabouin, Marie-agnès.
Bonamia ostreae diseease is always present in Brittany in the different farming areaa except few free natural oysters beds. Yet Bonamiose disease have not increased in the Bay of Cancale on oysters initialy very few parasited. The technical modifications of rearing and the eradication on sick oysters could explain this situation.
Tipo: Text Palavras-chave: France; Bretagne; Pathologie; Parasite; Bonamiose; Bonamia ostreae.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-4971.PDF
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Relance de l'huître plate. Rapport d'avancement des travaux. Année 1992 ArchiMer
Martin, Anne-genevieve.
A revival program for the European flat oyster Ostrea edulis was initiated in Brittany in 1984 and funded by regional and national agencies. Since then, the research programs were carried out and the 1992 results are presented in this report. Disease status and spat settlement trends are described within each breeding area as well as advances in management practices and genetic research.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiologie; Pathologie; Génétique; Echantillonnage; Elevage; Shellfish; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiology; Pathology; Genetic; Sampling; Breeding.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00427/53823/54814.pdf
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In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens ArchiMer
Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric.
The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few...
Tipo: Text Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF
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Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge ArchiMer
Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf
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Selecting flat oysters, Ostrea edulis, for survival against the parasite Bonamia ostreae : assessment of the resistance of a first selected generation ArchiMer
Martin, Anne-genevieve; Gerard, Andre; Cochennec, Nathalie; Langlade, Aime.
In 1989, a selected strain of flat oyster. Ostrea edulis, was produced from breeders that survived Bonamia ostreae and that were over selected by injection of purified parasites. This generation (G 1) was compared to native oysters which had settled during the same year. After a growing period of 21 months, the oysters were tested according to three procedures: exposure to natural infection in an intertidal area, maintenance at the laboratory in tanks near heavily infested oysters, injection of parasites. The test lasted 7 months. In each procedure, oysters of the first generation (G1) showed a better survival rate than control oysters (72 to 94% versus 48 to 66%). On the other hand, the highest mortalities were observed among injected oysters (G1 and...
Tipo: Text Palavras-chave: Genetics; Ostrea edulis; Bonamia ostreae; Parasite resistance.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/acte-3201.pdf
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Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro ArchiMer
Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf
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Population bottleneck and effective size in Bonamia ostreae-resistant populations of Ostrea edulis as inferred by microsatellite markers ArchiMer
Launey, Sophie; Barre, Marc; Gerard, Andre; Naciri, Yamama.
Genetic variability at five microsatellite loci was analysed in three hatchery-propagated populations of the flat oyster, Ostrea edulis. These populations were part of a selection programme for resistance to the protozoan parasite Bonamia ostreae and were produced by mass spawns, without control of the genealogy. Evidence for population bottlenecks and inbreeding was sought. A reduction in the number of alleles, mainly due to the loss of rare alleles, was observed in all selected populations, relative to the natural population from which they were derived. Heterozygote excesses were observed in two populations, and were attributed to substructuring of the population into a small number of families. Pedigree reconstruction showed that these two populations...
Tipo: Text Palavras-chave: Inbreeding; Effective population size; Bonamia ostreae; Ostrea edulis; European flat oyster.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-2703.pdf
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Relance de l'huître plate, Ostrea edulis. Bilan des travaux réalisés en 1993 ArchiMer
Martin, Anne-genevieve.
A revival program for the European flat oyster Ostrea edulis was initiated in Brittany in 1984 and funded by regional and national agencies. Since then, the research programs were carried out and the 1993 results are presented in this report. Disease status and spat settlement trends are described within each breeding area as weil as advances in genetic research.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiologie; Pathologie; Génétique; Echantillonnage; Elevage; Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiology; Pathology; Genetic; Sampling; Breeding.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00427/53822/54813.pdf
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A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf
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Bonamiasis : a model study of diseases in marine molluscs ArchiMer
Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne.
Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!.
Tipo: Text Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf
Registros recuperados: 74
Primeira ... 1234 ... Última
 

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