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| Registros recuperados: 31 | |
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| Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. |
| ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 |
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| Singh,Jagdish; Kaur,Pawandeep. |
| The aim of this work was to isolate the potent bacterial strains for the production of cellulose enzyme. A total 30 bacterial isolates showed positive results for the cellulase production but highest enzyme activity was shown by isolate JS 14. From the morphological and biochemical reactions, the isolate was identified as Bacillus sp. Cellulase production was studied by this strain using response surface methodology (RSM). A central composite design (CCD) quadratic response surface was applied to explicate the parameters that significantly affected cellulase production in solid substrate fermentation (SSF). The wheat bran concentration and incubation period were significant factors. The process parameters optimized with response surface methodology was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Response surface methodology; Optimization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400004 |
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| Chellapandi,P.; Jani,Himanshu M.. |
| Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation. It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endoglucanase; Streptomyces isolates; Submerged cultivation; Media optimization; Cellulase; CMCase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100026 |
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| Avellaneda-Torres,Lizeth Manuela; Pulido,Claudia Patricia Guevara; Rojas,Esperanza Torres. |
| A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Endoglucanase; Β-glucosidase; Exoglucanase; Páramo; High Andean Forest. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400011 |
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| Acharya,Somen; Chaudhary,Anita. |
| Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes. Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the physiology of thermophilic cellulases producers and process development for enzyme production and biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lignocelluloses; Bioethanol; Cellulase; Thermophiles. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300001 |
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| Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing. |
| The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006 |
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| Nunes,Maíra F; Cunha-Santino,Marcela B. da; Bianchini Jr,Irineu. |
| Enzymatic activity during decomposition is extremely important to hydrolyze molecules that are assimilated by microorganisms. During aquatic macrophytes decomposition, enzymes act mainly in the breakdown of lignocellulolytic matrix fibers (i.e. cellulose, hemicellulose and lignin) that encompass the refractory fraction from organic matter. Considering the importance of enzymatic activities role in decomposition processes, this study aimed to describe the temporal changes of xylanase and cellulose activities during anaerobic decomposition of Ricciocarpus natans (freely-floating), Oxycaryum cubense (emergent) and Cabomba furcata (submersed). The aquatic macrophytes were collected in Óleo Lagoon, Luiz Antonio, São Paulo, Brazil and bioassays were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anaerobic decomposition; Aquatic macrophytes; Xylanase; Cellulase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100010 |
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| Moretti,Marcia M. S.; Bocchini-Martins,Daniela A.; Silva,Roberto Da; Rodrigues,André; Sette,Lara D.; Gomes,Eleni. |
| Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thermophilic fungi; Solid-state fermentation; Xylanase; Cellulase. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 |
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| Zanelato,A. I.; Shiota,V. M.; Gomes,E.; Silva,R. da; Thoméo,J. C.. |
| This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3busing a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50ºC), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7:3, 50ºC temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Xylanase; Myceliophthora sp.; Packed bed; Solid state-fermentation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400038 |
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| Luz,José Maria Rodrigues da; Nunes,Mateus Dias; Paes,Sirlaine Albino; Torres,Denise Pereira; Silva,Marliane de Cássia Soares da; Kasuya,Maria Catarina Megumi. |
| The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Cellulase; Mushrooms; Lignocellulolytic residues. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035 |
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| Oliveira,Lilian C.G.; Ramos,Patricia Locosque; Marem,Alyne; Kondo,Marcia Y.; Rocha,Rafael C.S.; Bertolini,Thiago; Silveira,Marghuel A.V.; Cruz,João Batista da; Vasconcellos,Suzan Pantaroto de; Juliano,Luiz; Okamoto,Debora N.. |
| Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Halophilic; Protease; Lipase; Amylase; Cellulase. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200347 |
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| Heck,Júlio X.; Hertz,Plinho F.; Ayub,Marco A.Z.. |
| In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max) protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solid-State cultivation; Cellulase; Xylanase; Soybean residue; Amazon biodiversity. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300005 |
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| Cunha-Santino,Marcela B. da; Bianchini Jr.,Irineu. |
| Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition. Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources added to Óleo lagoon (21º 36'S and 47º 49'W) water maintained in the dark under anaerobic condition at 15ºC, 20ºC, 25ºC and 30ºC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Utricularia breviscapa; Cellulose. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200008 |
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| Registros recuperados: 31 | |
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